Professional Documents
Culture Documents
Gonsultants, [m*
iames D. McGurre;r
Chief Executive Cilicer
lnvoice
Date
5i
31i20l(r
lnvoice #
2 I (){)4
BillTo
City of Bisnrarck - Public lVorks
Attn: iVIs. LaVonnc E. Wohl
PO Box 5503
P.O. No.
Terms
Projeci
BBPH Building
Net 30
P I 6-0296
Ovcr 6 Samples
4 N,lold/Fungi Analysis - Surthce Sr.r'ab or Tape
Amount
Rate
Description
Quantity
- l -3 Day TA-l'
Total
IOOB Eost Cenlrcl Avenue . Bismorck, ND 585O1
(7011223"7335 n Fox VAll223-734t)
625.00
625,00
60.00
.180.00
-<0.00
2{$.00
$r.io-s.oo
fficn*un*ants,
$rx,.
lames D. McGurren
Cliief Exc;u:ive Oilicer
.lune 6,2016
o
.
.
r
o
r
r
.
r
o
& hallway.
Sample #8 was collected from throughout the first floor, BBPI{ south half offices
& hallway.
Sarnple #9 was collected lrom the first t-loor, BBPH fieight elevator.
Sample #l I was collected from thror.rghout the first floor, Wagner Financial
Services offices.
Sample #12 was collected frorn throughout the second floor, BSC, east third
oflices & halhvays.
'1O08
"
'
Bismorck, ND 58501
o
o
Sample #13 was collected trom throughout the second lloor, BSC. middle third
offices & lrallways.
Sarnple #14 n'as collected from throughout the second tloor, BSC, west third
of{ices & hallways.
Sample #15 was collected from throughout the top tloor, NE elevator shaft
stainvell.
Sample #18 was collected from throughor.rt the first floor, BBPH. Diane's otJ-rce.
Four (4) surtace swab samples lvere also collected for the is project:
r
.
r
o
Sarnple #5 was collected from suspect mold growth on the east rvall of the
basement level. #3 Door storage room.
Sarnple #10 ',vas collected from dust and debds on an air filter, located in the frrst
The air samples were collected utilizing a Buck BioAire bioaerosol sarnpling pump w'ith
Allergenco D Cassettes (spore traps). The Buck-sampling pump is calibrated up to l5
iiters per minute using a pump specific rotameter. Allergenco D cassettes are designed
for rapid collection and analysis of a wide range of airbome particulates, including fungal
spores. Ihe surface swab sarnples were collected utilizing a Copan Venturi Transystem
Transport Swab.
The air and swab samples were submitted to Hayes Microbial Consuiting in Midlothian.
VA (Hayes), where they were analyzed for moldlfungi using a light microscope fitted
with a 100X oil immersion objective for a total magnification of 1000X.
RESULTS
Because mold/fungi is tbund everywhere throughout nature, air samples collected lrom
suspect areas need to be evaluated against samples collected from non-suspeot areas or
outdoors (control sample).
As a general rule of thumb the tirngi found from the indoor sample should match the
control sample and be present at levels equal to or less than those of thE control sample.
Higher levels of fungi indoors or ditBrent fungi genera from the control sample can
indicate a possible indoor source.
The presence of ceftain fungi in indoor envirorunents is signiticant because tungi can and
produce mycotoxins. metabolites that can cause adverse health etfects in humans and
animals. Sylnptorns due to exposure of mycotoxins include dermatitis, cold and t'lu
symptorns. sore throat, headache, fatigue, diarrhea, and impaired or altered immune
function, u,hich may lead to oppo$unistic infbction.
will
Results from all thirteen (13) indoor air samples indicated the total mold spore
concentrations were lower than the outside control sample, with common fungal species
identified.
Results fiom all fbur suriace swab samples indicated No Fun-ei Detected.
SITE OBSERVATIONS
BEC did not observe any water darnage or mold contaminated building materials during
this assessment.
