y = 0.014x - 0.

039
Chart Title R² = 0.970 Standard Curve Data
0.14
ng/well OD 1 OD 2 OD Avg 1/ng/well 1/OD
500.00 0.339 0.270 0.305 0.002 2.9498525
0.12 250.00 0.244 0.252 0.248 0.004 4.0983607
0.1 125.00 0.308 0.286 0.297 0.008 3.2467532
1/ng RTB per well

62.50 0.295 0.271 0.283 0.016 3.3898305

0.08 31.25 0.228 0.210 0.219 0.032 4.3859649
0.06 15.63 0.146 0.150 0.148 0.064 6.8493151
7.81 0.086 0.094 0.090 0.128 11.627907
0.04

0.02

0
0 2 4 6 8 10 12 14

1/A415

Data
SD NG
Sample 24 48 72 96 24 48 72 96
ml loaded 2 1 2 1 2 1 2 1 2 1 2 1 2 1 2 1
OD - 1 0.029 0.011 0.249 0.191 0.173 0.112 0.187 0.137 0.033 0.013 0.175 0.125 0.189 0.108 0.102 0.068
OD - 2 0.021 0.000 0.229 0.193 0.146 0.110 0.189 0.122 0.021 0.007 0.161 0.115 0.112 0.116 0.091 0.063
Avg OD 0.025 0.006 0.239 0.192 0.160 0.111 0.188 0.130 0.027 0.010 0.168 0.120 0.151 0.112 0.097 0.066
1/avg OD 40.000 181.818 4.184 5.208 6.270 9.009 5.319 7.722 37.037 100.000 5.952 8.333 6.645 8.929 10.363 15.267
1/ng per well 0.521 2.506 0.020 0.034 0.049 0.087 0.035 0.069 0.480 1.361 0.044 0.078 0.054 0.086 0.106 0.175
ng/well 1.919 0.399 51.079 29.484 20.503 11.478 28.194 14.470 2.085 0.735 22.556 12.876 18.511 11.628 9.427 5.723
ng/ml 0.960 0.399 25.540 29.484 10.251 11.478 14.097 14.470 1.043 0.735 11.278 12.876 9.255 11.628 4.714 5.723
ng in 0.5g 959.693 398.970 25539.645 29484.029 10251.301 11477.613 14097.181 14470.082 1042.713 734.754 11278.195 12875.536 9255.273 11627.907 4713.525 5722.773
Avg mg/0.5g 0.679331415 27.51183735 10.86445749 14.28363135 0.888733227 12.07686598 10.44159017 5.218149136
Avg mg/1g 0.45205221 50.92112192 21.54665136 27.55475969 0.870855834 20.05117918 20.70091671 9.423795274
 pBibKan
24 48 72 96
2 1 2 1 2 1 2 1
0.018 0.005 0.044 0.033 0.006 0.000 0.017 0.007
0.015 0.003 0.041 0.028 0.006 -0.001 0.012 0.006
0.017 0.004 0.043 0.031 0.006 -0.001 0.015 0.007
60.60606061 250 23.52941 32.786885 166.66667 -2000 68.965517 153.84615
0.809484848 3.461 0.290412 0.4200164 2.2943333 -28.039 0.9265172 2.1148462
1.235353573 0.288934 3.443387 2.3808595 0.4358565 -0.035665 1.0793107 0.4728476
0.617676787 0.288934 1.721693 2.3808595 0.2179282 -0.035665 0.5396554 0.4728476
617.6767866 288.9338 1721.693 2380.8595 217.92823 -35.66461 539.65536 472.84763
0.45330531 2.051276394 0.091131809 0.506251499
0.906610621 4.102552787 0.182263619 1.012502999

