‘As the companies did not participate as expected, it is not sure that the table represents an exhaustive list of the main products that have the potential to be used in Europe as mycotoxin-detoxifying agents in contaminated feed. It should be noted that most of the companies which answered are aware that products belonging to the functional class of detoxifying agents are yet not registered in Europe, 2.4, Collection of information on mycotoxin-detoxifying agents which has been published on the Internet {In parallel with the collection of information from industrial stakeholders, another task consisted in searching on the Internet for information related to mycotoxin-detoxifying agents. This was done by visiting the website of each company listed and searching for information on mycotoxin-detoxifying agents. classified into 2 ‘The name of the product and its description are given in Table 3. The products wer categories: adsorbing agents or biotransforming agents. All information related to the physical (appearance for example) and chemical (chemical composition) properties were retrieved. The table also mentions information about the marketing and regulation of these agents. Jn vitro and/or in vivo studies, as well as targeted mycotoxins, are also mentioned, This table includes about 35 products and some of them are clearly described as mycotoxin detoxifying agents on the companies” websites This review was performed from July to October 2009 based on available information, the table is therefore not considered as exhaustive. The pasa doer fs been poaicn and aoa by Da vo aon) a sears wn Aaa Sof Ren (Eo) No 11200 has boon ara su ecste hy esr) to ona of art sere: etwas te Espen Faed ‘Sei Autaay ana ho uta. The preva’ cocuatisaaiwed carta wiht Uanepreny praca nie he Exfopen os Salty ‘crys aya aco ui are by SA, are ea en onto ase Sek 34‘Table 3: Table gathering information on different mycotoxin-detoxifying agents (Internet Research) Category 7 Studies Product | Csmpany Physico-chemical Comments Tanted ame Description Peeperiies mycotoxins ToxTrap®™* | ABAC ‘Adsorbing agent | © Powder a Aflatoxin, wonwabsech — | Binder based on ZEAT2 plant fibre toxin SORBATOX | Kioiechagil | Adsorbing agent | » While powder Hydrated aluminium - # Acid pl (49.5.4) silicate: E559 Primarily wonvavilcom | Complexed ‘© Miscible with water designed to hydrated ‘© Melting point > bind aflatoxins aluminium 1200°C° Excellent silicate, with activity against Kaolinite, Fusarium feldspartz, quartz, toxins such as ‘carbonaceous 12, DON or material ZEA Aagrabond | Agranco Corp | Adsorbing agent |» Chemical ‘composition: 63.3 SMMESREENSOS J Calcium and Si02, 21.4% A1203, 2 sodium based 3.8% Fe203, 0.3% alumino-silieate K20, 0.2% Mg0, 0.7% Ca, 2.7% © Light grey # pH:9.0 EMBLTOO | Aevgrown | Adsorbing agent | - Status ingrediontsr GRAS [= International status, comply with the | Aflatoxin Ie. Contains natural ‘American Association of ‘moutmorillonite, Feed Control Officials hhumiditied wommaprionanl | vepetable sscom ‘carbons, mannan and fructose ‘oligosaccharides FLO-BOND | AgivTes ‘Adsorbing agent | » Bull-coloured GRAS Tisted Tab and eld pana studies www. © Chemical (Avantaggiato om Calcium ‘composition; 69.109 tal, 2008) Aluminosilicate Si02, 189% A208, 5.89% Fe203, 2.90% AFBI, T-2 MgO, 1.0% K20, toxin, DON, 1.2% C20, 0.70 OTA, FBI, Ti02, 0.5% NaO, ZEA 5.70% 120. FLO-BOND | Agi-Tec ‘Adsorbing agent |» Buff-coloured GRAS Tisted Tab and field PLUS © ples studies wonw.agritects.c | FLO-BOND © Chemical om buttered ‘composition: 62.80% AFBI,T-2 propionic acid Si02, 17.2% ALO, toxin, DON, 5.39% Fe203, 2.60 OTA, FBI, MgO, 0.90% K20,, ZEA. (E0)No 1702002 he tae har van ara ‘ve os aor) eos Ui bythe ator) ri sont art agar ‘Sei Autaay ana ho uta. The preva’ cocuatisaaiwed carta wiht Uanepreny praca nie he Exfopen os Salty ‘crys aya aco ui are by SA, are ea en onto ase Sek 35Category ‘Studies Product | Company Phyaleo-chemic Comments Targeted same Description. Propert mycotoxins 1% a0, 0.60% 1102, 0.4% N20, 5.10% 420 Aarotax | Reronmed “Riborbing agent | © Crystalline srocare ZEA DON swyovagromed.a | Binds mycotoxins i through rain ‘minerals and components of MTB-To0® | Alltech ‘Adsorbing agent | © Lightirown powder |~ Gann, © Partaly soluble in 2004, wwwaltechco | Non-viable dried |” water (Chowdhury n yeast of and Smith, Saccharomyces 2005), (Diaz et cerevisiae a, 2004), (Kogan and Kocher, 2007), (Meissonnier tal, 2008), Gwany etal, 2002), (Swany tal, 2002), (Vegan ets, 2006) Mycosorb® | Alltech ‘dsorbing agent | © Fine pale brown] Available tn Europe Tan | vanaggiato powder [America and Asia Pacific. | etal, 2005), wowvaltechco | Composedot | « insoluble in water | Notavailablein the USA. (Diaz etal, a elucomannan or Canada 2005), (Diaz- molecules Lsno and extracted fom the Smith, 2006), yeast cell wall of Dror, Saccharomyces 2003), cerevisiae, (Pvorska, sodium calcium 200 aluminosilicate (Karaman t and calcium al, 2005), carbonate (Msehini et a, 2008), (mith eta, 2008), (Swamy tal, 2003, (Swamy etal, 2004), (Volkl and Karlovsky, 1998) Aeomive® | Krome Advorbing agent |e Meling poim> | HSCAS Twedimtbe US| Mineral 000°C Code of Federal Producis,Inc | Hydrated Sodium | Appearence:tan to | Regulations (21 CFR Cale pink 582.2729) as an ati wowvazomiteco | Aluminositcate | « Sojbiliy in water< | eaking agent, and n 1% generally recognized as + Unstabie in oid _| safe GRAS) by the FDA Mycofie® | Biomin ‘Adsorbing and [= Mycofix snot avaiable ts | (Avanaggiato Plus bioteansorming {he US and Canada etal, 2005), www-biominet | agent (Dike et at. The Her cones tas es Sodio! SO BSG Cae Gaede 6 AMO) a SSRN wk AGRO Run (Eo)No trazaia Sei Auto ana emonyia ajo Ray nt 96 ee, Ui bythe ator) ri sont art agar esr! cura: used corr wih Be Uaneparcy ple nih the Eafe Pod Sala sere ar ants azote by EFS EFSA eres te rats, wow na poston ar ego me sc 36Category Trades Product | Company Physico-chemieal Comtaats Targeted same Description. Properties mycotoxins 7002); Composed of Dinicke mix of algse and 20026, Plant extrcis and Dinicke tpeciic enzyines 20028, Dinicke 2003) Aftoxin, Fount, OTA, DON, T2 toxin, ZEA Teo! | Diesen “Aisaibng agent] © Colour green Te dows Waroqn- © Chemical 2OKpMT prevemive | Cardona ct al, wnmzdresenco | Hydrated sodium |” compositon 4OKpMT conective | 2009) mmx and alc atuminiumy silicon, aluminosilicate sodium, calcium, AFBI potassium, ron ‘xides Tilox® | Tapentaco Rikorbing and] = 7 . biotransforming sc Syoertie combination toxin inactivating binding silicates and bapolymers Tlisiop® | Tperiaco ] Adsorbing apart] = > Myco Plus . Selected mineral Feocalla —|Tapentaco Adsorbing agent] = 7 . Balanood wuowsimpenttae | Fy biotic based on o.com ‘mannanoligosacch atides and B- lcs extracted from purified yea cll walls {S.cerevisiee Toxfin® | Kamin Barapa Adsorbing agent] Reale eal | Taino and Brand Toxin Sophos cccopt the | vino tale binder Formulation of United States onan ToCKMOL |, Preserve wed |= 7 . ry to prevent (Eo) No 11200 has boon ara su ecste hy esr) to