BY2012 Microbiology

Gallery of Candida

Candida Art

Candida albicans

Diagram as a budding yeast

Candida albicans and Oral Thrush

Source: Atlas of Clinical Oral Pathology, 1999

Candida albicans and Oral Thrush

Fungal Dimorphism
Candida albicans

Yeast phase normal

flora [Gram stain]

Hyphal phase infection

Yeasts growing at 30C on agar. Filamentous hyphae produced

when growing at 37C in vivo causing invasion of epithelial tissue.

Candida Buccal Epithelial Cells

Yeast cells

Epithelial cell

Hyphae

Candida albicans Infection

nucleus

epithelial cell
hypha

blastospores

Gram-stain of vaginal smear showing Candida albicans and

epithelial cells with many Gram-negative rods in background

Candida albicans

Scanning electron micrograph of C. albicans

germ tube penetrating living tissue (arrowed)

Candida albicans

Pseudohypha
Budding yeast
Phase contrast micrographs of C. albicans

Candida albicans

Wet mount - phase contrast

microscopy

Gram stain

Candida albicans

Stained with fluorescein-labelled antibodies UV microscopy

Candida Features
Budding yeast Wet mount

Budding yeast Gram stain

Germ tube

Chlamydospores

Candida
Pseudohyphae Production

Candida Adherence to Buccal

(Cheek) Epithelial Cells

Candida Cell Surface Features

Negative staining

Freeze etching

The outer cell wall layer is composed mainly of mannoproteins

covalently bound to -1,6- or -1,3-glucan and appears as a
dense network of radially projecting fibrils

Candida Dimorphism

Germ Tubes of Candida albicans

Colourised scanning electron micrograph of

C. albicans yeast cells producing germ tubes

Candida albicans forming

Hyphae on Corn Meal Agar
Yeast cells

Hyphae

Chlamydospores of
Candida albicans (low power)

Chlamydospores of
Candida albicans

Black arrows = Chlamydospores; White arrows= Suspensor cells

Chlamydospores of
Candida albicans
A

White arrows = Chlamydospores

Black arrows = Suspensor cells

Chlamydospores of
Candida albicans

Hypha
Chlamydospores

Stained with Grams stain

Chlamydospores of
Candida dubliniensis

Note multiple chlamydospores on the tips of the suspensor cells (arrowed)

Chlamydospores of
Candida dubliniensis

P = Pseudohypha; S = Suspensor cell

Chlamydospores occur in groups (arrowed)

Candida Identification
BBL CHROM Candida Agar

Candida Identification
BBL CHROM Candida Agar

BBL CHROM Candida Agar

C. dubliniensis

C. tropicalis

C. glabrata

C. albicans

C. parapsilopsis

C. krusei

BBL CHROM Candida Agar

C. albicans

C. krusei

C. tropicalis

C. glabrata

Candida Identification
HARDY CHROM Candida Agar
Candida glabrata

Candida krusei

Candida albicans

Candida tropicalis