LIST OF TABLES

Table

Title

Page

1.1

Taxonomic Classification of A. annua

2.1

Chemical Composition of Seed Germination Medium and

29

Tissue Culturing Medium

2.2

Chemical Composition of Media used for Transformation

32

2.3

Composition of Solutions used in Molecular Analysis

36

2.4

PCR Program for Amplification of rol B Gene

37

2.5

PCR Program for Amplification of rol C Gene

37

2.6

Chemical Composition of Reagents used in the Antioxidant

44

Assays and Phytochemical Analysis

2.7

Standards for Phytochemical Analysis using HPLC with

Corresponding Wavelengths

46

LIST OF FIGURES
Figure

Title

Page

1.1

Artemisia annua

1.2

Chemical Structure of Artemisinin and its Derivatives

1.3

Molecular Structure of DPPH

22

1.4

Schematic Representation of Chemically Stable Radical

Scavenging Activity using DPPH as Stable Free Radical

22

2.1

Schematic Diagram of the T-DNA Region of the Plasmid of

GV3101 Strain

33

3.1

In vitro Grown Seedlings of Hyb1209r and Hyb8001r on MS

Medium

49

3.2

Regeneration Response of Artemisia annua Explants on

Selection Medium

50

3.3

Regeneration Efficiency of Explants on Selection Medium

51

3.4

Shooting of Hyb1209r and Hyb8001r from Nodal Explants on

SIM Medium

52

3.5

Shooting of Transformants from Nodal Explant

53

3.6

Polymerase Chain Reaction showing Amplified Products of

rol B and rol C in Transgenic Hyb1209r (Shennong)

55

LIST OF FIGURES
Figure

Title

Page

3.7

Polymerase Chain Reaction showing Amplified Products of

rol B and rol C in Transgenic Hyb8001r (Zenith)

56

3.8

Semiquantitative RT-PCR of Transgenic Lines of Hyb1209r

(Shennong)

58

3.9

Semiquantitative RT-PCR of Transgenic Lines of Hyb8001r

(Zenith)

59

3.10

IC50 Value for DPPH Scavenging Activity of Transformed and

Untransformed Plants of Hyb1209r (Shennong) and Hyb8001r
(Zenith)

61

3.11

TRP (Total Reducing Power mg AAE/g Dry Extract) of

Transformed and Untransformed Plants of Hyb1209r
(Shennong) and Hyb8001r (Zenith)

63

3.12

TFC (Total Flavonoid Content mg QE/g Dry Extract) of

Transformed and Untransformed Plants of Hyb1209r
(Shennong) and Hyb8001r (Zenith)

64

3.13

TPC (Total Phenolic Content mg GAE/g Dry Extract) of

Transformed and Untransformed Plants of Hyb1209r
(Shennong) and Hyb8001r (Zenith)

66

3.14

HPLC Analysis of Some Important Phytochemicals (mg/g Dry

Extract) of Transformed and Untransformed Plants of
Hyb1209r (Shennong) and Hyb8001 (Zenith)

68

3.15

Artemisnin Content (mg/g Dry Extract) of Transformed and

Untransformed Plants of Hyb1209r (Shennong) and Hyb8001r
(Zenith)

70