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C-Reactive Protein
C-Reactive Protein
C-Reactive Protein
2. HBV: 50 microliters
3. HIV: 25 microliters
Controls:
1. Treponema
Specific ID:
1. HBV: 51
2. HIV: 53
3. HCV: 55
Reagents:
1. Lyophilized soln contained in
the amber bottle
stability: 1 month
2. Washing soln
volume: 2L
dilution: 1:20 (1.9L of
dH2O & 100 mL of
washing soln)
stability: 1 week at RT/ 2
weeks in ref
3. Conjugate detects Ag & Ab
2 conj. are used for HCV
& HIV tests
4. Color developer
substrate+ chromogen
dilution: 1:11 (10 mL
subs. & 1 mL chromo.)
stability: 6 hrs.
5. Stopping soln
1N H2SO4
2. HIV
Volume of Sample:
1. Trep. & HBV: 100 microliters
2. HIV: 75 microliters
3. HCV: 50 microliters
Volume of Conj.:
1. HCV: 100 microliters
3. HBV
Procedure
1.
2.
3.
4.
4. HCV
NOTE: preparation of Ag
ctrl. must be done