450

449
448
447
446
445
444
443
442
441
440
439
438
437
436
435
434
433
432
431
430
429
428
427
426
425
424
423
422
421
420
419
418
417
416
415
414
413
412
411
410
409
408
407
406

4.92805
5
5
5
5
5
4.90457
5
4.97921
5
4.8216
4.80476
4.69324
4.76987
4.72093
4.73617
4.65301
4.67941
4.60458
4.54077
4.53099
4.56102
4.66481
4.75196
4.97921
5
5
5
5
5
4.46732
4.50209
4.53011
4.47576
4.44368
4.44368
4.42058
4.41314
4.38461
4.39157
4.38461
4.42815
4.42815
4.4199
4.43584

Spectru UV-VIS al extractului de

6
5
4

Absorbana

3
2
1
0
150

200

250

300

Lungimea de

405
404
403
402
401
400
399
398
397
396
395
394
393
392
391
390
389
388
387
386
385
384
383
382
381
380
379
378
377
376
375
374
373
372
371
370
369
368
367
366
365
364
363
362
361

4.46808
4.49394
4.48515
4.47653
4.43584
4.47653
4.43584
4.49394
4.51207
4.59328
4.59328
4.62702
4.62702
4.58127
4.58127
4.61513
4.72093
4.59226
4.70621
4.61513
4.75196
4.65185
4.61513
4.52057
4.58127
4.58127
4.72093
4.62702
4.59226
4.58127
4.62702
4.57054
4.61513
4.60354
4.63813
4.06817
4.17164
4.16364
4.21146
4.17979
4.26473
4.26473
4.27484
4.21997
4.2056

360
359
358
357
356
355
354
353
352
351
350
349
348
347
346
345
344
343
342
341
340
339
338
337
336
335
334
333
332
331
330
329
328
327
326
325
324
323
322
321
320
319
318
317
316

4.20106
4.24837
4.25298
4.26377
4.26951
4.33207
4.38399
4.52057
4.4199
4.33151
4.23305
4.2847
4.31892
4.22777
4.19534
4.15691
4.23261
4.35024
4.29632
4.24335
4.17357
4.08202
4.04632
4.03076
3.99915
4.03104
4.06545
4.17706
4.2567
4.22255
4.05918
4.02169
3.97626
3.99274
3.9897
4.03692
3.99606
3.98743
3.9358
4.02799
4.11853
4.3293
4.2269
4.18136
4.18136

315
314
313
312
311
310
309
308
307
306
305
304
303
302
301
300
299
298
297
296
295
294
293
292
291
290
289
288
287
286
285
284
283
282
281
280
279
278
277
276
275
274
273
272
271

4.19982
4.27827
4.22299
4.17744
4.12504
4.14881
4.20353
4.19049
4.21357
4.19534
4.33486
4.2614
4.22646
4.1986
4.14409
4.18969
4.19819
4.39994
4.46885
4.54167
4.86111
5
5
5
5
5
4.47653
4.17473
3.96848
4.03889
3.97235
4.1682
4.03635
4.06636
3.9338
3.92981
3.85643
3.85144
3.87234
3.83644
3.80825
3.88567
3.8708
3.95778
3.9153

270
269
268
267
266
265
264
263
262
261
260
259
258
257
256
255
254
253
252
251
250
249
248
247
246
245
244
243
242
241
240
239
238
237
236
235
234
233
232
231
230
229
228
227
226

3.8352
3.71971
3.70555
3.72724
3.88092
3.91894
3.90707
3.87895
3.86375
3.93916
3.95311
4.00724
3.97626
3.89127
3.9025
3.92044
4.0706
4.08991
4.27925
4.2842
4.3686
4.47498
5
5
5
5
5
4.41114
3.69426
3.25456
3.16398
3.01976
2.8643
2.61754
2.37643
2.13957
2.07348
1.92605
2.01228
1.97004
1.9144
1.76815
1.64705
1.59656
1.56309

225
224
223
222
221
220
219
218
217
216
215
214
213
212
211
210
209
208
207
206
205
204
203
202
201
200
199
198
197
196
195
194
193
192
191
190

1.68175
1.36829
1.27194
1.30467
1.29043
1.32502
1.05534
1.06492
1.12782
1.12086
1.16482
1.16576
1.17939
1.06522
0.96989
0.93564
1.02237
1.01284
1.0278
1.02302
1.08922
1.18467
1.03069
1.00944
0.99631
1.11205
1.30394
1.22303
0.99929
0.9017
1.01161
1.08207
1.23546
1.14216
1.05496
1.10123