Temperature and relative humidity (RH) readings rvere collected r.vithin the Site utilizing
an Extech Temperature and Relative Humidity meter (Extech). The temperature ranged
fram 72 to 78 degrees Fakenheit and the RH ranged trom 36alr. Ta 42%a.
c oN cr.,u sI o N S/RECOMME_ND ATICN S
Based on our onsite obsen ations and air and srvab sample analysis results, there does not
appear to be a mold/iiingi problern rvithin the Site, at the tirne of this assessment.
BEL- is not responsible tor design of repair and/or renovation activities tbllowing
remedial work or repairs to correct moisture intrusions. Please be advised that BEC
makes no representation or rvarranties regarding the cause of moisture intrusion or tbr
llrther moisture intrusion. rvhether lrom civil or structural, sewer 01'water line, or storm
water defects or events. The Customer wiil hold BEC harmless in the event of f'uture
moisture intrusions and subsequent mold growth and BEC is not responsible for
determining or repairing the cause and ettect of any tuture moishre intrusions.
Please contact us at223-7335
regarding this project.
Sincerely,
rt**e_{:*k**
lv1ark Emter
President
r\tiarchment
HMC #16014242
MICROBIAL CONSULTING
3005 Easl Boundary Terrace, #F
Midlothian, VA 23112, USA
804.562.3435 Fax: 804.447.5562
contact@hayesmicrobial.com
http://hayesmicrobial.com/
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Page
of 10
.?.d
ffi#e-W ffi*e
MTCROBIAL CONSULTING
3005 Easl Boundary Terrace, #F
Midlothian. VA 23112, USA
804.562.3435 Fax: 804.447.5562
HMC #16014.?.42
P16-0296
Public Healih Building
500 E Front Ave
Bismarck, ND
We would like to thank you for trusting Hayes Microbial for your analytical needs. On June 6, 2016 we received 18 samples by FedEx for the job
referenced above. 18 samples were received in good conditionThe results in this analysis pertain only to this job, collected on the stated date and should not be used in the interpretation of any other job. This report
may not be duplicated, except in full, without the written consent of Hayes Microbial Consulting, LLC.
This laboratory bears no responsibility for sample collection activities, analytical method limitations, or your use of the test results. lnterpretation and
use of test reiults are your responsibility. Any reference to health effects or interpretation of mold levels is strictly the opinion of Hayes Microbial
Consulting. ln no event, shall Hayes Microbial Consulting or any of its employees be liable for lost profits or any special, incidental or consequential
damages arising out of your use of the test results.
Page 2 of l0
ffi&Yffiffi
MICROBIAL CONSULTING
3005 East Boundary Terrace, #F
Midlothian, VA 23112, USA
804.562.3435 Fax: 804.447.5562
Job
Number:
by:
Email:
Collected
P16-0296
Name:
Mark Ernter
badlands@midconetwork.com
soP
#HMC101
HMC #16014242
Date Collected: 06f03I2016
Date Received: 0610612016
Date Reported: 06/06t2016
HMC lD Number
Sampte lD#
16014242 - 1
16014242 - 2
16014242 - 3
16014242 - 4
Sample Name
Sample Volume
13 spores/M3
13 spores/M3
Limit of Detection
Backoround Fraqments
Count I M3
Count
Alternaria
75 liters
ND
o/o
Raw
Organism
oI
Total
Count / M3
12
160
4.5o/o
1840
Basidiospores
BipolarislDrechslera
Chaetomium
28
373
51.90k
2.3%
10.5%
Cladosporium
80
1067
30.1%
27
