Absorbance at 360 nm

microliters of phosphate standard

Concentrations
(500 micromolar concentration)
Spectrometer
C term
Reading

10
41.89
0.09 48.4
0.08 60
0.07 10
15
0.06
30
0.05
60
0.04 10

0.01369Standard
0.0135 Curve
Phosphate
0.06
0.07
0.0888
0.0137
0.0207
0.0422
0.088
0.014

0.0566
0.065
0.0816y = 0.9104x + 0.0013
0.0135
0.0203
0.0405
0.0811
0.0135

0.03
0.02
0.01

0
0

0.02

0.04

0.06

0.08

0.1

Concentration (Potassium Phosphate in Water)

Samples:
N17 from 1st run
N17 - 50% glycerol
N17- 10% glycerol
N17- pooled fractions

w/o ATP w/ATP

Pi content w/o ATP Pi content w/ATP activity (amt Pi/ATP amt)
0.1505
0.9066
0.1639
0.9944 0.09944
0.167
0.8626
0.182
0.9461 0.09461
0.1957
0.6292
0.2135
0.6897 0.06897
0.1921
0.617
0.2096
0.6763 0.06763

note: 10mM ATP- I used 25 microliters of this