M2e SD M2e NG

60 60

50 50

40 40
24 24
48 48
mg/1g Tissue

mg/1g Tissue
72 72
30 30
96 96

20 20

10 10

0 0
 Chart Title Standard Curve Data
ng/well OD 1 OD 2 OD Avg 1/ng/well 1/OD
0.140
500.00 0.679 0.640 0.660 0.002 1.472754
y = 0.0613x - 0.0897
2 250.00 0.508 0.486 0.497 0.004 1.968504
0.120 R = 0.9306
125.00 0.660 0.578 0.619 0.008 1.515152
62.50 0.622 0.554 0.588 0.016 1.607717
0.100 31.25 0.533 0.467 0.500 0.032 1.876173
15.63 0.411 0.376 0.394 0.064 2.43309
1/ng RTB per well

0.080 7.81 0.285 0.298 0.292 0.128 3.508772

0.060

0.040

0.020

0.000
0 0.5 1 1.5 2 2.5 3 3.5 4

1/A415

Data
SD NG
Sample 24 48 72 96 24 48 72 96
ml loaded 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3
OD - 1 0.010 0.021 0.032 0.157 0.258 0.302 0.212 0.292 0.358 0.237 0.357 0.406 0.005 0.014 0.032 0.359 0.460 0.518 0.001 0.007 0.014 0.008 0.017 0.038
OD - 2 0.002 0.015 0.025 0.156 0.265 0.253 0.229 0.330 0.358 0.226 0.365 0.409 0.001 0.006 0.013 0.379 0.475 0.563 -0.003 0.001 0.008 0.006 0.018 0.043
Avg OD 0.006 0.018 0.029 0.157 0.262 0.278 0.221 0.311 0.358 0.232 0.361 0.408 0.003 0.010 0.023 0.369 0.468 0.541 -0.001 0.004 0.011 0.007 0.018 0.041
1/avg OD 166.6667 55.55556 35.08772 6.389776 3.824092 3.603604 4.535147 3.215434 2.793296 4.319654 2.770083 2.453988 333.3333 100 44.44444 2.710027 2.139037 1.850139 -1000 250 90.90909 142.8571 57.14286 24.69136
1/ng per well 10.07767 3.299889 2.051351 0.300776 0.14427 0.13082 0.187644 0.107141 0.081391 0.174499 0.079975 0.060693 20.24433 6.011 2.622111 0.076312 0.041481 0.023858 -61.089 15.161 5.456455 8.625286 3.396714 1.417173
ng/well 0.099229 0.303041 0.487484 3.324729 6.931467 7.644102 5.329241 9.333453 12.28636 5.730694 12.5039 16.4763 0.049397 0.166362 0.381372 13.10416 24.10726 41.91385 -0.01637 0.065959 0.183269 0.115938 0.294402 0.70563
ng/ml 0.099229 0.15152 0.162495 3.324729 3.465734 2.548034 5.329241 4.666727 4.095454 5.730694 6.251948 5.492099 0.049397 0.083181 0.127124 13.10416 12.05363 13.97128 -0.01637 0.032979 0.06109 0.115938 0.147201 0.23521
ng in 0.5g 99.22932 151.5203 162.4945 3324.729 3465.734 2548.034 5329.241 4666.727 4095.454 5730.694 6251.948 5492.099 49.39654 83.18084 127.124 13104.16 12053.63 13971.28 0 32.97935 61.08973 115.9382 147.2011 235.2101
Avg mg/0.5g 0.13774804 3.112832306 4.697140414 5.824913868 0.086567135 13.04302316 0.031356362 0.166116447
Avg mg/1g 0.16025335 6.151393988 9.335525127 11.35667487 0.05789154 26.0117757 0.003957023 0.039080032
 pBibKan
Sample 24 48 72 96
ml loaded 1 2 3 1 2 3 1 2 3 1 2 3
OD - 1 0.006 0.012 0.019 -0.001 0.002 0.002 0.004 0.008 0.011 0.011 0.021 0.025
OD - 2 -0.001 0.003 0.006 0.005 0.009 0.010 -0.003 0.002 0.003 0.007 0.017 0.021
Avg OD 0.003 0.008 0.013 0.002 0.006 0.006 0.001 0.005 0.007 0.009 0.019 0.023
1/avg OD 400 133.3333 80 500 181.8182 166.6667 2000 200 142.8571 111.1111 52.63158 43.47826
1/ng per well 24.311 8.044333 4.791 30.411 11.00191 10.07767 121.911 12.111 8.625286 6.688778 3.121526 2.563174
ng/well 0.041134 0.124311 0.208725 0.032883 0.090893 0.099229 0.008203 0.08257 0.115938 0.149504 0.320356 0.390141
ng/ml 0.041134 0.062156 0.069575 0.032883 0.045447 0.033076 0.008203 0.041285 0.038646 0.149504 0.160178 0.130047
ng in 0.5g 41.13364 62.15555 69.5749 32.88284 45.44666 33.07644 8.202705 41.28478 38.64606 149.5041 160.1781 130.0471
Avg mg/0.5g 0.057621365 0.037135312 0.02937785 0.146576432
Avg mg/1g 0.11524273 0.074270624 0.0587557 0.293152863

