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WHO AIDS Series 2 Guidelines on sterilization and disinfection methods effective against human immunodeficiency virus (HIV) Second edition @ WORLD HEALTH ORGANIZATION GENEVA 1989 Reprinted 1992 ‘WHO Library Cataloguing in Publication Data Guidelines on sterilization and disinfection methods effective against human immunodeti- ‘ency vis (HIV), (WHO AIDS series; 2) LHIV. 2.Acquired immunodeficiency syndrome - wansmission | 3,Equipment contami nation - prevention & control —4Sterlization - methods 5.Disinfection - methods Series ISBN 92.4 1212020 (NLM Classification: WD 308) ISSN 1011-573, (© World Health Organization 1989 Plains ofthe Weed Health Orzanization enjoy copyright protection n accordance with the provisions of Protocol 2 of the Universal Copyright Convention. All rights reserved. “The designations employed and the presentation of the material in this publication do not imply the expression of any opinion whatsoever on the part of the Secretariat ofthe World Health Organization conceraing the legal status of any Country, ternitory, city or area or of its authorities, or concerning the debimutation ofits frontiers or boundarss, ‘The mention of specific companies or of certain manufacturers’ produets does not imply that they are endorsed or recommended by the World Health Organization in preference to bthers of a similar nature that are not mentioned, Errors and omissions excepted, the znames of proprietary products are distinguished by initial capital letters. 52/92) Mebagaphin 2000 Contents Preface to the second edition Introduction HIV transmission Methods for sterilization and high-level disinfection of medical instruments Sterilization by steam Sterilization by dry heat High-level disinfection by boiling High-level disinfection by soaking in chemicals. Glutaral (glutaraldehyde) Hydrogen peroxide Field guide to sterilization and high-level disinfection of medical instruments Decontamination of environmental surtaces with chlorine-releasing compounds Sodium hypochlorite Calcium hypochlorite ‘Sodium dichloroisocyanurate Chioramine Disinfection of living tissues with antiseptics Ethanol and 2-propanol Polyvidone iodine Indicative prices of chemical agents Bibliography Preface to the second edition In the past year, considerable additional information has been developed ‘on sterilization and disinfection methods effective against the human im- munodeficiency virus (HIV), the causative agent of AIDS. This second edition of the guidelines takes this new information into count and clarifies several areas of possible confusion for users of the first edition. In this regard, the reader is invited to give special attention to the

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