UV-VIS al extractului de piper, obinut prin procedeul Soxhlet

200

250

300

350

Lungimea de und, nm

400

450

500

450
449
448
447
446
445
444
443
442
441
440
439
438
437
436
435
434
433
432
431
430
429
428
427
426
425
424
423
422
421
420
419
418
417
416
415
414
413
412
411
410
409
408
407
406

5
5
5
5
5
5
5
4.95288
5
5
5
5
5
4.90457
4.76835
4.66601
4.61619
4.60458
4.64039
4.69324
4.67941
4.75343
4.90666
5
5
5
5
5
5
5
4.50209
4.59226
4.65185
4.60354
4.61513
4.60354
4.59226
4.60354
4.53987
4.57054
4.50209
4.50209
4.47576
4.52057
4.55078

Spectru UV-VIS al extractului de piper, ob

6
5
4
Absorbana

3
2
1
0
150

200

250

300

350

Lungimea de und

405
404
403
402
401
400
399
398
397
396
395
394
393
392
391
390
389
388
387
386
385
384
383
382
381
380
379
378
377
376
375
374
373
372
371
370
369
368
367
366
365
364
363
362
361

4.65301
4.65301
4.80312
4.78697
4.82332
4.67818
4.65185
4.69197
4.78539
4.8427
4.80312
4.76835
4.72093
4.8409
4.8409
4.97921
4.8823
4.8823
4.8409
4.8823
4.95288
5
4.97921
5
5
5
5
5
5
5
5
5
4.90457
4.76835
5
4.07766
4.09086
4.13552
4.10152
4.22996
4.24426
4.35784
4.26951
4.24335
4.29073

360
359
358
357
356
355
354
353
352
351
350
349
348
347
346
345
344
343
342
341
340
339
338
337
336
335
334
333
332
331
330
329
328
327
326
325
324
323
322
321
320
319
318
317
316

4.20106
4.32599
4.37715
4.48436
4.37041
4.24335
4.2857
4.27435
4.33823
4.27435
4.3903
4.31946
4.3903
4.38274
4.34334
4.27925
4.54986
4.49313
4.67695
4.59024
4.09182
4.14014
4.04661
4.12608
4.0495
4.09246
4.04892
4.06186
4.05534
4.01253
4.02414
4.06216
4.11785
4.26188
4.27729
4.23128
4.08832
4.04288
4.07182
4.10679
4.11011
4.09924
4.06186
4.1861
4.28371

315
314
313
312
311
310
309
308
307
306
305
304
303
302
301
300
299
298
297
296
295
294
293
292
291
290
289
288
287
286
285
284
283
282
281
280
279
278
277
276
275
274
273
272
271

4.40125
4.35962
4.32163
4.34677
4.27241
4.25159
4.15247
4.07859
4.05563
4.0789
4.16478
4.15616
4.11346
4.15616
4.29377
4.51376
4.82505
4.4524
4.49475
4.56102
5
5
5
5
5
5
5
4.58424
4.31892
4.08959
4.04031
4.02772
3.99071
3.95591
3.85662
3.81597
3.84541
3.80377
3.83202
3.77201
3.79594
3.71903
3.74328
3.76367
3.91339

270
269
268
267
266
265
264
263
262
261
260
259
258
257
256
255
254
253
252
251
250
249
248
247
246
245
244
243
242
241
240
239
238
237
236
235
234
233
232
231
230
229
228
227
226

3.88467
3.86908
3.82091
3.79984
3.8131
3.82401
3.81869
3.85273
3.84851
3.77524
3.92152
3.93335
4.14158
4.07643
4.06515
3.95802
3.98567
4.00514
4.36558
4.48673
5
5
5
5
5
5
5
3.97114
3.51766
3.51706
3.21821
2.95176
2.97192
2.60009
2.53673
2.26013
2.14983
1.9826
1.76091
1.68237
1.70559
1.74467
1.56405
1.54074
1.53728

225
224
223
222
221
220
219
218
217
216
215
214
213
212
211
210
209
208
207
206
205
204
203
202
201
200
199
198
197
196
195
194
193
192
191
190

1.63351
1.62925
1.47232
1.46934
1.33187
1.28643
1.37067
1.15113
1.07574
0.97862
1.11028
1.2213
1.46457
1.48845
1.31613
1.39705
1.4414
1.45709
1.2196
1.23874
1.40163
1.38686
1.2363
1.01404
1.10102
1.05219
1.04347
1.1228
0.93938
1.14007
1.03249
1.02536
0.9855
0.95302
0.9655
0.90182

tractului de piper, obinut prin extracie la cald

50

200

250

300

350

Lungimea de und, nm

400

450

500

450
449
448
447
446
445
444
443
442
441
440
439
438
437
436
435
434
433
432
431
430
429
428
427
426
425
424
423
422
421
420
419
418
417
416
415
414
413
412
411
410
409
408
407
406

