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ao acaso
EAB
EQ k .p
EPQ
-3
V [ A][B ] V [P ][Q ]
K iA K mB
K mP K iQ
v
[ A] K mA [B ] K mQ [P ] [Q ] [ A][B ] K mQ [ A][P ]
1
K iA K iA K mB K mP K iQ K iQ K iA K mB K iA K mP K iQ
K mA [B ][Q ] [P ][Q ]
[ A][B ][P ]
[B ][P ][Q ]
K iA K mB K iQ K mP K iQ K iA K mB K iP K iB K mP K iQ
(51)
Relao de Haldane:
V K mP K iQ
v 0
K eq
V K iA K mB
(52)
v0
K iA K mB
V [ A][B ]
K mB [ A] K mA [B ] [ A][B ]
(53)
KiA KmB
B
A
EB
KmQ
EAB
EPQ
KmA
EP
Q
KiP
E
KmP KiQ
P
Q
EQ
V [ A][B ] V [P ][Q ]
K iA K mB
K mP K iQ
(54)
v
[ A] [B ] [P ] [Q ] [ A][B ]
[P ][Q ]
1
K iA K iB K iP K iQ K iA K mB K mP K iQ
V K mP K iQ
K eq (55)
V K iA K mB
v0
K iA K mB
V [ A][B ]
(53)
K mB [ A] K mA [B ] [ A][B ]
E
6
V [ A][B ] V [P ][Q ]
K iA K mB
K iP K mQ
v
[ A] K mA [B ] [P ] K mP [Q ] [ A][B ] [ A][P ]
K iA K iA K mB K iP K iP K mQ K iA K mB K iA K iP
K mA [B ][Q ] [P ][Q ]
K iA K mB K iQ K iP K mQ
(56)
K iA K mB K mA K iB
K iP K mQ K mP K iQ
Relao de Haldane:
V K iP K mQ
K eq (57)
V K iA K mB
V [ A][B ]
v0
K mB [ A] K mA [B ] [ A][B ]
(58)
Ex: Transaminase
5.1.1.1. Identificao de mecanismos:
1. Determinao de v0 na ausncia de produtos:
Sequencial
ordenado
Sequencial ao
acaso
Ping-pong
Mista (anticomp.)
Comp. (no
inibe)
Mista (no
inibe)
Mista (mista)
Comp. (no
inibe)
Comp. (comp.)
Comp. (comp.)
Comp. (no
inibe)
Comp. (comp.)
)Q
[ ] saturantes
do outro
B
Mistasubstrato
(no
inibe)
Comp. (no
inibe)
Mista (no
inibe)
v0
K iA K mB
V [ A][B ]
K mB [ A] K mA [B ] [ A][B ]
lim v V
[ A ]
[ B ]
= 0 (ping-pong)
Ex: [B] = cte, [A] varivel:
Vap
V [B ]
[ A]
K mB [B ]
v0
K iA K mB K mA [B ]
[ A]
K mB [B ]
Kmap
(59)
V [ A]
lim v
[ B ]
K mA [ A]
10
glucose
oxidase da
glucose
O2
H2O2
gluconolactona
peroxidase
2H2O
cromogneo pigmento
11
12
14