AS 3000 Operating Manual

HOME AI 3000 / AS 3000
3000 Series A u tomatic Sampling System
Operating Manual
AI 3000/AS 3000 Operating Manual
Edition September 2004
Part Number 317 093 92
© 2004 Thermo Electron S.p.A. All rights reserved..
Printed in ITALY.
Published by Thermo Electron Italia-Milan, Technical Documentation, Strada Rivoltana 20090 Rodano - Milan
Tel: 02 95059 373 Fax: 02 95059388
Printing History: First edition, released January 2002

Second Edition, released May 2003
Third Edition, released September 2003
Fourth Edition, released April 2004
Fifth Edition, released September 2004
Disclaimer
Technical Information contained in this publication is for reference purposes only and is subject to change without notice. Every effort has
been made to supply complete and accurate information; however, Thermo Electron assumes no responsibility and will not be liable for any
errors, omissions, damage, or loss that might result from any use of this manual or the information contained therein (even if this information
is properly followed and problems still arise).
This publication is not part of the Agreement of Sale between Thermo Electron and the purchaser of a AI/AS 3000. In the event of any
conflict between the provisions of this document and those contained in Thermo Electron’s Terms and Conditions, the provisions of the
Terms and Conditions shall govern.
Reference to System Configurations and Specifications supercede all previous information and are subject to change without notice.
Trademarks
TRACE™ and FOCUS™ are trademarks of Thermo Electron S.p.A.. Other product names or marks may be registered names or trademarks
of other companies.
Declaration
Manufacturer: Thermo Electron S.p.A.
Thermo Electron S.p.A. is the manufacturer of the instrument described in this
manual and, as such, is responsible for the instrument safety, reliability and
performance only if:
• installation
• re-calibration
• changes and repairs
have been carried out by authorized personnel and if:

• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its
operation is only entrusted to qualified trained personnel
Thermo Electron S.p.A. is not liable for any damages derived from the
non-compliance with the aforementioned recommendations.
Thermo Electron S.p.A.

Strada Rivoltana
20090 RODANO (MI)
ITALY
Operating Manual iii

iv Operating Manual
Contents
About This Manual.................................................................................................................................ix
Overview ................................................................................................................................... ix
Conventions Used in This Manual............................................................................................. x
Instrument Markings and Symbols ..........................................................................................xii
Chapter 1
Introduction........................................................................................................................................ 15
Classification of the Instrument ............................................................................................... 16
Environmental Conditions ............................................................................. 16
Safety Information ................................................................................................................... 16
The AI 3000 / AS 3000 ............................................................................................................ 17
Sampling Unit ............................................................................................................. 18
Base................................................................................................................ 18
Turret.............................................................................................................. 18
Injection Assembly ........................................................................................ 18
Washing and Waste Tray................................................................................ 18
Centering Plate............................................................................................... 18
Sample Tray ................................................................................................... 18
Sampler Support............................................................................................. 19
User Interface.............................................................................................................. 19
Configurations of the 3000 Series Automatic Sampling System............................................. 20
AI 3000 Autoinjector Configuration........................................................................... 20
AS 3000 Autosampler Configuration ......................................................................... 21
Installation................................................................................................................... 21
Right configuration ........................................................................................ 21
Left Configuration ......................................................................................... 22
Technical Specifications .......................................................................................................... 23
Chapter 2
Sampling Unit..................................................................................................................................... 25
Base .......................................................................................................................................... 26
Turret........................................................................................................................................ 28
Injection Assembly ..................................................................................................... 29
Vial Capture Device.................................................................................................... 30
Syringe ........................................................................................................................ 30
Movements of the Turret............................................................................................. 32
Washing and Waste Tray ......................................................................................................... 33
Rear of the Sampling Unit........................................................................................................ 34
Operating Manual v
Contents
Sample Trays ........................................................................................................................... 35

8-Position Sample Tray .............................................................................................. 35
105-Position Sample Tray Assembly ......................................................................... 35
Vials............................................................................................................... 36
Centering Plate......................................................................................................................... 37
Status LED............................................................................................................................... 38
Chapter 3
Installation ..........................................................................................................................................39
Preliminary Information .......................................................................................................... 40
Who Performs the Installation.................................................................................... 40
Electrical Requirements.............................................................................................. 40
How to Lift and Carry the Sampling Unit .................................................................. 40
Support of the Autoinjector/Sampler.......................................................................... 41
References for the Installation.................................................................................... 44
Installation of the AI 3000 on the GC ..................................................................................... 50
Installation of the AS 3000 on the GC..................................................................................... 54
Chapter 4
AI 3000/AS 3000 Control ....................................................................................................................61
Control from PC ...................................................................................................................... 62
User Interface ............................................................................................................. 62
Sampling Section........................................................................................... 63
Injection Section............................................................................................ 64
Washes Section.............................................................................................. 64
Info Line ........................................................................................................ 66
Status Line ..................................................................................................... 66
Description of the Menus ........................................................................................... 66
File Menu....................................................................................................... 66
Edit Menu ...................................................................................................... 67
Command Menu ............................................................................................ 68
Messages..................................................................................................................... 69
Control through the GC ........................................................................................................... 70
References for Programming...................................................................................... 70
Programming through the TRACE GC Ultra ............................................................. 70
How to Configure the Autoinjector/Sampler ................................................ 70
Menu of the AI 3000/AS 3000 ...................................................................... 71
Extended Control Menu ................................................................................ 72
vi Operating Manual
Contents
When No Vial Abort Menu............................................................................ 73

Programming through the FOCUS GC....................................................................... 74
How to Configure the Autoinjector/Sampler ................................................ 74
AI 3000/AS 3000 Menu................................................................................. 75
Sequence of Samples with the AS 3000 ........................................................ 77
Guidelines for Programming with Different Injectors ............................................................. 79
S/SL Inlet (Trace/Focus)............................................................................................. 79
Nano-Volumes Injections in S/SL Inlets .................................................................... 80
S/SL Inlet with a Merlin Microseal™......................................................................... 82
PTV Inlet..................................................................................................................... 83
PTV Inlet with a Merlin Microseal™ ......................................................................... 84
PKD and PPKD Inlets................................................................................................. 84
Chapter 5
Maintenance ....................................................................................................................................... 85
Current Maintenance................................................................................................................ 86
Appendix A
“Space Saving” Installation ............................................................................................................. 93
Appendix B
Installation on the Left Side of the FOCUS GC MS Version ......................................................... 95
Appendix C
Customer Communication ............................................................................................................... 99
How To Contact Us.................................................................................................................. 99
Europe ......................................................................................................................... 99
Africa, Asia and Oceania .......................................................................................... 101
North, Central and South America............................................................................ 102
Reader Survey ........................................................................................................... 103
Glossary ..............................................................................................................................................105
Index ....................................................................................................................................................111
Operating Manual vii

Contents
viii Operating Manual

About This Manual
Overview
This Operating Manual contains descriptions of the features and components of
the AI 3000/AS 3000 Systems. Inside, you will find all of the information
necessary for routine operation of your autosampler. This includes operating
procedures, sample injection techniques, and diagrams and descriptions of the
major components.
This manual is organized as follows:
Chapter 1, Introduction, provides a basic overview of the features and options of

the AI 3000/AS 3000 System. It also describes the available instrument
configurations.
Chapter 2, Sampling Unit, provides a description of the components of the

sampling unit of the AI 3000/AS 3000 System.
Chapter 3, Installation, contains the instructions for the installation of the

AI 3000/AS 3000 on the TRACE GC Ultra and FOCUS GC gas chromatographs,
the syringe and the electrical connections with the different units of the gas
chromatographic system.
Chapter 4, AI 3000/AS 3000 Control, provides the information to control the

AI 3000/AS 3000 from the computer or the GC. The chapter contains also the
working procedures with different injectors.
Chapter 5, Maintenance, provides guidelines for the maintenance of the

AI 3000/AS 3000.
Appendix A, “Space Saving” Installation, contains the installation sequence of

the AS 3000 to reduce its working area.
Appendix B, Installation on the Left Side of the FOCUS GC MS Version, contains

indication to install the AI 3000/AS 3000 on the left side of the FOCUS GC MS
Version.
Appendix C, Customer Communication, contains contact information for Thermo

Electron offices worldwide. Use the Reader Survey in this section to give us
feedback on this manual and help us improve the quality of our documentation.
ix
About This Manual Conventions Used in This Manual
The Glossary contains definitions of terms used in this manual. This also includes
abbreviations, acronyms, metric prefixes, and symbols.
The Index contains an alphabetical list of key terms and topics in this guide,
including cross references and the corresponding page numbers.
Conventions Used in This Manual

The following symbols and typographical conventions are used throughout this
manual.
Bold Bold text indicates names of windows, menus, dialog boxes,

buttons, and fields.
Italic Italic indicates cross references, first references to important
terms defined in the glossary, and special emphasis.
Monospace Monospace, or Courier, indicates filenames and filepaths, or
to indicate text the user should enter with the keyboard.
Monospace Monospace Bold indicates messages or prompts displayed on
Bold the computer screen or on a digital display.
» This symbol illustrates menu paths to select, such as
File»Open….
KEY NAME Bold, uppercase sans serif font indicates the name of a key on
a keyboard or keypad, such as ENTER.
This symbol alerts you to an action or procedure that, if
CAUTION performed improperly, could damage the instrument.
This symbol alerts you to important information related to the

NOTE text in the previous paragraph.
x
Conventions Used in This Manual About This Manual
This symbol alerts you to an action or procedure that, if

WARNING! performed improperly, could result in damage to the
instrument or possible physical harm to the user. This symbol
may be followed by icons indicating special precautions that
should be taken to avoid injury.
This symbol indicates electric shock hazard.
This symbol indicates danger from hazardous chemicals.
This symbol indicates danger from high temperature surfaces

or substances.
This symbol indicates a fire hazard.
This symbol indicates an explosion hazard.
This symbol indicates a toxic hazard.
This symbol indicates the presence of flammable materials.
This symbol indicates the presence of radioactive material.
This symbol indicates an operation or procedure that must

NOT be performed by the user. A Thermo Electron
authorized Customer Support Engineer must perform this
procedure.
xi
About This Manual Instrument Markings and Symbols
This symbol indicates all metal objects, such as watches,

jewels, etc., must be taken off.
This symbol indicates an eye hazard. Eye protection must be

worn.
This symbol indicates the user must wear a protective screen

when performing the procedure.
This symbol indicates the user must wear protective shoes

This symbol indicates the user must wear protective clothing

This symbol indicates the user must wear gloves when

performing the procedure.
Instrument Markings and Symbols

The following table explains the symbols used on Thermo Electron instruments.
Only a few of them are used on the AI 3000/AS 3000.
Symbol Description
Direct Current
Alternating Current
Both direct and alternating current
Three-phase alternating current

