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The Effect of Ginseng On Lumbriculus: Variegatus
The Effect of Ginseng On Lumbriculus: Variegatus
on Lumbriculus
variegatus
HCOP PE 2008
Ginseng Facts
• History
• Antioxidant
• Ginsenosides
• Dose/response relationship
Pulsation Rate:
Hypothesis
• Ginseng will lower the pulsation rate
in Lumbriculus variegatus.
Experimental Design
Pulsation Rate
12 worms 12 worms
12 worms
Treatment
p=<0.0001
Time p=0.0003
Treatment*Tim
e p=0.0056
Hypothesis-
Locomotion
• Ginseng will increase locomotion in
Lumbriculus variegatus.
Experimental Design
p/m=probes
Locomotion per minute
12 worms
12 worms
12 worms
6 6
worms worms 6 6
6 p/m 12 p/m 6 6
worms worms
worms worms
6 p/m 12 p/m
6 p/m 12 p/m
Material and Methods
Locomotion
• N= 12 worms in 1 mL spring water (control
group)
• N= 12 worms in 1 mL 12µg% ginseng (treatment
group 1)
• N=12 worms in 1 mL 24µg% ginseng (treatment
group 2)
• Constructed a race track for the worms
• Exposed 12 worms in each solution for ten
minutes
• Place one worm on the track and probed every 5
seconds for one minute and measured distance.
• Placed one worm on the track and probed every
10 seconds for one minute and measured
distance.
Statistical Analysis
• Calculated mean and standard deviation
Time
p=0.8934
5 seconds
Chemotaxis Hypothesis
Control
Treatment 1 Treatment 2
(spring water)
(ginseng 6µg%) (ginseng 12µg%)
20 20 20
worms worms worms
Materials and Methods
• N=20 worms, individually, added one drop
of spring water at tail and observed
reaction
• N=20 worms, individually, added one drop
of 6 µg% ginseng and observed reaction
• N=20 worms, individually, added one drop
of 12 µg% ginseng and observed reaction
• Recorded data
Chemotactic Test Results
Conclusion
• In conclusion, when Lumbriculus
variegatus are subjected to a low
concentration (12 µg%) for 10 minutes,
the pulsation rate increases. In support of
our hypothesis, when exposed to 24 µg%
ginseng, pulsation rate decreased.
• Contrary to our hypothesis, ginseng did
not affect the locomotion of California
blackworms.
• Contrary to our hypothesis, ginseng
produced a positive chemotactic response
.
Discussion
• Although ginseng is prized for its
antioxidant benefits, it appears to
have stimulatory effects on the
vasculature of worms. More research
is needed to determine if this is true
in humans.
Works Cited
• Drewes, Charles PhD. Those wonderful worms.
Lumbriculus variegatus Lab. Department of
Zoology and Genetics University of Iowa.
Accessed 2008 June 18.
• http://kmsayre.blogspot.com
• http://nlloughry.blogspot.com
• http://johnnaprice.blogspot.com