Kajian Kadar Total Lipid Dan Kepadatan Nitzschia Sp. Yang Dikultur Dengan

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}}}
}}\margt1700\margb200\margr800\margl1880
\plain\pard\sl-274 \li388 \ri386 \fi242 \expndtw-3 \b \f20 \fs24 KAJIAN KADAR TO
TAL LIPID DAN KEPADATAN\i NITZSCHIA SP\i0 . YANG
\plain\par
\pard\sl-276 \li1660 \ri1645 \fi187 \expndtw-4 \b \f20 \fs24 DIKULTUR DENGAN SAL
INITAS YANG BERBEDA
\plain\par
\sl-86\fs9\par\sl-240\fs24\par\pard\pard\sl-271 \li517 \ri1312 \fi1142 \expndtw3 \b \f20 \fs24 Widianingsih; Retno Hartati; Hadi Endrawati dan Hilal M.
\plain\par
\pard\sl-271 \li388 \ri286 \fi129 \expndtw-2 \f20 \fs24 Jurusan Ilmu Kelautan, F
akultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro
\plain\par
\pard\sl-276 \li388 \ri889 \fi799 \expndtw-2 \f20 \fs24 Kampus UNDIP Tembalang S
emarang E-mail:{\field{\*\fldinst {\b HYPERLINK}{\b "mailto:Widia2506@Yahoo.com"
}}
{\fldrslt{\cf6 Widia2506@Yahoo.com
}}}
\plain\par
\sl-315\fs31\par\pard\pard\sl-220 \li388 \ri3714 \fi3938 \expndtw-10 \b \f20 \fs
22 Abstrak
\plain\par
\pard\sl-223 \li388 \ri167 \expndtw0 \i \f20 \fs19 Penelitian ini bertujuan untu
k mempelajari kadar total lipid dan kepadatan pada mikroalga Nitzschia sp.
\plain\par
\pard\sl-230 \li388 \ri167 \expndtw0 \i \f20 \fs19 Penelitian ini menggunakan ra
ncangan acak lengkap dengan perlakuan salinitas 15, 20, 25, 30, dan 35
\plain\expndtw0 \i \f20 \fs19 ppt (3 kali ulangan). . Nitzschia sp. dikultur pad
a skala laboratorium dengan pupuk F/2 dan dilakukan
\par
\plain\pard\sl-354 \li388 \ri165 \expndtw0 \i \f20 \fs19 pemanenan setelah menca
pai fase stasioner untuk kemudian diukur kadar total lipid. Perlakuan\fs12 6\fs
19 salinitas
\plain\par
\pard\sl-106 \li388 \ri168 \expndtw0 \i \f20 \fs19 menunjukkan bahwa rata-rata k
epadatan Nitzschia sp. tertinggi pada salinitas 20 ppt (37,32 x 10 sel/mL)
\plain\par
\pard\sl-227 \li387 \ri167 \expndtw2 \i \f20 \fs19 dan rata-rata kepadatan teren
dah pada salinitas 30 ppt 1(8,62 x10\up5 \fs12 6\up0 \fs19 sel/mL). Berdasarkan
hasil
\plain\expndtw0 \i \f20 \fs19 pengamatan diperoleh nilai rata-rata persentase ka
dar total lipid tertinggi terdapat pada salinitas 35 ppt
\plain\expndtw0 \i \f20 \fs19 (71,51 5,35 %-dw) dan persentase rata-rata kadar t
otal lipid terendah terdapat pada salinitas 15 ppt
\plain\expndtw0 \i \f20 \fs19 (13,26 0.47 %-dw). Dari hasil penelitian dapat dis
impulkan bahwa Nitzschia sp. dapat tumbuh pada
\plain\expndtw-1 \i \f20 \fs19 salinitas 15 - 35 ppt dengan kepadatan tertinggi
pada salinitas 20 ppt dan rata- rata persentase kadar total
\plain\expndtw-2 \i \f20 \fs19 lipid tertinggi pada salinitas 30 ppt.
\plain\par
\sl-259\fs26\par\pard\pard\sl-199 \li388 \ri3047 \expndtw-1 \b\i \f20 \fs19 Kata
-kata kunci\b0 : Nitzschia sp., densitas, salinitas, kadar total lipid.
\plain\par
\sl-111\fs11\par\sl-240\fs24\par\par\pard\pard\sl-240 \li388 \ri3688 \fi3912 \ex
pndtw-9 \b\i \f20 \fs24 Abstract
\plain\par
\pard\sl-226 \li388 \ri168 \expndtw-1 \i \f20 \fs19 This research aimed to study
the growth and total lipid content in microalgae Nitzschia sp. This study used
\plain\par
\pard\sl-230 \li387 \ri166 \expndtw4 \i \f20 \fs19 a Complete Randomized Design
with treatments of salinity (15, 20, 25, 30, and 35 ppt (3
\plain\expndtw0 \i \f20 \fs19 replications). Nitzschia sp. were cultured on labo
ratory scale and harvested after reaching stationary
\plain\expndtw0 \i \f20 \fs19 phase and then analyzed for total lipid content. T
he result showed that the average density of Nitzschia sp.
\plain\expndtw0 \i \f20 \fs19 was highest at 20 ppt salinity (37.32 x 10\up5 \fs
12 6\up0 \fs19 cells/mL) and an average density of the lowest at 30 ppt salinit
y
\plain\expndtw0 \i \f20 \fs19 was 18.62 x10\up5 \fs12 6\up0 \fs19 cells/mL. Bas
ed on observations obtained an average percentage of total lipid levels was
\plain\expndtw-1 \i \f20 \fs19 highest in the salinity of 35 ppt (71.51 5.35% dw
) and the average percentage of the lowest levels of total
\plain\expndtw0 \i \f20 \fs19 lipids contained in the salinity of 15 ppt (13.26
00:47 %-dw). From the results of this study concluded
\plain\expndtw0 \i \f20 \fs19 that Nitzschia sp. can growth in salinity 15 - 35
ppt with optimal level of density in 20 ppt and the average
\plain\expndtw-1 \i \f20 \fs19 percentage of optimal levels of total lipid prese
nt in 30 ppt salinity.
\plain\par
\sl-261\fs26\par\pard\pard\sl-199 \li388 \ri4149 \expndtw-2 \b\i \f20 \fs19 Keyw
ords\b0 : Nitzschia sp., density, salinity, total lipid.
\par
\sect\sectd\sbknone\cols2\colno1\colw4245\colsr714\colno2\colw4266
\plain\sl-110\fs11\par\sl-240\fs24\par\par\pard\pard\sl-220 \li11 \ri2522 \expnd
tw-8 \b \f20 \fs22 PENDAHULUAN
\plain\par
\sl-249\fs25\par\pard\pard\sl-251 \li11 \ri2 \fi719 \expndtw2 \i \f20 \fs22 Nitz
schia\i0 sp. merupakan mikroalga
\plain\par
\pard\sl-251 \li11 \ri0 \expndtw0 \f20 \fs22 yang termasuk dalam kelas{\field{\*
\fldinst {\b HYPERLINK}{\b "http://en.wikipedia.org/wiki/Diatom"}}
{\fldrslt{ Bacillariophyceae
}}}
\plain\expndtw-2 \f20 \fs22 (Tomas, 1997).\i Nitzschia\i0 sp. mempunyai peran
\plain\expndtw-1 \f20 \fs22 yang penting dalam ekosistem perairan sebagai
\plain\expndtw4 \f20 \fs22 produsen primer. Mikroalga ini banyak
\plain\expndtw2 \f20 \fs22 digunakan sebagai pakan alami bagi larva
\plain\expndtw0 \f20 \fs22 organisme laut seperti krustacea, bivalvia, dan
\plain\expndtw-3 \f20 \fs22 ikan (Isnansetyo dan Kurniastuty, 1995).
\plain\par
\pard\sl-251 \li11 \ri0 \fi720 \expndtw4 \f20 \fs22 Selain sebagai pakan alami b
agi
\plain\par
\pard\sl-251 \li11 \ri1 \expndtw-1 \f20 \fs22 organisme laut, mikroalga Nitzschi
a juga salah
\plain\expndtw2 \f20 \fs22 satu jenis organisme penghasil lipid yang
\plain\expndtw0 \f20 \fs22 potensial untuk dikembangkan sebagai bahan
\plain\expndtw1 \f20 \fs22 dasar pembuatan biodiesel (Campbell\i et al\i0 .,
\plain\expndtw3 \f20 \fs22 2008). Pengembangan mikroalga sebagai
\par
\column
\plain\sl-313\fs31\par\sl-240\fs24\par\pard\pard\sl-251 \li0 \ri31 \expndtw4 \f2
0 \fs22 sumber biodiesel mempunyai beberapa
\plain\expndtw-3 \f20 \fs22 keunggulan, yaitu kecepatan pertumbuhan yang
\plain\expndtw5 \f20 \fs22 tinggi sehingga masa panennya cepat
\plain\expndtw4 \f20 \fs22 (Andersen, 2005) dibandingkan dengan
\plain\expndtw0 \f20 \fs22 tanaman perkebunan lainnya seperti jarak dan
\plain\expndtw0 \f20 \fs22 sawit, mikroalga mempunyai kandungan lipid
\plain\expndtw2 \f20 \fs22 yang tinggi (Christi, 2007), bersifat ramah
\plain\expndtw0 \f20 \fs22 lingkungan, nilai emisinya rendah, dan dapat
\plain\expndtw-6 \f20 \fs22 diperbarui.
\plain\par
\pard\sl-254 \li0 \ri31 \fi708 \expndtw4 \f20 \fs22 Salah satu jenis mikroalga y
ang
\plain\par
\pard\sl-251 \li0 \ri29 \expndtw0 \f20 \fs22 potensial sebagai bahan dasar biodi
esel adalah
\plain\expndtw4 \i \f20 \fs22 Nitzschia\i0 sp. Mikroalga ini mempunyai
\plain\expndtw1 \f20 \fs22 kecepatan pertumbuhan yang tinggi, mudah
\plain\expndtw-1 \f20 \fs22 dilakukan pembudidayaan, dan memiliki kadar
\plain\expndtw0 \f20 \fs22 lipid yang cukup tinggi (Chisti, 2007). Hal ini
\plain\expndtw5 \f20 \fs22 terkait dengan senyawa karbon yang
\plain\par
\sl-255\fs26\par\sl-240\fs24\par\pard\pard\sl-240 \li0 \ri167 \fi3840 \expndtw-2
7 \f20 \fs24 29
\par
\sect\sectd\sbknone\sect\sectd\sbkpage\margtsxn1620\pard
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}}}
\cols2\colno1\colw4246\colsr713\colno2\colw4266
\plain\pard\sl-268 \li11 \ri2 \expndtw1 \f20 \fs22 terkandung dalam ekstrak lipi
dnya sebagian
\plain\par
\pard\sl-251 \li11 \ri2 \expndtw5 \f20 \fs22 besar disimpan dalam bentuk minyak
\plain\expndtw-3 \f20 \fs22 (trigliserida) maupun asam lemak jenuh (Thorn,
\plain\expndtw-11 \f20 \fs22 2007).
\plain\par
\pard\sl-251 \li10 \ri2 \fi708 \expndtw1 \f20 \fs22 Penelitian mengenai mikroalg
a laut
\plain\par
\pard\sl-254 \li10 \ri3 \expndtw0 \f20 \fs22 sebagai penghasil lipid perlu dilak
ukan untuk
\plain\expndtw-2 \f20 \fs22 mencari jenis mikroalga laut yang potensial dan
\plain\expndtw1 \f20 \fs22 memiliki kandungan lipid yang besar untuk

