BT1002 PLANT BIOTECHNOLOGY 3 0 0 100

AIM
To develop the skills of the students in the area of Plant Biotechnology.

OBJECTIVES
At the end of the course the student would have learnt about the applications of Genetic Engineering in Plant and
how to develop Transgenic plants. This will facilitate the student to take up project work in this area.

UNIT I ORGANIZATION OF GENETIC MATERIAL 9

Genetic material of plant cells – nucleosome structure and its biological significance; junk and repeat sequences;
outline of transcription and translation.
UNIT II CHLOROPLAST & MITOCHONDRIA 9
Structure, function and genetic material; rubisco synthesis and assembly, coordination, regulation and transport of
proteins. Mitochondria: Genome, cytoplasmic male sterility and import of proteins.

UNIT III NITROGEN FIXATION 9

Nitrogenase activity, nod genes, nif genes, bacteroids.

UNIT IV AGROBACTERIUM & VIRAL VECTORS 9

Pathogenesis, crown gall disease, genes involved in the pathogenesis, Ti plasmid – t-DNA, importance in genetic
engineering. Viral Vectors: Gemini virus, cauliflower mosaic virus, viral vectors and its benefits.

UNIT V APPLICATION OF PLANT BIOTECHNOLOGY 9

Outline of plant tissue culture, transgenic plants, herbicide and pest resistant plants, molecular pharming, theraputic
products.

TOTAL : 45
TEXT BOOKS
1. Gamburg OL, Philips GC, Plant Tissue & Organ Culture fundamental Methods, Narosa Publications. 1995.
2. Singh BD. Text Book of Biotechnology, Kalyani Publishers. 1998

REFERENCES
1. Heldt HW. Plant Biochemistry & Molecular Biology, Oxford University Press. 1997.
2. Ignacimuthu .S, Applied Plant Biotechnology, Tata McGraw-Hill. 1996.