ona of art sere: etwas te Espen Faed 37Category Trades Product | Company Physico-chemieal Comtaats Targeted name Description. Properties mycotoxins Trexst® | ews Aavorbing agen + Powder 5 AFBI inn aneasiLes CaPTORA [Nowe “Rikoibing agent} © White powder Bentonite AF montmorillonite: ESS8 Fre fowing Clinoplolie: £567 | foto rnyeotonn binder Propionic acid; £280 Composition ‘Aramoni propionet: bentonite red montmorillonite, Silics: ESSIa ‘tino propionic ac, propionate, silica Duciek® | Narek Rasorbing agent |» Chonkcal aio, composition: 4 ZEN Organo- N00, aluminosilicate Zeorak® | Nace ‘Adsorbing agent Daned somporitin: agains: ZEA, worm gropoidien | Adeorting 45.50% SiO, 13. Teton, com. fnganoaluminosi | 15%. Al Oy, 1-8 OTA, cate Fes0s, 126% MeO, clopiazonie 0.1-04% C20, 0001- seid, FBI, 05% Na0,” 0.03 Aflatoxing osnKO Calibrnca | ODA ‘Rikorbing agent] = This Product nat Tor eale [Toro and fe in the USA or Canada Highly-refined ‘montmorillonite sorbent mineral wunw.oilri.com vivo testings Aflatoxin Calibrin Z| O1-Da “Adsorbing agent | ~ This Product isnot for sale inthe USA or Canada www oiricom | Highly-tefined Tavira and in vivo testings montmorillonite Zearslenone sorbent mineral Amadeite® | Ohaix “Adsorbing agent | ~ > wrm.olmix.com | Hybrid composite seunwolmis.com | paterial (anoclay) combining activated montmorillonite and seaweed extracts Mycobond | Opiiite ‘Adsorbing agent |= . > swwcontviteca | Silicon and wk luminium in their {E5) No tera sea has boon sare ot erst by ests) nthe cova fw prt spr: Stes to Erp aed ‘Sy fumarate xbore Te went ocoretn panes cngeg wi be renee pn wich he ape Poo Soa ‘iso aos tay corona Soap by EPO EPSA nes fey Putin sn Ne Ses 38Category ‘Studies Product | Company Phyaleo-chemic Comments Targeted same Description Propet mycotoxins oxide forms Zetox Opinite Adsorbing age] ss www optiviteco | Combination of ca Moldard (for ‘mould and yeast control) and Mycobond (for nycotoxin binding capabilites) ADFIMAX | Realdyme | Adsorbing agent Powder Tongni 2005, Tangni 2005, wow tealdyme. | Binder based on ‘Aoutia 2008, om plant bers ‘Aoudia 2009 Myeo-Adi® | Special ‘Adsorbing agent |» Crean-coloured |= Diaectal, Ntrents Fine powder 2008) Hydrated sodiunvealeium Alato luminosiliate OTA, T2-toxin MyeonAd Az | Special Risotbig eget | ‘Cvantaggato Nutiens sta, 2005) Hydrated seas apeciaiane | 204000 ZEA, ssanssilat | iuiosiicat fumorisin, uaa trchothecenes TBIONTTES | Sad-Chenie AG | Absorbing agent | » Beigeany powder —]- and # Pattcle sie: 85-75% FENA® | uy | Surface ative < 63 um MIN somusid: | clayminerals |» pH value: 90-100 stemiscom | (entoite, © Chemical ‘montmorilonite) |” composition: 58.0 Bentonite, Si02, 3.5% Meo, alkaline activated 20,0% AI203, 2.0% Na20, 6.0% F208, 1.076 K20, 2.5% cao © Loss on ignition 7.5 % TOXISORB | Sii-Chomie AG | Aisorbing agent | © Beige-grey powder | No Myeofonin claims are | Taw audies @ Classic «Particle size:min | made in the USA, EU and | using AFBI, smal Partly modified |” 60% < 63 ym Canada OTA, ZEA, T- womusud: | aluminosilicate | pi vaue:9 511.0 2 toxin, shemiecom yt high and © Chemical Fumonisin selective surface composition : 59.0% area oP ‘MgO, Effective Bentonite, 168% A1203, 3.1% against AFBI alkaline acivated | Na20,4 © Loss on ignition 8.0 The jeer soar fs ion pdt an nid Cen Snag ehve os OT) a STN wn GR of Reon (E0)No 1702002 he tae har van ara Sei Autoty aa to utes. ‘Stirred nd te conto reached ni present doce ihe roa oh geo be uh 39 Ui bythe ator) ri sont art agar esr! cura: used corr