M2e SD M2e NG

30 30

25 25

20 24 20 24
48 48
mg/1g Tissue

mg/1g Tissue
72 72
15 15 96
96

10 10

5 5

0 0
 Standard OD1 OD2 Avg OD
1000 0.939 1.063 1.001
Chart Title y = 0.001x + 0.0615
2
R = 0.9793
500 0.554 0.621 0.5875 1.2
250 0.354 0.414 0.384
1
125 0.184 0.205 0.1945
62.5 0.092 0.095 0.0935 0.8
31.25 0.014 0.045 0.0295
0.6
0.4
0.2
0
0 200 400 600 800 1000 1200

Samples pBK SD NG
Hours 24 48 72 96 24 48 72 96 24 48 72
OD1 0.414 0.106 0.264 0.351 0.344 0.312 0.267 0.109 0.284 0.377 0.108
OD2 0.46 0.114 0.279 0.383 0.381 0.267 0.273 0.35 0.41 0.127
Avg OD 0.437 0.11 0.2715 0.367 0.3625 0.2895 0.27 0.109 0.317 0.3935 0.1175
mg/ml 376 49 210.5 306 301.5 228.5 209 48 256 332.5 56.5
(Dil 1/12)ug/ml 4512 588 2526 3672 3618 2742 2508 576 3072 3990 678
ng/ul 4512 588 2526 3672 3618 2742 2508 576 3072 3990 678

Sample SD
Hours 24 48 72 96
mL loaded 1 2 3 1 2 3 1 2 3 1 2
ng/ul TSP 3618 3618 3618 2742 2742 2742 2508 2508 2508 576 576
ng TSP 3618 7236 10854 2742 5484 8226 2508 5016 7524 576 1152
ug TSP/well 3.618 7.236 10.854 2.742 5.484 8.226 2.508 5.016 7.524 0.576 1.152
ng RTB/well 0.09923 0.303041 0.48748 3.324729 6.931467 7.644102 5.329241 9.333453 12.28636 5.730694 12.5039
ng RTB/ug TSP 0.027427 0.04188 0.04491 1.21252 1.263944 0.929261 2.124897 1.860736 1.632956 9.949122 10.85408
avg ngRTB/ug TSP 0.038073076 1.13524149 1.87286295 10.11269907

Sample NG
Hours 24 48 72 96
mL loaded 1 2 3 1 2 3 1 2 3 1 2
ng/ul TSP 3072 3072 3072 3990 3990 3990 678 678 678 150 150
ng TSP 3072 6144 9216 3990 7980 11970 678 1356 2034 150 300
ug TSP/well 3.072 6.144 9.216 3.99 7.98 11.97 0.678 1.356 2.034 0.15 0.3
ng RTB/well 0.049397 0.166362 0.38137 13.10416 24.10726 41.91385 0 0.065959 0.183269 0.115938 0.294402
ng RTB/ug TSP 0.01608 0.027077 0.04138 3.284251 3.02096 3.501575 0 0.048642 0.090103 0.77292 0.98134
avg ngRTB/ug TSP 0.02817947 3.268928432 0.046248361 1.107442222
 1200

