HOPANICAL Gazer oe J. Re Yoon and AJ Gare 198 Feit ‘pene, Pe Pi 2: NCS te Ph of abe ams, Aon Sa aE A an ti rh a cna te Henin io of ie open gies at een stage rel ae med cnt ene saci. 99) Sau he lees produced Wy apples, PageiC ES a oman oe tui EET RE BE open cova MORPHOLOGICAT, AND HISTOCHEMICAL STUDIES OF PRIMARY ROOT MERISTEM OF RAUWOLTIA YOMITORIA, Aer, J. np eas a PAAR Jn the Reso sus tp he brn chr by te of here hr an ering tha Then hae es a In previous conmnicatons (Qty and Paria, 1968, 1968; Maa, 1968), histchemical analyses uf nayineaetvites fn the meristematic cll of the shoot spect of Revco coerent, R.mans, Re Pen and R somone rented th Spel ‘rphasison the nstion af sleds These studs have toa esteale to the prinuy root mersten ‘of Rawulia tomitors, incxponting observations ‘on the iiiation of rot hair nthe bypadrmis, ‘Daring the hist 0 years there es bee Hite ew inforaation segardng inition ap development of root his frm the bypodermis of waar pants (Ceswr, 190; Pissaros, 1936; Havwsab aa owe, 1942). During the same pera conierable saleable information hes nscale ot eat hes of epidermal engin (Comsace, 1949, 1962 Baye, 19S Caos 11) A detain or ae ade, therfore, to Muy maternal Sakic Hot bhi originate rms hyposerlei Tens for Rstocheriel Investigations oxiginte from the recent reports. rgurig.istocheiel inulyet of the psimaty root mere of sever festucoid grasses made by Avis (1938, 1061), ‘vers and Grit (108%, 8c), and_ Avie td Kise (1040), They demoratrated that epilental teot hair tricia) hl mre insted ‘Chasm aetves thon ld the harlese sister eal ‘Thuy concluded that the naphogenetc manifests tion of trichobless in the grassoot epidermis was Coneated ith the dferentiaton sd diferent netlsiie of enaymes, Im the present study iso Chemie ste were cared att with ton of tondorie in an atempt to assy the piysolgical "this ef emo enayme sams ering ination of ‘dhoblst in Ue hypodennis and to rate these Fests to those obtsined in epee tichnblasts ob festucei urs.196 MIA & PATHAK—RAUWOLFIA ROOT TIP 208, ‘Material and methods Splints of Racwifo rotor were recived from Costs Rica hough the courtesy of Dt. Hos trann, Chu Lid, Suitertend. They wee gow a ‘rdnacy greece sil in. porcelsn pots inthe {gevahoue of Teas Rear Fatman, Kerner, ‘Tas "Two applications of tlie 10 ce S phosphors potasium) were ade during ne frorng period. esthy root fie wee collected ffs the vigorously rowing plants and ual rah for stashed fest Avis of hve espe Ssuemsy perovdte ad Satan (8 hy stereo yen the hires oe {set ond Aten snd Grn 195), "To lie ponies activity 2mm sot tips sere inated for 20-80 ace fn Solution equa fare af 03% benitoe and 02 codon sive Pristde—then for 30-00 se in Soon Lk~cqual Burt of Soation 1 and 0.005%; bydrpen pero Aer inebation, the rot tips ere Haw ques ‘nailed water and mounted on gla ses for ‘ctermined by the appearence of being ive Cobration. For conc, roo ts were inated Sn anne of Slutns Tan itt wiih 005% KCN war alld to inactivate the eye. Forde teeing est of ne the techniques Aves {aoe es follower Justa 1962. The tot Ue were incubated at 39°C for min na medium onsintng of 1100 mg ahi Cac 263 mg Sigsowriio, sd 174g mse adeylc aed di tole in 10 mi 01 4 Tes alse baer stp TR The peut of enayme acy wae ae Ve ine ly the cobaltue method. For contol tot tip wee fneubated i the above medium for Sate Containing 001 2F NaF to inevte the ence AtTcat thie tits weve erie gut foreach enzyme bys and the rls lsined ere consent in i Observations the inition and development of soot sree made py fry the et Preparations nd intronic tis of Cy cst, bot para sens ove ln sade Po the late, rot iw were ed in Crt ly dehy raed ins tertizy buylaleool series and tedden Tinmit. Sel acetone ut 86 by wer stoied in sultan sad bento Obcerratins Iseratiit oF roo maik—Te anatomy of the roottip meristem of Rawlia omiors as aloady teen deserbed trots median logics. (N39 195), The rote ichoblosts frente fn the bypoderni which e composed of neti! sous of lensing short and ong cell "Tho abort cle mature aa trlehoblaets and moquentiy Form toot als the Jong coll ema hares theeughout de. telopment (Bp. 4) The tchoblists ae dncasible Ss plysiologealydiserte cells in regan appro Ietely 10D hack fom the soap merstem, we Uhr morphological ifereataian at root irs eeu ata distance of $00 yTrom the rot sex (ig) In Lngisctions of young rots (fg), the Uechobasts are reepminhle nee of thet she utr walls and hart! shape, transection, Towever, they are camspicunus primarily bruise ol thal sting reaeions 3). Figure a stace ew of the rot meristem ia which he “square {cobs in he hyper ca be seen thrash ‘he epidermis The epidermal cel sheathing he ret ite are mice orl stongate i 5 "With the item of roots fst, the trchoblste fom outward protruding papilla; at this stage they resemble inaure promi The papas elage snd then emerge as distinc 00k hes by rupturing the epitermal els immediately feral