\plain\expndtw1 \f20 \fs22 pengembangan biodiesel. Namun demikian
\plain\expndtw0 \f20 \fs22 terdapat beberapa kesulitan untuk menemukan
\plain\expndtw-1 \f20 \fs22 strain mikroalga yang mengandung lipid tinggi
\plain\expndtw2 \f20 \fs22 dengan pertumbuhan cepat sehingga biaya
\plain\expndtw0 \f20 \fs22 operasional pemanenan rendah dan diperoleh
\plain\expndtw-4 \f20 \fs22 sistem kultur yang efektif.
\plain\par
\pard\sl-251 \li10 \ri1 \fi708 \expndtw-2 \f20 \fs22 Beberapa mikroalga dapat hi
dup dalam
\plain\par
\pard\sl-254 \li10 \ri5 \expndtw4 \f20 \fs22 kondisi lingkungan yang penuh denga
n
\plain\expndtw3 \f20 \fs22 tekanan. Hal ini berarti mikroalga dapat
\plain\expndtw3 \f20 \fs22 dieksploitasi secara bioteknologi. Faktor
\par
\column
\plain\pard\sl-266 \li0 \ri32 \expndtw1 \f20 \fs22 bahwa salinitas mempunyai pen
garuh besar
\plain\par
\pard\sl-251 \li0 \ri31 \expndtw2 \f20 \fs22 terhadap kandungan lipid yang dihas
ilkan
\plain\expndtw1 \f20 \fs22 mikroalga. Maka perlu dilakukan penelitian
\plain\expndtw2 \f20 \fs22 mengenai pengaruh salinitas media kultur
\plain\expndtw1 \f20 \fs22 terhadap pertumbuhan dan kadar total lipid.
\plain\expndtw-1 \f20 \fs22 Penelitian ini bertujuan untuk mempelajari dan
\plain\expndtw1 \f20 \fs22 mengetahui pengaruh salinitas yang berbeda
\plain\expndtw1 \f20 \fs22 terhadap kepadatan sel dan kadar total lipid
\plain\expndtw-4 \f20 \fs22 pada mikroalga\i Nitzschia\i0 sp.
\plain\par
\sl-263\fs26\par\pard\pard\sl-247 \li0 \ri2220 \expndtw-5 \b \f20 \fs22 Bahan da
n Metoda
\plain\par
\pard\sl-247 \li0 \ri31 \fi708 \expndtw0 \f20 \fs22 Penelitian ini dilaksanakan
pada bulan
\plain\par
\pard\sl-254 \li0 \ri29 \expndtw1 \f20 \fs22 Juli - Agustus 2011. Pelaksanaan pe
nelitian
\plain\expndtw3 \f20 \fs22 dilakukan di Laboratorium Biologi laut,
\plain\expndtw-1 \f20 \fs22 Jurusan Ilmu Kelautan, Fakultas Perikanan dan
\plain\expndtw4 \f20 \fs22 Ilmu Kelautan Universitas Diponegoro.
\plain\expndtw-1 \i \f20 \fs22 Nitzschia\i0 sp. Yang digunakan dalam penelitian
\plain\expndtw0 \f20 \fs22 ini merupakan stok murni Laboratorium Balai
\par
\plain\pard\sl-251 \li11 \ri0 \expndtw0 \f20 \fs22 lingkungan yang sangat berpen
garuh terhadap
\plain\expndtw0 \f20 \fs22 produksi lipid adalah salinitas (Derenne\i et al.,
\plain\expndtw3 \f20 \fs22 1992), fotoperiod, dan intensitas cahaya
\plain\expndtw0 \f20 \fs22 (Brenckmann\i et al.,\i0 1985), nitrogen, dan suhu
\plain\expndtw3 \f20 \fs22 (Lupi{\field{\*\fldinst {\b HYPERLINK}{\b "https://ww
w.researchgate.net/publication/227226572_Temperature_profiles_of_cellular_growth
_and_exopolysaccharide_synthesis_by_Botryococus_braunii_Kutz_UC_58?el=1_x_8&enri
chId=rgreq-108e4bcb-c682-4c70-8c49-884ede4642e3&enrichSource=Y292ZXJQYWdlOzI3Nzg
3MDEwODtBUzoyNjU4OTc2MDQ1NDY1NjFAMTQ0MDQwNjMzNzU0Ng=="}}
{\fldrslt{\i et al.,\i0 1991).
}}} Oleh karena itu perlu
\plain\expndtw0 \f20 \fs22 ditetapkan kondisi lingkungan yang memadai

