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동물세포 배양

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Subculture

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Subculture

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30 views1 page

동물세포 배양

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Subculture

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(Subculture)

1.
Materials : hMSC(E9), PBS, trypsin-EDTA, -MEM
Procedure
1. disposable pipette
2. PBS 5mL 2 ,
3. Trypsin-EDTA 500L CO2 2
4. Trypsin inhibitor 10mL 15mL conical tube
5. 1.7mL EP tube 1mL
6. 6 EP tube 1000rpm 2 centrifuge
7. EP tube pellet
8. -MEM 1mL pipette suspension
9. hemacytometer
10. EP tube T-flask CO2

2.
Cell counting 3.08*10^5 /mL

3. Discussion

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