3
xii
Instrument Markings and Symbols About This Manual
Symbol Description
Earth (ground) terminal
Protective conductor terminal
Frame or chassis terminal
Equipotentiality
On (Supply)
Off (Supply)
Equipment protected throughout by DOUBLE

INSULATION or REINFORCED INSULATION
(Equivalent to Class II of IEC 536)
Instruction manual symbol affixed to product. Indicates
that the user must refer to the manual for specific
Warning or Caution information to avoid personal
injury or damage to the product.
Caution, risk of electric shock
Caution, hot surface
Caution (refer to accompanying documents)
xiii
Symbol Description
In-position of a bistable push control
Out-position of a bistable push control

Introduction
1
This chapter provides a basic overview of the features and options of the AI 3000/
AS 3000. It also describes the available instrument configurations.
Chapter at a Glance…
Classification of the Instrument............................................................................ 16
Safety Information................................................................................................ 16
The AI 3000 / AS 3000 ........................................................................................ 17
Configurations of the 3000 Series Automatic Sampling System ......................... 20
Technical Specifications ....................................................................................... 23
Operating Manual 15
Chapter 1
Introduction Classification of the Instrument
Classification of the Instrument

Environmental Conditions
• Internal use
• Up to 2000 meters altitude
• Temperature 18 to 30 °C
• Maximum relative humidity between 30% and 85%
• Voltage variations must not exceed the nominal voltage by ± 10%
• Transient overloads in compliance with installation categories II
• Pollution degree according to IEC 664 (3.7.3) 2
• Protection degree IP00
Safety Information
WARNING! When, for technical reasons, it is necessary to work on instrument parts which
may involve an hazard (moving parts, components under voltage, etc.) the
authorized Technical Service must be contacted. This type of situations can be
identified because access to these parts is possible only by using a tool. The
removable protective covers bear a warning symbol suggesting to refer to the
documentation accompanying the instrument.
Should an operator perform a maintenance operation, he/she must have received
proper training to carry out that specific action. Before using dangerous
substances (toxic, harmful, etc.), please read the hazard indications and
information reported in the Safety Sheet supplied by the manufacturer referring to
the relevant CAS (Chemical Abstract Service) number.
The AI 3000/AS3000 requires the use of several chemical products, which are
present in vials and syringes, having different hazard characteristics. Before
using these substances or replacing the syringe, please read the hazard
indications and information reported in the Safety Sheet supplied by the
manufacturer referring to the relevant CAS (Chemical Abstract Service) number.
16 Operating Manual
Chapter 1
The AI 3000 / AS 3000 Introduction
The AI 3000 / AS 3000

The 3000 Series Automatic Sampling System, available as AI 3000 or AS 3000,
consists of the following parts, as shown in Figure 1-1:
1. Safety door 2. Turret

3. Injection unit 4. Centering plate
5. Base 6. Washing and waste tray
7. 8-position sample tray 8. 105-position sample tray
Figure 1-1. AI 3000 and AS 3000 Diagram
Operating Manual 17
Chapter 1
Introduction The AI 3000 / AS 3000
Sampling Unit
The sampling unit consists of the following major components:
Base
This is the structure bearing the sampling unit components.
Turret
It consists of a vertical moving structure that accommodates the injection unit and
accomplishes the necessary movements to carry out the three main operating
steps: sample drawing, injection, syringe washing. The turret is provided with a
safety door allowing access to the syringe.
Injection Assembly
Housed inside the rotating turret, it consists of a moving support (sliding plate) on
which the syringe is installed.
Washing and Waste Tray

It allows to accomodate up to four 4 mL vials of solvents to wash the syringe, and
a 50 mL plastic container to collect the washing solvents after their use.
Centering Plate
It consists of a plate provided with a circular guide to center the sampling unit on
the injector nut.
Sample Tray
• It contains the seats for the sample vials. Each position is numbered to make
the sample identification easy. The following options are available:
• Autoinjector AI 3000
Sample tray consisting of an 8-position fixed support.
• Autosampler AS 3000
Sample holding assembly consisting of a 105-position turnable mobile tray
mounted on a dedicated support plate.
18 Operating Manual
Chapter 1
The AI 3000 / AS 3000 Introduction
Sampler Support
It constitutes the supporting base allowing to install the autoinjector/sampler on
the gas chromatograph.
User Interface
The functions of the AI 3000 / AS 3000 can be controlled through:
• a data processing system for PC with dedicated software.
• the GC keypad.
For further information, please refer to:

• Paragraph Configurations of the 3000 Series Automatic Sampling System on
page 20
• Chapter Sampling Unit on page 25.
• Chapter AI 3000/AS 3000 Control on page 61.
Operating Manual 19
Chapter 1
Introduction Configurations of the 3000 Series Automatic Sampling System
Configurations of the 3000 Series Automatic

Sampling System
The AI 3000 / AS 3000 can be installed on the TRACE GC Ultra or FOCUS GC
gas chromatographs for sample introduction into the following injectors:
— TRACE GC Ultra: S/SL, PTV, PKD, PPKD
— FOCUS GC: S/SL
The autoinjector/sampler is available in the following options:

• AI 3000 Autoinjector Configuration
• AS 3000 Autosampler Configuration
AI 3000 Autoinjector Configuration

In this configuration the 8-position sample tray is used.
Figure 1-2. AI 3000 Autoinjector
As a function of the configuration of installation, right or left, the support will be

introduced into the relevant side housing on the base of the sampling unit. On the
opposite side housing, the centering plate will be positioned.
20 Operating Manual
Chapter 1
Configurations of the 3000 Series Automatic Sampling System Introduction
AS 3000 Autosampler Configuration

In this configuration the 105-position sample tray is used, mounted on the hub
provided on the dedicated support plate.
Figure 1-3. AS 3000 Autosampler
The support plates of the tray and the sampler are electrically connected to each
other by means of the proper connecting cable.
As a function of the configuration of installation, right or left, the support will be
introduced into the relevant housing on the base of the sampling unit.
Installation
The AI 3000 / AS 3000 can be installed on the right or left side of the GC
according to the following indications:
Right configuration
The right configuration, shown in Figure 1-4, is the default configuration both for
the TRACE GC Ultra and the FOCUS GC.
The autoinjector/sampler installation uses the same type of support on both GCs.
Operating Manual 21
Chapter 1
Introduction Configurations of the 3000 Series Automatic Sampling System
Figura 1-4. Right Configuration
NOTE The right configuration is the only one possible on the FOCUS GC.
Left Configuration
This configuration, shown in Figure 1-5, is only possible with the TRACE GC.
The autoinjector/sampler installation requires a dedicated metal support on the
base of the autoinjector/sampler support.
Figure 1-5. Left Configuration
WARNING! If an existing AI 3000 Autoinjector will be upgraded to AS 3000 Autosampler, it is

very important to switch off the AI 3000 before to install the 105 sample tray and
plug-in its electrical cable. This recommendation will prevent a possible driver
failure on AI3000’s pcb.
22 Operating Manual
Chapter 1
Technical Specifications Introduction
Technical Specifications
Table 1-1. Technical Specifications
Sample tray capacity - 8 vials in the AI 3000 configuration
- 105 vials in the AS 3000 configuration
Vials capacity 0.3 and 2 mL (standard) with both 8- and 105-position trays
Flushing solvents 4 vials of 4 mL
Waste container 50 mL
capacity
Types of syringes 10 µL syringe (standard)
10 µL syringe for Merlin Microseal Valve (optional)
5 µL syringe (optional)
0.5 µL syringe for Nano-volumes injections (optional)
Volumes programming 0 to 5 µL (10 µL syringe) with increments of 0.1 µL
(µl)
0 to 2.5 µL (5 µL syringe) with increments of 0.1 µL
0 to 0.5 µL (0.5 µL syringe) with increments of 0.01 µL
Programmability Remote
External interface Serial line RS 232
Power supply 24 Vdc through a portable external power supply
Electrical characteristics of the supply
- input 90-264 Vac; 47/63 Hz
- output 24 Vdc; 70 W
Dimensions Sampling unit:
(height x width x
AI Configuration approx. 437 x 230 x 250 mm
length)
AS Configuration approx. 437 x 400 x 250 mm
Weight About 6 kg
Operating Manual 23
Chapter 1
Introduction Technical Specifications
24 Operating Manual
Sampling Unit
2
This chapter provides a description of the components of the sampling unit of the
AI 3000/AS 3000.
Base ...................................................................................................................... 26
Turret .................................................................................................................... 28
Washing and Waste Tray ......................................................................................33
Rear of the Sampling Unit.................................................................................... 34
Sample Trays ........................................................................................................35
Centering Plate ..................................................................................................... 37
Status LED............................................................................................................ 38
Operating Manual 25
Chapter 2
Sampling Unit Base
Base
It is the supporting structure of the instrument on which the sampling unit
components and accessories are positioned (sample tray, centering plate). It
consists of the following sections, as shown in Figure 2-1
Front section Right side section
Left side section Rear section
Figure 2-1. Sections of the Base

• Front Section
It includes the housings for solvents vials and waste container.
On the lower front part there is a LED indicating the instrument status. For
details, refer to Status LED on page 38.
• Right - Left Side Sections
NOTE The right-left side sections can be distinguished from the front section (washing and waste
tray).
It can accomodate the following accessories:
• 8-position sample tray
• 105-position sample tray
26 Operating Manual
Chapter 2
Base Sampling Unit
• centering plate for the injector.
The position of the above described accessories depends on the configuration

of the autoinjector/sampler installation.
See Configurations of the 3000 Series Automatic Sampling System on page

20.
• Rear Section
It includes the connections of the instrument to the mains, the gas
chromatograph, the data system and the sample tray (only for AS 3000).
Operating Manual 27
Chapter 2
Sampling Unit Turret
Turret
The turret consists of a moving structure pivoted on a hub. The rotating
movements are accomplished by a motor located on the base. An optical sensor
determines the turret travel ends.
The front side is provided with a safety door allowing access to the injection
assembly. The door opening immediately cuts off power supply to the
autoinjector/sampler.
.
Figure 2-2. The Turret
28 Operating Manual
Chapter 2
Turret Sampling Unit
Injection Assembly
It consists of a sliding plate which supports and guides the vertical movements of
the injection device constituted by the syringe plunger control, the vial capture
device and the syringe.
2
1
4
5
5
1. Safety door 2. Sliding plate

3. Injection device 4. Syringe plunger control
5. Syringe 6. Vial capture device
Figure 2-3. Injection Assembly
Operating Manual 29
Chapter 2
The vertical movements of the injection device are controlled by motors housed in
the turret. The travel ends of the movable parts is defined by a series of sensors.
Inside the turret two electronic boards are also present, one for the motors control
and one for the instrument functions control, respectively.
Vial Capture Device

