Anti-inflammatory effect of Glucocorticoid

[Purpose] Observe the anti-inflammatory effect of Glucocorticoid, grasp the method

to establish the inflammatory model of the ear of mice.
[Principle] The anti-inflammatory effect of Glucocorticoid is very strong, it is
effective on all kinds of inflammation and every stage. Dimethylbenzene and other
inflammatory substances was wiped on the ear of animals, it will damage part cells,
accelerate the release of histamine, bradykinin and other inflammatory substances,
lead to the acute edema in ear. The ear will become incrassation because of the
increasing permeability in blood capillary. We can judge the anti-inflammatory effect
of drugs.
[Materials]
1. Animals: Mouse, weight of 25-30 g, male.
2. Drugs: 0.5% hydrocortisone injection, dimethylbenzene, normal saline.
[Methods]
Two male mice every group, weight of 25-30 g, weigh and mark. Dimethylbenzene of
0.05-0.1 mL was wiped in the left ear (front and back) of the two mice. 30 mins later,
0.5% hydrocortisone injection was intraperitoneally injected into one mouse (0.1
mL/10 g), the same dose of normal saline was intraperitoneally injected into the other
mouse. The mice will be dislocated executed after administration for 1.5 hours. We
get the ear piece from right and left ears in the same place of every mouse using the
diameter of punch, then weigh them using the table balance. The ear piece weight
difference between left ear and right ear can evaluate the swollen degree. Summarize
the experimental data and record in the table.
[Results]
Anti-inflammatory effect of Glucocorticoid (ear piece of mice)
Group

Amount of
mice

hydrocortisone
Normal saline
[Notes]

Left ear

Average weight
Right ear
Swollen degree

The mice auricle inflammatory model could also be established using 2% croton oil
and 70% alcohol solution.
[Question]
Whats the anti-inflammatory mechanism of glucocorticoids.