BIOPSY TECHNIQUES FOR

CHILDREN WITH CANCER

B.A.JABO

OUT LINE

INTRODUCTION
PREPARATION
TECHNIQUES
TYPES
CONCLUSIONS

Introduction
In the past, malignant conditions in
children were primarily diagnosed by
histopathologic techniques performed on
specimens obtained during surgery and
involved extirpation of a mass or open
biopsy.

cont
Improvements in diagnostic imaging,
newer techniques in molecular biology,
and progress n surgical techniques have
permitted accurate diagnosis and staging
of many types of childhood tumors using
minimally invasive approaches.
Simple hybridization strategies & southern
blotting were originally used.

cont
Fluorescence insitu hybridization (FISH),
polymerase chain reaction (PCR) and
reverse transcriptase.

Preparation

Consent
Haematological investigations
Biochemical
Bowel

Techniques

Open
Percutaneous
Endoscopy
Laparoscopy
Thoracoscopy

Types

Incisional
Excisional
Percutaneous (FNAC, TRUCUT)
Frozen sections

Conclusion

Important diagnosis & prognosis

Management plan
Multi displinary team work
Long term follow up

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