The lower part of the injection device consists of the vial capture device, shown in
Figure 2-4, which has the following functions:
• to guide the syringe needle
• to acknowledge the vial presence
• to acknowledge the injector presence
.
Figure 2-4. Vial Capture Device
Syringe
The standard syringe has 10 µL capacity with 50 mm needle length.
NOTE 5 and 0.5 µL syringes are optionally available.
A thorough washing of the syringe can be done thanks to the possibility of using
up to four solvents. See Washing and Waste Tray.
To install or replace the syringe, please refer to the Chapter Installation and to the
Chapter Maintenance.
30 Operating Manual
Chapter 2
Turret Sampling Unit
Figure 2-5 shows the syringe housing.
1. Syringe Housing 2. Syringe
Figure 2-5. Syringe Housing
Operating Manual 31
Chapter 2
Movements of the Turret

The sequence of the turret movements allows the autoinjector/sampler to perform
the following functions:
• Sample drawing — the turret positions itself on the selected vial, housed in
the sample tray, to allow the syringe to draw the sample it contains.
• Sample injection — the turret positions itself on the injector to perform the
sample injection.
• Syringe washing — the turret positions itself on the solvents vials for the
syringe washing. The solvent is automatically withdrawn and discharged into
the waste container.
32 Operating Manual
Chapter 2
Washing and Waste Tray Sampling Unit
Washing and Waste Tray

The washing and waste tray, shown in Figure 2-6, can contain up to four 4 mL
vials and a 50 mL plastic container.
The vials contain the solvent (solvents) necessary for the syringe washing, which
can take place both before and after the injection. The washing solvent is then
collected in the waste container.
1. Waste container 2. Vials for solvents A and B

3. Vials for solvents C and D
Figure 2-6. Washing and Waste Tray
The four vials containing solvents for the syringe washing are introduced into the
appropriate seats A, B and C, D, on the left and on the right of the waste container
respectively, marked WASTE.
Operating Manual 33
Chapter 2
Sampling Unit Rear of the Sampling Unit
Rear of the Sampling Unit

The rear side of the sampling unit includes the following components, as shown in
Figure 2-7:
• Jack marked 24 Vdc for the instrument power supply through the external
portable feeder.
• 15-pin female connector (double density) marked TRAY for the
communication between the sampling unit and the motor actuating the hub
positioned on the supporting plate of the 105-position sample tray.
• 9-pin female connector marked RS232 for the communication between the
autoinjector/sampler and the GC (connector marked AUTOSAMPLER)
through a proper cable.
• 6-pin female connector marked GC for connecting the autoinjector/sampler to
the GC (connector marked SAMPLER SIGNAL) through the proper cable.
.
Figure 2-7. Rear of the Sampling Unit
34 Operating Manual
Chapter 2
Sample Trays Sampling Unit
Sample Trays
The AI 3000 / AS 3000 can accept two types of sample trays which are
accommodated in one of the two side housings on the autoinjector/sampler base.
The type of sample tray accessory installed on the instrument (8 or 105 positions)
is automatically acknowledged by the autoinjector/sampler when switched on,
and it determines the instrument configuration.
• The Autoinjector AI 3000 uses the 8-position sample tray.
• The Autosampler AS 3000 uses the 105-position sample tray.
8-Position Sample Tray

The 8-position tray, shown in Figure 2-8, consists of a fixed arc-shaped support
and can contain up to 8 vials numbered 1 to 8.
The slot present on the tray determines the position 1 and allows to inspect the
level of the liquid contained in the vial.
.
View from the top Front view
Figure 2-8. 8-Postiion Sample Tray
105-Position Sample Tray Assembly

The assembly, shown in Figure 2-9, consists of a dedicated supporting plate
provided with a hub and a 105-position removable sample tray.
The rotation of the tray is accomplished by a motor, located under the supporting
plate, which actuates the hub thanks to suitable commands coming from the
Operating Manual 35
Chapter 2
Sampling Unit Sample Trays
sampling unit via an interfacing cable.

An optical sensor defines the rotation travel end and the vial position.
Supporting plate of the 105-position tray 105-position sample tray
Figure 2-9. Supporting Plate and 105-Position Sample Tray
The tray can contain up to 105 vials numbered 01 to 105 arranged on three
circular rows.
• Vials 01 to 40 are placed into the external row.
• Vials 41 to 80 are placed into the middle row.
• Vials 81 to 105 are placed into the internal row.
Vials
Vials of 0.3 and 2 mL capacity can be used.
The 0.3 mL vial is exactly the same as the 2 mL vial, provided inside with a 0.3
mL calibrated insert.
CAUTION Vials must be accurately closed using appropriate septa and ring nuts.
Septa must be those recommended by Thermo Electron. The use of septa with
different characteristics might damage or bend the syringe needle.
During the vials preparation, it is recommended to comply with applicable safety
regulations, specially as far as the conditions of the workplace ventilation are
concerned.
36 Operating Manual
Chapter 2
Centering Plate Sampling Unit
Centering Plate
The centering plate, shown in Figure 2-10, consists of a plate provided with a
circular guide to center the sampling unit on the injector nut.
Figure 2-10. Centering Plate
CAUTION Two types of centering plates are available with the TRACE GC Ultra according to
the injector mounted.
- A standard centering plate to center the sampling unit on the SSL, PKD and PPKD
injector nut.
- A centering plate to center the sampling unit on the PTV injector nut. It is
recognized by a circular slot present on the circular guide.
Operating Manual 37
Chapter 2
Sampling Unit Status LED
Status LED
The status LED, located on the front side of the sampling unit, as shown in
Figure 2-11, provides the operator with indications on the instrument operating
conditions showing a continuous or intermittent light.
.
1. Status LED
Figure 2-11. Status LED
Condition LED
St-by Lit with continuous green light
Position zeroing, Intermittent green light; 0.5 seconds ON, 0.5 seconds OFF
sample preparation,
washing, injection
Run (waiting GC Intermittent green-orange lights; 0.5 seconds green, 0.5
Ready) seconds orange.
Firmware upload Intermittent orange light; 0.5 seconds ON, 0.5 seconds OFF
Error Lit with continuous orange light
Buzzer
Two beep monotone; 0.5 seconds ON, 0.5 seconds OFF, 0.5 seconds ON when
the start signal is received.
Five consecutive beep, 1 second with interval of 0.5 seconds OFF in case of error
condition.
38 Operating Manual
Installation
3
This chapter contains the instructions for the installation of the AI 3000/AS 3000
on the TRACE GC Ultra and FOCUS GC gas chromatographs, the syringe and
the electrical connections with the different units of the gas chromatographic
system.
Preliminary Information ....................................................................................... 40
Installation of the AI 3000 on the GC .................................................................. 50
Installation of the AS 3000 on the GC ................................................................. 54
Operating Sequences
How to Install the Autoinjector/Sampler Support on the TRACE GC Ultra ....... 45
How to Install Autoinjector/Sampler Support on the FOCUS GC.......................49
How to Install the AI 3000 on the GC.................................................................. 50
How to Install the AS 3000 on the GC................................................................. 54
How to Install the Syringe.................................................................................... 58
Operating Manual 39
Chapter 3
Installation Preliminary Information
Preliminary Information
This chapter contains the preliminary information for the installation and
connection of the autoinjector/sampler to the TRACE GC Ultra and FOCUS GC,
the electrical requirements and the autoinjector/sampler support.
Who Performs the Installation

NOTE The AI 3000/AS 3000 is installed by authorized Thermo Electron technical engineers, who
will check its correct operation. For more details, please contact Thermo Electron local
representatives. Should the instrument not be installed by Thermo Electron personnel,
please strictly adhere to the following instructions.
Electrical Requirements
The instrument must be electrically supplied as indicated in paragraph Technical
Specifications in Chapter 1.
WARNING! The power line and the connections between the instruments must maintain good
electrical grounding. Poor grounding represents a danger for the operator and
may seriously affect the instrument performance.
Do not connect the AI 3000 /AS 3000 to lines feeding devices of a heavy duty
nature, such as motors, UV lamps, refrigerators and other devices that can
generate disturbances.
Pay attention not to leave any cable connecting the sampling unit and the
chromatographic system or the power cord close to the GC hot air vents.
How to Lift and Carry the Sampling Unit

The autoinjector/sampler must be lifted and carried by hand, as shown in
Figure 3-1.
40 Operating Manual
Chapter 3
Preliminary Information Installation
Figure 3-1. How to lift and carry the Sampling Unit
Support of the Autoinjector/Sampler

The AI 3000/AS 3000 is installed on the GC using the appropriate support
provided (see Figures 3-2 and 3-4).
The AI 3000/AS 3000 is installed in the following way:

• TRACE GC Ultra; on the right or left side
• FOCUS GC; only on the right side
NOTE The FOCUS GC MS Version may accept the AI 3000/AS 3000 also on the left side. Refer
to Installation on the Left Side of the FOCUS GC MS Version.
Operating Manual 41
Chapter 3
NOTE To reduce the FOCUS GC working area, the AS 3000 may be installed as described in
Appendix “Space Saving” Installation.
Support for TRACE GC Ultra

The support for the installation of the AI 3000/AS 3000 on the TRACE GC,
shown in Figure 3-2, consists of a semi-circular plate resting on three spacers non
adjustable in height. The top surface of the plate is provided with two slots for
corresponding fixing screws. In the middle the guide pivot allows the
accommodation and centering of the sampling unit.
.
2
1 4
1. Semi-circular plate 2. Guide Pivot

3. Spacers 4. Slots
5. Fixing screws
Figure 3-2. Support for the installation of the AI 3000/AS 3000 on the TRACE GC
When installing the AI 3000/AS 3000 on the TRACE GC Ultra right side, the
autoinjector/sampler support is directly mounted on the GC top panel as shown in
Figure 3-2, previous installation of the spacer inside the GC.
42 Operating Manual
Chapter 3
When installing the AI 3000/AS 3000 on the TRACE GC Ultra left side, before to
mount the autoinjector/sampler support, the support bracket must be placed and
fixed on the GC top cover as shown in Figure 3-3.
.
1 1
2 2
3 3
3 3
3
2 3
2 2
2
4
4
1. Support Bracket 2. Holes on the TRACE GC Ultra Top Cover

3. Support Bracket Fixing Screws 4. Autoinjector/Sampler Support
Figure 3-3. Bracket for the installation of the AI 3000/AS 3000 Support
Operating Manual 43
Chapter 3
Support for FOCUS GC

The support for the installation of the AI 3000/AS 3000 on the FOCUS GC,
shown in Figure 3-4, consists of two semi-circular plates. The top surface of the
upper plate is provided with two slots for corresponding fixing screws. In the
middle the guide pivot allows the accommodation and centering of the sampling
unit.
.
3 1
1. Upper Semi-circular Plate 2. Lower Semi-circular Plate