\plain\expndtw0 \f20 \fs22 agar didapatkan pertumbuhan mikroalga dan
\plain\expndtw-4 \f20 \fs22 kadar lipid yang optimum.
\par
\column
\plain\pard\sl-386 \li0 \ri31 \expndtw-1 \f20 \fs22 Besar Pengembangan Budidaya
Laut Lampung\fs13 6
\plain\par
\pard\sl-115 \li0 \ri92 \expndtw2 \f20 \fs22 (BBPBLL), dengan kepadatan 13,50 x
10
\plain\par
\pard\sl-257 \li0 \ri3241 \expndtw-10 \f20 \fs22 sel/mL.
\plain\par
\pard\sl-251 \li0 \ri30 \fi708 \expndtw0 \f20 \fs22 Sebelum dilakukan penelitian
terlebih
\plain\par
\pard\sl-254 \li0 \ri28 \expndtw1 \f20 \fs22 dahulu dilakukan sterilisasi ruang,
peralatan
\plain\expndtw-2 \f20 \fs22 dan media kultur. Air laut yang akan digunakan
\plain\expndtw0 \f20 \fs22 terlebih dahulu disterilisasi dengan klorin 60
\plain\expndtw3 \f20 \fs22 ppm (mg/L) dan diaerasi selama 24 jam,
\par
\sect\sectd\sbknone\plain\pard\sl-251 \li719 \ri29 \expndtw0 \i \f20 \fs22 Nitzs
chia\i0 sp. mengandung protein 33
\plain\tx4960 \tab \expndtw-9 \f20 \fs22 kemudian
\plain\tx6208 \tab \expndtw0 \f20 \fs22 untuk menetralisir kadar klorin
\par
\plain\pard\sl-253 \li10 \ri0 \expndtw0 \f20 \fs22 %; lemak 21 %; dan karbohidra
t 28 % (Ben\plain\expndtw2 \f20 \fs22 Amotz\i and\i0 Fishler, 1990). Lipid berfungsi
\plain\expndtw1 \f20 \fs22 sebagai sumber energi cadangan apabila sel
\plain\expndtw0 \f20 \fs22 kekurangan karbohidrat sebagai sumber energi
\plain\expndtw0 \f20 \fs22 utama. Walaupun sedikit kandungan dari total
\plain\expndtw0 \f20 \fs22 lipid, namun salah satu penelitian Salah satu
\par
\column
\plain\pard\sl-268 \li0 \ri27 \expndtw1 \f20 \fs22 pada air laut digunakan Na\dn
4 \fs13 2\dn0 \fs22 S\dn4 \fs13 2\dn0 \fs22 O\dn4 \fs13 3\dn0 \fs22 (Natrium
\plain\par
\pard\sl-238 \li0 \ri31 \expndtw1 \f20 \fs22 Thiosulfat) 30 mg/L dan diaerasi se
lama 24
\plain\par
\pard\sl-254 \li0 \ri34 \expndtw5 \f20 \fs22 jam ppm, kemudian air laut tersebut
\plain\expndtw0 \f20 \fs22 disterilisasi dengan dipaparkan UV selama 2-3
\plain\expndtw-16 \f20 \fs22 jam.
\plain\par
\pard\sl-255 \li0 \ri33 \fi763 \expndtw-1 \f20 \fs22 Rancangan percobaan yang di
gunakan
\par
\sect\sectd\sbknone\plain\pard\sl-251 \li10 \ri28 \expndtw-2 \f20 \fs22 cara unt
uk meningkatkan kandungan lipid pada
\plain\tx4959 \tab \expndtw1 \f20 \fs22 dalam penelitian adalah
\plain\tx7677 \tab \expndtw0 \f20 \fs22 rancangan acak
\par
\plain\pard\sl-254 \li10 \ri31 \expndtw2 \i \f20 \fs22 Nitzschia\i0 sp. adalah
dengan memanipulasi
\plain\tx4946 \tab \expndtw0 \f20 \fs22 lengkap dengan perlakuan salinitas 15, 2
0, 25,
\plain\expndtw-2 \f20 \fs22 faktor lingkungan, salah satunya adalah dengan
\plain\tx4946 \tab \expndtw1 \f20 \fs22 30, 35 ppt dengan 3 kali pengulangan. Pa
da
\plain\expndtw1 \f20 \fs22 perlakuan salinitas dalam kultur mikroalga.
\plain\tx4946 \tab \expndtw1 \f20 \fs22 penelitian ini media kultur dipupuk deng
an
\plain\expndtw1 \f20 \fs22 Media kultur yang didukung dengan media,
\plain\tx4946 \tab \expndtw3 \f20 \fs22 pupuk F/2 dengan komposisi (Tabel 1.);
\par
\plain\pard\sl-251
ng baik diharapkan
\plain\tx4959 \tab
\plain\tx5989 \tab
\plain\tx6697 \tab
\plain\tx7662 \tab
\plain\tx8536 \tab
\li10 \ri31 \expndtw-1 \f20 \fs22 suhu, nutrien dan cahaya ya

\expndtw-9 \f20 \fs22 Larutan
\expndtw-16 \f20 \fs22 stok
\expndtw-9 \f20 \fs22 nutrien
\expndtw-11 \f20 \fs22 dibuat
\expndtw-12 \f20 \fs22 dengan
\par
\plain\pard\sl-254 \li10 \ri31 \expndtw-2 \f20 \fs22 dapat menghasilkan pertumbu
han yang optimal
\plain\tx4946 \tab \expndtw3 \f20 \fs22 menambahkan 1 liter air laut yang telah
\plain\par
\pard\sl-251 \li10 \ri2885 \expndtw0 \f20 \fs22 dan kadar lipid tinggi. Al-Hasan
\i et al\i0 ., (1990)
\plain\tx4946 \tab \expndtw-5 \f20 \fs22 disterilisasi.
\plain\par
\pard\sl-250 \li10 \ri4485 \expndtw3 \f20 \fs22 dan Renaud & Perry, (1994) melap
orkan
\par
\plain\sl-287\fs29\par\pard\pard\sl-220 \li1107 \ri2112 \expndtw-2 \b \f20 \fs22
T\b0 abel\b 1\b0 . Komposisi Nutrien F/2 pada Media Kultur\i Nitzschia\i0 sp
.
\par
\plain\pard\sl-286 \li1828 \ri3491 \expndtw-9 \f20 \fs22 Komponen
\plain\tx4694 \tab \expndtw-11 \f20 \fs22 Jumlah
\par
\plain\pard\sl-294 \li1240 \ri3745 \expndtw-10 \f20 \fs22 NaNO\dn4 \fs13 3
\plain\tx4694 \tab \expndtw-8 \f20 \fs22 84,148 g
\par
\sect\sectd\sbknone\cols3\colno1\colw2953\colsr1754\colno2\colw276\colsr443\coln
o3\colw3798
\plain\pard\sl-254 \li1240 \ri253 \expndtw-7 \f20 \fs22 NaH\dn4 \fs13 2\dn0 \fs2
2 PO\dn4 \fs13 4\dn0 \fs22 .2H\dn4 \fs13 2\dn0 \fs22 O
\plain\par
\pard\sl-251 \li1240 \ri844 \expndtw-7 \f20 \fs22 Na\dn4 \fs13 2\dn0 \fs22 SiO\d
n4 \fs13 3
\plain\par
\pard\sl-238 \li1240 \ri471 \expndtw-7 \f20 \fs22 FeCl3.6 H2O
\plain\par
\pard\sl-254 \li1240 \ri756 \expndtw-10 \f20 \fs22 Na2EDTA