266
3547
BO
ND
o/o
Raw
Count
138
6
Ascospores
Asoerqillusl Fenicillium
Basement Middle Offices Bas, retn West 1/3 Offices Break Rm Mecl Rms
75 lilers
75 liters
13 spores/M3
13 spores/M3
Qf
Tolal
40
27
22.5%
13
40
120
Raw
Count
,t
ND
Count / M3
Yo
of
Toial
13
24.5o/o
10. Bo/o
13
24.50k
33.30/,
27
50.9%
53
33.3o/o
ND
e/o
Raw
Count
Count / M3
ot
Total
27
67.5o/o
13
32.5o/o
40
Curvularia
Epicoccum
Fusarium
Memnoniella
Myxomyceles
Pithomyces
Stachybotrys
Stemphylium
Torula
<
1o/o
Ulocladiurn
Unspecified Spore
Total
f--Ei;
sisnarure:
p.mrssl,ldio.t
r-
-]f-----
_A/rWb,/*"
com,noi Aro,s,n
---lfsrqr'rtrrrishE,
'ho
lf
sisn'!.qfr){
Hirfi
urarl
orkie A,
lfj:'--8ffiffifrftt-:]
Dare:
06/0612016
Page 3 ol
10
ffi&-w'ffiffi
MICROBIAL CONSULTING
3005 East Boundary Terrace, #F
Midlothian, VA 23112, USA
804.562,3435 Fax: 804.447.5562
by:
Email:
Job
Name:
badlands@midconetwork.com
HMC lD Number
Sample lD#
Sample Name
Sample Volume
Limit of Detection
Background \ Fragments
Organism
Alternaria
Ascospores
AspergilluslPenicillium
Basidiospores
BipolarislDrechslera
Chaetomium
Cladosporium
Curvularia
16014242 - 6
6
-7
ol
Total
40
40
37.4%
37.4%
27
25.20/o
Raw
75 liters
13 spores/M3
ND
Count I M3
Count
DateCollected: 06/03/2016
DateReceived: 06/062016
DateReporled: 06/06/2016
$au242-9
16014242- 8
ND
Count / M3
HMC #1601424?.
o/o
Raw
Count
SOP #HMC101
16AM242
tx
ND
ol
Tolal
o/o
Raw
Count / M3
Count
QI
Total
Raw
Counl
ND
Count / M3
to of
Total
13
16.5%
13
16.5%
27
28.7%
40
37.70/i
53
67.1o/o
67
71.30k
53
50.0%
'13
12.30k
Epicoccum
Fusarium
Memnoniella
Myxomycetes
Pithomyces
Sta0hybotry$
Stemphylium
Torula
Ulocladium
Unspecified Spore
Total
107
79
94
106
[:-meffi H$"trg*,:3.,s,---:]Mt*Irlrrrrrjr9i]fmt:ill-sqM
$ignature:
06106/2016
Reviewedby:
06/06/2016
Page 4 of
10
X,frAYffiS
MICROBIAL CONSULTING
3005 East Boundary Terrace, #F
Midlolhian, VA 23112, USA
804.562.3435 Fax: 804.447.5562
by:
Email:
Job
badlands@midconetwork.com
HMC lD Number
Sample lD#
Sample Name
Sample Volume
Limit of Detection
Background \ Fragments
16014242 -
Name:
w*lt-**
11
SOP #HMC1O1
HMC #16014242
DateCollected:
DateReceived:
Date
r0orqr42-1l
Reported:
06103/2016
06/06f2016
06/06/2016
16014242
14
11
12
13
14
Count / M3
Count
Altemaria
Ascospores
13lqotegMl=___*__-ll
13 spores/l\43
2\ND
Raw
Organism
75 liters
13
%ol
Raw
Total
Count
12.3%
il
Count / M3
40
%of
Raw
Total
Count
25.0Yo
13
spores/M3
\
Count
ND
/ M3
13
Yo
ot
Total
1?,30/o
RaW
Count / M3
Count
lo
of
Total
27
25-2o/o
13
12.10k
67
62.6o/o
107
Aspersillusl Penicillium
Basidiospores
BipolarislDrechslera
Chaetornium
Cladosporium
Curvularia
Epicoccum
Fusarium
Memnoniella
Myxomyceles
Pilhomyces
Stachybotrys
$temphylium
Torula
93
87.7%
120
12
160
75.00h
93
106
87.704
Ulocladiurn
Unspecified Spore
Total
l*:*W"t,a' pd"a0lIilidt"'
sisnature:
106
lf*--'T.m*o"
:*kbe&--*-_--*__
ffddil****][_SF1ug!g$Lg9,j1-_* IT
Dare:
06/06,2016
Reviewedby:
s'd;itl"a^try r"'tgl,*r
&2,&*-
tqryjt$iiG At'-l
Dare:
06106/2016
Page
5 of 10
ffi&w'ffi
ffi
MICROBIAL CONSULTING
3005 East Boundary Terrace, #F
Midlothian, VA 23112, USA
804.562.3435 Fax: 804.447.5562
by:
Email:
Job
Name:
badlands@midconetwork.com
HMC lD Number
Sample lD#
$arnple Name
Sample Volume
Limit of Detection
Backqround \ Fragments
16014242- 1s
13 spores/M3
Count / M3
Yo
oI
Raw
Total
Count / M3
Count
40
