D

M
t

2
1
0,5
0,3
1
1
1
1
30
30
30
30
Ln o
Ln o
1
Ln o 2 Ln o 3 Ln o 4 5
0,6045
0,6725
0,9055
0,9635
2,346

Mt quang

Ln o
1

Ln o
Ln o 2 Ln o 3 Ln o 4 5
0,08294 0,02651 0,01594
0,19206
9
4
3
0

Hot enzyme
(A)

f(x) =
R = 0

th chun nh lng protein

15
10
Abs

5
0
0

nng protein ( mg/ml)

10

12

 th biu th mt tng quan gia nng enzyme v hot ng enzyme

0.25
0.2
0.15
Hot enzyme (A)

f(x) = - 0.06x + 0.23

R = 0.88

0.1
0.05

0
0.5 1 1.5 2 2.5 3 3.5 4 4.5
CP1

CP2

CP3

CP4

f(x) =
Hm
R
=0

lng enzyme tch chit c so vi ng chun

12.000

EtOH

10.000

aceton

8.000
Abs

6.000
4.000
2.000
0.000
0

10

12