\plain\par
\pard\sl-252 \li1240 \ri0 \expndtw-4 \f20 \fs22 Larutan trace metal
\par
\column
\plain\pard\sl-240 \li0 \ri49 \expndtw-25 \f20 \fs22 10
\plain\par
\plain\par
\pard\sl-251 \li0 \ri0 \expndtw-20 \f20 \fs22 2,9
\plain\expndtw-25 \f20 \fs22 10
\plain\par
\par
\column
\plain\pard\sl-240 \li0 \ri3319 \expndtw-2 \f20 \fs22 g
\plain\par
\pard\sl-251 \li0 \ri3319 \expndtw-2 \f20 \fs22 g
\plain\expndtw-2 \f20 \fs22 g
\plain\expndtw-2 \f20 \fs22 g
\plain\par
\pard\sl-252 \li0 \ri3495 \expndtw-26 \f20 \fs22 mL
\par
\sect\sectd\sbknone\plain\sl-220\fs22\par\sl-240\fs24\par\par\par\pard\pard\sl-2
40 \li8951 \ri31 \expndtw-27 \f20 \fs24 30
\par
{\shp
ffd8ffe000104a46494600010100000100010000ffdb004300010101010101010101010101010101
01010101010101010101010101010101010101010101010101010101010101010101010101010101
010101010101010101ffdb0043010101010101010101010101010101010101010101010101010101
0101010101010101010101010101010101010101010101010101010101010101010101010101ffc0
00110806da04d803012200021101031101ffc4001f00000105010101010101000000000000000001
02030405060708090a0bffc400b5100002010303020403050504040000017d010203000411051221
31410613516107227114328191a1082342b1c11552d1f02433627282090a161718191a2526272829
2a3435363738393a434445464748494a535455565758595a636465666768696a737475767778797a
0101010101010101010000000000000102030405060708090a0bffc400b511000201020404030407
05040400010277000102031104052131061241510761711322328108144291a1b1c109233352f015
6272d10a162434e125f11718191a262728292a35363738393a434445464748494a53545556575859
5a636465666768696a737475767778797a82838485868788898a92939495969798999aa2a3a4a5a6
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a0028afe707f6f5ff00835fbf605ff828
97ed63f15bf6c5f8d7f177f6bff0bfc4df8c1ff082ff00c24da17c2df1f7c17d13c0963ff0affe1b
783be16e8dfd85a678b3e0078dbc416df69f0ff8274abcd4ff00b43c4fa9f9dac5c6a1716bf62b29
6db4fb4f903fe20a9ff82597fd17cfdbff00ff000e9fecebff00d0ab401fd7ed15fc817fc4153ff0
4b2ffa2f9fb7ff00fe1d3fd9d7ff00a1568ff882a7fe0965ff0045f3f6ff00ff00c3a7fb3aff00f4
2ad007f5fb457f205ff1054ffc12cbfe8be7edff00ff00874ff675ff00e855a3fe20a9ff0082597f
d17cfdbfff00f0e9fecebffd0ab401fd7ed15fc817fc4153ff0004b2ff00a2f9fb7fff00e1d3fd9d
7ffa1568ff00882a7fe0965ff45f3f6fff00fc3a7fb3afff0042ad007f5fb457f205ff001054ff00
c12cbfe8be7edfff00f874ff00675ffe855a3fe20a9ff82597fd17cfdbff00ff000e9fecebff00d0
ab401fd7ed15fc817fc4153ff04b2ffa2f9fb7ff00fe1d3fd9d7ff00a1568ff882a7fe0965ff0045
f3f6ff00ff00c3a7fb3aff00f42ad007f5fb457f205ff1054ffc12cbfe8be7edff00ff00874ff675
ff00e855a3fe20a9ff0082597fd17cfdbfff00f0e9fecebffd0ab401fd7ed15fc817fc4153ff0004
b2ff00a2f9fb7fff00e1d3fd9d7ffa1568ff00882a7fe0965ff45f3f6fff00fc3a7fb3afff0042ad
007f5fb457f205ff001054ff00c12cbfe8be7edfff00f874ff00675ffe855a3fe20a9ff82597fd17
cfdbff00ff000e9fecebff00d0ab401fd7ed15fc817fc4153ff04b2ffa2f9fb7ff00fe1d3fd9d7ff
00a1568ff882a7fe0965ff0045f3f6ff00ff00c3a7fb3aff00f42ad007f5fb457f205ff1054ffc12
cbfe8be7edff00ff00874ff675ff00e855a3fe20a9ff0082597fd17cfdbfff00f0e9fecebffd0ab4
01fd7ed15fc817fc4153ff0004b2ff00a2f9fb7fff00e1d3fd9d7ffa1568ff00882a7fe0965ff45f
3f6fff00fc3a7fb3afff0042ad007f5fb457f205ff001054ff00c12cbfe8be7edfff00f874ff0067
5ffe855a3fe20a9ff82597fd17cfdbff00ff000e9fecebff00d0ab401fd7ed15fc817fc4153ff04b
2ffa2f9fb7ff00fe1d3fd9d7ff00a156bebffd82bfe0d7efd817fe09dbfb58fc29fdb17e0a7c5dfd
affc51f137e0ff00fc275ff08ce85f14bc7df05f5bf025f7fc2c0f86de31f85bacff006ee99e13f8
01e09f105cfd9bc3fe36d56f34cfecff0013e99e4eb16fa7dc5d7db6ca2b9d3eec03fa3ea28a2800
a28a2800a28a2800a28a2800a28a2800a28a2803ffd9
}}}
\plain\pard\sl-268 \li11 \ri21 \expndtw-9 \f20 \fs22 Larutan
\plain\tx1069 \tab \expndtw-13 \f20 \fs22 trace
\plain\tx1869 \tab \expndtw-13 \f20 \fs22 metal
\plain\tx2729 \tab \expndtw-11 \f20 \fs22 dibuat
\plain\tx3653 \tab \expndtw-12 \f20 \fs22 dengan
\plain\tx4991 \tab \expndtw0 \f20 \fs22 dengan perlakuan salinitas 15, 20, 25, 3
0 dan
\par
\plain\pard\sl-251 \li11 \ri21 \expndtw1 \f20 \fs22 menambahkan komponen Trace M
etal yang
\plain\tx4991 \tab \expndtw1 \f20 \fs22 35 ppt (3 x ulangan) yang sebelumnya tel
ah
\plain\expndtw0 \f20 \fs22 meliputi ZnSO4.7 H2O (2 g) ; CuSO4.5 H2O
\plain\tx4991 \tab \expndtw3 \f20 \fs22 diberi pupuk dengan konsentrasi 1 ml/L.
\par
\plain\pard\sl-265 \li11 \ri21 \expndtw-4 \f20 \fs22 (1,96 g) ; CoCl2.6 H2O (2
\plain\tx2891 \tab \expndtw6 \f20 \fs22 g) (NH4) 6
\plain\tx4991 \tab \expndtw0 \f20 \fs22 Kepadatan awal (t\dn4 \fs13 0\dn0 \fs22
) Nitzschia sebesar 4,50 x
\par
\plain\pard\sl-238 \li11 \ri21 \expndtw-1 \f20 \fs22 Mo7O24..4 H2O (1,26 g). Lar
utan stok vitamin
\plain\tx4991 \tab \expndtw3 \f20 \fs22 10\up6 \fs13 6\up0 \fs22 sel/mL. Pencah
ayaan secara kontinyu
\plain\par
\pard\sl-254 \li11 \ri21 \expndtw0 \f20 \fs22 dibuat dengan menambahkan vitamin
pada 100
\plain\tx4991 \tab \expndtw5 \f20 \fs22 dengan lampu TL 40 watt/m2 dengan
\plain\expndtw-1 \f20 \fs22 mL air destilasi dengan komposisi; Vitamin B1
\plain\tx4991 \tab \expndtw3 \f20 \fs22 fotoperiod 24 jam. Aerasi juga dilakukan
\par
\plain\pard\sl-254
\plain\tx2929 \tab
\plain\tx5005 \tab
\plain\tx8194 \tab
\li11 \ri20 \expndtw2 \f20 \fs22 (Thiamin HCl) 0,2 mg;

\expndtw3 \f20 \fs22 Vitamin B12
\expndtw4 \f20 \fs22 kontinyu selama 24 jam.
\expndtw-6 \f20 \fs22 Perhitungan
\par
\plain\pard\sl-251 \li11 \ri21 \expndtw1 \f20 \fs22 (Cobalamin) 10 mg dan Vitami
n H (Biotin)
\plain\tx5005 \tab \expndtw-8 \f20 \fs22 kepadatan
\plain\tx6474 \tab \expndtw3 \f20 \fs22 dilakukan dengan bantuan
\par
\plain\pard\sl-251
\plain\tx4991 \tab
ter
\plain\par
\pard\sl-254 \li11
a erlenmeyer
\plain\tx4991 \tab
\li11 \ri21 \expndtw-2 \f20 \fs22 10 mg

