CHAPTER 9 ENDANGERED ECOSYSTEM ( Water Pollution )

Level of Water Pollution in Different Sources of Water

Aim/
Objective

: To analyze the level of water pollution in different source of water/

To investigate the level of pollution in several different source of water

Problem

: What is the level of water pollution in different source of water?

Hypothesis

Water sample P more polluted /the quicker the time taken for the methylene
blue solution to turn colourless than water sample Q,R and S.

Variables
Manipulated : Different sources of water
Responding : Time taken for methylene blue solution to decolorize
Fixed
: Volume of methylene blue solution , Volume of water sample
Material
Apparatus

: 0.1 % methylene blue solution and water samples

: Three reagent bottles ( depends on the number of water samples) and
syringe, stop watch,measuring sylinder,stopper,

Technique

: The time for the methylene blue solution to decolorize is taken using a
stopped watch

Procedure

: 1. Three (more )samples of water from different sources were collected

2. Each labeled reagent bottle was filled with 200 ml of the collected water
samples respectively
3. Using a syringe, 1 ml of 0.1 % methylene blue solution was added to the
base of water sample slowly.
4. Immediately, the reagent bottle was closed with a stopper.
5. Step 2 to 4 were repeated using distilled water as a control.
6. All the bottles were placed in dark area ( cupboard)
7. The change in the colour of the sample was check every one hour.
8. The time taken for the methylene blue solution to decolorize was recorded
in a table.
:
Water sample
Time taken for methylene blue solution to
decolorised (min)
P
10
Q
35
R
60
S
42

Result

Conclusion

Hypothesis is accepted. The water sample which contains the least oxygen
content is the most polluted.

Note
1. If the oxygen content in the water is low, the time
taken for methylene blue solution to decolorise is
shorter
2. The number of microorganism high, because they use the oxygen .