Electronic Journal of Biotechnology 17 (2014) 14 18

Production and characterization of algae extract from Chlamydomonas Reinhardtii

Weston Kightlinger, Kai Chen, Azadeh Pourmir, Daniel W. Crunkleton, Geoffrey L. Price, Tyler W. Johannes
Department of Chemical Engineering, The University of Tulsa, 800 South Tucker Drive, Tulsa, OK 74104,
USA

Presented by :
Irwansyah Putra

Post Graduate Program Andalas University

Effects of algae extract on bacterial metabolism

Aerobic growth of E. coli on
LB medium containing 5 g/L
of either yeast extract (YE)
or algae extract (AE). Each
measurement represents the
mean of triplicate
experiments standard
deviations.

Specific growth rates and protein production from E. coli grown on LB medium containing 5 g/L of either yeast
extract (YE) or algae extract (AE). Each measurement represents the mean of triplicate experiments standard
deviations.

Effects of algae extract on yeast metabolism

Anaerobic growth
of S. cerevisiae on
YPAD medium
containing 10 g/L
of either yeast
extract (YE) or
algae extract
(AE). Each
measurement
represents the
mean of triplicate
experiments sta
ndard deviations.

Specific growth rates and ethanol production from S. cerevisiae grown on YPAD medium containing 10 g/L of either
yeast extract (YE) or algae extract (AE). Each measurement represents the mean of triplicate
experiments standard deviations.

Concluding remarks :
Algae extract produced greater growth and metabolism saturation values as well
as prolonged growth and protein production in comparison to yeast extract
when used in complex media recipes for E. Coli cultures. When used as a
complex media supplement in S. Cerevisiae cultures, algae extract produced
superior growth and comparable ethanol production rates and saturation values.
The prolonged growth period afforded to yeast and bacteria cultures grown in
algae extract may be useful for some enzyme expression systems or secondary
metabolite production.

left to right: flasks containing the same concentration of Chlamydomonas

reinhardtii (i) wild strain CC 125 and mutants* (ii) tla1, (iii) 37RP1-tla1, (iv)
tlaX.