Lecture 5 Bacterial Spores

Dr. Dimos

Spore-forming Bacteria: Food

Industry

pore-forming Toxin Producers:

Spore-forming Food Spoilag
Clostridum botulinum Alicyclobacillus
Clostridum perfringens Geobacillus
Sporolactobacillus
Bacillus cereus

Clostridium botulinum

Bacterial Spore Production

Spore Structure

Dipicolinic acid
(DPA)
Setlow et. al. 2006

DPA-less spores: lower

resistance to wet heat,
dry heat, hydrogen
peroxide, and

Targeted Gene Knock out by

Homologous Recombination
CSLD 3

CSLD Genomic 3

CSLD 3

Selection

Target Site

Selection

CSLD 3

CSLD Genomic 3

CSLD 3

Small acid-soluble proteins