Name Aa Pa. koa Exam IV (100 points) CEM 333 December 3, 2014 1.(5 pts.) What kind of information can one obtain from IR spectroscopy and what portion of the electromagnetic spectrum (in wavelength) is typically used for measurements? rfermetion about (eketins aud ubrthinn vi tubeuls (Mo Shuctay a be datirrne ; frcernclans gp derulls,hy — AS PL Me Ste eucl 2.(Spts) If the average molar extinction coefficient for a typical IR absorption band is 10 L/mole-cm and absorbance changes as low as 10“ AU can be measured, then what is the predicted minimum concentration detectable. Assume a transmission measurement with a path length of 250 ym. -t aie e Jato Mem, fos C=10 Lem — b= Bum ATO ey “i Az lo's 2bc és /e . 4 : cant dy ‘bm (18 ycrens) Gem is) a 3. (15 pts) Sketch the design of a typical fourier transform IR spectrometer. Label all key parts and show the optical path. _ eae eee faecrrig T ! Te Sues A.----- fae Al teulelecunck Heda \ duulbausously , redulaitid Kreal daticke A Chuarleeirtee feapiue by © Hf the Weriy Mure 4 fay4. (10 pts) _ Indicate four major advantages of the FT-IR instruments compared to their dispersive instrument counterparts. 1. Hae apheal theewfegat 2. Hah apud 3 High jeceliitin ; : 4. A crude fin prmed suablestusly ~ deg! eevecagein te dageree Wi 5. (10 pts) Assume you were hired by the EPA to address Pa a contamination problem in Kalamazoo. The pesticide, atrazine (FW=216 g/mol, bp. = 200 °C), has contaminated N soil in a rail yard. It is likely the pesticide will migrate into the ae underground water table. Propose a relatively detailed N N method for determining what the mass of atrazine is per kg of soil at the site, Assume you selected IR for identifying the presence of the pesticide. What peaks would you be looking for in the spectrum? Be specific for credit + Tele dongles Low agud palliun aesun Ha stb laybe alae crux sue ph tgn ao cell + Streut orkut weld Ce ud G Leune abegu fom Sot, D pusen pun 8 det arwll Hn Ce tyered 2, tune vetu ben enjaue Silueut [ey trteuu). A gawittobeltae $ofacFo laotd Ce Weed’ HM tecndd thw be coud sttteleen, Ttaudad addiction corse foc ” ee Doisnahalaies ot es PEELS by Capt Live agrttotortan ¥ Ky Cour ex. Lie Apativin aeullle$ C-Cl b00-4oras' Nel Geenercrsinn) ~ Seen! alge“ B8to- Foon WH bead "(Gtim) ~ (920 -Lececut 6.(10 pts) Sketch the basic design of a mass spectrometer (a block diagram would be sufficient) and label all key components. e * te ae i = ; \ Femaativ Mise Ton : Pe | Ree |e | 27. (10 pts) Assume you could take a soil sample, place it on a heated probe and insert the probe directly into the mass spectrometer. Furthermore, assume you are able to perform a chemical ionization of the desorbed/vaporized atrazine. Describe how the chemical ionization process works. What m/z would you be looking for in the mass spectrum if atrazine is present? Melheasin dex wed fr Heo rungere Cy +o 3 Chyt + dem Guy +t —= CHS + Cs Chy + M > CHa + mut One main one Weld Le Leobey fz decal be [-HIT Hur ELAM isAPOAVMA, Mh= 317 Gerd 8. (10 pts) Assume you are able to perform electron impact ionization. How does El work and what kind of information does it provide that can be used to identify the molecule? We did not cover fragmentation in any detail but generally speaking halogen groups are easily cleaved. With this information, what three m/z peaks would you be looking for in the El mass spectrum? € Cees form Aw .eue absilion Cecten Sef: inye fan i =e ° ss = Beuas Lin. eh C5 ee ey hea apt pai ht had dome font dulegges Pergpussifaten 7 b tuontd be: . a, te 2) fle pretriite —€1 hea Zle-35 = (3) 9. (5 pts) What are the two general types of electroanalytical measurements? Pedewticnate, §— Epeawuud X celle Criteabse ox aclunty Voj tauictcca Lpuay 6 Autclifte Criterion 20 BI