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TR0068 Protein Assay Compatibility
TR0068 Protein Assay Compatibility
Introduction
No single protein assay method is compatible with all sample components that might be encountered in typical laboratory
research. To alleviate the frustration of discovering these incompatibilities by trial and error, our technical support scientists
have characterized the effects of commonly used buffers and sample components on the behavior of seven different Thermo
Scientific Pierce* Protein Assays.
Results of these compatibility experiments are summarized in tabular form on the following two pages. The table serves as a
guide for assessing which protein assay(s) might be most effective with a given sample type. Use this information as a guide
only. It is the researchers responsibility to validate the compatibility of specific sample buffers. Even minor changes in
buffers and components affect each assay method to some extent. Researchers must decide if these effects are acceptable for
their specific purposes.
Part No.
Name
22662
660 nm
23225
BCA
23235
Micro BCA
23250
23252
Microplate BCA-RAC
23240
Modified Lowry
23236
23200
Coomassie (Bradford)
Compounds are listed alphabetically using common names or abbreviations, except sodium compounds which are alphabetized under Na.
Dilutions are expressed as neet (= undiluted) or in the form of a ratio, where 1:2 means 2-fold dilution.
n/a Denotes that the compound was not tested in this assay.
Denotes compounds that were not compatible at the lowest concentration tested.
* Thermo Scientific product trademark; PER Reagents are Thermo Scientific Pierce Cell Lysis Reagents.
** Trademarks that are the property of other companies; see end of document.
Value when the 660nm Assay is run using the ionic detergent compatibility reagent (IDCR, Part No. 22663).
Selected values for the regular BCA-RAC Kit are given in parentheses in the column for the Microplate BCA-RAC.
Compound (buffer) whose formulation is described more fully in the following table:
Part No.
28390
28374
28382
28388
28386
28372
89900
28379
28380
28378
28398
Buffer
Formulation
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660 nm
BCA
Micro BCA
Microplate
BCA-RAC
Coomassie
Plus
Coomassie
Modified
Lowry
neet
1M
50mM
50%
50%
125mM
2mM
500mM
40mM
>1M
50mM
neet
1:2
1:2
1:2
5%
n/a
5%
0.031%
40mM
100mM
2.5%
5%
4%
>500mM
20mM
2.5%
350mM
25mM
500mM
50%
50%
2%
20mM
20mM
200mM
50%
5mM
500mM
100mM
50%
100mM
2.5M
100mM
125mM
200mM
1:4
neet
80M
100mM
500mM
neet
neet
125mM
50%
>1M
neet
125mM
1:2
n/a
0.01%
25mM
10%
10%
1.5M
10mg/L
1mM
20mM
33mM
neet
neet
n/a
n/a
5%
1%
1%
10mM
100mM
n/a
5%
5%
100mM
0.8mM
n/a
1mM
1mM
10%
10%
n/a
10mM
100mM
10%
10mM
10mM
n/a
10%
100mM
4M
100mM
100mM
50mM
neet
10mg/L
n/a
neet
neet
100mM
10%
n/a
neet
100mM
neet
n/a
1mM
10mM
1%
1%
1mg/L
n/a
2mM
0.2mM
1:4
n/a
n/a
n/a
5%
1%
1%
10mM
100mM
n/a
1%
5%
100mM
n/a
1%
1%
n/a
0.5mM
100mM
1%
0.5mM
1mM
n/a
1%
n/a
4M
100mM
10mM
12.5mM
n/a
10mg/L
n/a
n/a
neet
1:4
100mM
1%
n/a
neet
100mM
n/a
n/a
25mM (35)
30%
n/a
1mM
16.5mM
1:3
1:4
1:4
0.63%
n/a
0.50%
1mM
n/a
10% (10)
50mM
0.4mM
n/a
2.5mM
2.5mM
5mM (5)
5%
0.25%
n/a
5mM (20)
5mM (10)
5mM
10mM
5%
50mM
1.5M (2)
200mM (200)
n/a
30mM (50)
n/a
n/a
n/a
1:2
100mM (100)
0.5%
100mM
neet
200mM
1:2
n/a
1M
100mM
10%
10%
1M
10mg/L
50mM
10mM
100mM
100mM
neet
1:2
1:4
n/a
0.062%
0.031%
0.016%
10mM
100mM
n/a
5%
5%
100mM
10mM
n/a
10mM
1mM
5mM
10%
10%
n/a
100mM
2mM
100mM
10%
10mM
1M
n/a
10%
100mM
3.5M
100mM
n/a
100mM
200mM
n/a
10mg/L
n/a
