Instruction

Manual:
How to Use a

Name___________________
Date_________________
Division___________
Practice Notes:

(As you Practice, take notes)

Label the Parts of the Light

Microscope Parts:
Microscope
Eyepiece
Diaphragm
Stage
Base
Arm
Light Source Stage Clips
Body Tube
Nosepiece
Fine Adjustment Knob
Coarse Adjustment Knob
Objective Lenses

Microscope Instructions
1. Plug in the microscope and turn on the lamp.
2. Place the Low Power Objective Lens (the
shortest) facing towards the stage
3. Place the slide on the stage and secure in place
with stage clips.
4. Use the Coarse Adjustment Knob (large knob)
to bring the slide into view then use the Fine
Adjustment Knob (small knob) to make it clearer.
5. Once you have the image in view, rotate the
nosepiece to view it under different magnifications.
**Be careful if you want to use the highest power objective lens (the long
one). If the stage is too close it might not fit and therefore scratch the lens!
**

6. When done:
turn off the lamp
place low power
objective facing
down
put the slide away
wrap the cord
cover the
microscope

Fill in Notes

Microscope Instructions
1. Plug in the microscope and turn on the
______________.
2. Place the ________________________________________
(the shortest) facing towards the stage
3. Place the ________________ on the _______________
and secure in place with ______________.
4. Use the ___________ adjustment knob (large
knob) to bring the slide into view then use the
____________ adjustment knob(small knob) to make
it clearer.
5. Once you have the image in view, rotate the
_____________ to view it under different
magnifications.
**Be careful if you want to use the highest power
objective lens (the __________ one). If the stage is too
_________ it might not fit and therefore _____________ the
lens!**

6. When done:

 Turn off the __________

Place low power
objective facing
________
Put the _________away
Wrap the ____________
________ the microscope

How to Carry a
Microscope:
1. Make sure the cord is wrapped around the arm (and
the end is tucked in) and that the low power
objective is facing down.
2. Place one hand on the arm,
and the other hand on the
base.
3.Lift up and walk carefully.

How to Calculate the Total

Magnification:

Multiply the power of the eyepiece lens by the power

of the objective lens. Our microscopes have an
eyepiece lens magnification of 10x.
Low Power:
10x (eyepiece lens) x 4x (low power objective)
= 40 times magnification
Medium Power:
10x (eyepiece lens) x 10x (medium power objective)
= 100 times magnification
High Power:
10x (eyepiece lens) x 40x (high power objective)
= 400 times magnification
Fill in Notes

Eyepiece
(10x)

Objective
Lenses:
Low (4x)
Medium(10x)
High (40x)

How to Carry a
Microscope:
1. Make sure the ___________ is
wrapped around the arm (and
the end is tucked in) and that
the ________________________ is
facing down.
2. Place one hand on the ______, and the other hand
on the ____________.
3. Lift up and walk ___________________.

How to Calculate the Total

Magnification:
Multiply the power of the ______________ lens by the power of
the ________________ lens. Our microscopes have an eyepiece lens
magnification of ______x.
Eyepiece

Low Power:
(10x)
10x (eyepiece lens) x 4x (low power objective)
= __________times magnification
Medium Power:
10x (eyepiece lens) x 10x (Medium power objective)
= ____________ times magnification
High Power:
Objective
10x (eyepiece lens) x 40x (High power objective)
Lenses:
Low (4x)
= ____________ times magnification

Microscope Stations:
Prepared Slides
Name________________________________________________
Date_________________ Division ___________
Directions: Observe the slides at each station. Draw what you see
through the eyepiece. Record your observations.

Medium(10x)
High (40x)

Total Magnification:

10 (eyepiece lens)
x _____ (objective lens)
= ______ magnification

Specimen
Name:_________________
Description:
Total Magnification:

10 (eyepiece lens)
x _____ (objective lens)
= ______ magnification

Specimen
Name:_________________
Description:

Total Magnification:

10 (eyepiece lens)
x _____ (objective lens)
= ______ magnification

Specimen
Name:_________________
Description:
Total Magnification:

10 (eyepiece lens)
x _____ (objective lens)
= ______ magnification

Specimen
Name:_________________
Description:

Analysis: Describe any thoughts or questions that

came up while you were observing these slides.

