ELISA

Enzyme Linked Immunosorbent

Assay


Antibody

 -
Antigen

.
Immunoassay

) ,, ,(
Antigen Antibody .

 -
Analyte

Analyte Antibody antigen

 Antigen
Antigen:
.
:HCG
,
.
.


Antibodies
Antigens
Specific Affinity
.
antigenic
. determinant

S hortcut to image004.lnk




Antibody
)(Antigen
Antibody .
Antibody polyclonal
.

 -

Antibody
monoclonal Antibody
cell line

.

 ELISA

.
Antibody
.Antigen

 ELISA
:
-1 Competitive ELISA
-2 Sandwich ELISA
Direct ELISA

-3 Indirect ELISA

Competitive ELISA
Antigen
Labeled Antigen
Antigen
. labeled Antigen

Sandwich ELISA
Antigen

. Antibody

Sandwich ELISA- Cont

-1 Antigen .
-2 .
- 3
.

Sandwich ELISA- Cont

- 4
.
-5
Antigen .

Indirect ELISA
Antibody
:
-1 Antigen
.
- 2 Antibody
.primary Antibody

Indirect ELISA- Cont

-3 Antibody Antigen
.
-4 Antibody .
-5 Antibody Secondary
Antibody
Primary Antibody .

Indirect ELISA- Cont

-6 Antibody .
-7 Antibody
.

 ELISA


Kit
) Kit
( Kit.

 -
ELISA
-1 96 )( wells
wells
.Standard curve

 -
ELISA
-2
Duplicate or
Triplicate .
-3 Quality control sample
) Kit (

.




x-axis concentration
y-axis . Absorption
Absorption
nm

Concentration ng/ml

-
standards
x-axis
y-axis .

-



.
Absorption
nm

Concentration ng/ml

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irect-enzyme-linked-immunosorbent-assay-el
isa
ELISA
http://www.sumanasinc.com/webcontent/anima
tions/content/ELISA.html
http://www.biology.ualberta.ca/facilities/mul
timedia/uploads/procedures/elisa-sound.swf