\expndtw0 \f20 \fs22 mikroskop binokuler dan alat Haemocytome
\ri20 \fi708 \expndtw-3 \f20 \fs22 Media kultur disiapkan pad
\expndtw2 \f20 \fs22 dengan perbesaran 400 x dengan rumusan
\par
\plain\pard\sl-251 \li11 \ri642 \expndtw2 \f20 \fs22 yang bervolume 500 mL seban
yak 200 mL
\plain\tx4991 \tab \expndtw-3 \f20 \fs22 Seafdec (1985)\i dalam\i0 Taw (1990)
yaitu:
\plain\par
\sl-249\fs25\par\pard\pard\sl-240 \li11 \ri5186 \fi1440 \expndtw-4 \f20 \fs24 Jm
l sel dalam 4 kotak
\par
\sect\sectd\sbknone\cols4\colno1\colw1279\colsr171\colno2\colw1279\colsr1060\col
no3\colw500\colsr713\colno4\colw4301
\plain\pard\sl-273 \li11 \ri0 \expndtw-7 \f20 \fs24 Jml sel/mL =
\par
\column
\plain\sl-112\fs11\par\sl-240\fs24\par\par\pard\pard\sl-240 \li0 \ri0 \expndtw-6
\f20 \fs24 Jml kotak (4)
\par
\column
\plain\pard\sl-273 \li0 \ri0 \expndtw-9 \f20 \fs24 x 10\up6 \fs16 4
\par
\column
\plain\sl-271\fs27\par\sl-240\fs24\par\pard\pard\sl-251 \li0 \ri23 \expndtw0 \f2
0 \fs22 perlakuan analisis kadar total lipid, sedangkan
\plain\expndtw-1 \f20 \fs22 sisa 100 mL masih tetap dilakukan kultur untuk
\plain\expndtw-5 \f20 \fs22 hari selanjutnya.
\plain\par
\pard\sl-251 \li0 \ri20 \fi720 \expndtw2 \f20 \fs22 Analisa kadar total lipid di
lakukan
\par
\plain\pard\sl-252 \li11 \ri0 \expndtw1 \f20 \fs22 Pengambilan sampel untuk kada
r total lipid
\plain\expndtw2 \f20 \fs22 dilakukan setelah mikroalga\i Nitzschia\i0 sp.
\plain\expndtw0 \f20 \fs22 mencapai fase stasioner atau kepadatan puncak
\plain\expndtw-1 \f20 \fs22 yang jatuh pada hari kedelapan. Dimana setelah
\plain\expndtw3 \f20 \fs22 penghitungan kepadatan terakhir, sampel
\plain\expndtw0 \i \f20 \fs22 Nitzschia\i0 sp. diambil 100 mL untuk dilakukan
\plain\par
\sl-239\fs24\par\pard\pard\sl-251 \li802 \ri1293 \fi1375 \expndtw-10 \f20 \fs22
(A - B)
\plain\par
\pard\sl-251 \li11 \ri366 \fi791 \expndtw0 \f20 \fs22 % Total lipid = ------------- x 100
\plain\par
\pard\sl-254 \li387 \ri1593 \fi2160 \expndtw-3 \f20 \fs22 C
\plain\par
\sl-285\fs29\par\sl-240\fs24\par\pard\pard\sl-220 \li11 \ri2541 \fi376 \expndtw7 \f20 \fs22 Keterangan;
\par
\column
\plain\pard\sl-251 \li0 \ri22 \expndtw-1 \f20 \fs22 sesuai dengan metode Bethien
- Diemar (1963)
\plain\expndtw0 \i \f20 \fs22 dalam\i0 Hermawan 2004. dengan menggunakan
\plain\expndtw0 \f20 \fs22 pelarut non-polar petroleum ether. Perhitungan
\plain\expndtw2 \f20 \fs22 prosentase berat kering total lipid sebagai
\plain\par
\pard\sl-251 \li0 \ri3253 \expndtw-8 \f20 \fs22 berikut:
\par
\sect\sectd\sbknone\plain\pard\sl-372 \li387 \ri3317 \expndtw-3 \f20 \fs22 A = B
erat labu + berat lipid setelah dilakukan ekstrasi (mg)
\plain\par
\pard\sl-372 \li387 \ri4460 \expndtw-8 \f20 \fs22 B = Berat labu sebelum dilakuk
an ekstrasi (mg)
\plain\expndtw-4 \f20 \fs22 C = Berat kering sampel.
\par
\plain\sl-134\fs13\par\sl-240\fs24\par\pard\pard\sl-251 \li10 \ri0 \fi595 \expnd
tw3 \f20 \fs22 Kadar total lipid dinyatakan dalam
\plain\par
\pard\sl-251 \li10 \ri1 \expndtw0 \f20 \fs22 persentase kadar total lipid. Selan
jutnya hasil
\plain\expndtw0 \f20 \fs22 analisis dibandingkan antar perlakuan dengan
\plain\expndtw5 \f20 \fs22 Anova satu arah SPSS versi 16,0 yang
\plain\expndtw2 \f20 \fs22 sebelumnya dilakukan uji normalitas untuk
\par
\column
\plain\sl-138\fs14\par\sl-240\fs24\par\pard\pard\sl-251 \li0 \ri1931 \expndtw-5
\b \f20 \fs22 Hasil dan Pembahasan
\plain\par
\pard\sl-251 \li0 \ri22 \fi330 \expndtw-1 \b \f20 \fs22 Pengaruh Salinitas Terha
dap Kepadatan
\plain\expndtw-6 \b\i \f20 \fs22 Nitzschia\i0 sp.
\plain\par
\pard\sl-249 \li0 \ri23 \fi708 \expndtw1 \f20 \fs22 Hasil penelitian menunjukkan
bahwa
\plain\par
\pard\sl-251 \li0 \ri22 \expndtw0 \f20 \fs22 rata-rata kepadatan\i Nitzschia\i0
sp. tertinggi pada
\par
\plain\pard\sl-254 \li10 \ri0 \expndtw0 \f20 \fs22 mengetahui distribusi data be
rsifat normal. Hal
\plain\expndtw2 \f20 \fs22 ini dilakukan agar tepat dalam menentukan
\plain\expndtw2 \f20 \fs22 pemilihan uji statistik yang digunakan. Uji
\plain\expndtw-3 \f20 \fs22 homogenitas adalah uji mengenai sama tidaknya
\plain\expndtw0 \f20 \fs22 variansi dua buah distribusi atau lebih (Fowler
\plain\expndtw2 \i \f20 \fs22 et al.\i0 , 1998). Serta dilanjutkan dengan Uji
\plain\expndtw1 \f20 \fs22 Tukey HSD dilakukan setelah mendapatkan
\plain\expndtw-4 \f20 \fs22 hasil analisa varian.
\par
\column
\plain\pard\sl-389 \li0 \ri21 \expndtw3 \f20 \fs22 salinitas 20 ppt (37,32 x 10\
up6 \fs13 6\up0 \fs22 sel/ml) dan\fs13 6
\plain\par
\pard\sl-118 \li0 \ri83 \expndtw2 \f20 \fs22 terendah pada salinitas 30 ppt (18,
62 x 10
\plain\par
\pard\sl-252 \li0 \ri22 \expndtw2 \f20 \fs22 sel/ml). Kepadatan\i Nitzschia\i0
sp. mencapai
\plain\expndtw0 \f20 \fs22 puncak pada fase eksponensial yaitu hari ke-5
\plain\expndtw4 \f20 \fs22 sedangkan pada hari ke-6 menunjukkan
\plain\expndtw0 \f20 \fs22 penurunan kepadatan populasi. Hal ini sesuai
\plain\expndtw1 \f20 \fs22 dengan pertumbuhan kultur mikroalga pada
\plain\expndtw0 \f20 \fs22 skala laboratorium umumnya mencapai masa
\plain\expndtw1 \f20 \fs22 panen pada hari ke 7 - 10 (Kawaroe, 2007).
\plain\par
\f20 \fs24 31
\par
{\shp
ffd8ffe000104a46494600010100000100010000ffdb004300010101010101010101010101010101
01010101010101010101010101010101010101010101010101010101010101010101010101010101
010101010101010101ffdb0043010101010101010101010101010101010101010101010101010101
0101010101010101010101010101010101010101010101010101010101010101010101010101ffc0
00110806da04d803012200021101031101ffc4001f00000105010101010101000000000000000001
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}}}
\plain\pard\sl-268 \li11 \ri3 \expndtw1 \f20 \fs22 Pada salinitas 20 ppt mikroal
ga\i Nitzschia\i0 sp.
\plain\par
\pard\sl-251 \li11 \ri0 \expndtw2 \f20 \fs22 mengalami pertumbuhan yang lebih ce
pat
\plain\expndtw-2 \f20 \fs22 dibandingkan dengan salinitas yang lain. Hal ini
\plain\expndtw2 \f20 \fs22 menunjukan bahwa pada salinitas tersebut
\plain\expndtw-3 \i \f20 \fs22 Nitzschia\i0 sp. tumbuh dengan baik.
\plain\par
\sl-242\fs24\par\pard\pard\sl-251 \li10 \ri1 \fi720 \expndtw5 \f20 \fs22 Pada me
dia bersalinitas 20 ppt
\plain\par
\pard\sl-251 \li10 \ri3 \expndtw0 \f20 \fs22 menunjukkan jumlah kepadatan sel ya
ng tinggi
\plain\expndtw4 \f20 \fs22 sedangkan pada salinitas 35 ppt jumlah
\plain\expndtw2 \f20 \fs22 kepadatannya rendah. Hal ini menunjukan
\plain\expndtw0 \f20 \fs22 bahwa pada salinitas yang lebih tinggi terdapat
\plain\expndtw3 \f20 \fs22 hambatan dalam proses pertumbuhan dan
\plain\expndtw0 \f20 \fs22 reproduksi\i Nitzschia\i0 sp. Vazquez-Duhalt dan
\plain\expndtw-2 \f20 \fs22 Arredondo-Vega (1991) melaporkan penurunan
\plain\expndtw5 \f20 \fs22 kadar protein dan biomassa sementara
\plain\expndtw5 \f20 \fs22 karbohidrat dan lipid tidak mengalami
\plain\expndtw2 \f20 \fs22 perubahan selama adaptasi yang dilakukan
\plain\expndtw3 \i \f20 \fs22 Botryococcus braunii.\i0 Hart\i et al.\i0 (1991
)
\plain\expndtw1 \f20 \fs22 menunjukkan penurunan pertumbuhan pada
\plain\expndtw1 \f20 \fs22 salinitas yang lebih tinggi yang disebabkan
\plain\expndtw-3 \f20 \fs22 karena menurunnya proses fotosintesis. Naiknya
\plain\expndtw0 \f20 \fs22 salinitas akan menghambat proses fotosintesis
\plain\expndtw0 \f20 \fs22 (Mironyuk dan Einer, 1986), proses respirasi
\plain\expndtw1 \f20 \fs22 serta menghambat pembentukan sel anakan
\plain\expndtw0 \f20 \fs22 (Soeder & Stengel, 1974). Hal ini merupakan
\par
\column
\plain\pard\sl-267 \li0 \ri23 \expndtw2 \f20 \fs22 bentuk adaptasi yang dilakuka
n organisme
\plain\par
\pard\sl-251 \li0 \ri21 \expndtw-1 \f20 \fs22 terhadap salinitas yang lebih ting
gi. Begitu juga
\plain\expndtw0 \f20 \fs22 dengan\i Nitzschia\i0 sp. yang hidup pada kondisi
\plain\expndtw1 \f20 \fs22 yang berbeda dengan habitat asalnya, maka
\plain\expndtw2 \f20 \fs22 akan melakukan proses adaptasi untuk bisa
\plain\expndtw-5 \f20 \fs22 bertahan hidup.
\plain\par
\pard\sl-251 \li0 \ri22 \fi720 \expndtw1 \f20 \fs22 Salinitas merupakan salah sa
tu sifat
\plain\par
\pard\sl-254 \li0 \ri21 \expndtw0 \f20 \fs22 kimia air yang secara langsung maup
un tidak
\plain\expndtw1 \f20 \fs22 langsung dapat mempengaruhi pertumbuhan
\plain\expndtw2 \f20 \fs22 dan kehidupan organisme air.\i Nitzschia\i0 sp\i .
\plain\expndtw-1 \f20 \fs22 dapat tumbuh pada salinitas antara 0-35 ppt dan
\plain\expndtw3 \f20 \fs22 tumbuh optimal pada salinitas 20-25 ppt
\plain\expndtw3 \f20 \fs22 (Sudjiharno, 2002). Kemampuan masing\plain\expndtw0 \f20 \fs22 masing mikroalga dalam melakukan adaptasi
\plain\expndtw0 \f20 \fs22 berbeda-beda tergantung jenis dan perubahan
\plain\expndtw1 \f20 \fs22 salinitas dari habitat asalnya. Semakin tinggi
\plain\expndtw0 \f20 \fs22 perbedaan salinitas dengan habitat asal maka
\plain\expndtw3 \f20 \fs22 adaptasi yang dilakukan mikroalga akan
\plain\expndtw0 \f20 \fs22 semakin berat begitu pula sebaliknya. Akibat
\plain\expndtw1 \f20 \fs22 dari proses adaptasi yang berat yaitu proses
\plain\expndtw-2 \f20 \fs22 pertumbuhan dan reproduksi mikroalga tersebut
\plain\expndtw-8 \f20 \fs22 terganggu.
\par
\sect\sectd\sbknone\plain\sl-263\fs26\par\pard\pard\sl-243 \li387 \ri992 \expndt
w-2 \b \f20 \fs22 Tabel 2.\b0 Kepadatan rata-rata\i Nitzschia\i0 sp. Pada Ber
bagai Tingkat Salinitas (x10\up6 \fs13 6\up0 \fs22 sel/mL)
\par
\plain\pard\sl-263 \li424 \ri3419 \expndtw-16 \f20 \fs22 Hari
\plain\tx4251 \tab \expndtw-5 \f20 \fs22 Salinitas (ppt)
\plain\par
\pard\sl-266 \li515 \ri1045 \expndtw-26 \f20 \fs22 ke
\plain\tx1602 \tab \expndtw-25 \b \f20 \fs22 15
\plain\par
\plain\tx1067 \tab \expndtw-7 \f20 \fs22 4,50 0,00
\plain\par
\plain\par
\plain\tx1067
\plain\tx2574
\plain\tx4172
\plain\tx5768
\plain\tx7346
\tab
\tab
\tab
\tab
\tab
\expndtw-6
\expndtw-6
\expndtw-6
\expndtw-6
\expndtw-7
\f20
\f20
\f20
\f20
\f20
\fs22
\fs22
\fs22
\fs22
\fs22
5,98 0,163
11,72 1,657
14,47 4,633
7,33 0,493
9,7 0,181
\par
\plain\pard\sl-331 \li563 \ri648 \expndtw-2 \f20 \fs22 3
\plain\tx1067 \tab \expndtw1 \f20 \fs22 11,91 1,339 13,85 1,453
\plain\expndtw-2 \f20 \fs22 4
\plain\tx1067 \tab \expndtw1 \f20 \fs22 27,13 1,324 36,05 1,444
\par
\plain\sl-280\fs28\par\pard\pard\sl-220 \li563 \ri648 \expndtw-2 \f20
\plain\tx2574 \tab \expndtw-6 \b \f20 \fs22 37,32 1,156
\plain\par
\sl-276\fs28\par\pard\pard\sl-220 \li563 \ri747 \expndtw-2 \f20 \fs22
\plain\tx5768 \tab \expndtw-6 \b \f20 \fs22 18,62 1,054
\plain\par
\plain\par
\plain\tx7346 \tab \expndtw-6 \f20 \fs22 19,07 0862
\fs22 5
\par
\plain\sl-297\fs30\par\sl-240\fs24\par\pard\pard\sl-220 \li731 \ri21 \expndtw2 \
f20 \fs22 Hal ini dapat dipahami bahwa naik
\plain\tx5005 \tab \expndtw-8 \f20 \fs22 salinitas
\plain\tx6066 \tab \expndtw-13 \f20 \fs22 dalam
\plain\tx6940 \tab \expndtw-12 \f20 \fs22 suatu
\plain\tx7744 \tab \expndtw-8 \f20 \fs22 perairan
\plain\tx8784 \tab \expndtw-13 \f20 \fs22 dapat
\par
\plain\pard\sl-254 \li11 \ri20 \expndtw-3 \f20 \fs22 turunnya salinitas berpenga
ruh terhadap tekanan
\plain\tx4991 \tab \expndtw0 \f20 \fs22 mempengaruhi organisme melalui perubahan
\plain\expndtw2 \f20 \fs22 osmose