______________________________________________________
______________________________________________________
______________________________________________________

Practice Notes:

(As you Practice, take notes)

Microscope TEST:

Microscope License:

Earned Needs More

Practice

Name: ________________________________________

Focusing Steps:

Points Possible

1.Uncover microscope, place cover by

wall, Plug in the microscope THEN turn
on the lamp.
2. Place (or make sure) the Low Power
Objective Lens (the shortest) facing
towards the stage
3. Place the slide on the stage and
secure in place with stage clips.

10pt
s
5pts

5 pts if some
parts are done
correctly

10pt
s
10pt
s
10pt
s
10pt
s
10pt
s
10pt
s

5 pts if some
parts are done
correctly

0 pts if none or
more than half
are not done

5 pts if fine
adjustment
knob is used
only
5pts if fuzzy but
can see the
specimen

0 pts if no
focusing is done

5 pts if some
parts are done
correctly

0 pts if none or
more than half
are not done

5pts if fuzzy but

can see the
specimen

0 pts if no
specimen is
visible

5pts if some
parts are done
correctly

9. Check to see if it is in focus

10pt
s

5pts if fuzzy but

can see the
specimen

0 pts IF COARSE
ADJUSTMENT
KNOB IS
USED!!!! Or if
no specimen is
visible
0 pts if no
specimen is
visible

10. When done:

These steps must be done in order:

4. Use the Coarse Adjustment Knob

(large knob) to bring the slide into view
5. Check to see if it is in focus (low
power)
6. Rotate the nosepiece, Focus on
Medium power using Coarse
Adjustment Knob (large knob)
7. Check to see if it is in focus (medium
power)
8. Rotate the nosepiece, Focus on High
power ONLY using the Fine Adjustment
Knob (small knob) to make it clearer.

turn off the lamp

place low power objective

facing down

3pts
3pts

0 pts if none or
more than half
are not done

0 pts if no
checking is
done

0 pts if not
done
0 pts if not
done

0 pts if no
specimen is
visible

Points
Earne
d

put the slide away

unplug and wrap the cord

cover the microscope

Total Points Earned:

3pts
3pts
3pts
100
pts

0 pts if not
done
0 pts if not
done
0 pts if not
done

Microscope Test:
Name: _________________________________ Date: ______
Microscope Parts:
Eyepiece
Diaphragm Base Light Source
Revolving Nosepiece Coarse
Adjustment Knob
Arm
Stage Clips
Stage
Body Tube Objective Lenses Fine

Part 1: Identify the Parts

of the Microscope
(2 pts each - Spelling Counts)

A. _____________________
B. _____________________
C. _____________________
D._____________________
E. _____________________
F. _____________________
G._____________________
H._____________________
I. _____________________
Part
2: Identify the Functions of the Parts of the Microscope
J. _____________________
(2
pts each)
K. _____________________
Use
the letters of the parts above. Write the letters on the lines to the
L. _____________________
left of the
______1.
______2.
______3.
______4.
______5.
______6.
______7.
______8.
______9.

function.
Moves the stage up and down for focusing
Supports the microscope slide
Supports the body tube and is used to carry the microscope
Contains the lens you look through
Provides the magnification (4x, 10x, and 40x)
Supports the microscope on the table
Holds the microscope slides in place
Controls the amount of light that enters the body tube
Moves slightly and is used to sharpen the image if the object
you are viewing

Part 3: List the 3 Important Steps for Carrying the

Microscope (10 pts each)
1. __________________________________________________________________
2. __________________________________________________________________
3. __________________________________________________________________
Part 4: When Done ( 2 pts each)
Put these steps in the correct order by numbering them (from 1 to 5)
on the line to the left.

______. Cover the microscope

______.Turn off the lamp
______.Place low power objective facing down
______.Put the slide away
______. Unplug and Wrap the cord
Part 5: Important Things to Remember (2 pts each)
1. Where are you supposed to plug in the microscope?
_________________________________
2. Why do you plug it in there?
____________________________________________________
3. What do you check if the microscope doesnt seem to turn on?
_____________________________________________________________________________
4. Which Adjustment Knob do you use with the high power lens?

Part 6: Magnification (2 pts each)

1. If you are looking at something on the low power objective (4x), how
many times bigger will it appear? _____________
2. If you are looking at something on the medium power objective (10x),
how many times bigger will it appear? _____________
3. If you are looking at something on the high power objective (40x), how
many times bigger will it appear? _____________

Written Test Score:

Practical Microscope Test Score:

________ / 100
________ / 100