ABSTRACT

The method of choice for analysis of drugs in multi-component

preparations is chromatographic based technique such as high performance liquid
chromatography (HPLC). However, chromatographic method is time consuming
and requiring much effort. As a consequence, some simple methods such as Uv
spectrophotometry are continuously developed, especially in combination with the
chemometrics software. UV-vis spectrophotometry coupled with multivariate
calibration of partial least square (PLS) has been developed for quantitative
analysis of paracetamol, guaiphenesin and chlopheniramine maleate in the
presence of phenylpropanolamine without separation step.
The calibration model is prepared by developing a series of sample
mixture comprising these drugs in certain proportion within the range 0 20
g/mL. The evaluation of calibration model was based on coefficient of
determination (R2), root mean square error of calibration (RMSEC), root mean
square error of cross validation (RMSECV), root mean square error of prediction
(RMSEP) and predicted residual sum of square (PRESS). The result showed that
Uv spectrophotometry combined with PLS can be used for quantitatve analysis of
drugs. The coefficient of determination (R2) for the relationship between actual
values and predicted values was higher than 0.99 indicating good accuracy. The
RMSEC, RMSECV, RMSEP and PRESS values obtained were relatively low
indicating good precision.
The levels of PCT, GG and CTM obtained by uv spectrophotometry in
combination with PLS are 639.38 5.23 mg/tab; 102.61 1.48 mg/tab and 1.98
0.0052 mg/tab respectively, and those by HPLC are 648.61 7.58 mg/tab; 101.07
0.80 mg/tab and 2.12 0.03 mg/tab recpectively. The precision and accuracy of
developed method was compared to that of HPLC statistically resulting not
significantly different on precision but significantly different on accuracy. The
developed method was valid and succesfully used for analysis of paracetamol,
guaiphenesin and chlorpheniramine maleate in synthetic mixture and tablet
dosage form.
Keywords: Uv-vis spectrophotometry, multivariate calibrations, multi-component
drugs.

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