stem mioLocy "TEACHER'S MANUAL 2072008 Experiment 1 (Practical No. 2(a)) Title: Deteminnto ofthe eso ptetial ftp clap Purpose: |. To prem the scrote solutions of various molt fom 810 td sak soon 2 Toplan and candi the experiment ffikty 3. Tomensure and eon he eg of tae cel 4, Total tee ety 5, Toanays and inert xperienta resus based owl or theny Apparatus and materials Diao ate Boing es laced in beshers Peas Fier paper dh ih cover Marker pen 150. ang od waoden stk 05 cn in tr, with 2Scat measuring coder or sro = 13m 1m pipe Procedure 1 abled boing tbs, repre 20! of srs olin of wolrtes Pol dv’, (02 ml din 03 ol dm ml de nd 0 mol fen he sok solo sng he lution Wecige The viumes ofthe slog ad fhe dated war wel soul be ‘eco in te bilo, Moly ‘aim | 02m | 03M | oam | osm Volume af [Mure solu) Votune of ised water (om) Mie emivaSPM BIOLOGY ‘TEACHER'S MANUAL 20072008 2 Prepare 1S spe of otto tes 4 cm 0 6 cm long and with eruescton of ‘bem 04 em, Record he average length ofthe pot tips. ‘ion of potato stripe. Take 3 pla sip record the lng ofeach fhe sip and Place the psn te boing he. Repeat hs sep using the Serose soluons of malas [2a dors 03 mol 0 mol dn and 0S mol dr 4 Aer 30 minut, remote te sis with the wooden tick povided, Wipe dem ge ‘i their pper ore he inl length ofeach of he ota sp, 5. Resor the ial eel of the sucrose solution in each of he oiling tabs and ote down ‘ry change th psi onditon of th oa sp, 6 Caleta te average change in length Table 7 Othe emph paper, () Pht a snd gaph ofthe osmotic potential gst the molarity of the sure solution dtermine the esate potential of he oto cll sp. vwiaiy [ows [ovo] ons [oar] 03s [a0 [038] aon [ose [aan [ose (Plt a raph ofthe average shane in he length asi the molarity ofthe eros soln,STPM BIOLOGY ‘TEACHER'S MANUAL 200772008 Discussions (@) From the two graphs, determine (the osmotic concentration ofthe potato tissues in mal den of sucrose solution, the osmotic potential in atm,STPM BIOLOGY ‘TEACHER'S MANUAL 200772008, ‘Teacher's Guide ‘Scheme: Experiment 1 (Practical No. 2(a)) |. Manipulative skill (A) Marks 1. Planning ofthe set up of the experimental apparatus 2. Dilution has been done corectly 3. Preparation of potato strips 4. Correct usage oF apparatus Sang pline at work (work independently ~ without asking t00 much ‘questions or distracting other candidates) 2. Product (B) 1. Tabu jon of tell 1 Boiling tube 01m [02M [03M [0am | 05M ‘Average final length of 3 strips ram) ‘Average initial length of 3 strips (mm) ‘Average change in length of tips Final level of solution (mm) Initial level of solution (min) ‘Change in level of solution (mm) Physical condition of strips atthe end of experimentSTPM BIOLOGY ‘TEACHER'S MANUAL 20072008, 2. Recording of data: ~ Length of strips (il up atleast 3 columns) 1 = Level of solution (rise/dop) ~ (fil upat least 3 columns) 1 = Physical condition of strips (ill up atleast 3 columns) 1 Increase in length o | 0 04 06 Decrease Molar ml n3 in lengthcaning Results: Melarity tim 02m | esm | dm | bm [Volume sf tom cere | 2.0 [0-0 | 6.0 | fo [ooo | seludicn Com) | | i | Volume of dishile d eo 4.0 2.0) 10.0 water Cond) Molanty Jov0s oto [15 |e.20 0.28] 0.40 [0.55] 0.40 |0.¥8]¢.50 [bb Osmske Peerkal ae) t-8 nolo Lea tpbte (ab) wi ab Wal te boiling tube O1M 0.2m 05m | tM 05M Dverage tinal length of ebipe lon) $1.2 S05 | WAb) N40 | UES Buage inal loylh ef 2ceplon) $4.0 Fe S10 0 | 308 _Diveenge chon ge in Taeglh of spate) tea S00 | 08 | 10 | ache Final level st slates (may [50-0 | pv | ye | 51.0 | 0.0 | Jrihial Lod of caladvn (ad [810 [51.0 | suo [sro] 53.0 | ChargestniVevel of sloben (un) (cts [Ain | 0.0 | 90 | Lose) Dhyseal eu dbon ab sys ab [Tog Trg Bled Ene Flow | tha end of exparion ontQuishens (n) From thr tae graphs. determine (Abe csmebe ceatendenbvs of Be pulaly Lasnes in wel dnt of sucrose solution 0:24 fu) the egmehe potendal in ale. bb ate (6) Evplan why yeu have chosen the above wolariby, There tent change in dhe length of the potter stupas the Sucrese elution v9 ssefomic fo the call There is np not tovimert of Water fom the cel sop ty the sucrose sluben ond vce versa ial sabidvnc sl whvert