96
0.086
0.061
0.0735
12.5
150
150

3
576
1728
1.728
16.4763
9.534896
10.11269907

3
150
450
0.45
0.70563
1.568067
1.107442222
# of Wells = 88 + 10 = 98 AsialoFetuin 19.60 mL 20 Sample Amnt Total Loaded Total Vol Sample in dilution Buffer D-gal in sample(mM)
Blocking Buffer 58.80 mL 60 1 100 250 2.5 247.5 10
2 100 250 5 245 10
Asialofetuin Stock 1200 mL 3 100 250 7.5 242.5 10
PBS added to stock 18.8 mL

3% BSA 1800 mg
0.01% Tween 20 6 mL

PBS Vol (L) Conc.(mM) mmol mol FW Grams in Sol'n

0.5 Dibasic 37.5 18.75 0.01875 141.96 2.66175
Monobasic 12.5 6.25 0.00625 119.98 0.749875
NaCl 150 75 0.075 58.44 4.383
D-gal conc.loaded(mM)
0.1
0.2
0.3
 3SS:TEV: Pat: M2e:RTBtr:M2e_NG

Pstl
VWWW.MH
+1 Ala Cys Arg Ser Thr Trp Trp
, . . . . . ,Ser .Thr~.»Thr His Leu Ser Thr Pro Lys
. .. . . .' lie .Ser .Lys . lie
.Gin .Ser .Gin . Lys ,Thr ..Lys Gly
.Gin---,-.
Leu Arg Leu Phe Asn Lys
... ..
1 GCCTGCAGGT
CAACATGGTGGAGCACGACA CACTTGTCTA
CTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTCAACA
+1
. ... " Tyr . Pro . Glu . Thr .Ser "Ser ,Asp. Ser ..lie Ala . Gin '..
'Lys Gly
.. Leu Ser Val Thr Leu Leu ... Arg ... Trp Lys Arg Lys Val Ala Pro Thr Asn Ala lie'
.,., . . . , . . . . . . . ,
101 AAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCA GCTATCTGTC ACTTTATTGTGAAGATAGTGGAAAAGGAAGGTGGCTCCTA CAAATGCCAT
+1 .HElie Ala lie Lys Glu Arg Pro Ser Leu Lys Met Pro Leu Pro Thr Val Val Pro Lys Met Asp Pro His Pro Arg Gly Ala Ser Trp Lys Lys Lys Thr
'" . . . . . » . ., . .. . . . . . , . . . . . , . . . . . . . ,
201 CATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTG CCGACAGTGG TCCCAAAGATGGACCCCCAC CCACGAGGAGCATCGTGGAAAAAGAAGACG
+1 Phe Gin Pro Arg Leu Gin Ser
,--, . . .. » .Lys ..Trp lie Asp Val lie Thr Trp Trp
, Ser Thr Thr His Leu Ser Thr Pro Lys lie Ser
,.,.,.»...... Lys lie Gin Ser Gin Lys Thr
301 TTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATAACATGGTGGAGCACGACACACTTGTCTACTCCAAAAATATCAAAGATACAGTCTCAGAAGA
+1 Thl Lys Leu Phe Asn Lys Gly ... Pro Glu Thr Ser Ser Ser lie Ala Gin Leu Ser Val Thr LeuLeu ... Arg ... Trp Lys
401 CCAAAGGGCAATTGAGACTTTTCAACAAAGGGTAATATCCGGAAACCTCCTCGGATTCCATTGCCCAGCT
ATCTGTCACTTTATTGTGAAGATAGTGGAA
