ee PSNI CRGRSE POO Va tantra Sangean ne Minitest 3, Nov 20 YP seudent #: APSA ) Colin | Melissa | Tim 1, In yeast, functional (wild type) copies of both MALI and MAL2 genes are needed for cells to grow on medium where maltose is the only sugar. A diploid strain is heterozygous for loss of function mutations in both genes; the mutant alleles mall and mal? are cach recessive to wild type. The diploid was allowed to undergo meiosis; many tetrads were collected and the spores tested for growth on maltose plates. Overall, three tetrad patterns (i ii and iii below) were observed. The growth pattern for just tetrad (i) is filled in by shading, just the colony outlines are shown for the others. Tetrad (i) Genotype? ‘Genotype? Tetrad (iii) Genotype? MeL so @ vauner © weINA® =— Qa mals mal2 @ uae? © ppimal2 © martmai2 i OO matama2 © mavLNArr O Malzmalt Ma O watigan © morimai2 Oo MaAmalt Abundance (fr 1.4): _ ANG _¥ Seuescriaties Leds (a) Give the spore genotypes for Tetrad (i, Then, shade Tetrads (i) and (iil) as necessary to indicate the patterns that must have been observed (ie., how many spores grew vs. didn’t grow on maltose), giving spore genotypes for all spores. (1072) (b) If the two genes are unlinked, what fraction of the spores do you expect would be able to grow on maltose plates? 2m — © OSSoY*+ Independant t4- wR ZI Pe. -need MALL % MALZ () Overall, 200 spores were tested and 8¢ of them proved capable of growth on maltose plates. Based on this result... m0) 49% + Give the complete genotype of the diploid strain above, including phase if appropriate: MpLt MALL malt mald + Ifthe two genes are linked, draw a map to show the distance between them. Show your calculation. If they are not linked, state how you reached that conclusion. Linked. MALL gy MAL2 i =8cM, yah had MALIMAL?, which means i was liFely 4 ca parental in. Trot means 42° were nmj4mal2; F MOA NO. 40.412} 0.1 /2* 0.08 wan-Amal 7; 0.DEmaIdMALZ (d) Fillin the “Abundance” line below the tetrad diagram to intlicate which tetrad type would be the most abundant and which would be least abundant; or write “Equal” if you think all three would be equally abundant. 20) Genome 371 Auton 2015, Minis 3 1 yr2. The pedigree shows inheritance of an autosomal recessive tooth enamel disorder. The tooth enamel gene is linked to a polymorphic microsatellite locus at a distance of 12 eM. The gel image shows the result of a PCR test on each individual for the linked microsatellite marker; each person’s test result is shown directly below that person. Te a a oN 4 = | 4 a Om OD-»»n foe T ao tt OE eee Ferale Male a TH | [LE] ceroypes itn pase) +5 +1 iof= = 5 24 - = — = 4 3 3 — 3 2 | =— 2 we NS - _- Ss hh (a) Fillin the genotypes for Il] and II-2 for the tvo loci, including phase if known. 4s) (b) What is the probability that the first child of I-1 and I-2 will have the tooth enamel disease and Q havea genotype of {4,5} at the polymorphic site? Justify your answer by giving the egg and sperm genotypes and probabilities, and whether each is parental-type or recombinant. j=) =a 99, needs £9 28 ee poremtat> P(E? = 0 4H sperm needs repmbinant PPC) = 0.0l6 3. The pedigree shows P(E, 53) = (ONY) CO. wey inheritance of corte) | tev hemophilia and red- ea ene) | tl Hl 2 Ahaha 2 = Hobe (V @ Give the genosypes 1 (including phase if 3 enn t known) for all wily individuals except 1 Cs TIL, using ? if ‘7 % necessary. (12>) xn ea yon | 4 x A restriction map of allele D is shown to the right of the pedigree. The middle HindIII site is known to be missing in allele d. DNA obtained from TII-] was eutioampleien with HindIII and tested by Southern blot analysis using the indicated probe. Based on the data, give the complete ernooge of HEI. 0) Genome 371 Auiunin 2015, Minis 3