2.57345
2.60925
2.66646
2.72807
2.78718
2.84807
2.90526
2.96755
3.02957
3.08186
3.13189
3.17295
3.21442
3.2561
3.29936
3.35637
3.40735
3.46566
3.52549
3.59358
3.65982
3.73631
3.81784
3.91594
3.99812
4.07182
4.09567
4.09567
4.06156
4.00045
3.72366
3.70752
3.70752
3.69478
3.69465
3.68905
3.69606
3.71364
3.72257
3.72257
3.70766
3.70489
3.71646
3.72545
3.73308

Spectru UV-VIS al extractului de piper

5
4
3

Absorbana 2
1
0
150

200

250

300

Lungimea de u

405
404
403
402
401
400
399
398
397
396
395
394
393
392
391
390
389
388
387
386
385
384
383
382
381
380
379
378
377
376
375
374
373
372
371
370
369
368
367
366
365
364
363
362
361

3.74543
3.74543
3.75167
3.75505
3.75021
3.75021
3.76987
3.76805
3.77293
3.74673
3.73886
3.72052
3.7342
3.73871
3.745
3.73406
3.73871
3.72339
3.71431
3.70991
3.68829
3.69401
3.65617
3.65758
3.63847
3.63723
3.63589
3.6321
3.60375
3.59572
3.5718
3.55914
3.56319
3.56633
3.55699
3.73322
3.78114
3.82992
3.83167
3.83715
3.82922
3.81394
3.83485
3.85886
3.87974

360
359
358
357
356
355
354
353
352
351
350
349
348
347
346
345
344
343
342
341
340
339
338
337
336
335
334
333
332
331
330
329
328
327
326
325
324
323
322
321
320
319
318
317
316

3.87138
3.89289
3.91829
3.88368
3.88786
3.91297
3.92217
3.877
3.8436
3.82435
3.84162
3.83378
3.80147
3.76038
3.76382
3.79497
3.78951
3.80758
3.71849
3.71971
3.73985
3.64999
3.64368
3.67608
3.74443
3.80692
3.79255
3.75711
3.74789
3.74789
3.73999
3.74185
3.76593
3.76593
3.75756
3.75608
3.76744
3.75593
3.75431
3.78808
3.81142
3.82401
3.79111
3.81801
3.85126

315
314
313
312
311
310
309
308
307
306
305
304
303
302
301
300
299
298
297
296
295
294
293
292
291
290
289
288
287
286
285
284
283
282
281
280
279
278
277
276
275
274
273
272
271

3.84723
3.82418
3.81835
3.82177
3.82765
3.84906
3.87196
3.83733
3.82974
3.79675
3.8041
3.81835
3.84924
3.85942
3.94757
3.96513
3.95451
3.94895
3.96921
3.99581
4.09278
4.22255
4.22169
4.56576
4.14337
3.85606
3.63622
3.60469
3.59972
3.69529
3.59582
3.57724
3.46039
3.4199
3.39132
3.38324
3.37066
3.40689
3.39845
3.39529
3.38217
3.34522
3.38745
3.41268
3.43072

270
269
268
267
266
265
264
263
262
261
260
259
258
257
256
255
254
253
252
251
250
249
248
247
246
245
244
243
242
241
240
239
238
237
236
235
234
233
232
231
230
229
228
227
226

3.4362
3.38537
3.41523
3.447
3.50755
3.5002
3.52601
3.4719
3.56939
3.59267
3.67498
3.61716
3.62637
3.62615
3.61534
3.61353
3.59654
3.63232
3.67929
3.68879
3.68377
3.76367
3.7314
3.83414
3.76563
3.47855
3.2567
3.08547
2.95425
2.70675
2.59779
2.38916
2.21097
2.13544
2.03343
1.94179
1.72841
1.65627
1.48988
1.40409
1.31483
1.26923
1.26021
1.23889
1.09316

225
224
223
222
221
220
219
218
217
216
215
214
213
212
211
210
209
208
207
206
205
204
203
202
201
200
199
198
197
196
195
194
193
192
191
190

