Yale SCHOOL OF MEDICINE Department of Dermatology KEITH CHOATE, MD, PHD, FAAD Acs Profesor of Dermatsloy, Gentes ‘and Pathology 0 Box 8059 New Haven CT 06520-8059 10378-3912 keith cote yale mmediineyaleedujdermatology ‘Choy Maine December 20, 2014 and Pediatrics (LMP) Re: Work Certificate for Muhammad Nasir Netter roto To whom it may concer: This is to certify that Muhammad Nasir has successfully completed a six month internship at my laboratory in the Department of Dermatology at Yale University from July to December, 2014. Muhammad came to our group to study a cohort of subjects he had collected in Pakistan, mobilizing clinical resources to assemble an interesting group of subjects. In fact, he had already done considerable ‘work on these patients prior to coming to the lab, performing linkage analysis and pre-screening for mutations, While here, he used clinical phenotypes to inform further screening of candidate genes, identifying causative mutations in ~80% of patients. In four, he performed whole exome sequencing analysis, finding a fascinating mutation in RSPOI which causes sex reversal and skin disease. His analysis continues in three other kindreds and holds the potential to identify new genetic causes of skin disorders. In separate work, Muhammad has expressed interest in learning biological techniques utilized in functional characterization of identified mutations. He has become proficient in primary culture of keratinocytes and fibroblasts, the generation of skin equivalents, and retroviral delivery of tetracyeline- regulated genes into primary keratinocytes. He has also become proficient in targeted mutagenesis, immunostaining, Western blotting, and skin grafting in mouse models. He has done important work on a novel, severe variant of Keratitis Ichthyosis Deafness (KID) syndrome which I expect will also lead to publication. During his time here, Muhammad participated in weekly laboratory meetings and departmental seminars as well as in visiting professor lectures at the University. He presented his work effectively and was a valuable contributor to our conversations. thas been a real pleasure working with Muhammad who will bring new technologies home with him. 1 hope that we will be able to continue to collaborate on this fascinating group of disorders. | fully expect that he will have a productive career with adequate institutional support. Please do not hesitate to contact me if | can provide further information. Best wishes, ht, G. Chests Keith A. Choate, M.D., Ph.D. Associate Professor of Dermatology, Genetics, and Pathology Yale University School of Medicine