3. Guide Pivot 4. Slots
5. Fixing Screws
Figure 3-4. Support for the installation of the AI 3000/AS 3000 on the FOCUS GC
References for the Installation

For the installation of the AI 3000/AS 3000, please refer to:
• How to Install the Autoinjector/Sampler Support on the TRACE GC Ultra on
page 45
44 Operating Manual
Chapter 3
• How to Install Autoinjector/Sampler Support on the FOCUS GC on page 49

• Installation of the AI 3000 on the GC on page 50.
• Installation of the AS 3000 on the GC on page 54.
OPERATING SEQUENCE
How to Install the Autoinjector/Sampler Support on the TRACE
GC Ultra
To correctly install the support on the TRACE GC Ultra, please refer to the
instructions reported in the following operating sequence:
Before installing the autoinjector/sampler support, the spacer should be installed

inside the GC. Refer to Figure 3-5.
1. Remove the cover and the collar of the injector and detector compartment.
2. Open the column oven door. Loosen the two knobs located in the upper part.
3. Push the cover backwards and remove it.
4. Screw the spacer on the threaded pin.
5. Put the GC cover back in the original position.
6. Tighten the two knobs. Close the column oven door.
7. Mount the spacer fixing Allen screw.
8. Reinstall the cover and the collar of the injector and detector compartment.
Operating Manual 45
Chapter 3
Figure 3-5. Installation of the Spacer inside the GC
Assembling of the Autoinjector/Sampler Support on the GC right side

The following is the description of how to mount the autoinjector/sampler support
in the default configuration, i.e. on the right side of the TRACE GC Ultra.
1. Remove from the GC upper cover the two plastic caps covering the
corresponding fixing holes.
2. Insert into each slot present on the support the provided fixing screw.
46 Operating Manual
Chapter 3
3. Insert each screw into the relevant spacer paying attention to keep its largest
surface turned towards the support base.
4. Holding the spacers in position with your hands, rest the support on the GC
upper cover, with their flat side towards the inside.
5. Guide the two fixing screws located on the external spacers into the
6. Loosely tighten the screws.
Assembling of the Autoinjector/Sampler Support on the GC Left side

in the on the left side of the TRACE GC Ultra.
1. Remove from the GC upper cover the four plastic caps covering the
2. Mount the autoinjector/sampler support bracket on the TRACE GC Ultra and

fix by using fixing screws provided
3. Insert into each slot present on the autoinjector/sampler support the provided
fixing screw.
4. Insert each screw into the relevant spacer paying attention to keep its largest
surface turned towards the support base.
Operating Manual 47
Chapter 3
5. Holding the spacers in position with your hands, rest the support on the
bracket, with their flat side towards the inside.
6. Guide the two fixing screws located on the external spacers into the
corresponding fixing holes located on the bracket.
48 Operating Manual
Chapter 3
OPERATING SEQUENCE
How to Install Autoinjector/Sampler Support on the FOCUS GC
To correctly install the autoinjector/sampler support on the FOCUS GC, please
refer to the instructions reported in the following operating sequence:
Assembling of the Autoinjector/Sampler Support on the GC right side

in the default configuration, i.e. on the right side of the FOCUS GC.
.
3. Guide the two fixing screws into the corresponding fixing holes located on the
GC upper panel.
Operating Manual 49
Chapter 3
Installation Installation of the AI 3000 on the GC
Installation of the AI 3000 on the GC

To correctly install the AI 3000 on the TRACE GC Ultra and FOCUS GC gas
chromatographs, please refer to the instructions reported in the following
operating sequence:
OPERATING SEQUENCE
How to Install the AI 3000 on the GC
The procedure includes the following steps:
• Installation of the sampling unit
• Installation of the syringe
• Electrical connections
• Startup
Installation of the Sampling Unit (Right Configuration)
1. Lift the sampling unit, as shown in Figure 3-1, and insert it into the guide pin
located on the autoinjector/sampler support.
.
1. Insert the centering plate into its seat located in the left side section of the
sampling unit base paying attention that the guide hole, present on the arm of
the centering plate, correctly fits the injector nut.
50 Operating Manual
Chapter 3
Installation of the AI 3000 on the GC Installation
Centering Plate
FOCUS GC TRACE GC Ultra
2. Check the correct alignment of the autoinjector/sampler support, then fix it by

tightening the proper fixing screws.
Operating Manual 51
Chapter 3
Installation Installation of the AI 3000 on the GC
3. Insert the 8-position sample tray into the appropriate housing located in the
right side section of the sampling unit base.
Installation of the Sampling Unit (Left Configuration)

This operation is possible only with the TRACE GC Ultra.
The steps required are those described in the sequence Installation of the
Sampling Unit (Right Configuration), but positioning the centering plate on the
right and the 8-position sample tray on the left.
Installation of the Syringe

To install the syringe follow the instructions reported in the How to Install the
Syringe operating sequence on page 58
Electrical Connections
The instrument must be powered as indicated in Technical Specifications in
Chapter 1. Before performing electrical connections, ensure that the requirements
reported in paragraph Electrical Requirements on page 40 have been fulfilled.
Proceed as follows:
1. Using the cable provided, connect the 6-pin female connector marked GC
located on the sampling unit back side and the connector marked SAMPLER
SIGNAL located on the GC rear panel.
52 Operating Manual
Chapter 3
Installation of the AI 3000 on the GC Installation
2. Using the cable provided, connect the 9-pin female connector marked RS232
located on the sampling unit back side to 9-pin connector marked
AUTOSAMPLER located on the GC rear panel.
Instrument Startup
1. Plug in the Vdc power cable of the external portable power supply into the
jack marked 24 Vdc located on the sampling unit back side.
2. Connect the power cord of the external power supply to the mains outlet.
The AI 3000/AS 3000 will automatically run the self-testing routine during which
the following automatic checks and settings are carried out:
— alignment between AI 3000/AS 3000 and injector
— acknowledgement of the installed sample tray
— calculation of the syringe zero
NOTE The self-test rountine is automatically carried out every time that the safety door of the
turret is closed.
Operating Manual 53
Chapter 3
Installation Installation of the AS 3000 on the GC
Installation of the AS 3000 on the GC

To correctly install the AS 3000 on the TRACE GC Ultra and FOCUS GC gas
chromatographs, please refer to the instructions reported in the following
operating sequence:
OPERATING SEQUENCE
How to Install the AS 3000 on the GC
The operation includes the following steps:
• Installation of the sampling unit
• Installation of the syringe
• Electrical connections
• Startup
Installation of the Sampling Unit (Right Configuration)
1. Lift the sampling unit, as shown in Figure 3-1, and insert it into the guide pin
located on the autoinjector/sampler support.
.
2. Insert the centering plate into its seat located in the left side section of the
sampling unit base paying attention that the guide hole, present on the arm of
54 Operating Manual
Chapter 3
Installation of the AS 3000 on the GC Installation
the centering plate, correctly fits the injector nut. Refer to Centering Plate on
page 37.
.
Centering Plate
FOCUS GC TRACE GC
3. Check the correct alignment of the autoinjector/sampler support, then fix it by

tightening the proper fixing screws.
Operating Manual 55
Chapter 3
4. Insert the dedicated support plate into its appropriate housing located in the
right side section of the sampling unit base. Place the 105-position sample
tray on the hub located on the support. The system will automatically
recognize the sample tray at the instrument power on.
Installation of the Sampling Unit (Left Configuration)

The operation is possibly only with the TRACE GC Ultra.
The steps required are those described in the sequence Installation of the
Sampling Unit (Right Configuration) but positioning the centering plate on the
right and the 105-position sample tray on the left.
Installation of the Syringe

To install the syringe follow the instructions reported in the How to Install the
Syringe operating sequence on page 58.
56 Operating Manual
Chapter 3
Electrical Connections
The instrument must be powered as indicated in Technical Specifications in
Chapter 1. Before performing electrical connections, ensure that the requirements
reported in paragraph Electrical Requirements on page 40 have been fulfilled.
Proceed as follows:
1. Connect the 15-pin female connector of the cable coming from the support
plate of the 105-position sample tray to the connector marked TRAY located
on the sampling unit back side.
2. Using the cable provided, connect the 6-pin female connector marked GC
located on the sampling unit back side and the connector marked SAMPLER
SIGNAL located on the GC rear panel.
3. Using the cable provided, connect the 9-pin female connector marked RS232
located on the sampling unit back side to 9-pin connector marked
AUTOSAMPLER located on the GC rear panel.
Instrument Startup
1. Plug in the Vdc power cable of the external portable power supply into the
jack marked 24 Vdc located on the sampling unit back side.
2. Connect the power cord of the external power supply to the mains outlet.
The AI 3000/AS 3000 will automatically run the self-testing routine during which
the following automatic checks and settings are carried out:
— alignment between AI 3000/AS 3000 and injector
— check of the turret travel
— acknowledgement of the installed sample tray
— calculation of the syringe zero
NOTE The self-test rountine is automatically carried out every time that the safety door of the
turret is closed.
Operating Manual 57
Chapter 3
OPERATING SEQUENCE
How to Install the Syringe
The installation of the syringe is a simple operation. However, it must be
performed with caution to avoid damages to the syringe needle and ensure an
optimal performance of the injection device.
NOTE 5 and 0.5 µL optional syringes are optionally available.
1. Open the safety door of the turret.
Referring to Figure 3-6.
2. Insert the syringe needle into the vial capture device.
3. Accomodate the syringe body into its seat paying attention to insert
simultaneously the flange and head of the syringe plunger into their relevant
guides.
Referring to Figure 3-7
4. Lock the syringe by turning clockwise the lock knob by approx. 180°.
5. Close the safety door of the rotating turret.
58 Operating Manual
Chapter 3
5
4
3
7
1
1. Syringe Needle 2. Syringe Body

