BIOL 1010 TakeHome Questions Exam #4 Name_Lauren Skips Answer each question in the space provided You may want to use www.pdfescape.com 4) (4pts) DRAW the leading and lagging strands on the replication fork below. Make sure to show Okazaki Fragments and the ALL the primers. The Lines drawn are the template strands. phosphates Origin of a PLP lagging strand ‘ phosphates 2) (3pts) Explain why we refer to DNA replication as semi-conservative and describe an experiment that showed it is semi-conservative. Itis semi-conservative because when the DNA is replicated one strand is the same from the old strand and one is a completely new strand of DNA. The Meselson and Stahi experiment showed the semi conservative form of DNA by observing DNA is different solutions and seeing the band weight difference.3) (pts) Why did Rosalind Franklin's x-ray photograph of DNA help Watson and Crick discover the structure of the molecule? Her work really changed the study of DNA. She was able to take an X-ray photo of DNA revealing that it was a double helix shape. 4) (2pts) Watson provided the names to the processes of Transcription and Translation. Explain why he called them that. Its transcription because you are still working with nucleic acids just changing the code where as the translation process requires you to move from nucleic acids to amino acids 5) (2pts) Explain how the R bacteria in Griffith's Transformation experiment became S bacteria. The R bacteria could be killed but the DNA from the R bacteria was not and was transformed into the S bacteria which was a new bacteria but had the R bacteria DNA.6) (3pts) DESCRIBE the following techniques. Gel Electrophoresis Molecules are separated based by their size using an electric charge Southern Blotting Using the results from the Gel electrophoresis you then place the gel on a special blot paper to transfer the data onto a nylon membrane. Polymerase Chain Reaction Polymerase chain reaction is when the primers ere combined with polymerase and deoxynucleotides to amplify DNA sequences. 7) (2pts) Outline the role of the aminoacy! tRNA synthetases (the Synthetases) in Translation The aminoacy| is in charge of reading the codon of the tRNA and attaching the right amino acid to the specific tRNA.