\plain\tx4991 \tab \expndtw3 \f20
.
\plain\expndtw1 \f20 \fs22 secara
\plain\tx4991 \tab \expndtw2 \f20
\par
\plain\pard\sl-251
\plain\tx1518 \tab
\plain\tx2325 \tab
\plain\tx3530 \tab
\plain\tx4991 \tab
engan
dan mekanisme osmoregulasi yang

\fs22 berat jenis air laut dan tekanan osmotik
langsung akan mempengaruhi proses
\fs22 Pengaturan osmose cairan bertujuan untuk
\li11 \ri20 \expndtw-7 \f20 \fs22 metabolisme

\expndtw-16 \f20 \fs22 yang
\expndtw-8 \f20 \fs22 berakibat
\expndtw-9 \f20 \fs22 terhadap
\expndtw-3 \f20 \fs22 menyamakan konsentrasi garam internal d
\par
\plain\pard\sl-251 \li11 \ri23 \expndtw2 \f20 \fs22 penurunan pertumbuhan popula
si. Variasi
\plain\tx4991 \tab \expndtw1 \f20 \fs22 konsentrasi garam lingkungan sekelilingn
ya.
\par
\plain\sl-155\fs16\par\sl-240\fs24\par\par\par\par\pard\pard\sl-240 \li9042 \ri2
1 \expndtw-27 \f20 \fs24 32
\par
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}}}
\plain\pard\sl-411 \li388 \ri236 \expndtw1 \b \f20 \fs22 Gambar 1\b0 . Kepadatan
rata-rata\i Nitzschia\i0 sp. (sel/mL) selama masa pengamatan terhadap
\plain\par
\pard\sl-251 \li388 \ri5064 \fi1277 \expndtw-4 \f20 \fs22 salinitas yang berbeda
.
\par
\plain\sl-256\fs26\par\pard\pard\sl-254 \li11 \ri1 \expndtw0 \b \f20 \fs22 Penga
ruh Salinitas Terhadap Kadar Total
\plain\expndtw-4 \b \f20 \fs22 Lipid\i Nitzschia\i0 sp.
\plain\par
\pard\sl-247 \li11 \ri0 \fi720 \expndtw0 \f20 \fs22 Hasil pengamatan terhadap ka
dar total
\plain\par
\pard\sl-251 \li11 \ri0 \expndtw0 \f20 \fs22 lipid\i Nitzschia\i0 sp. dengan p
erlakuan perbedaan
\plain\expndtw0 \f20 \fs22 salinitas menunjukkan bahwa nilai kadar total