3. Flange 4. Plunger
5. Plunger Head 6. Vial Capture Device
7. Syringe Body Seat 8. Flange Guide
9. Plunger Head Guide
Figure 3-6. Installation of the Syringe (1)
Operating Manual 59
Chapter 3
1. Lock Knob
Figure 3-7. Installation of the Syringe (2)
60 Operating Manual
AI 3000/AS 3000 Control
4
This chapter provides the information to control the AI 3000/AS 3000 from the
computer or the GC. The chapter contains also the working procedures with
different injectors.
Control from PC ................................................................................................... 62
Control through the GC ........................................................................................ 70
Guidelines for Programming with Different Injectors .........................................79
Operating Sequences
How to Pack a S/SL Liner .................................................................................... 81
Operating Manual 61
Chapter 4
AI 3000/AS 3000 Control Control from PC
Control from PC
The AI/AS 3000 is controlled through a data system (e.g. Chrom-Card) installed
in the PC and interfaced with the instrument via serial line RS 232.
This paragraph describes how to program the instrument operating parameters.
IMPORTANT Before creating an autosampler method you must configure the correct syringe
capacity in the AI/AS 3000 Configuration page of the Data System in use (refer to
your Data System Operating Manual – Configuration for details).
User Interface
The user interface, shown in the example of Figure 4-1, is the same for both
AI 3000 and AS 3000 configurations. In this page the AI 3000/AS 3000
parameters can be set, whereas the sequence of samples to be analyzed and
acquired is managed by the data system as a function of the type of sample tray
installed on the autoinjector/sampler.
NOTE The page is displayed after the software in use has been selected in the configuration page
referring to the relevant operating manuals.
Figure 4-1. User Interface for AI 3000/AS 3000
62 Operating Manual
Chapter 4
Control from PC AI 3000/AS 3000 Control
The page of Figure 4-1 is subdivided into four sections.
Sampling Section
In this sections the autoinjector/sampler parameters can be set.
Sample volume (µl)

This parameter specifies the sample volume to be injected as a function of the
syringe installed.
With a 10 µl syringe, choose a value between 0 and 5 µl in steps of 0.1 µl.
With a 5 µl syringe, choose a value between 0 and 2.5 µl in steps of 0.1 µl.
With a 0.5 µl syringe, choose a value between 0 and 0.5 µl in steps of 0.01 µl.
Plunger strokes
It allows di specify the number of plunger strokes to eliminate air bubbles forming
during sample drawing.
Set a value 0 to 15.
Viscous sample
This parameter defines the sample drawing speed as a function of the sample
viscosity.
Choose one of the following options:

• No (default value) if the sample has low viscosity.
• Yes if the sample has high viscosity.
Sampling depth in vial:

This parameter specifies the penetration depth of the syringe needle into the vial.
Set the parameter choosing one of the following options:
• Bottom (default value)
The needle goes down to the vial bottom.
• Center
The needle goes down to half vial.
Select Center if the sample vial may contain solid residues on the bottom.
Operating Manual 63
Chapter 4
Injection Section
In this section the injection parameters are selected.
Injection depth:
This parameter defines the penetration depth of the syringe needle into the
injector.
Set the parameter choosing one of the following options:
• Standard (default value)
The needle goes into the injector to the maximum possible depth.
• Minimum
The needle enters the injector and stops immediately beyond the septum.
Use Minimum only if injecting with cold needle (Cold Needle technique)
NOTE When Minimum is selected, Pre and Post-inj dwell time (s) boxes are not enabled.
Pre-inj dwell time (s)

This parameter is active when the option Standard has been selected in Injection
depth:.
This parameter specifies how long the syringe needle must remain inside the
injector before injection. This allows the needle to be heated before the sample
injection. (Hot Needle Injection).
Set in this box the delay time between 0 and 63 seconds.
Post-inj dwell time (s)

This parameter is active when the option Standard has been selected in Injection
depth:. This parameter specifies how long the syringe needle must remain inside
the injector after injection. This allows the complete evaporation of any residues
present inside the needle.
Set in this box the delay time between 0 and 63 seconds.
Washes Section
In this section the parameters of pre- and post-washing with solvent, and washing
with sample are specified.
64 Operating Manual
Chapter 4
Pre Injection Box

In this box the parameters of pre-washing with solvent are specified.
Solvent
To set the vial containing the pre-washing solvent. The AI 3000/AS 3000 can use
up to 4 different solvents; it is possible to define which solvent, or combination of
two solvents, must be used before injection selecting one of the following options:
• A, B, C, D, A+B, C+D.
If A+B or C+D are selected, the autoinjector/sampler will use both solvents in
sequence. Different combinations from A+B or C+D are not possible.
Cycles
This parameter allows to set how many syringe pre-washing cycles with solvent
have to be run before injection.
Set a number between 0 and 15.
Sample Box
In this box the parameters of the syringe washing with sample are set.
Rinses:
It allows to set the number of syringe pre-washing cycles with sample to be run.
Select a number between 0 and 15.
Post Injection Box

In this box the parameters of the syringe post-washing with solvent are set.
Solvent
It allows to specify the vial containing the post-washing solvent. The AI 3000/AS
3000 can use up to 4 different solvents; it is possible to define which solvent, or
combination of two solvents, must be used after injection selecting one of the
following options.
• A, B, C, D, A+B, C+D.
If A+B or C+D are selected, the autoinjector/sampler will use both solvents in
sequence. Different combinations from A+B or C+D are not possible.
Operating Manual 65
Chapter 4
Cycles
This parameter allows to set how many syringe post-washing cycles with solvent
have to be run after injection.
Set a number between 0 and 15.
Info Line
It displays the range of the selected parameter.
Status Line
It displays the instrument status messages and error messages that may occur
during operation. See Messages on page 69
Description of the Menus

File Menu
The File menu contains the following items:
New
This function creates a new method for the autoinjector/sampler starting from the
data currently in memory or from default parameters previously stored with the
function Save As Default.
Open
This function uploads a pre-existing analytical method for the autoinjector/
sampler from disk to the computer memory.
Open Recent...
It allows to retrieve the last 5 saved analytical methods.
NOTE To activate a loaded or modified method, use the command Send Method to
Sampler.
Save (Save as...)

This function allows to save an analytical method on the hard disk. With Save
as... the method can be saved with a new name.
66 Operating Manual
Chapter 4
Load from Default

This function allows to upload a default analytical method.
Save As Default
This function allows to save the current analytical method as a default method for
the autoinjector/sampler (....\tmp..\AI/AS3000 Default method.asm). The
parameters of this method can be retrieved when selecting the function New.
Get Method from Sampler

It allows to transfer the autoinjector/sampler current method to the GC.
Send Method to Sampler

It transfers the method from the computer to the autoinjector/sampler and makes
the method active.
Print
This function allows to print the method.
Close
This function allows to close the method. If modifications have been done, the
system will ask where to save them in the method.
Edit Menu
The Edit menu gives access to the following functions:
View Audit Trail

It displays the log file of the method. Each change to the method parameters is
recorded on the log file with the operator’s name as well as the date and time of
the modification. Using the function Copy it is possible to copy part or all the log
file contents into Windows and then transfer information to other programs if
required.
NOTE Audit trail method log is available only if a protection policy is active in the data system.
Operating Manual 67
Chapter 4
Command Menu
The Command menu gives access to the following functions:
Start Clean Cycle

It allows to start a washing cycle.
Inject Single Sample

It allows to start the injection of a single sample.
Force Autoinjector to Stand-by

It interrupts any current operation and forces the autoinjector/sampler to stand-by.
Menu Help
It allows to have access to the on-line guide.
68 Operating Manual
Chapter 4
Messages
This paragraph lists the messages that the computer may display according to the
autoinjector/sampler status.
Status Messages
• Opening Connection...
• No connection (Check the serial line connections)
• The AI 3000/AS 3000 is in stand-by
• AI 3000/AS 3000 is checking positions
• AI 3000/AS 3000 ready to inject sample in vial n but waiting GC Ready.
• AI 3000/AS 3000 is preparing the sample in vial n.
• AI 3000/AS 3000 has injected the sample in vial n.
• The AI 3000/AS 3000 front door is open (all operations are paused)
Error Messages
• AI 3000/AS 3000 error, solvent not found
• AI 3000/AS 3000 position error, can’t find the initial zero positions
Operating Manual 69
Chapter 4
AI 3000/AS 3000 Control Control through the GC
Control through the GC

The AI 3000/AS 3000 functions are controlled and programmed through the gas
chromatograph keypad.
NOTE It is not possible to control the 0.5 µL syringe through the GC keypad but only through the
Data System.
References for Programming

For the AI 3000/AS 3000 programming through the TRACE GC Ultra or the
FOCUS GC, please refer to the following paragraphs respectively:
• Programming through the TRACE GC Ultra on page 70.
• Programming through the FOCUS GC on page 74.
Programming through the TRACE GC Ultra

This paragraph describes how to program and control the AI 3000/AS 3000
through the TRACE GC Ultra keypad.
Groups of samples can be automatically analyzed under different analytical

conditions by programming a sequence of samples. The sequence includes a set
of information for a group of samples and their position on the sample tray. The
sequence also specifies the method to be used to run each group of samples.
NOTE In an analytical method all the autoinjector/sampler functions can be programmed.

To develop an analytical method and activate a sample sequence, please refer to the
TRACE GC Ultra Operating Manual .
How to Configure the Autoinjector/Sampler

When the GC is switched on, the presence of the autoinjector/sampler and its
configuration is automatically acknowledged.
The type of configuration is displayed by pressing CONFIG and selecting

AUTOSAMPLER.
70 Operating Manual
Chapter 4
Control through the GC AI 3000/AS 3000 Control
The following non editable message is displayed according to the autoinjector/

sampler installed:
CONFIG AUTOSAMPLER
Sample Tray 105
for the AS 3000.
CONFIG AUTOSAMPLER
Sample Tray 8
for the AI 3000.
NOTE Should the autoinjector/sampler not be installed or correctly connected, the TRACE GC
Ultra will display the message NO AUTOSAMPLER INSTALLED.
Menu of the AI 3000/AS 3000

To set the parameters of the autoinjector/sampler method, press AUTOSAMPLER to
open the AUTOSAMPLER menu shown in table 4-1.
Table 4-1. Menu of the AI 3000/AS 3000

Menu Range Comments
AUTOSAMPLER This line displays the menu title.
Sample volume 0–5 µL This parameter allows to set the sample quantity to be
with increments injected.
of 0.1 µL
Sample rinses 0–15 This parameter allows to set the number of syringe pre-
washings with the sample.
Plunger 0–15 This parameter allows to set the number of plunger
strokes strokes to eliminate air bubbles forming during the sample
withdrawal.
Pre wash A, B, C, D, This parameter allows to select the vial, or combination of
solvent A+B, C+D two vials, containing the washing solvent. Press MODE/
TYPE to select the solvent vial to be used.
Operating Manual 71
Chapter 4
Table 4-1. Menu of the AI 3000/AS 3000 (Continued)

Menu Range Comments
Pre wash 0–15 This parameter allows to set the number of syringe pre-
cycles washings with the selected solvent.
Post wash A, B, C, D, This parameter allows to select the vial, or combination of
solvent A+B, C+D two vials, with the washing solvent. Press MODE/TYPE to
select the vial with the solvent to be used.
Post wash 0–15 This parameter specifies the number of syringe post-
cycles washings with the solvent selected.
Extended See table 4-2 This parameter allows to set optional injection parameters.
control Press MODE/TYPE to enter the submenu.
When no vial See table 4-3 This line shows the menu title.
abort
Extended Control Menu

