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15

30

3000rpm 10m

RNA

Cell dissolution 100ul

1M DTT 5m u

ul

homogenize 5s in ice cool down 10s 15000rpm 30m TCA

Cell dissolution 100ul 12000rpm 10m 4

1M DTT

ul

RNA 3 30 RNA
¾

TRIZOL 1ml 3000rpm 10m

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