wih Be Uaneparcy ple nih the Eafe Pod Sala sere ar ants azote by EFS EFSA eres te rats, wow na poston ar ego me scCategory ‘Studies Product | Company Phyaleo-chemic Comments Targeted same Description Propert mycotoxins TOXISORE | Sii-Chenie AG | Adsorbing ageat | © Beige-gney powder | No Mycotoxin caine are | Eifective Premium Panicle size: min | made inthe USA, EU and | against AFBI, i Panay modified |" 60%-<63 um Canada OTA, ZEA, wowusud: | atuminositcate | «pH value:9.5-11.0 T2-toxin, FB: shemiccom | yitnhighand | ¢ Chemical selective surface composition : 57.5% ca $102, 4.0% MgO, Organic modified 16.0% AI203, 2.7% ‘bentonite ‘Na2O, 4.1% Fe203, 0.7% K20, 2% CaO # Loss on ignition 12.0 % FIKAT® | Sid-Chenie AG | Seesied © Beige to grey powder ‘rroqui Aluminosilicate © Grain size: 70% < 63 Cardona et al., wth igh We 2008) binding # pi value: 85— 105 Invitro studies characterises for |” "(go g/11120) ting AFBI, aflatoxins © Chemical OTA, ZA, T- ‘composition: $0-65% 2toxin, SiO. 3-6% MgO, Fumonisin 15-2544 AO, 3-64 Fe,03, 3-6% CaO, NasO/K,0 <5% a ‘Adsorbing agent | © Light cream colour | Sweeties ESS ‘Woschinr et «Fluid powder Sepolite: F562 a, 2008) wwwstolsa.com | Natural SHOAL | Gomination oF Aloo smectite and sepioite of high purity Adsorbing get] - UT-Afiaral iw egisered in Taiwan and many parts Cotains natural of Asa, Latin Ameria, www.ubleorp.ca | montmonilonite North America under » layer silicate private label andr license rmineal clays and, agreement humidified vegetable carbons, mannan and fructose oligosaccharides ZAR-MIN | Teo te “Adsorbing agent |» Offtite colour [FDA approved for wse as | Research © Meting point> | anani-caking agent, | studies are woww.zeoine.co | 100% natural 120°C available on a zeolite the website slinoptioie, zeolite, hydrated Binds large sodium potassium spectrum of calcium mycotoxin aluminosilicate (E0)No 1702002 he tae har van ara Sei Autoty aa to utes. ‘Siren rd te conto reached nie pesent doar mio roa oh geo be 40 ve 0 aon) aoa Ui bythe ator) ri sont art agar esr! cura: used corr wih Be Uaneparcy ple nih the Eafe Pod Sala sere ar ants azote by EFS EFSA eres te rats, wow na poston ar ego me sc‘CONCLUDING REMARKS - Two main categories of feed additives can be defined: - Adsorbing agents: one of the strategies for reducing the exposure to mycotoxins is to decrease their bioavailability by including various mycotoxin adsorbing agents in the compound feed, which leads to a reduction of mycotoxin uptake as well as distribution to the blood and the target organs, - Biotransforming agents: another strategy is the degradation of mycotoxins into non-toxic ‘metabolites by using biotransforming agents such as bacteria/fungi or enzymes. ~ Both review of literature and Internet rescarch are more abundant when dealing with adsorbing agents than with biotransforming agents, = Most of adsorbing agents, especially aluminosilicates, have been tested for their ability to bind aflatoxins, Adsorbing agents such as yeast cell wall, bacteria and others can target a larger spectrum. of mycotoxins. Conceming mycotoxin biotransforming agents, as anticipated by their mode of action, their spectrum in term of targeted mycotoxins, are narrower, The pasa doer fs been poaicn and aoa by Da vo aon) a sears wn Aaa Sof Ren (Eo) No 11200 has boon ara su ecste hy esr) to ona of art sere: etwas te Espen Faed ‘Sei Autaay ana ho uta. The preva’ cocuatisaaiwed carta wiht Uanepreny praca nie he Exfopen os Salty ‘crys aya aco ui are by SA, are ea en onto ase Sek 41