Cetylpyridinium

Mouthwash
Antioxidants
in Edible Oils in
Using
AccucoreUsing
Polar Premium
Acclaim Surfactant Plus with Conductivity Detection
300

200

mAU

3
1

mAU

7
4 6

10

9
11 12

Conductivity

00
00

UV 220 nm

2 5

4 10 6
Minutes

Minutes

Peaks: 1. Propyl Gallate

+2. Ethoxyquin
CI
N3. THBP

4. TBHQ

5. NDGA

6. Ionox-100

15 8

Column: ThermoThermo
Scientific
Column:
Scientific
AcclaimAccucore
Surfactant Plus
3.0 m,Polar
Dimensions:
3.0 Premium
150 mm

Dimensions: Thermo2.6
m, 3.0 Dionex
100 mm
System:
Scientific
Mobile Phases:
A: IC
Methanol
ICS-3000
system : Acetonitrile : Acetic Acid

50:50:0.5 (v/v/v)
Mobile
Phases: A: Acetonitrile
B: 100 mM
B: 0.5%
Acetic
Formic
acid acid in water
Gradient times:
C: Water-4 0 5 10
%A -12
25
Gradient times:
0 25
12 100 20 100
75
%A %B 15
15 75
40 0 40 0
5 5 5
%B curve 5
5 4 5 5
0.80
%CFlow: 80
80 mL/min
55
55

30 C
Flow Temperature:
rate:
0.500
mL/min
Injection: 5 L 2 L
Injection:
A
Detector: 25 C Diode array spectra 250 400 nm,
Temperature:
UV 220 UV
shown
Detection:
nm280
withnm
blank
subtraction;
B
Sample Prep:
Liquid-Liquid
Extraction
Conductivity
with blank
subtraction

Thermo(See
AOAC 983.15);
g sample

Suppressor:
Scientific
Dionex 5.5
CSRS
300, 2tomm
C
(Externalmake
mLmL/min;
of extract
water101.0
current 8 mA)

Samples:

A. 12 antioxidants, 50 ppm each in

C. Butter oil
D. Margarine, preserved with TBHQ

Peaks:
1. Sodium acetonitrile
2. Cetylpyridinium
B. Olive oil

2010

7. BHA
8. Octyl Gallate
9. BHT
10. Lauryl Gallate
11. Vitamin E Acetate CH3
12. Vitamin E

PB20627_E 10/12S
PB20875_E 10/13S

Alkyl pyridinium
salts are
antibacterial
agents
used in various
Phenolic
antioxidants
are mild
commonly
used as
preservatives
for
pharmaceutical
products
such rancidity.
as mouthwash,
drops,
and
edible
oils and fats
to prevent
AOAC cough
Method
983.15
was
so forth. The
Surfactant
Plus food
column
conveniently
separates
developed
to Acclaim
assay levels
in finished
products.
This method
cetylpyridinium
good
peak
shape,suitable
and suppressed
specifies
a C18 with
column
that
provides
selectivityconductivity
in many
provides
interference-free
detection.
cases,
butsensitive,
sometimes
there can be matrix
interferences; for example
propyl gallate coelutes with the unknown matrix peak. The Accucore
Polar Premium, based on solid core technology, provides the speed,
efficiency, and alternate selectivity to resolve propyl gallate from the
unknown matrix peak.

2012 Thermo Fisher Scientific. All rights reserved.

2013 Thermo Fisher Scientific Inc. All rights reserved.