30.1o/o
13
13
9.8%
27
33.8%
13
16-3o/o
27
33.8o/o
80
10
133
DateReported:
06/06/20'16
70 Ot
80
HMC #16014242
Date Collected: 06/03/2016
Date Received: 06/06/2016
Nt)
Total
16.3%
fHMc101
13 spores/M3
ND
soP
18
1B
Count
Alternaria
Ascospores
AsperqilluslPenicillium
Basidiospores
BioolarislDrechslera
Chaetomium
Cladosporium
Curvularia
Epicoccum
Fusarium
16014242
15
Raw
Organism
60.2%
Memrroniella
Myxomycetes
Pilhomyces
Stachybotrys
Stemphylium
Torula
Ulodadiunr
Unspecified Spore
Total
t-fateiog;ilqo
sisnature:
il'l,t t*"{"^rl*
06/06/2016
Revie'wedby:
lrj- // /Ua*
Date:
06/06/2016
Page 6 of
10
hf;AYHS
MICROBIAL CONSULTING
3005 Easl Boundary Terrace, #F
trlidlothian, VA 23112, USA
804.562.3435 Fax: 804.447.5562
Job
by:
Sample lD Number: 5
Organism | $pore Estimate
Direct lD Analysis
Sample
Modia:
Name:
SOP #HMC102
HMC #16A1d'242
Date
Date
Date
Collected:
Received:
Reported:
06/03/2016
06/06/2016
06/06/2016
Swab
Note
No Fungi Detected
Sample lD Numher; 10
Organism I Spore Estimate
Note
No Fungi Detected
nnvcetiafestimite I
Note
No Fungi Detected
orgnniim I
sp9ry-!st14{9
mycelialEmmatel
Note
No Fungi Detected
Signalure:
06/0612016 Reviewedby:
Page 7 ol
'10
&#&wffiw
MICROBIAL CONSULTING
3005 East Boundary Terrace, #F
Midlolhian, VA 23112, USA
804.562.3435 Fax: 804,447,5562
Limit of
Detection
HMC #16A14242
The Limit of Detection is the lowest number of spores that can be detected based on the total volume of the sample collected and the percentage of the
slide that is counted. At Hayes Microbial, 100% of the slide is read so the LQD is based solely on the total volume. Raw spore counts that, exceed 500
spores will be estimated.
Blanks
Background
The Backgrcund ls he amount ot dbrh 01at b prcBfft h th amde. This debds consirls of slln cells, dirt, dust, pollen, drywall du6i and othor oanic
and non-orgnic mauer. A3 tle baclground deosity incr$ses. fie [kdhood of sporcs, especially small spores such as ulose ol AsperualuE and
Psicillium may be obs{urd. Ths backgrcurd b laied on a scal6 of 1 to 4 and each l6vl is determined as fndlofsi
ND
I
2
3
4
5
Rgcolt.ctsample.
t25%
of ff6ld o.dudd.
2$75% oflield occludd.
:7$80% of tud ocdudsd.
: >90% ot liald .ccludd. Sugsesi rcdledion of sample.
:
Fragments
Fragments are small pieces of fungal mycelium or spores. They are nol identifiable as to type and when present in very large numbers, may indicate
the presence of mold anrplification.
lndoor/Outdoor
Comparisons
There are no national standards for the numbers of fungal spores that may be present in the indoor environment. As a general rule and guideline that is
widely accepted in the indoor air quality field. the numbers and types of spores that are present in the indoor environment should not exceed those that
are present outdoors at any given time. There will always be some mold spores present in "normal" indoor environments. The purpose of sampling and
counting spores is to help determine whether an abnormal condition exists within the indoor environment and il it does, to help pinpoint the area of
contamination. Spore counis should not be used as the sole determining factor of mold contamination. There are many factors that can cause
anomalies in the comparison of indoor and outdoor samples due to the dynamic nature of both of those environments.