In this menu it is possible to set how long the syringe needle must remain inside
the injector without injecting. This allows to avoid discriminations caused by
evaporation of the sample contained in the syringe needle. In this way the sample
is completely drawn into the syringe and the needle left inside the injector and
heated for a few seconds before injecting.
Table 4-2. Extended Control Menu

Menu Range Comments
EXTENDED CONTROL This line shows the menu title.
Viscous sample Yes, No This parameter defines the speed at which the sample
is drawn from the vial as a function of the sample
viscosity. Select NO (default value) if the sample has
low viscosity. Select YES if the sample has high
viscosity.
72 Operating Manual
Chapter 4
Table 4-2. Extended Control Menu (Continued)

Menu Range Comments
Sampl. depth Bottom, Center This parameter determines the penetration depth of
selectable in the the syringe needle into the vial.
submenu Press MODE/TYPE or ENTER to enter the submenu.
Selecting Bottom (default value) the needle goes
down to the vial bottom.
Selecting Center the needle goes down to half vial.
Inj. Depth Standard, This parameter determines the penetration depth of
Minimum the syringe needle into the injector.
selectable in the Press MODE/TYPE or ENTER to enter the submenu.
submenu
Selecting Standard (defualt value) the needle goes
down to the maximum depth allowed.
Selecting Minimum the needle enters the injector and
stops immediately beyond the septum (Cold Needle
Technique).
Pre dwell time 0–63 sec This parameter specifies how long the syringe needle
remains inside the injector without injecting (Hot
Needle Technique).
Post dwell time 0–63 sec This parameter specifies how long the syringe needle
remains inside the injector after injection .
When No Vial Abort Menu

Table 4-3. When No Vial Abort Menu
Menu Range Comments
ON MISSING AS VIAL This line shows the menu title.
Skip to next If this function is selected, the autoinjector/sampler skips a
missing vial and goes to the next one. The sample
sequence and the sample table are not affected.
Abort sequence If this function is selected, the sequence is aborted after a
missing vial.
Operating Manual 73
Chapter 4
Programming through the FOCUS GC

This paragraph describes how to program and control the AI 3000/AS 3000
through the FOCUS GC keypad.
The AS 3000 can automatically analyze groups of samples under different

analytical conditions when a sequence of samples is programmed. The sequence
includes a set of information for the group of samples and their position on the
sample tray. The sequence also specifies the method to be used to process each
group of samples. Refer to paragraph Sequence of Samples with the AS 3000 on
page 77.
How to Configure the Autoinjector/Sampler

When the GC is switched on, the presence and type of autoinjector/sampler
installed is automatically acknowledged.
To configure the autoinjector/sampler:
1. In Main Menu move the cursor until Others is displayed, then press ENTER.
2. Scroll to select Configuration, then press ENTER.
3. Scroll to Autosampler, then press ENTER.
The following message will be displayed according to the autoinjector/sampler

installed:
CONFIG AUTOSAMPLER
Syringe volume 10
Sample Tray 105
for the AS 3000.
CONFIG AUTOSAMPLER
Syringe volume 10
Sample Tray 8
74 Operating Manual
Chapter 4
for the AI 3000.
The parameter Syringe volume can be modified according to the syringe

volume, whereas the parameter Sample tray is not editable.
AI 3000/AS 3000 Menu

To set the parameters of the AI 3000/AS 3000 method, In Main Menu move the
cursor until Autosampler is displayed, then press ENTER to open the menu shown
in table 4-4.
Operating Manual 75
Chapter 4
Table 4-4. AI 3000/AS 3000 Menu

Menu Range Comments
AUTOSAMPLER This line shows the menu title.
Sample volume 0–5µL, 0–2.5µL This parameter allows to set the sample quantity to be
with increments injected.
of 0.1µL
Sample rinses 0–15 This parameter allows to set the number of syringe pre-
washings with the sample.
Plunger 0–15 This parameter allows to set the number of plunger
strokes strokes to eliminate air bubbles forming during the sample
withdrawal.
Pre wash A, B, C, D, This parameter allows to set the vial, or combination of
solvent A+B, C+D two vials, containing the washing solvent.
Pre wash 0–15 This parameter allows to set the number of syringe pre-
Post wash A, B, C, D, This parameter allows to set the vial, or combination of
solvent A+B, C+D two vials, containing the washing solvent.
Post wash 0–15 This parameter specifies the number of syringe post-
Viscous sample Yes, No This parameter sets the sample drawing speed from the
vial as a function of the sample viscosity.
Select No (default value) if the sample has low viscosity.
Select Yes when the sample has high viscosity.
Sample depth Bottom, Center This parameters sets the penetration depth of the syringe
selectable in the needle into the vial.
submenu If Bottom (defualt value) is selected, the needle goes
down to the vial bottom.
If Center is selected, the needle goes down to half vial.
76 Operating Manual
Chapter 4
Table 4-4. AI 3000/AS 3000 Menu (Continued)

Menu Range Comments
Inj. depth Standard, This parameter defines the penetration depth of the
Minimum syringe needle into the injector.
selectable in the If Standard (defualt value) is selected, the needle goes
submenu down to the maximum allowed depth.
If Minimum is selected, the needle enters the injector and
stops immediately beyond the septum (Cold Needle
Technique).
Pre dweel time 0–63 sec This parameter specifies how long the syringe needle
must remain in the injector before injection (Hot Needle
Technique).
Post dweel 0–63 sec This parameter specifies how long the syringe needle
time must remain inside the injector after injection.
Sequence of Samples with the AS 3000

To set the parameters to run a sequence of samples with the AS 3000, in Main
Menu move the cursor until Sequence is displayed, then press ENTER to open the
menu shown in Table 4-4.
Table 4-5. Sequence Menu with AS 3000

Menu Range Comments
SEQUENCE This line shows the menu title.
First sample 1 to 105 Indicates the first sample of the sequence.
Last sample 1 to 105 Indicates the last sample of the sequence.
Injection/vial 1 to 99 Indicates how many times the same sample has to be run.
When no vial See table 4-6
Start sequence Indicates the sequence start.
Stop Sequence Indicates the sequence end.
Operating Manual 77
Chapter 4
Table 4-6. When No Vial Abort Menu

Menu Range Comments
ON MISSING AS VIAL This line shows the menu title.
Skip to next When this item is selected, the autoinjector/sampler skips
a missing vial and goes to the next one. The sample
sequence and the sample table are not affected.
Abort sequence When this item is selected, the sequence will be aborted
after three missing vials.
78 Operating Manual
Chapter 4
Guidelines for Programming with Different Injectors AI 3000/AS 3000 Control
Guidelines for Programming with Different

Injectors
To get the best results from your AI/AS 3000, follow these guidelines when
programming the autosampler method according to the injector in use.
S/SL Inlet (Trace/Focus)

The autoinjector/sampler can inject in a hot S/SL inlet using two distinct needle
insertion modes: “Standard” and “Minimum”.
In the first mode the needle penetrates for almost its whole length and a dwell time
is programmed to provide needle heating. In the latter the needle penetrates for a
minimum depth and for the minimum time allowing to minimize contact of the
needle with the hot inlet.
These two needle insertion modes are conceived to optimize respectively the “Hot
Needle” and the “Cold Needle” injection techniques.
• Cold needle technique (Minimum depth mode) prevents boil-out from the
needle and lets the sample to enter the injector nearly entirely in liquid phase.
This injection technique requires the use of packed liner to stop the liquid run
(see S/SL Inlet with a Merlin Microseal™).
• Hot needle technique (Standard depth mode) requires a minimum pre-dwell
time to heat the needle (usually 3-5 seconds) to achieve a complete expulsion
of the sample from the needle in form of a spray. This technique permits the
use of empty liners.
The following Table 4-7 reports a summary of injection parameters and

conditions for the two SSL techniques. For your reference the type of liner and the
column insertion inside the inlet are also reported.
Operating Manual 79
Chapter 4
AI 3000/AS 3000 Control Guidelines for Programming with Different Injectors
Table 4-7. Summary of Injection Parameters and Conditions

Column AI 3000/AS 3000 Injection
Mode Technique Insertion Liner Type Packing of Liner Parameters
Hot Needle 40 mm Not required Standard Injection Depth
3-5 sec. Pre-Dwell Time

Split Tubular Liner 0 sec. Post-Dwell Time
Cold 40 mm (3 or 5 mm ID) Packing material Minimum Injection Depth
Needle at half height
Dwell Time are
automatically inhibited
Hot Needle 64 mm Not Required Standard Injection Depth

Splitless Tapered Liner 0 sec. Post-Dwell Time
Cold 50 mm (3 or 5 mm ID) Packing material Minimum Injection Depth
Needle at bottom
Dwell Time are
S/SL Nano- Cold 40 mm Double Tapered No Minimum Injection Depth
Volumes (*) Needle Liner
Dwell Time are
(*) Only with 0.5 µL syringe. For details, refer to the Nano-Volumes Kit for S/SL Inlet System User Guide.
Nano-Volumes Injections in S/SL Inlets

Injections of sample volumes below 0.1-0.2 µL in GC S/SL inlets is very
attractive when the dilution with a solvent is not desired and/or the main
component easily overloads the capillary column even at high split ratios.
Examples of such applications are analyses of essential oils or petroleum
fractions.
The reduction of the injection volume to the sub microliter range using a standard
autosampler, like the AI/AS 3000 unit, is achievable by exploiting the low
capacity syringe (0.5 µL) and the tapered liner provided in the Nano-Volumes Kit
for S/SL Inlet System. High accuracy and repeatability injecting nano-volumes are
achievable by exploiting the “Cold Needle” injection mode.
80 Operating Manual
Chapter 4
For injection volumes higher than 0.2-0.3 µL use a 5 (or 10) µL syringe and a
standard split or splitless liner (possibly packed with glass wool whenever you
inject in “Cold Needle” mode). In summary, use the following guideline to choose
the best syringe capacity for your injections with the AI/AS 3000 autosampler:
Table 4-8. Syringe Guideline for AI/AS 3000 Autosampler

Injection Volume Range Suggested Syringe (*) Notes
0.5 – 5.0 µL 10 µL Standard – general purpose
0.2 – 1.0 µL 5 µL ---
0.01 – 0.3 µL 0.5 µL Plunger-in-needle
(*) See your GC Spare Part Catalog for items part number.
OPERATING SEQUENCE
How to Pack a S/SL Liner
1. On your TRACE or FOCUS GC keypad enter the OVEN Menu and switch off
the oven temperature. This will avoid heating of the analytical column when
carrier is off.
2. Enter INLET Menu and switch off the inlet temperature.
3. Enter CARRIER Menu and switch off the carrier supply.
4. Remove the centering plate of the AI/AS3000 (if installed).
5. Wait till the injector has reached room temperature and by means of the outfit
tool unscrew the septum cap, then remove it together with the septum holder
and its plate.
6. Unscrew the liner holder and remove it.
7. Use tweezers to gently extract the liner from the injector.
Operating Manual 81
Chapter 4
8. Insert a little plug of packing material (i.e. deactivated glass wool) filling the
liner cavity about 5 mm in length. Push the plug till it reaches the position as
shown in picture.
9. Insert the liner in its location, then correctly close the injector and restore the
GC parameters.
Tubular Liner Tapered Liner
GLASS WOOL
S/SL Inlet with a Merlin Microseal™