tthe ig 8). Ruplates of the ep ‘Kemal all walls, reins fem the emergence af ‘oot hairs, ate offen canspiewus Ia the oder pot tothe zo wher the cot bare have dsatgnated but the eidermis ha not ee Been sloushed of igs 210) ‘Resoias oF mstoenewtea, 1p318~-The hit chemical analvse lr peosve showed Ut mare Intesedeuayie arty occured in the roo-alt frchoblats than the long hile hypoderal fells the her showed atively Ite or an setirity {fap 5,8) The devceping soot hr alo howe ‘iocous perosiane activity (Oe 2 6, 8) lr the tcivty eecainallycgpcntrted fn intracltae nganeles (og. 8}, Granule particles showing ete ymecctvity staining cceueed ip the epidermal Cellsof soe oat (ig, 9). Reot-cap cells aso showed oxide activity (2), Conta ot tip showed ro peroidane atv “Teste for Sancl showed it to be active in the techs an: Not al he tichoblot athe sme rot showed an oqually intense activity, omever Ug. TH, 12). "No Snel etilty was confined to slesly ssl intracellular portiles(Egs 11,12) 26 hasbeen opoced fe festa grasses (AVF, 1951) ‘The cone rot tips didnot show si clarion resting rn enayme sli ‘The pinay sot meristem of Reel snore hos bien shown to contin Rypoerms charute Tend by eral ows of altemating sr thst vn ess collin general ugrecrient wilh the frie reports f Leaver (1000 Thora a ques: ‘So, however, concerning Bow the ret hairs exterge from the hypaderis. Leaver (£008) steed thatILA & PATHAK —RAUWOL -Eachriaamassnio te gpvrmi a ihr slonghid if or fs cols wepurated Irorexe ther above the niegng trchobiss 10 make 3 passage Tur ihe femeeging mt Baim Havas ad Lose (1B) ound tha tncepiermiswassloughed of in Valeacia ‘ange tats while scree (1990) sear that In Rios disor, Fraswensio attra, aod Pio ‘toda condone she epral lly ere puso fil the enlarging hyper oot has lng Ahir wy to the exterior. tn Kenora te fond seat sty, the eminence of et ts et the epider tes place Dy Trchablsts, ton fstance was there tlthors pushing asi of he epider elle by the Kyocera sot hes os slooehing of prot Tore the emergence of tot ais Te the tearing of the epider) cel walls sound Uy the developing not ala as yet eer sn, but the rape bow certain degre of regu larly. A mere physia presure developed fa ep lero aly euarping Foot hairs would be expet- fel to yd egal aptures While soe py our tr 207 ial premure apparent, cannot be the only ctr Teang to breakage of the epileanal walls because the wendeaey toward syramety a the Tpiues “Tress oceur in abndonce fn the hyper ris of Konig tots, stb! eects for pvoidise nol Sane indicated that ore vigoros ney atfiiy cane the shor ego che cists dn ithe ong huldss ste cls Sine ty nf these enase sere was cae nthe upd Gaon, 195%; Aves, 196). The poset inves Signin sun howe thet peo nt Sel wre ive in the macuring vont hats ax wel Tes ob Srvations om Ror substanite the bypass ih epidermal or ypederm sth eer Keen an he seit alist of exyines et paral stig i vdope ( AES, 158, $1) Nvres od ou, J In R somite the rots misc Gactating sooteaphareagie est HH Pees ety anpearel fist vchablass 1 bey the rota BOTANICAL GAZEFTE tip merister. ‘Tas the marten i apne fore the sone of active donation by mart sone {100 41 neh no peoutase atvy was bere (Gg. 2) "These ndings in Kameotfo do aot quite gree with those in festucold gars pared By ‘Sere and Gene (10380) tn the Iter plans peo Save acy acer In sone 800-1,000 fr ‘he rot apes, that inthe sone i which the ate of cal dongaton deceased om 2 maximum oo. In Rosia, erence in peroidone tivity ‘etteen the mero ell elongation snd materaton fanrot be etablshed became peruse ally fist apears na nme where cell congation i ust starting 200 back of te rot tip) apd extends oush the sone af cell ongaton isto the sone oF saturation, Thus a poysialogialy active zone for petoidase activity, such as sce in fstoeold gases, Fett spre inte rot ese of tomar, Inthe festa rosie the roottip meister and the tone of perosidase activity is sqparatad by nazov zoe that Licks peroxidase activity jst a8 in 2 comoria, This upgets Pot a base smarty fo pysolgial pattem does exist betneen the ser ens of thee two plogeneticaly unrelated plans ‘Was Purr (1959, 181) reported the oecnrrence ‘of vigorous pevoxidae etvty inthe endoderas of roots of several perce of plants In R. semen, howeve, very litle pecoidase activity occured the oot endoderm (8g, 3 Acknowledgment We expess our sncereappieiaion to our col longue Eowaxo L, Wiutivos, Bikop Colle, ‘Dali, Tens, who sce ue in various ways during the corse of this invention Breese eet gegen onetnceay inte dar sean dell Se Pas omate See ceca Tovar ote Jha Doge WSN van, Crintene jan se 10, Hicker cages FN 1, Jn apn neers Bol Sie Conan CT SB, The eek bi aa Ronn 180 Pat ant. J Wey Sm, Feu 003 asa ho ea hehe 9.9 Preman Mao Wi cope rin hm neta Origey and etn of ssi in owes. an fay sieTeNT phy a wean, 4, Mena Kilt Statin Aa niet th st of Samet spe 1 Ceca gam MH Slay et Ain, RoR. Gat te Van ans OS. ot. A