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c 

 
2 p Two Slide or Wedge Method
2 p Two Cover Slip Method or Erlich Method
× p Cover Glassand Slide Method or 
c 

        
2 p 
 

a p Wright Stain
b p Giemsa Stain
c p eishman Stain
d p  
 

a p enner- Giemsa Stain


b p May- Grunwald- Griemsa Stain
c  
2 p Staining Dish Method
2 p Staining Jar or DIP Method
× p Œ tomatic Method
c     
2 p our ʹield Meander Method
2 p Two ield Method
× p Exaggerated Battered Method
4 p Strip Differential Method

   


      
  
  Broken into segment Small pinkish stains 60-70%
Or 55-70%

  Closely knitted; usually ight Blue 25-×5% up to 40%
round
   Immature and found
rarely; Usually mistaken as
Monocytes
  Mature, typical
  
   Younger form of 2-6%
neutrophils with a C, S or
horse-shoe shape
appearance
! 
  Has 2 lobes a large coarse 2-4%
reddish or orange
granules
c  "     Spongy Sprawling with ight gray 2-6%
      brain- like coagulation
 
  Indistinct and appear Purplish black or bluish 0-2%
burned under large Granules

Criteria of a Good Blood Smear


2 p The thick area makes a gradual transition to the thin area (feather-like edge)
2 p The blood in the thin area does not extend to the end of the side
× p Must have smooth and even surface
4 p eukocytes must be clumping
RECOMMENDED ORDER O DRAW OR PHEBOTOMY

Evacuated Tube System No of Inversion Additive


ight Blue ×-4 Sodium Citrate
Yellow 8 ACD (Acid Citrate Dextrose)
Gold 5 SST (Serum Separating Tube)
Red(Glass) 0 None
Red (plastic) 5 Clot activator
ight Green 8 Plasma Separating Tube
Green 8 Sodium Heparin
Purple 8 K2EDTA; K×EDTA
Gray 8 Potassium Oxalate