HCG

(Human Chorionic Gonadotropin)

Dwi Amalia
Yuni Sulistiani
Anisa Rahmadini
Denny Agung Nugroho
D3 ANALIS KESEHATAN
STIKES JENDERAL ACHMAD YANI
CIMAHI

TEORITICAL BACKGROUND

HCG (Human Chorionic

Gonadotropin) is a hormone
produced by placenta tissue in
early pregnancy. This hormone
will be released through the
urine.

PURPOSE
To detect the presence of
human
chronic
gonadotropin in urine and
also detects the presence of
pregnancy

PRINCIPLE

The direct pregnancy assay is

based upon the agglutination
reaction between latex
particles coated with anti-hCG
antibodies and hCG present in
the test specimen.

Tools and Material

Lab Coat

Mask

Used to protecting the

body

Used to protect nose

and mouth

Gloves

Used to protect hand from

infection subtance

Slide test

Used to interpretation
the result

microtube

Used to Place the

sample

pippette

It can used to
pippette and
stirring

Lateks reagent

Disinfectant

Used to detect
antigen in sampel

Used to
sterilization
pippette & stirring

Control (-)

Used to standard
control (-) for
sample

Control (+)

Used to standard
control (+) for
sample

Procedure
Bring the reagent and spesimen to room temperature before
use
Place one drop of the negative pregnancy control onto a circle
of the agglutination slide
Using the pippet-stirrers provided, place one drop of the urine
specimen onto the remaining circle of the agglutination slide
Shake and re-suspend the pregnancy latex reagent. Add one
drop to each of the test circles of the agglutination slide
Stir with individual pippete-stirrer and spread mixture over
entire area of the test circle
Gently rock the agglutination test slide for two minutes and
observe the test circles for agglutination. Interpret results at
two minutes. Extended incubation may result in evaporation
and erroneous result

Interpretation of results

Positive result : agglutination occurs

within two minutes
Negative result : agglutination does
not occur within two minutes

RESULT
In urine Mrs. .... Negative
pregnancy because theres not
occur agglutination. This mean
Mrs. ... Is not pregnancy and of
course there is in urine not
contain antigen anti-HCG.

Thank
you