(CELLULOSE CHEMISTRY AND TECHNOLOGY ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF SOME NATURAL, POLYPHENOLS IOANA IGNAT,’ DANA G. RADU, IRINA VOLE,” ANDREEA I, PAG" and “Aurel Vlaicw” University of Arad, Research, Development & Innovation in Technical and Nawural Sciences Insitute, 2, F. Drigoi Sir. 310330, Arad, Romania ‘Gheorghe Asachi” Technical Univesity, Faculty of Chemical Engineering and Environmental Protection, 71 A, Mangeron Blvd, 700080 lai, Romania “University of Agricultura Sciences and Veterinary Medicine, Cluj-Napoce, 35, Mandgtur Sr, 400372 Cluj-Napoca, Romania Received March 6, 2013 “The presen study provides information on separation and identification of natura bioactive compounds from different ‘ese materials With potent appbicaions as anibucerials and plant growb madulaors. Naural exacts were ‘obisined by alcoholic and water extraction from spruce bark, grape seeds, Crataegus monogyna chao) and Asclepias race (milkoced). HDLCDAD analysis revealed that galic acid (1254 m0 g) and catechin (85.52 ma/100g) wor the most abundant ‘compounds in huvthoen and grape seed extract, vale aid (7.9 mp/100 ) was Tound in high concerns ia Sproce bark wood, while mikweed extracts were characterized by the presence of hydroxycinnamic acids and ‘lavonoids. The DPPH assy indicated thatthe highest radical scavenging activity was rested by gape seeds (ECin += 45.75 pg) and huwthora (EC. of Escherichia coll and Preudomonas aeruginosa specs. Furthemmor, the toxicity of the natural exacts as evalated by tei application i germination ests of Phaseolus vulgaris, Te obtained resis demonstrated tha & hgh concentration of polyphenols has an inhibitory effect on plant development, while lower concentrations determine & ‘considerable stimulation of plane elongation ‘Keywords: polyphenols. extraction, characterization, HPLC, radical seavenging, antibacterial activity, seed germination INTRODUCTION Polyphenolic compounds are a wide spread polyphenolic compounds and plant species. toup of secondary metabolites found inal plants Different activities of polyphenolic products can ‘and represent the most desinble phytochemicals ‘duc to their valuble properties and. potential use as additives in food industry, cosmetics, medicine, and agriculture." In plants, polyphenols play an important role in various defense reactions 10. protect. them ‘against abiotic stesses like UV light or biotic stresses, sach as predators and pathogen attacks, being also involved in-plant growth and ‘development eyek, a they have been shown to regulate the transport of polar axins.” The imolatory of inhibitory effect on plant ‘development depends on the concentration of be similar to those of growth hormones (auxins, cytokines) * Polyphenols have been demonstrated 10 act as famioxidants and free radical scavengers. The intrest in the physiological role of bioactive ‘compounds present in. plants. has increased dramatically over the last decade, panicularly in ‘elation to human health, The antioxidant effets ‘of polyphenols are considered to be derived from the scavenging action of their structures on fonygen containing. free radicals, The in vitro fntiovidant cffoctiveness of polyphenols is ‘essentially the result of the ease with which an H Cellulose Chem, Technol. 47 (5-6), 387-399 (2013)