\plain\expndtw1 \f20 \fs22 lipid terbesar terdapat pada salinitas 35 ppt
\plain\expndtw1 \f20 \fs22 dengan persentase rata-rata 71,51%-dw dan
\plain\expndtw5 \f20 \fs22 terendah pada salinitas 15 ppt dengan
\plain\expndtw2 \f20 \fs22 persentase rata-rata 13,26%-dw. Hasil ini
\plain\expndtw-1 \f20 \fs22 menunjukkan bahwa\i Nitzschia\i0 sp. mengandung
\par
\column
\plain\sl-251\fs25\par\pard\pard\sl-254 \li0 \ri20 \expndtw1 \f20 \fs22 lipid da
lam jumlah besar pada saat salinitas
\plain\expndtw-1 \f20 \fs22 meningkat atau dapat juga dikatakan persentase
\plain\expndtw6 \f20 \fs22 kandungan lipid naik seiring dengan
\plain\expndtw0 \f20 \fs22 meningkatnya salinitas (Douglas\i et al\i0 ., 2004;
\plain\expndtw2 \f20 \fs22 Vazquez-Duhalt\i et al\i0 ., 1991a; Hook\i et al\i0
.,
\plain\expndtw1 \f20 \fs22 1995; Hanhua & Kunshan, 2006; Rao\i et al\i0 .,
\plain\expndtw4 \f20 \fs22 2007). Hasil penelitian kadar total lipid
\plain\expndtw2 \f20 \fs22 menunjukkan perbedaan yang sangat nyata
\plain\expndtw-4 \f20 \fs22 dengan perubahan salinitas.
\par
\sect\sectd\sbknone\plain\sl-254\fs25\par\pard\pard\sl-251 \li388 \ri236 \expndt
w0 \b \f20 \fs22 T\b0 abel 2\b .\b0 Kadar Total Lipid yang Dihasilkan\i Nitzsc
hia\i0 sp. pada Berbagai Tingkat Salinitas yang
\plain\par
\pard\sl-251 \li388 \ri6458 \fi1007 \expndtw-10 \f20 \fs22 Berbeda
\plain\par
\pard\sl-269 \li388 \ri1242 \fi1955 \expndtw-3 \b \f20 \fs22 Kadar total lipid (
%-dw) pada tiap-tiap perlakuan salinitas
\par
\plain\sl-223\fs22\par\pard\pard\sl-220 \li388 \ri1329 \expndtw-10 \b \f20 \fs22
Ulangan
\plain\tx1916 \tab \expndtw-11 \b \f20 \fs22 15 ppt
\plain\par
\pard\sl-429 \li388 \ri1416 \expndtw-2 \b \f20 \fs22 1
\plain\tx1916 \tab \expndtw-13 \f20 \fs22 12,94
\plain\par
\plain\par
\plain\par
\pard\sl-427 \li388 \ri1416 \expndtw-6 \b \f20 \fs22 Rata-rata SD
\plain\par
\pard\sl-249 \li388 \ri1356 \fi1528 \expndtw-11 \f20 \fs22 0.47
\par
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}}}
\plain\pard\sl-398 \li387 \ri738 \expndtw-2 \b \f20 \fs22 G\b0 ambar 2\b .\b0 R
ata-rata Kadar Total Lipid\i Nitzschia\i0 sp. Pada Berbagai Salinitas yang Ber
beda
\par
\plain\sl-235\fs23\par\pard\pard\sl-290 \li11 \ri22 \fi720 \expndtw0 \f20 \fs22
Salinitas merupakan salah satu faktor
\plain\tx4991 \tab \expndtw1 \f20 \fs22 lebih besar dari F\dn4 \fs13 tabel\dn0
(4;10;5%)\fs22 = 4,07 atau \expndtw-14{\field{\*\fldinst SYMBOL 97 \\f Symbol }
} <
\plain\par
\pard\sl-235 \li11 \ri23 \expndtw2 \f20 \fs22 yang mempunyai pengaruh besar terh
adap
\plain\tx4991 \tab \expndtw1 \f20 \fs22 0,05 dengan dB (4,10). Hal ini menunjukk
an
\par
\plain\pard\sl-251 \li11 \ri20 \expndtw0 \f20 \fs22 pertumbuhan dan kadar lipid\
i Nitzschia\i0 sp. (Al\plain\tx5005 \tab \expndtw-13 \f20 \fs22 bahwa
\plain\tx5956 \tab \expndtw-8 \f20 \fs22 salinitas
\plain\tx7053 \tab \expndtw-7 \f20 \fs22 berpengaruh
\plain\tx8506 \tab \expndtw-9 \f20 \fs22 terhadap
\par
\plain\pard\sl-254 \li11 \ri4 \expndtw0 \f20 \fs22 Hasan\i et al\i0 ., 1990 ; R
enaud and Parry, 1994).
\plain\expndtw0 \f20 \fs22 Kondisi salinitas yang optimum sangat penting
\plain\expndtw-2 \f20 \fs22 untuk mendapatkan nilai produktifitas\i Nitzschia
\plain\expndtw1 \f20 \fs22 sp. yang tinggi. Pada salinitas tinggi nutrien
\plain\expndtw0 \f20 \fs22 digunakan untuk tumbuh tetapi tidak optimum
\plain\expndtw2 \f20 \fs22 hal ini dikarenakan pada salinitas tersebut
\plain\expndtw3 \i \f20 \fs22 Nitzschia\i0 sp. melakukan adaptasi dengan
\plain\expndtw3 \f20 \fs22 melakukan proses osmose sehingga tidak
\plain\expndtw3 \f20 \fs22 banyak mengeluarkan energi, dan energi
\plain\expndtw1 \f20 \fs22 tersebut tersimpan. Proses adaptasi tersebut
\plain\expndtw0 \f20 \fs22 bertujuan untuk mempertahankan hidup karena
\plain\expndtw2 \f20 \fs22 berada pada lingkungan yang ekstrim atau
\plain\expndtw1 \f20 \fs22 diluar salinitas yang optimal untuk tumbuh,
\plain\expndtw0 \f20 \fs22 adaptasi tersebut dilakukan dengan cara energi
\plain\expndtw0 \f20 \fs22 yang dihasilkan ini digunakan untuk bertahan
\plain\expndtw-1 \f20 \fs22 hidup sehingga pertumbuhan cenderung lambat
\plain\expndtw1 \f20 \fs22 dan energi tersebut tersimpan dalam jumlah
\plain\expndtw4 \f20 \fs22 besar. Sedangkan pada salinitas 20 ppt
\plain\expndtw0 \f20 \fs22 merupakan salinitas optimal sehingga diduga
\plain\expndtw-2 \f20 \fs22 dapat melakukan adaptasi dengan baik terhadap
\plain\expndtw0 \f20 \fs22 salinitas dengan energi yang digunakan untuk
\plain\expndtw0 \f20 \fs22 tumbuh sehingga pada proses pertumbuhannya
\plain\expndtw0 \f20 \fs22 mengeluarkan banyak energi. Disini juga dapat
\plain\expndtw3 \f20 \fs22 dilihat bahwa salinitas secara signifikan
\plain\expndtw0 \f20 \fs22 memberikan pengaruh terhadap pertumbuhan
\plain\expndtw-1 \f20 \fs22 dan kadar lipid mikroalga. Dengan peningkatan
\plain\expndtw0 \f20 \fs22 salinitas dapat menstimulasi kadar lipid yang
\plain\expndtw0 \f20 \fs22 dihasilkan mikroalga (Vazquez-Duhalt\i et al\i0 .,
\plain\expndtw0 \f20 \fs22 1991b). Hasil analisis varian kadar total lipid
\plain\par
\pard\sl-267 \li11 \ri6 \expndtw0 \i \f20 \fs22 Nitzschia\i0 sp. menunjukkan ba
hwa F\dn4 \fs13 hit\dn0 \fs22 = 5,675
\par
\column
\plain\pard\sl-253 \li0 \ri22 \expndtw0 \f20 \fs22 kandungan lipid\i Nitzschia\
i0 sp. Berdasarkan uji
\plain\expndtw0 \f20 \fs22 lanjut menggunakan uji Tukey HSD, diketahui
\plain\expndtw1 \f20 \fs22 bahwa perbedaan terjadi antara perlakuan 15
\plain\expndtw2 \f20 \fs22 dengan perlakuan 30, perlakuan 15 dengan
\plain\expndtw1 \f20 \fs22 perlakuan 35. Pada perlakuan 35 ppt dengan
\plain\expndtw1 \f20 \fs22 perlakuan 15 ppt, 20 ppt, 25 ppt dan 30 ppt
\plain\expndtw-3 \f20 \fs22 menunjukkan perbedaan sangat nyata.
\plain\par
\pard\sl-253 \li0 \ri23 \fi708 \expndtw1 \f20 \fs22 Hasil penelitian menunjukkan
bahwa
\plain\par
\pard\sl-254 \li0 \ri21 \expndtw5 \f20 \fs22 perlakuan salinitas pada media kult
ur
\plain\expndtw-2 \f20 \fs22 berpengaruh terhadap kadar total lipid\i Nitzschia
\plain\expndtw0 \f20 \fs22 sp. Salinitas merupakan parameter lingkungan
\plain\expndtw0 \f20 \fs22 yang berperan penting dalam pertumbuhan dan
\plain\expndtw0 \f20 \fs22 metabolisme\i Nitzschia\i0 sp. Salinitas diperoleh
\plain\expndtw3 \f20 \fs22 dengan memodifikasi lingkungan media,
\plain\expndtw3 \f20 \fs22 salinitas juga berperan dalam mekanisme
\plain\expndtw1 \f20 \fs22 osmoregulasi melalui tekanan osmotik yang
\plain\expndtw1 \f20 \fs22 dilakukan\i Nitzschia\i0 sp. Hasil penelitian juga
\plain\expndtw3 \f20 \fs22 menunjukkan pentingnya dalam uji coba
\plain\expndtw0 \f20 \fs22 perlakuan salinitas pada media kultur\i Nitzschia
\plain\expndtw2 \f20 \fs22 sp. untuk mendapatkan lipid dalam jumlah
\plain\expndtw-11 \f20 \fs22 besar.
\plain\par
\sl-275\fs27\par\pard\pard\sl-220 \li0 \ri2844 \expndtw-7 \b \f20 \fs22 Kesimpul
an
\plain\par
\pard\sl-367 \li0 \ri21 \expndtw1 \f20 \fs22 Perbedaan kadar salinitas pada medi
a kultur
\plain\par
\pard\sl-254 \li0 \ri21 \expndtw2 \f20 \fs22 memberikan pengaruh yang nyata terh
adap
\plain\expndtw3 \f20 \fs22 kandungan total lipid dan kepadatan dari
\plain\expndtw1 \i \f20 \fs22 Nitzschia sp.\i0 Kandungan total lipid terbesar
\plain\expndtw-2 \f20 \fs22 ditemukan pada\i Nitzschia sp\i0 yang dikultur pad
a
\plain\expndtw2 \f20 \fs22 media dengan salinitas media kultur 35 ppt
\plain\par
\sl-142\fs14\par\sl-240\fs24\par\par\pard\pard\sl-240 \li0 \ri239 \fi3794 \expnd
tw-27 \f20 \fs24 34
\par
{\shp
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01010101010101010101010101010101010101010101010101010101010101010101010101010101
010101010101010101ffdb0043010101010101010101010101010101010101010101010101010101
0101010101010101010101010101010101010101010101010101010101010101010101010101ffc0
00110806da04d803012200021101031101ffc4001f00000105010101010101000000000000000001
02030405060708090a0bffc400b5100002010303020403050504040000017d010203000411051221
31410613516107227114328191a1082342b1c11552d1f02433627282090a161718191a2526272829
2a3435363738393a434445464748494a535455565758595a636465666768696a737475767778797a
0101010101010101010000000000000102030405060708090a0bffc400b511000201020404030407
05040400010277000102031104052131061241510761711322328108144291a1b1c109233352f015
6272d10a162434e125f11718191a262728292a35363738393a434445464748494a53545556575859
5a636465666768696a737475767778797a82838485868788898a92939495969798999aa2a3a4a5a6
0028a28a0028a28a0028a28a0028a28a0028a28a0028a28a00ffd9
}}}
\plain\pard\sl-268 \li11 \ri21 \expndtw-3 \f20 \fs22 sebesar 71,51% - dw dan ter
endah pada salinitas
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\plain\par
\pard\sl-251 \li11 \ri401 \expndtw3 \f20 \fs22 15 ppt sebesar 13,26 % - dw. Seda
ngkan
\plain\tx5441 \tab \expndtw-3 {\field{\*\fldinst {\b HYPERLINK}{\b "http://www.s
ciencedirect.com/science/journal/07349750"}}
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o3\colw2678
\plain\pard\sl-387 \li11 \ri0 \expndtw2 \f20 \fs22 kepadatan optimum diperoleh p
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\plain\par
\pard\sl-118 \li11 \ri2 \expndtw0 \f20 \fs22 salinitas 20 ppt dengan nilai 37,32
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\plain\par
\pard\sl-251 \li11 \ri0 \expndtw3 \f20 \fs22 pada hari ke 5, sedangkan terendah
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\fs22 sel/mL pada
\plain\expndtw1 \f20 \fs22 hari ke 6. Kadar salinitas pada media kultur
\plain\expndtw0 \f20 \fs22 mempengaruhi kadar total lipid dan kepadatan
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{\shp
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2a3435363738393a434445464748494a535455565758595a636465666768696a737475767778797a
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05040400010277000102031104052131061241510761711322328108144291a1b1c109233352f015
6272d10a162434e125f11718191a262728292a35363738393a434445464748494a53545556575859
5a636465666768696a737475767778797a82838485868788898a92939495969798999aa2a3a4a5a6
}}}
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{\shp
ffd8ffe000104a46494600010100000100010000ffdb004300010101010101010101010101010101
01010101010101010101010101010101010101010101010101010101010101010101010101010101
010101010101010101ffdb0043010101010101010101010101010101010101010101010101010101
0101010101010101010101010101010101010101010101010101010101010101010101010101ffc0
00110806da04d803012200021101031101ffc4001f00000105010101010101000000000000000001
02030405060708090a0bffc400b5100002010303020403050504040000017d010203000411051221
31410613516107227114328191a1082342b1c11552d1f02433627282090a161718191a2526272829
2a3435363738393a434445464748494a535455565758595a636465666768696a737475767778797a
0101010101010101010000000000000102030405060708090a0bffc400b511000201020404030407
05040400010277000102031104052131061241510761711322328108144291a1b1c109233352f015
6272d10a162434e125f11718191a262728292a35363738393a434445464748494a53545556575859
5a636465666768696a737475767778797a82838485868788898a92939495969798999aa2a3a4a5a6
}}}