+1 'LysArg
,...,.. Lys Val .-,..
Ala Pro Thr Asn ."Ala lie lie Ala lie Lys Glu Arg ,.Pro Ser ..,
Leu Lys,-~~..
Met Pro Leu Pro Thr Val Val Pro Lys Met Asp "'~'"
Pro His Pro
501 AAGGAAGGTGGCTCCTACAA
ATGCCATCATTGCGATAAAGGAAAGGCCATCGTTGAAGATGCCTCTGCCG
ACAGTGGTCCCAAAGATGGACCCCCACCCA
+1
. . . Ser .Trp .Lys . Lys .Lys .Thr . Phe . Gin . Pro .Arg . Leu .Gin . Ser . Lys .Trp ..lie
Arg Gly Ala Asp
. Val . lie ..Ser Pro
.Leu .Thr . ... . Gly ~
Met Thr
. His .Asn. Pro "Thr
601 CGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTC
TTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCA
+1 Thl
... lie Leu.-,..
Arg Lys Thr Leu Pro LeuTyr Lys ...
Glu Val His ...
Phe lie Trp .,..
Arg Gly Pro Arg ...
Glu Phe Ser ...
Thr Gin His lie GinAsn Lys
~~~Arg lie Ser
701 CTATCCTTCG
CAAGACCCTT
CCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACCTCGAGAATTCTCAACACAACATATACAAAACAAACGAATCTC
Kpn
w
+1 'Sel Ser Asn
~...~ Gin Ala Phe Tyr Phe Tyr Cys Ser Asn Leu Asn His PhePhe
, ... Ser Lys Ser Asn Phe Leu Lys lie Phe Thr
,..~........--. lie Tyr Glu Arg ... Pro
801 AAGCAATCAAGCATTCTACT TCTATTGCAGCAATTTAAATCATTTCTTTT AAAGCAAAAGCAATTTTCTGAAAATTTTCACCATTTACGAACGATAGCCG
Kpnl Xbal
WW\N'>IW -MNWVWVW
+1 Val Pro Met Ala Thr Thr Lys Ser Phe Leu lie LeuPhe Phe Met lie Leu Ala Thr Thr Ser Ser Thr Cys Ala Thr Ser'...,--.
Arg Ser. Leu .Leu. Thr.--,-,
GluVal
... ... ...,...
901 GTACCAATGG CAACTACTAA ATCTTTTTTA ATTTTATTTT TTATGATATT AGCAACTACT AGTTCAACAT GTGCTACGTC TAGAAGTCTT CTAACCGAGG
Nsil
wwwww
+1 Va Glu Thr Pro lie Arg Asn Glu Trp Gly Cys Arg Cys Asn Gly Ser Ser Asp Met His Pro Glu Pro lie Val Arg lie Val Gly Arg Asn Gly Leu Cys
~ , , ,
1001 TCGAAACGCCTATCAGAAACGAATGGGGGTGCAGATGCAACGGATCAAGTGATATGCATCCTGAGCCCAT
AGTGCGTATCGTAGGTCGAAATGGTCTATG
+1 'Cys Val Asp Val Arg Asp Gly Arg Phe His Asn Gly Asn Ala
.. . , .. . . . .. . .. ... . . .
. .. .,.. .~ , lie Gin Leu Trp Pro Cys Lys Ser Asn Thr Asp Ala Asn Gin Leu Trp Thr Leu Lys Arg . .
1101 TGTTGATGTTAGGGATGGAAGATTCCACAACGGAAACGCA
ATACAGTTGTGGCCATGCAAGTCTAATACAGATGCAAATCAGCTCTGGAC
TTTGAAAAGA
+1 Asp AsnThr lie ,Arg..
Ser Asn .
' Gly,Lys. Cys ..
LeuThr.Thr. Tyr . Gly..
Tyr Ser .Pro. Gly. Val ...
Tyr Val Met lie .Tyr Asp Cys'-v--r-,
