‘Anal Bioanal Chem. sages se rcrerio}I216-010-3975-2 ORIGINAL PAPER. Rapid detection of Aspergillus flavus in rice using biofunctionalized carbon nanotube field effect trans Raquel A. Villamiza Marote - F. Xavier Rius R.A. Vilanioar“A, Manto “FX, Bits (@) Deparmentaf Analytical and Oya: Chenisty, Univesiat Roviai Visi, Meal, Danning, sn, 49007 Taragons Spain ‘onal Savlenmtauvest Boole Supéiare de Chinie Cyaniqe et Mingras (ESCOM), EA 4297 TIMR, 1 ale durian Jen Mie Bude, '50009 Conpitene, Fane Deparment of Misobiiogy, Univesity of Panplna, Kam 1, Via Buearamangs, N de §, Calor, Abstract In the present study, we have used carbon, nanombe field effect transistors (FET) that have been functionalized with protein G and IgG to detect 4spergillus flavus in contaminated milled rice. The adsorbed protein G on the carbon nanotubes walls enables the IpG anti- Aspergillus antibodies to be well oriented and therefore to splay full antigen binding capacity for fungal antigens. A solution of Tween 20 and gelatine was used as an effeetive blocking agent to prevent the non-specific binding of the antibodies and other moulds and alsa to protect the transducer against the interferences present in the rice samples. Our FET devices were able to detect at least 10 e/g of 4. flavus in only 30 min, To evaluate the selectivity of our biosensors, Fusarnen oxysporum and Penicillin chrysogenum were tested as potential competing moulds for 4. flavus. We have proved that our devices are highly selective tools for detecting mycotoxigenic moulds at low concentrations real samples, one Hmmm? posts * Pp Fest Foseoutsion noes dtc five wth CMTE Ptein Ceti e satbosis9Wspopielyevintted wie PBST SEidc he Nf emcee onthe anaes fas dlssd by meant fae gen moody Blew Enea