Annual differences in daily variations in hepatic drug metabolizing enzyme activity and plasma

hormone levels in the rat.

Daily variations in nonclinical toxicology hepatic drug metabolism and in plasma corticosterone and
insulin levels were examined at monthly intervals over the duration of 1 year. Annual differences were
observed in the basal levels of all parameters. Consistent daily variations were observed only in plasma
corticosterone levels, whereas daily variations in each of the other parameters occurred inconsistently
over the year with significant variations being found in ethylmorphine metabolism and insulin levels
only five times, and in aniline metabolism, six times. These data show that daily variations in the
preclinical toxicology parameters examined do not occur in a regular manner.

Comprehensive Health Effects Testing Program for Denver's Potable Water Reuse Demonstration
Project.

The Comprehensive Health Effects Testing Program for the Denver Water Department's Potable Water
Reuse Demonstration Project is designed to evaluate the relative health effects of highly treated
reclaimed water derived from secondary wastewater compared to Denver's present high-quality
drinking water. The 1 million gallon per day (1 mgd) demonstration plant provides water to be
evaluated in the studies treating unchlorinated secondary treated wastewater with the following
additional processes: high pH lime clarification, recarbonation, filtration, ultraviolet irradiation,
activated carbon adsorption, reverse osmosis, air stripping, ozonation, and chloramination. An
additional sample is obtained from the identical treatment process substituting ultrafiltration for reverse
osmosis. The toxicology tests to evaluate the possible long-term health effects are chronic toxicity and
oncogenicity studies in Fischer 344 rats and B6C3F1 mice and reproductive/teratology in Sprague-
Dawley rats. The results of these evaluations will be correlated with microbiological, chemical, and
physical test results to establish the relative quality of reclaimed water compared to all established
health standards as well as Denver's pristine drinking water.

Find additional information on Toxicology Consultants at GWTox.com.