MEDT 105

HEMATOLOGY LAB
A. Pre-examination Phase
1. Requisition form
2. Blood Collection Techniques
a. Venipuncture
b. Capillary Puncture
3. Order of draw
4. Inappropriate Sites for Blood Collection
5. Problematic Situations
6. Submission and Receipt of Specimen
7. blood smear preparation (manual and automated)
a. thick and thin smear preparation
b. peripheral blood smear preparation
B. Examination Phase
i. identification of mature and immature blood cells
ii. PBS grading
iii. PBS reporting
8. complete blood count (manual and automated)
a. red blood cell count
b. white blood cell count
c. haemoglobin determination
d. haematocrit determination
e. platelet count
f. differential count
g. rbc indices
i. MCV
ii. MCHC
iii. MCH
iv. RDW
9. reticulocyte count
10.
erythrocyte sedimentation rate
11.
osmotic fragility test
12.
Sickle cell tests
13.
special hematology tests
C. Post-Examination Phase
14.
15.
16.

Validating results
Retesting specimens
Referring results

17.
18.

Releasing reports
Retention of Specimens and Documents

D. Quality Assurance
Requirements:
Syringes (3 or 5 cc)
ETS
Capillary tube (heparinized or plain)
Clay and paraffin wax (sealant)
1 box glass slides
Cover slip
Alcohol pads
Micropore
Cotton
10-20 used or washed plain test tubes (5 ml)
Wbc pipet
Rbc pipet
Hemocytometer
1 yellow quiz notebook
Grading System
20%
20%
10%
10%

laboratory activity
quizzes
long exam
attendance

References

Dela Calzada, G. J., Faundo, A. C., Orillaza, M. A., Liu, E. B. (2012). PCQACLs
Standardization and Harmonization of Complete Blood Count in the Philippines.
Quezon City : C & E Publishing
Brown, B. A. (1993). Hematology Principles and Procedures
Internet Sources