This septum-less device offers an effective solution to get rid of traditional rubber
septa side effects (impurities released by excessive warming of septa). Since the
temperature around the microseal is lower than the rest of the injector, the most
suitable injections technique is the cold needle injection. That means: no dwell
time to warm up the needle. The Merlin adaptor also behaves as a spacer between
82 Operating Manual
Chapter 4
the needle entrance and the liner, so standard injection depth must be used to
fulfill correctly the injection.
NOTE In order to guarantee long-life performance of the Merlin Microseal™ it is recommended to

use syringes with 0.63 mm needle diameter and truncated cone shape on tip. Please refer
to the GC Spare Part Catalog for part number.
Column AI 3000/AS 3000 Injection

Mode Technique Insertion Liner Type Packing of Liner Parameters
Split 40 mm Packing material Standard Injection Depth
at half height
0 sec. Pre-Dwell Time
Cold Needle Tubular Liner 0 sec. Post-Dwell Time
Splitless 50 mm (3 or 5 mm ID) Packing material Standard Injection Depth
at bottom
0 sec. Pre-Dwell Time
0 sec. Post-Dwell Time
See the S/SL Inlet section for instruction on How to Pack a S/SL Liner
PTV Inlet
Since PTV is cold during sample introduction the syringe needle will not be
heated whenever standard or minimum depth is selected or dwell times are set.
However standard injection mode is advisable since the sample evaporation is
faster in the deeper part of the liner. Optional 1-2 seconds of dwell times will
guarantee stable flow condition after the needle introduction.
AI 3000/AS 3000 Injection

Injector Column Insertion Parameters
Programmed Temperature 30 mm Standard Injection Depth
Vaporizing Injector (PTV)
1-2 sec. Post-Dwell Time
Operating Manual 83
Chapter 4
PTV Inlet with a Merlin Microseal™

The above PTV conditions apply also when the Merlin Microseal is used.
NOTE In order to guarantee long-life performance of the Merlin microseal it is recommended to

use syringes with 0.63 mm needle diameter and truncated cone shape on tip. Please refer
to the spare part catalog for part number.
PKD and PPKD Inlets

For injection into packed column the standard insertion depth with 3-5 seconds of
Pre-dwell time is suggested.
Injector AI 3000/AS 3000 Injection Parameters

Packed Injector (PKD) Standard Injection Depth
Purged Packed Injector (PPKD)
0 sec. Post-Dwell Time
84 Operating Manual
Maintenance
5
This chapter provides guidelines for the maintenance of the AI 3000 / AS 3000.
Current Maintenance ............................................................................................ 86
Operating Sequences
Emptying of the Waste Container......................................................................... 86
Cleaning of the Sample Tray Accessory .............................................................. 86
External Cleaning of the Autoinjector/Sampler ................................................... 86
Replacement of the Syringe.................................................................................. 88
Cleaning of the Syringe ........................................................................................ 89
Preparation of a New Syringe............................................................................... 91
Operating Manual 85
Chapter 5
Maintenance Current Maintenance
Current Maintenance
The AI 3000/AS 3000 does not generally require maintenance, except emptying
of the waste container when full, cleaning of the sample tray and replacement of
the syringe. For any other operation, contact Thermo Electron Technical Service.
OPERATING SEQUENCE
Emptying of the Waste Container
1. If necessary, move the turret to have free access to the washing and waste tray.
2. Take out the container.
3. Remove the cap and empty the container.
4. Put on the cap again and reposition the container into its seat.
OPERATING SEQUENCE
Cleaning of the Sample Tray Accessory
The sample tray must be periodically cleaned.
1. Use a water and soap solution or a household non abrasive product. Dry using
a clean cloth.
OPERATING SEQUENCE
External Cleaning of the Autoinjector/Sampler
WARNING! The external cleaning must be performed with the instrument off and the power
cord disconnected. Avoid using solvents and spraying on electrical parts.
Before using dangerous substances (toxic, harmful etc.), read the hazard
86 Operating Manual
Chapter 5
Current Maintenance Maintenance
indications and information reported in the Safety Sheet supplied by the

manufacturer referring to the relevant CAS (Chemical Abstract Service) number.
1. Clean the instrument on the outside with a water and soap solution or with a
household non abrasive product. Pay special attention when cleaning the back
side of the sampling unit. Do not spray, but clean using a cloth imbued with
the same substance.
2. Dry with a clean cloth.
In case the operator suspects that any substance used for cleaning or to be
submitted to analysis may have entered the instrument, though it is very unlikely,
he/she shall immediately switch off the instrument and call the authorized
Technical Service for proper action.
Operating Manual 87
Chapter 5
OPERATING SEQUENCE
Replacement of the Syringe
The replacement of the syringe is a simple operation. However, it must be
performed with caution to avoid damages to the syringe needle and ensure an
optimal performance of the injection device.
NOTE 5 and 0.5 µL syringes are optionally available.
This operation is possible with the instrument either on or off.
Removal of the Syringe
1. Open the safety door of the turret.
NOTE If the instrument is on, power supply is immediately cut off.
2. Turn the syringe locking knob by 180° counterclockwise.
3. Gently take out the syringe plunger head and flange from their relevant
guides, then remove the syringe body from its seat.
4. Completely take out the syringe, paying attention while removing the needle
from the vial capture device.
Replacement of the Syringe
5. Take a new syringe.
6. Insert the syringe needle into the vial capture device.
7. Accomodate the syringe body into its seat, paying attention to simultaneously
introduce the syringe plunger flange and head into their relevant guides.
8. Lock the syringe by turning the lock wheel by 180° clockwise.
88 Operating Manual
Chapter 5
9. Close the safety door of the rotating turret.
NOTE If the instrument is on, when the door is closed the instrument automatically runs the
syringe zero routine.
If the instrument is off, the operation is performed when it is switched on during the self-test
routine.
OPERATING SEQUENCE
Cleaning of the Syringe
To get the best performance from the syringe installed in the autosampler and
increase the syringe lifetime here are some useful tips in order or cleaning impact:
1. If the sample is not viscous, program in the autosampler method one or more
rinse with sample to maintain the lubrication of the plunger.
2. Program in the autosampler method one or more post-injection wash cycle

with solvent.
3. If the sample is viscous, program in the autosampler method wash cycles of

extra solvents (e.g. A+B, where A is used to clean and B to rinse). The first
solvent should have a higher cleaning power than the second solvent. The
latter should be chemically similar to the sample scheduled for injection.
4. Perform an automated maintenance cycle every 100 injections (see Automated

Cleaning).
5. Perform a manual maintenance routine every 1000 injections (see Manual

Cleaning).
Automated Cleaning
The syringe maintenance cycle is always advisable after the injection of pure
samples, of undiluted mixes, of liquids containing solids in suspension or, at any
rate, after long sequence of analyses.
To do a cleaning cycle proceed as follows:
Operating Manual 89
Chapter 5
1. Fill a 4 mL vial with fresh solvent with high cleaning power (acetone is
recommended) and place it in the D position of the washing tray of the AI/
AS3000 autosampler.
2. From your PC, enter the Data System and then access the AI/AS3000 method
page. Select the following parameters:
• Pre-injection Solvent: D
• Pre-injection Cycles: 15
3. Enter the File Menu and click on Send Method to Sampler.
4. Enter the Command Menu and click on Start Clean Cycle.
5. If necessary, repeat Step 4 of this sequence.
Manual Cleaning
WARNING! This operating sequence is not applicable to the 0.5 µL syringe.

For details, refer to the Nano-Volumes Kit for S/SL Inlet System User Guide.
1. Remove the syringe as described in the operating sequence Replacement of

the Syringe.
2. Completely take out the syringe plunger and clean it gently using a soft cloth
or a paper tissue wetted with high cleaning power solvent (acetone is
recommended).
3. Reintroduce the syringe plunger and gently move it repeatedly along its whole
travel drawing an organic solvent (e.g. hexane).
4. Reinstall the syringe as described in the operating sequence Replacement of

the Syringe.
90 Operating Manual
Chapter 5
OPERATING SEQUENCE
Preparation of a New Syringe
This operating procedure is always advisable before using a new syringe with low
capacity (like a 5 or 0.5 µ L) or a new 10 µ L syringe with Teflon tip plunger. The
following procedure will allow to obtain the proper lubrication of the plunger.
1. Fill a 4 mL vial with fresh solvent (hexane is recommended) and place it in the
D position of the washing tray of the AI/AS3000 autosampler.
2. From your PC, enter the Data System and then access the AI/AS3000 method
page. Select the following parameters:
• Pre-injection Solvent: D
• Pre-injection Cycles: 15
3. Enter the File Menu and click on Send Method to Sampler.
4. Enter the Command Menu and click on Start Clean Cycle.
Operating Manual 91
Chapter 5
92 Operating Manual
“Space Saving” Installation
A
This type of installation is suggested to reduce the working area of your FOCUS
GC equipped with the AS 3000. This is applicable for example when two or more
FOCUS GCs are to be placed on the same workbench as shown in the following
figure.
1
3
1 2
4
2
1
4
A. Conventional Installation B. “Space Saving” Installation 1. Fixing Holes

2. Autoinjector/Sampler Support 3. AS 3000 4. Second FOCUS GC
Operating Manual 93
Appendix A
Proceed as follows:
corresponding fixing holes..
.
1. Fixing Holes
GC upper panel.
.
5. Continue the AS 3000 installation following the instruction reported in

Chapter 3.
94 Operating Manual
Installation on the Left Side
B
of the FOCUS GC MS Version
This type of installation may be carried out to reduce the working area of your
FOCUS GC MS Version. Refer to the following figure:
1 2
3 4
A. Conventional Installation B. “Left Side” Installation 1. Right Side Fixing Holes

2. Support Positioning 3. Left Side Fixing Holes 4. Support Positioning
Operating Manual 95
Appendix B
Proceed as follows:
1. Remove from the GC upper cover the two internal plastic caps covering the
.
1
1. Plastic Caps
GC upper panel.
.
1. Fixing Screws
96 Operating Manual
Appendix B
5. Continue the AI 3000 / AS 3000 installation following the instruction

reported in Chapter 3.
Operating Manual 97
Appendix B
98 Operating Manual
Customer
C
Communication
This appendix contains contact information for Thermo Electron offices
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survey to give us feedback on this manual and help us improve the quality of our
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How To Contact Us
Thermo Electron provides comprehensive technical assistance worldwide and is
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Operating Manual 99
Appendix C
Customer Communication How To Contact Us
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100 Operating Manual