Asn Thr Ala . Ala.Thr. AspAla'
. . . . ,
1201 GACAATACTATTCGATCTAATGGAAAGTGTTTAACTACTTACGGGTACAGTCCGGGAGTC TATGTGATGATCTATGATTGCAATACTGCTGCAACTGATG
Kpnl
VWWW.MH
+1 'AlaThr Arg Trp
.. . . '"Gin lie Trp Asp Asn Gly Thr . ..,lie lie Asn Pro Arg Ser Ser Leu Val Leu Ala Ala
. . . , . . . , . . Thr-, Ser . Gly , Asn . Ser . Gly~-~""-"'--'
Thr Thr Leu Thr Val
1301 CCACCCGCTG
GCAAATATGGGATAATGGAACCATCATAAATCCCAGATCTAGTCTAGTTTTAGCAGCGACATCAGGGAACAGTGGTACCACACTTACAGT

Wednesday, May 02, 20073:16:37 PM Page 1

.
 355: TEV: Pat: M2e: RTBtr: M 2e _N G

+1 Va
,. Gin. Thr . Asn ...lie Tyr Ala .. Val . Ser . Gin. Gly ..Trp Leu Pro ..Thr ,Asn..,
Asn Thr . Gin. Pro , Phe . Val ..Thr , Thr lie Val Gly . Leu ..Tyr Gly ..Leu .Cys..Leu. Gin
1401 GCAAACCAACAmATGCCG TTAGTCAAGGTTGGCTTCCTACTAATAATACACAACcm TGTGACAACCATTGTTGGGCTATATGGTCTGTGCTTGCAA
+1 Ala Asn Ser Gly Gin Val Trp lie Glu Asp Cys Ser Ser Glu Lys ..
Ala Glu ..
Gin Gin ..Trp ...".
Ala Leu Tyr Ala .Asp..
Gly . Ser . lie . Arg.. Pro .'~Gin Gin Asn Arg'
, .. .. .. ..
1501 GCAAATAGTGGACAAGTATGGATAGAGGACTGTAGCAGTGAAAAGGCTGAACAACAGTGGGCTCmATG CAGATGGTTCAATACGTCCTCAGCAAAACC
+1 'ArgAspAsn Cys Leu Thr Ser Asp Ser Asn lie Arg Glu Thr Val Val Lys lie LeuSer Cys Gly Pro Ala Ser Ser Gly Gin Arg Trp Met Phe LysAsn'
, . ... .. . .. . . . . . . .. . . .. ... .. . , .. . ,
1601 GAGATAATTGCCTTACAAGTGATTCTAATATACGGGAAACAGTTGTCAAGATCCTCTCTTGTGGCCCTGC ATCCTCTGGCCAACGATGGATGTTCAAGAA
'AsnAsp Gly Thr lie LeuAsn Leu Tyr Ser Gly Leu Val Leu Asp Val Arg Ala Ser Asp Pro Ser Leu Lys Gin lie lie Leu Tyr Pro Leu His Gly Asp
+1
..., ... ...~-...
1701 TGATGGAACC ATTTTAAATT TGTATAGTGG GTTGGTGTTA GATGTGAGGG CATCGGATCC GAGCCTTAAA CAAATCATTC mACCCTCT CCATGGTGAC
Ndel
vwwwwvw
+1 Pro Asn Gin lie Trp Leu Pro LeuPhe His Met Ser Leu Leu Thr Glu Val Glu Thr Pro
. .. ~ .. .. .. . . . , . . . lie ..Arg ..Asn Glu Trp Gly Cys Arg Cys Asn Gly Ser Ser Asp'
. . . . . . "'W.~ "..,
1801 CCAAACCAAATATGGTTACCATTAmCAT ATGAGTCTTCTAACCGAGGTCGAAACGCCT
ATCAGAAACGAATGGGGGTGCAGATGCAACGGATCAAGTG
Sacl
WW>IWW>N
+1
. .
'Asp Glu Leu
1901 ATGAGCTC

Wednesday, May 02,20073:16:37 PM Page 2 "

.
 35S:TEV: Pat: M2e: RTBtr: M2e_NG

XbaI (980) NdeI (1830)

