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  • Thesis 2

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    Supriya Potluri
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    This document outlines the aim and objectives of a study to design novel human MEK1 protein inhibitors for potential melanoma treatment through ligand-based virtual screening. The objectives include target identification and validation of MEK1, phylogenetic analysis, identifying the MEK1 binding site, and using software to identify and optimize lead compounds. The methods section describes retrieving the MEK1 protein sequence from the UniProt database and performing proteomic analysis.

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    This document outlines the aim and objectives of a study to design novel human MEK1 protein inhibitors for potential melanoma treatment through ligand-based virtual screening. The objectives include target identification and validation of MEK1, phylogenetic analysis, identifying the MEK1 binding site, and using software to identify and optimize lead compounds. The methods section describes retrieving the MEK1 protein sequence from the UniProt database and performing proteomic analysis.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
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    16 views1 page

    Thesis 2

    Uploaded by

    Supriya Potluri
    This document outlines the aim and objectives of a study to design novel human MEK1 protein inhibitors for potential melanoma treatment through ligand-based virtual screening. The objectives include target identification and validation of MEK1, phylogenetic analysis, identifying the MEK1 binding site, and using software to identify and optimize lead compounds. The methods section describes retrieving the MEK1 protein sequence from the UniProt database and performing proteomic analysis.

    Copyright:

    Attribution Non-Commercial (BY-NC)
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    of 1

    AIM AND

    OBJECTIVES
    Aim:
    Ligand based virtual screening to design novel human MEK1 protein inhibitors for
    potential development of drugs against melanoma.

    Objectives:
     Target identification.
     Target validation.
     Phylogenetic analysis.
     Identification of the binding site.
     Lead identification and optimization using Ligand-Info and Schrodinger software suite
    2009(Maestro 9.0).

    MATERIALS AND METHODS


    Target Identification
    3.1 Retrieval of protein sequence
    UniProt is the universal protein resource, a central repository of protein data created by
    combining Swiss-Prot, TrEMBL and PIR. This makes it the world's most comprehensive
    resource on protein information. The UniProt Consortium comprises the European
    Bioinformatics Institute (EBI), the Swiss Institute of Bioinformatics (SIB), and the Protein
    Information Resource (PIR). Until recently, EBI and SIB together produced Swiss-
    Prot and TrEMBL, while PIR produced the Protein Sequence Database (PIR-PSD).
    The human, epidermal growth factor receptor (EGFR) protein sequence was
    accessed and retrieved from http://www.uniprot.org.html.
    3.2 Proteomic analysis of EGFR protein

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