100mM
neet
neet
100mM
10%
n/a
neet
100mM
neet
n/a
1M
100mM
10%
10%
1M
10mg/L
50mM
10mM
100mM
100mM
neet
1:2
n/a
n/a
0.125%
0.031%
0.031%
10mM
100mM
n/a
5%
5%
100mM
10mM
n/a
10mM
1mM
5mM
10%
10%
n/a
100mM
2mM
100mM
10%
10mM
1M
n/a
10%
100mM
3.5M
100mM
n/a
100mM
200mM
n/a
10mg/L
n/a
100mM
n/a
neet
100mM
10%
n/a
neet
100mM
n/a
n/a
1mM
n/a
10%
10%
10mg/L
1mM
5mM
n/a
n/a
n/a
n/a
n/a
n/a
0.031%
0.062%
0.062%
n/a
50mM
n/a
0.062%
0.031%
n/a
n/a
n/a
1mM
10%
10%
n/a
1mM
1mM
n/a
10%
n/a
100mM
n/a
10%
100mM
n/a
1mM
n/a
100mM
25mM
n/a
10mg/L
n/a
25mM
n/a
n/a
125mM
10 %
n/a
n/a
n/a
n/a
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Continued
Test Compound
n-Acetylglucosamine
Na acetate pH 4.8
Na azide
Na bicarbonate
Na carb-bicarbonate pH 9.4
Na chloride
Na citrate pH 4.8
Na citrate-carbonate pH 9
Na citrate-MOPS pH 7.5
Na deoxycholate (DOC)
Na hydroxide (NaOH)
Na phosphate
NE-PER* Reagent (CER)
NE-PER Reagent (NER)
Nickel chloride (in TBS pH 7.2)
NP-40
Octyl beta-glucoside
Octylthioglucoside
Na-orthovanadate (in PBS pH 7.2)
Phenol Red
Phosphate-buffered saline (PBS)
PIPES pH 6.8
PMSF in isopropanol
Potassium thiocyanate
P-PER Reagent
RIPA buffer
SDS
Sodium compounds
Span** 20
Sucrose
TCEP
Thimerosal
Thiourea
TLCK
TPCK
T-PER* Reagent
Tricine pH 8.0
Triethanolamine pH 7.8
Tris-buffered saline (TBS)
Tris-glycine pH 8.0
Tris-glycine-SDS pH 8.3
Tris-HCl pH 8.0
Tris-HEPES-SDS
Triton** X-100
Triton X-114
Triton X-305
Triton X-405
Tween** 20
Tween 60
Tween 80
Urea
Y-PER* Reagent
Y-PER Plus Reagent
Zinc chloride (in TBS pH 7.2)
Zwittergent** 3-14
660 nm
100mM
100mM
0.125%
100mM
1:3
1.25M
12.5mM
1:16
0.25%
125mM
500mM
neet
neet
10mM
5%
5%
10%
50mM
0.5mg/mL
neet
100mM
1mM
250mM
1:2
neet
0.01%, 5%
(see Na)
n/a
50%
40mM
0.25%
2M
5mg/mL
4mg/mL
1:2
500mM
100mM
neet
neet
neet
250mM
neet
1%
0.50%
9%
5%
10%
5%
5%
8M
1:2
10mM
0.05%
BCA
10mM
200mM
0.2%
100mM
neet
1M
200mM
1:8
1:8
5%
100mM
100mM
neet
neet
10mM
5%
5%
5%
1mM
neet
100mM
1mM
3M
neet
5%
(see Na)
1%
40%
0.01%
n/a
0.1mg/L
0.1mg/L
1:2
25mM
25mM
neet
1:3
neet
250mM
n/a
5%
1%
1%
1%
5%
5%
5%
3M
neet
neet
10mM
1%
Micro BCA
200mM
0.20%
100mM
neet
1M
5mM
1:600
1:600
5%
50mM
100mM
n/a
n/a
0.2mM
5%
0.1%
5%
1mM
neet
100mM
1mM
n/a
n/a
1:10
5%
(see Na)
1%
4%
n/a
0.1mg/L
0.1mg/L
n/a
2.5mM
0.5mM
1:10
1:10
neet
50mM
n/a
5%
0.05%
1%
1%
5%
0.5%
5%
3M
n/a
n/a
0.5mM
Microplate
BCA-RAC
0.01%
neet
150mM
50mM
neet
n/a
100mM
1:2
1:4
2.5% (10)
7%
0.5mM
3.125g/mL
neet
25mM
0.125mM
1:2
1:2
5% (10)
(see Na)
n/a
40% (40)
10mM (10)
0.03%
n/a
n/a
0.5mM
25mM
neet
35mM (50)
n/a
7% (10)
2% (2)
1%
10% (10)
5%
2.5%
3M (4)
n/a
n/a
2% (2)
Coomassie
Plus
100mM
180mM
0.5%
100mM
neet
5M
200mM
neet
n/a
0.4%
100mM
100mM
1:4
neet
10mM
0.5%
0.5%
3%
1mM
0.5mg/mL
neet
100mM
1mM
3M
1:40
0.016%
(see Na)
0.5%
10%
125mM
0.01%
n/a
0.1mg/mL
0.1mg/mL
neet
100mM
100mM
neet
neet
1:4
2M
n/a
0.062%
0.062%
0.125%
0.025%
0.031%
0.025%
0.016%
3M
n/a
neet
10mM
0.025%
Coomassie
100mM
180mM
0.5%
100mM
neet
5M
200mM
neet
neet
0.05%
100mM
100mM
n/a
n/a
10mM
0.5%
0.5%
3%
1mM
0.5mg/mL
neet
100mM
1mM
3M
1:10
0.125%
(see Na)
0.5%
10%
125mM
0.01%
n/a
0.1mg/L
0.1mg/L
n/a
100mM
100mM
neet
neet
1:2
2M
n/a
0.125%
0.125%
0.5%
0.5%
0.062%
0.1%
0.062%
3M
n/a
n/a
10mM
0.025%
Modified
Lowry
n/a
200mM
0.2%
100mM
n/a
1M
n/a
n/a
n/a
n/a
100mM
100mM
n/a
n/a
n/a
0.016%
0.031%
n/a
n/a
n/a
n/a
n/a
1mM
100mM
n/a
1%
(see Na)
0.25%
7.5%
n/a
0.01%
n/a
0.01mg/L
0.1mg/L
n/a
n/a
n/a
n/a
n/a
n/a
10mM
n/a
0.031%
0.031%
0.031%
0.031%
0.062%
n/a
0.031%
3M
n/a
n/a
n/a
n/a
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