THC HANH NHP MN CNG NGH SINH HOC

ThS. Bui Thi Th

Nhom 4:
VN KIM MINH CHU THI HNG L NGUYN LAM QUYNH VN LUYN
QUANG TRUNG VN VN NH Y NGOC THNG THI TM

NHN GING CY LAN KIM IP

1: Lan KIM IP la loai lan rng Vi t Nam phn b chu yu vung ty nguyn.
ang ng trc nguy c tuy t chung ngoai thin nhin ( sach o Vi t Nam
phn II thc v t).
Nhn ging loai lan Kim ip ang co nguy c tuyt chung gop phn quan
trng bo tn ngun gene cua loai lan qu him nay; ng thi, tao ra ngun
nguyn liu quan trng ban u cho qu trnh sn xut hoa lan Kim ip gop phn
pht trin kinh t.
M cy chi bn mang inh sinh trng
2: V TRUNG MU
-dung xa phng ra di vi nc chy ( nc ct ) ( mu co nhiu bui t )
-dung cn 70 % s l mu (30 s) ri ra bng nc ct
-Dung dich kh trung : Dung HgCl2 1% di t khun mu ( cy lan co lp vo ngoai
bn (kitin) nn chi cn dung HgCl2 1% di t khun ) trong 10p ri dung nc ct
ra lai nhiu ln ( x l xong mu )
3: MI TRNG CY
MT nhn chi: MS + 30g/l sucrose + 8g agar + 2mg/l BAP + 1mg/l NAA +1g/l
than hoat tinh b sung 15% nc da ( b sung than hoat tinh di t khun )
MT tao r: MS + 20g/l sucrose + 8g agar + 1mg/l NAA