<Fluorescein >

/
(chemiluminescence) , .
(Lab JuniorTM)

0 . !
.
(fluorescence)
.
. (phosphorescence)

. .
.
(fluorite, : CaF ) .

< 1>
Fluorescein 520 nm
.
.
CHO .

< 2> fluorescein

 fluorescein resorcinol( CHO ) phthalic anhydride( CHO )

.

phthalic anhydride

resorcinol

fluorescein

phthalic anhydride resorcinol

fluorescein .
.

fluorescein ,
. (- OH ) (- COO -)
<2> .
fluorescein , dibasic

NaOH proton .

< 3> fluorescein dibasic ion

520 nm
.

Rhodamine , ,
.
. Rhodamine Rhodamine 101
.

< 4> Rhodamine

Rhodamine fluorescein .
.

fluorescein
.
Rhodamine
.

0. (Lab Junior)
OS (Visual Spectra 2.1 Jr.TM) .(32bit OS
, 64bit OS 'Setup'
'Drivers' 'SV2100_Driver_Win7x64_Setup' . OS

)
(light source) (sample holder), (detector, SV2100)
. ()
.( 30 data
)
USB 32bit OS
SV2100
.

1. Fluorescein
50 mg phthalic anhydride .
75 mg resorcinol .
.
5 60 .
.
.
.
100 mL (A) .
A 3mL M NaOH 3 mL dibasic ion (B) .
Rhodamine 101 (C) (A B C)
.
2.
Visual Spectra 2.1 Jr.TM START' .
'Off'
'DARK ' .
.
'On' 'Reference '
.
A .

.
B C .
()
.
,
.
'START' 'File''Open'
DARK REFERENCE .
C
. integration time
(C 60 m sec ).
. 100 m sec
.
A B .
() .


. .
.

(Postech)
.

phthalic anhydride resorcinol
.
fluorescein NaOH .
fluorescein .
,
.
- fluorescein dibasic ion ?
( )
- fluorescein Rhodamine , ?