Hola te paso un ejemplo espero te funcione

Private Mi_Reporte As New ReportDocument

Private Sub ConfigureCrystalReports()
Dim reportPath As String = Server.MapPath("CryRptCobranzat.rpt")
Mi_Reporte = New ReportDocument()
Mi_Reporte.Load(reportPath)
Dim myConnectionInfo As ConnectionInfo = New ConnectionInfo()
myConnectionInfo.ServerName = ".\SQLEXPRESS"
myConnectionInfo.DatabaseName = "BD"
myConnectionInfo.UserID = "user"
myConnectionInfo.Password = "pasw"
SetDBLogonForReport(myConnectionInfo, Mi_Reporte)
With CRVW_detcorgdelearning
.ReportSource = Mi_Reporte
.SelectionFormula = "{TC_RUTAS_HM.rut_id_num} = 14"
.RefreshReport()
End With
End Sub
Private Sub SetDBLogonForReport(ByVal myConnectionInfo As ConnectionInfo, ByVal
myReportDocument As ReportDocument)
Dim myTables As Tables = myReportDocument.Database.Tables
For Each myTable As CrystalDecisions.CrystalReports.Engine.Table In myTables
Dim myTableLogonInfo As TableLogOnInfo = myTable.LogOnInfo
myTableLogonInfo.ConnectionInfo = myConnectionInfo
myTable.ApplyLogOnInfo(myTableLogonInfo)
Next
End Sub
Protected Sub Page_Load(ByVal sender As Object, ByVal e As System.EventArgs)
End Sub

Protected Sub Page_Init(ByVal sender As Object, ByVal e As System.EventArgs)

ConfigureCrystalReports()
End Sub

Protected Sub Page_Unload(ByVal sender As Object, ByVal e As System.EventArgs) Handles

Me.Unload
Mi_Reporte.Close()
Mi_Reporte.Dispose()
End Sub