Methodology to growth wild alga in various pH

Put microalgae and

sea-water (1:4)

Put the mixed of

microalgae and sea
water in 400 ml
Beaker Glass (6
glasses)
Give 1 M of Citric
Acid to Beaker
Glass A (7mL) to
make medium
with ph = 3

Give 1 M of Citric
Acid to Beaker
Glass B (5mL) to
make medium
with ph = 4

Give 1 M of Citric
Acid to Beaker
Glass C (3,2 mL) to
make medium
with ph = 5

Give 1 M of Citric
Acid to Beaker
Glass D (1.5 mL)
to make medium
with ph = 6

Put 21 Watt of Lamp

with the distance 3
cm arround (lef,
right, and above the
beaker glass)
Give 1 mL of Walne
Medium to each
glass

Analyze the growth

of alga with
Turbidimetry
(Absorbance and
%T) every 12 hours

Analyze the growth

of alga with
Counting Chamber
(to know how many
cells of algae) every
12 hours

Make Growth Curve

of Wild
Methodology
of microalgae Mutation
Botryococcuss
braunii for each ph
Put microalgae and
sea-water (1:4)

Give 1 M of Citric
Acid to Beaker
Glass D (0.5 mL)
to make medium
with ph = 7

Count How many

cells of microalgae
with counting
chamber

Lighting microalgae
with UV for 90
seconds

Count How many

cells that still alive
after the mutation
process

Mutant (microalgae
that still alive)

Methodology to growth mutant microalgaealga in various pH

Give 1 M of Citric
Acid to Beaker
Glass A (7mL) to
make medium
with ph = 3

Analyze the growth

Give
M of Citric
of
alga1 with
Acid to Beaker
Turbidimetry
Glass B (5mL)
to
(Absorbance
and
make
medium
%T) every 12 hours
with ph = 4

Put
mutant
Put
Give
Put
211
the
Watt
M mixed
of Citric
of of
Lamp
of
microalgae
and
seaMake
Growth
Curve3
Acid
mutant
with to
theBeaker
microalgae
distance
water
(1:4)
of
mutant
Give
ofmL)
Walne
Glass
and
cm 1arround
sea
CmL
(3,2
water
(lef,
into
Botryococcuss
Medium
to each
400
right,
make
mland
medium
Beaker
above
Glass
the
braunii
ph
glass
withbeaker
(6
phfor
glasses)
= each
5glass)

Analyze the growth

ofGive
alga1with
M of Citric
Counting
Chamber
Acid to Beaker
(to
know
how
Glass D (1.5 many
mL)
cells
of algae)
every
to make
medium
12with
hours
ph = 6

Give 1 M of Citric
Acid to Beaker
Glass D (0.5 mL)
to make medium
with ph = 7

How to compare wild and mutant microalgae

Growth Curve of
Growth Curve of
wild microalgae
mutant
Make
Compare
Analyze
a
conclution
which
with
in various pH
microalgae in
during
International
onethe
thatresult
can ofvarious pH
growth
wild and
Journal
optimally
mutant
incomparasion
acid condition