Tutorial: Enzymes

Question 1
Explain the following:
1.1)
1.2)
1.3)
1.4)
1.5)

Catalyst
Substrate
Active site
How enzymes differ from chemical catalysts?
Stereospecificity

Question 2
2.1) List and discuss the six classes of enzymes.
2.2) a process that occurs in the human body is shown in the diagram. What would
happen if a temperature change caused the shape of the active site to be altered?

2.3) Enzymes have an optimum temperature at which they work best. Temperatures
above and below this optimum will decrease enzyme activity. Explain which graph best
illustrates the effect of temperature on enzyme activity?

Question 3
Answer True or False
3.1) Lineweaver-Burk plots describe the equation of a rectangular hyperbolic curve?
3.2) all monosaccharides are reducing sugars.
3.3) the enzyme active site binds the substrate with higher affinity than the transition
state.
3.4) the enzyme active site binds the substrate with higher affinity than the transition
state.
3.5) under physiological conditions, the substrate concentration is < to the Km.

Question 4
4.1) for an enzyme that follows Michaelis-Menton kinetics, Km is equal
to___________?
4.2) what occurs when an enzyme that follows Michaelis-Menten kinetics is treated
with a competitive inhibitor?
4.3) what occurs when an enzyme that follows Michaelis-Menten kinetics is treated
with a noncompetitive inhibitor?

Question 5

5.1) What class of enzyme catalyzes the following reaction?

5.2) what are the three kinds of reversible inhibitors? Illustrate using Lineweaver Burk
plots how you can distinguish them. Draw a diagram to show how these inhibitors act
on the enzyme.

Question 6
The kinetic data from an enzyme catalysed reaction performed with and without
inhibitor is given below. From this information determine the Kms and Vmaxs for the
enzyme with and without inhibitor by plotting the data on a Lineweaver Burk (double
reciprocal) plot. Label X-axis and Y-axis with correct title and units. Determine the type
of inhibition that is occurring in this experiment.

Use the below graph paper.