@Thesemol<JsarecommonlyseeninconditionsofprolongedWaterintrusionandusuallyindicateaproblem.
@Althoughallrnoldsarepotentialallergens,thesearethemo$tc0mmonaltergensthatmaybefoundindoors.
@ThesporecountisslightlyhigherthantheoutSidecountandmayormaynotindicatea$oUrceofcontamination.
T Sgrlilioa,rtly-Higher than Oirtside Air-] The spore count is significantly higher than the outdoor count and probably indicates a source of contamination.
Ratio
Color Note
The types of spores found indoors should be similar to the ones that were identified in the outdoor sample. Significanl increases (more than
25o/ol in the ratio of a particular spore type may indicate the presence of abnormal levels of mold, even if the total number of spores of that
type is lower rn the indoor environment than il was outdoors.
Fungi that are presert in indoor samples al levels lower than 200 per cubic meter are not color coded on the report, unless they are one of the water
damage indicators.
Page
oi
10
n"fi&Yffis
MICROBIAL CONSULTING
3005 East Boundary Terrace, #F
Midlothian, VA 23112, USA
804.562.3435 Fa* 804,447.5562
Direct lnformation
HMC #16014242
Spore Estimate
ND
Raro
Light
Moderate
Heavy
Very Heavy
None Detected
Oo/o
<10
1-',|.u%
11-25o/o
26-50%
10000 orgreaterspores
5l-1000/0
Mycelial Estimate
ND
None Delected
Trace
Few
Many
Pago
o{ 10
ffi&Hffi$
MICROBIAL CONSULTING
3005 East Boundary Terrace, #F
Midlothian, VA ?3112, USA
804.502.3435 Faxr 804.447.5562
hftt,to
ffi6aM,242
Alternaria
Habitat: Commonly found outdoors in soil and decaying plants. lndoors, it is commonly found on window sills and other horizontal surfaces.
Health Effects: A common allergen and has been associated with hypersensitivity pneurnonitis. Alternaria is capable of producing toxic metabolites which
may be associated with diseaso in humans or animals. Occasionally an agenl of onychomycosis, ulcerated cutaneous infection and chronic
sinusitis, principally in the immunocompromised patient.
Ascospores
Habital: A large group consisting of more than 3000 species of fungi- Common plant pathogens and outdoor numbers become very high following
rain. Most of the genera are indistinguishable by spore trap analysis and are combined on the report.
Health Effects: Health affects are poorly studied, but rnany are likely to be allergenic.
AspergilluslPenicillium
Habitatl The mosl common fungi isolated from the environment. Very cornmon in soil an<l on decaying plant material. Are atrle to grow well indoors
on a wide variety ol subslrates"
Health Effects: This group contains common allergens and many can cause hypersensitivity pneumonitis. They may cause extrinsic asthma, and many are
opportunistic pathogens. Many species produco mycotoxins which may be associated with disease in humans and other animals. Toxin
production is dependent on the species, the food source, competition with other organisms, and other environmental conditions.
Basidiospores
Habitat: A common group of Fungi that includes the mushrooms and bracket fungi. They are saprophytes and plant pathogens. ln wet conditions they
can cause structural damage to buildings.
Health Effects: Common allergens and are also associated with hypersensiLivity pneumonitis.
Cladosporium
Habitat: One of the most common genera worldwide. Found in soil and plant debris and on the leaf surfaces of living plants. Ihe outdoor numbers
are lower in the winter and often relatively high in the summer, especially in high humidity. The outdoor numbers often spike in lhe late
afternoon and evening. lndoors, it can be found growing on texliles, wood, sheetrock, moist window sills and in HVAC supply ducts.
Health Effects: A common allergen, producing more than 10 allergenic antigens and a common cause of hypersensitivity pneumonitis.
Myxomycetes
Habitat: Found on decaying plant material and as a plant pathogen.
Health Effects: Some allergenic properties reported, but generally pose no health concerns to humans.
Page 10 of
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