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}

Kajian Kadar Total Lipid Dan Kepadatan Nitzschia Sp. Yang Dikultur Dengan

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Kajian Kadar Total Lipid Dan Kepadatan Nitzschia Sp. Yang Dikultur Dengan

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{\rtf \ansi\ansicpg1252\deff0

{\fonttbl{\f11\fswiss\fcharset0 Arial Narrow;}{\f10\fswiss\fcharset0 Arial;}{\f1

\plain\expndtw1 \f20 \fs22 memiliki kandungan lipid yang besar untuk

\plain\expndtw0 \f20 \fs22 ditetapkan kondisi lingkungan yang memadai

\li10 \ri31 \expndtw-1 \f20 \fs22 suhu, nutrien dan cahaya ya

\pard\sl-254 \li1240 \ri756 \expndtw-10 \f20 \fs22 Na2EDTA

\li11 \ri20 \expndtw2 \f20 \fs22 (Thiamin HCl) 0,2 mg;

\li11 \ri21 \expndtw-2 \f20 \fs22 10 mg

\plain\expndtw2 \f20 \fs22 osmose

dan mekanisme osmoregulasi yang

\li11 \ri20 \expndtw-7 \f20 \fs22 metabolisme

\plain\expndtw0 \f20 \fs22 salinitas menunjukkan bahwa nilai kadar total

ngelola Field Station Marine

\sl-285\fs28\par\pard\pard\sl-220 \li0 \ri21 \expndtw3 \f20 \fs22 Erlina, A. 200

\plain\expndtw3 \f20 \fs22 Pengembangan Budidaya Air Payau,

\expndtw-14 \f20 \fs22 Alami

005. Algal culturing

\li361 \ri22 \expndtw-5 \f20 \fs22 exopolysaccharide

\li361 \ri23 \expndtw-4 \i \f20 \fs22 Ind. Eng. Chem. Res\i0

{\sp{\sn fRecolorFillAsPicture}{\sv 0}}

\li362 \ri21 \expndtw-1 \f20 \fs22 Nations, US, 32 hlm. (dite

\plain\tx8587 \tab \expndtw-11 \f20 \fs22 Kultur

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