Patatin M2e.5' KpnI (1389) M2e.3'
I RTBtr I Sac! (1908)
Pst! (8\ 35S::TEV Kpnl (9°5) \ I NsiI (1059) I

355 :TEV:Pat:M2e:RTBtr:M2e NG
1908 bp

Wednesday, May 02, 2007 3:15:45 PM Page 1

>Pat:RTB:M2e_SD

GGTACCAATGGCAACTACTAAATCTTTTTTAATTTTATTTTTTATGATATTAGCAACTACTAGTTCAACATG
TGCTACGTCTAGAGCTGATGTTTGTATGGATCCTGAGCCCATAGTGCGTATCGTAGGTCGAAATGGTCTA
TGTGTTGATGTTAGGGATGGAAGATTCCACAACGGAAACGCAATACAGTTGTGGCCATGCAAGTCTAAT
ACAGATGCAAATCAGCTCTGGACTTTGAAAAGAGACAATACTATTCGATCTAATGGAAAGTGTTTAACTA
CTTACGGGTACAGTCCGGGAGTCTATGTGATGATCTATGATTGCAATACTGCTGCAACTGATGCCACCCG
CTGGCAAATATGGGATAATGGAACCATCATAAATCCCAGATCTAGTCTAGTTTTAGCAGCGACATCAGGG
AACAGTGGTACCACACTTACAGTGCAAACCAACATTTATGCCGTTAGTCAAGGTTGGCTTCCTACTAATAA
TACACAACCTTTTGTGACAACCATTGTTGGGCTATATGGTCTGTGCTTGCAAGCAAATAGTGGACAAGTA
TGGATAGAGGACTGTAGCAGTGAAAAGGCTGAACAACAGTGGGCTCTTTATGCAGATGGTTCAATACGT
CCTCAGCAAAACCGAGATAATTGCCTTACAAGTGATTCTAATATACGGGAAACAGTTGTCAAGATCCTCT
CTTGTGGCCCTGCATCCTCTGGCCAACGATGGATGTTCAAGAATGATGGAACCATTTTAAATTTGTATAGT
GGGTTGGTGTTAGATGTGAGGGCATCGGATCCGAGCCTTAAACAAATCATTCTTTACCCTCTCCATGGTG
ACCCAAACCAAATATTGTTACCATTATTTCTCGAGAGTCTTCTAACCGAGGTCGAAACGCCTATCAGAAAC
GAATGGGGGTGCAGATGCTCTGATTCAAGTGATGAGCTC

>Pat:RTB:M2e_NG

GGTACCAATGGCAACTACTAAATCTTTTTTAATTTTATTTTTTATGATATTAGCAACTACTAGTTCAACATG
TGCTACGTCTAGAGCTGATGTTTGTATGGATCCTGAGCCCATAGTGCGTATCGTAGGTCGAAATGGTCTA
TGTGTTGATGTTAGGGATGGAAGATTCCACAACGGAAACGCAATACAGTTGTGGCCATGCAAGTCTAAT
ACAGATGCAAATCAGCTCTGGACTTTGAAAAGAGACAATACTATTCGATCTAATGGAAAGTGTTTAACTA
CTTACGGGTACAGTCCGGGAGTCTATGTGATGATCTATGATTGCAATACTGCTGCAACTGATGCCACCCG
CTGGCAAATATGGGATAATGGAACCATCATAAATCCCAGATCTAGTCTAGTTTTAGCAGCGACATCAGGG
AACAGTGGTACCACACTTACAGTGCAAACCAACATTTATGCCGTTAGTCAAGGTTGGCTTCCTACTAATAA
TACACAACCTTTTGTGACAACCATTGTTGGGCTATATGGTCTGTGCTTGCAAGCAAATAGTGGACAAGTA
TGGATAGAGGACTGTAGCAGTGAAAAGGCTGAACAACAGTGGGCTCTTTATGCAGATGGTTCAATACGT
CCTCAGCAAAACCGAGATAATTGCCTTACAAGTGATTCTAATATACGGGAAACAGTTGTCAAGATCCTCT
CTTGTGGCCCTGCATCCTCTGGCCAACGATGGATGTTCAAGAATGATGGAACCATTTTAAATTTGTATAGT
GGGTTGGTGTTAGATGTGAGGGCATCGGATCCGAGCCTTAAACAAATCATTCTTTACCCTCTCCATGGTG
ACCCAAACCAAATATTGTTACCATTATTTCTCGAGAGTCTTCTAACCGAGGTCGAAACGCCTATCAGAAAC
GAATGGGGGTGCAGATGCAACGGATCAAGTGATGAGCTC