0.94966
0.87555
0.90488
0.89627
0.901
0.82065
0.75019
0.68959
0.71355
0.71138
0.68737
0.69592
0.70873
0.76175
0.74801
0.71765
0.60126
0.57498
0.58505
0.69552
0.70161
0.71549
0.67741
0.69146
0.6446
0.64451
0.62275
0.57747
0.57401
0.57602
0.60901
0.60826
0.58592
0.5876
0.57692
0.54177

l extractului de piper, obinut prin macerare

00

250

300

350

Lungimea de und, nm

400

450

500

450
449
448
447
446
445
444
443
442
441
440
439
438
437
436
435
434
433
432
431
430
429
428
427
426
425
424
423
422
421
420
419
418
417
416
415
414
413
412
411
410
409
408
407
406
405
404
403

1.66277
1.69188
1.73658
1.78673
1.83806
1.8901
1.94243
1.99306
2.04357
2.09185
2.14125
2.18878
2.23679
2.28462
2.33463
2.39228
2.45427
2.52698
2.60767
2.706
2.81869
2.9464
3.08396
3.23341
3.38311
3.52793
3.64414
3.7271
3.74428
3.75255
3.68065
3.68615
3.69171
3.69031
3.69877
3.69465
3.68102
3.69209
3.6893
3.70032
3.69057
3.70332
3.70752
3.70032
3.6989
3.70607
3.70885
3.73744

Spectru UV-VIS al extractului de piper, obinu

5
4
3

Absorbana 2
1
0
150

200

250

300

Lungimea de

402
401
400
399
398
397
396
395
394
393
392
391
390
389
388
387
386
385
384
383
382
381
380
379
378
377
376
375
374
373
372
371
370
369
368
367
366
365
364
363
362
361
360
359
358
357
356
355

3.74688
3.75505
3.7314
3.72973
3.739
3.74371
3.7549
3.72807
3.71297
3.70568
3.72339
3.72627
3.74486
3.73084
3.70542
3.67744
3.67327
3.68427
3.67339
3.66673
3.63836
3.63221
3.63466
3.62856
3.62626
3.60961
3.60825
3.58344
3.57695
3.54775
3.54167
3.53091
3.65046
3.67744
3.69388
3.74142
3.75211
3.80328
3.80642
3.81377
3.80279
3.79707
3.83609
3.82453
3.8384
3.82643
3.81142
3.81327

354
353
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345
344
343
342
341
340
339
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331
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329
328
327
326
325
324
323
322
321
320
319
318
317
316
315
314
313
312
311
310
309
308
307

3.75919
3.76578
3.77927
3.81344
3.80394
3.78618
3.74774
3.70792
3.70097
3.71849
3.7321
3.69786
3.661
3.63544
3.68766
3.71903
3.72366
3.66721
3.69709
3.70371
3.6478
3.66053
3.66613
3.69171
3.6767
3.67904
3.72311
3.69414
3.71565
3.70097
3.74313
3.74156
3.76412
3.71552
3.71257
3.73168
3.75036
3.82904
3.84705
3.80493
3.78068
3.7737
3.76896
3.80361
3.79675
3.82609
3.78776
3.77416

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305
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303
302
301
300
299
298
297
296
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279
278
277
276
275
274
273
272
271
270
269
268
267
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263
262
261
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259

3.77232
3.77079
3.82974
3.82211
3.81428
3.77324
3.76142
3.82453
3.84979
3.88686
3.93269
4.05888
4.02634
3.96061
3.85458
3.84108
3.75182
3.65429
3.56824
3.49
3.43918
3.44548
3.47498
3.44017
3.35216
3.25745
3.2509
3.24195
3.27363
3.30111
3.32217
3.40262
3.42448
3.43634
3.43289
3.43953
3.43911
3.41489
3.43592
3.46938
3.46717
3.48936
3.48483
3.48145
3.4763
3.4904
3.49048
3.49337

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231
230
229
228
227
226
225
224
223
222
221
220
219
218
217
216
215
214
213
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211

3.5317
3.63578
3.71538
3.68829
3.60044
3.63432
3.6522
3.69057
3.64028
3.61587
3.53808
3.45475
3.39259
3.41054
3.15743
2.99514
2.89378
2.76826
2.59187
2.43958
2.28042
2.03024
1.82316
1.7162
1.57806
1.53474
1.39682
1.43296
1.34595
1.28964
1.12448
1.05223
1.01395
1.02958
0.91094
0.81027
0.74778
0.70483
0.66717
0.64616
0.60957
0.58191
0.62117
0.5866
0.57904
0.58732
0.59535
0.60172

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200
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0.61358
0.6015
0.61212
0.60889
0.64843
0.58929
0.61006
0.52908
0.56218
0.53916
0.55568
0.56629
0.56112
0.58604
0.54387
0.57533
0.51101
0.49472
0.45607
0.48084
0.5078

ctului de piper, obinut prin cromatografie pe coloan

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Lungimea de und, nm

400

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500