Appendix C
How To Contact Us Customer Communication
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Operating Manual 101

Appendix C
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Manual: Operating Manual
Part No.: 317 093 92
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Appendix C

Glossary
This section contains an alphabetical list and descriptions of terms used in this manual. This also
includes abbreviations, acronyms, metric prefixes, and symbols.
A
A ampere
ac alternating current
ADC analog-to-digital converter
B
b bit
B byte (8 b)
baud rate data transmission speed in events per second
C
ºC Celsius
CIP Carriage and Insurance Paid To
cm centimeter
CPU central processing unit (of a computer)
CSE Customer Service Engineer
<Ctrl> control key on the terminal keyboard
D
d depth
DAC digital-to-analog converter
dc direct current
DS data system

Glossary
E
ECD Electron Capture Detector
EMC electromagnetic compatibility
ESD electrostatic discharge
F
ºF Fahrenheit
FID Flame Ionization Detector
FOB Free on Board
FPD Flame Photometric Detector
ft foot
G
g gram
GC gas chromatograph
GND electrical ground
H
h height
h hour
harmonic A high-frequency disturbance that appears as distortion of the
distortion fundamental sine wave.
HV high voltage
Hz hertz (cycles per second)
I
IEC International Electrotechnical Commission

Glossary
impulse See transient

in. inch
I/O input/output
K
k kilo (103 or 1024)
K Kelvin
kg kilogram
kPa kilopascal
L
l length
l liter
lb pound
LED light-emitting diode
M
m meter (or milli [10-3])
M mega (106)
µ micro (10-6)
min minute
mL milliliter
mm millimeter
m/z mass-to-charge ratio
N
n nano (10-9)

Glossary
NPD Nitrogen Phosphorous Detector
O
Ω ohm
P
p pico (10-12 )
Pa pascal
PCB printed circuit board
PID Photo Ionization Detector
PN part number
psi pounds per square inch
R
RAM random access memory
<Return> <Return> key on the keyboard
RF radio frequency
ROM read-only memory
RS-232 industry standard for serial communications
S
s second
sag See surge
slow average A gradual, long-term change in average RMS voltage level,
with typical durations greater than 2 s.
surge A sudden change in average RMS voltage level, with typical
duration between 50 µs and 2 s.

Glossary
T
TCD Thermal Conductivity Detector
transient A brief voltage surge of up to several thousand volts, with a
duration of less than 50 µs.
V
V volt
V ac volts, alternating current
V dc volts, direct current
VGA Video Graphics Array
W
w Width
W Watt
NOTE The symbol for a compound unit that is a quotient (for example, degrees Celsius per
minute or grams per liter) is written with a negative exponent with the denominator.
For example:
°C min-1 instead of °C/min
g L-1 instead of g/L

Glossary

Index
A Configurations of the Autosampler 20
Abort sequence 78 Control
AI 3000 through the GC 70
Assembling of the Autosampler Support on the GC Control from PC 62
right side 46, 47, 49 Current Maintenance 86
Assembling of the Syringe 52 Customer Communication 97
Electrical Connections 52 Cycles 65, 66
Installation of the Sampling Unit (Left
Configuration) 52 D
Installation of the Sampling Unit (Right Dimensions 23
Configuration) 50
Instrument Startup 53 E
AI 3000 Autoinjector Edit Menu 67
Configuration 20 Electrical Connections 52, 57
AI 3000 Autosampler 17 Electrical Requirements 40
AI 3000/AS 3000 Autosampler Error Messages 69
Menu 71 Exit AS2000 Parameters 67
AS 3000 Extended control 72
Electrical Connections 57 Extended Control Menu 72
Installation of the Sampling Unit (Left External interface 23
Configuration) 56
F
Installation of the sampling unit (Right
Configuration(Right Configuration) 54 File Menu 66
Instrument Startup 57 First sample 77
AS 3000 Autosampler 17 Flushing solvents 23
Configuration 21 FOCUS GC MS Version
Assembling of the Syringe 52 Installation on the Left Side 93
Autosampler
G
Configuration 20
Control 61 Get Method from Gemini 67
from PC 62 H
Support 41 How to Carry the Sampling Unit 40
Autosamplers
How to Configure the Autosampler 70, 74
Setup 74
How To Contact Us 97
B How to Install the AI 3000 on the GC 50
How to Install the Autosampler AS 3000 on the
Base 18, 26
GC 54
C How to Lift the Sampling Unit 40
Centering Plate 18, 37
I
Classification of the Instrument 16
Inj. depth 77
Cleaning of the Syringe 89
Command Menu 68 Injection Assembly 18, 29
Injection depth
Commands
64
Writing Conventions xii

Index
Injection Section 64 Open 66

Injection/vial 77 Open Recent... 66
Installation 21, 39 Print 67
AI 3000 on the GC 50 Save (Save as...) 66
AS 3000 on the GC 54 Save As Default 67
Left Configuration 22 Send Method to Sampler 67
Preliminary Information 40 Menu Help 68
Right configuration 21 Menus
Who Performs the 40 Description 66
Installazione del campionatore AS 2000 sul GC 40 Messages 69
Instrument Error 69
Classification 16 Status 69
Instrument Markings xii Movements of the Turret 32
Instrument Symbols xii
Introduction 15 N
Nano-Volumes Injections 80
L
Last sample 77 O
Open Method 66
M
Maintenance 85 P
Cleaning of the SampleTray Accessory 86 Plunger stroker 63
Emptying of the Waste Container 86 Plunger strokes 63, 71, 76
External Cleaning of the Autosampler 86 Post dweel time 77
Replacement of the Syringe 88 Post Injection 65
Manual Post Injection Cycles 66
Conventions Used x Post Injection Solvent 65
Overview ix Post wash cycles 72, 76
Menu Post wash solvent 72, 76
Descrizione 66 Post-inj dwell time 64
Extended Control 72 Post-inj dwell time (s) 64
File 66 Power supply 23
When No Vial Abort 73 Pre dweel time 77
Menu Command 68 Pre Injection 65
Force Autoinjector to Stand-by 68 Pre Injection Cycles 65
Inject Single Sample 68 Pre Injection Solvent 65
Start Clean Cycle 68 Pre wash cycles 72, 76
Menu Edit 67 Pre wash solvent 71, 76
View Audit Trail 67 Pre-inj dwell time 64
Menu File Pre-inj dwell time (s) 64
Close 67 Print 67
Get Method from Gemini 67 Programmability 23
Get Method from Sampler 67 Programming
Load from Default 67 through the FOCUS GC 74
New 66 through the TRACE GC 70

Index
R T
Reader Survey 101 Technical Specifications 23
Removal of the Syringe 88 Turret 28
Replacement of the Syringe 88 Types of syringes 23
Revolving turret 18
U
S User Interface 19, 62
S/SL Liner
How to Pack 81 V
Safety Information 16 Vial Capture Device 30
Sample 65 Vials capacity 23
Sample depth 76 View Audit Trail 67
Sample Rinses 65 Viscous sample 63, 76
Sample rinses 71, 76 Volumes programming 23
Sample Tray 18
8-Position 35 W
Assembly Washes Section 64
105-position 35 Washing and waste tray 18
Sample tray capacity 23 Washing Tary 33
Sample Trays 35 Washing Tray 18
Sample volume 63, 71, 76 Waste container capacity 23
Sample volume (ml) 63 Waste Tray 18, 33
Sampler Support 19 Weight 23
Samples Rinses When no vial 77
65 When no vial abort 72
Sampling depth in vial Working Procedures 79
63 PKD Inlet 84
Sampling Section 63 PPKD Inlet 84
Sampling Unit 18, 25 PTV Inlet 83
Rear 34 PTV Inlet with a Merlin Microseal™ 84
Save Method 66 S/SL Inlet (Trace/Focus) 79
Sequence of Samples with the AS 3000 S/SL Inlet with a Merlin Microseal™ 82
Autosampler 77
Skip to next 78
Solvent 65
Start sequence 77
Status LED 38
Status Messages 69
Stop Sequence 77
Syringe 30
Cleaning 89
Removal 88
Replacement 88
Syringe carriage assembly 18

Index

List of Figures
AI 3000 and AS 3000 Diagram .......................................................................................................... 17
AI 3000 Autoinjector ......................................................................................................................... 20
AS 3000 Autosampler ........................................................................................................................ 21
Right Configuration ........................................................................................................................... 22
Left Configuration ............................................................................................................................. 22
Sections of the Base ........................................................................................................................... 26
The Turret ...................................................................................................................................... 28
Injection Assembly ............................................................................................................................ 29
Vial Capture Device ........................................................................................................................... 30
ìSyringe Housing ................................................................................................................................ 31
Washing and Waste Tray ................................................................................................................... 33
Rear of the Sampling Unit .................................................................................................................34
8-Postiion Sample Tray ..................................................................................................................... 35
Supporting Plate and 105-Position Sample Tray ............................................................................... 36
Centering Plate ................................................................................................................................... 37
Status LED ...................................................................................................................................... 38
How to lift and carry the Sampling Unit............................................................................................. 41
Support for the installation of the AI 3000/AS 3000 on the TRACE GC .......................................... 42
Bracket for the installation of the AI 3000/AS 3000 Support ............................................................ 43
Support for the installation of the AI 3000/AS 3000 on the FOCUS GC .......................................... 44
Installation of the Spacer inside the GC ............................................................................................. 46
Installation of the Syringe (1) ............................................................................................................. 59
Installation of the Syringe (2) ............................................................................................................. 60
User Interface for AI 3000/AS 3000 .................................................................................................. 62
List of Tables
Technical Specifications .................................................................................................................... 23
Menu of the AI 3000/AS 3000 .......................................................................................................... 71
Extended Control Menu ..................................................................................................................... 72
When No Vial Abort Menu ............................................................................................................... 73
AI 3000/AS 3000 Menu ..................................................................................................................... 76
Sequence Menu with AS 3000 ........................................................................................................... 77
When No Vial Abort Menu .............................................................................................................. 78
Summary of Injection Parameters and Conditions .............................................................................80
Syringe Guideline for AI/AS 3000 Autosampler ............................................................................... 81
Operating Sequences
How to Install the Autoinjector/Sampler Support on the TRACE GC Ultra ..................................... 45
How to Install Autoinjector/Sampler Support on the FOCUS GC .................................................... 49
How to Install the AI 3000 on the GC ............................................................................................... 50
How to Install the AS 3000 on the GC .............................................................................................. 54
How to Install the Syringe ................................................................................................................. 58
How to Pack a S/SL Liner .................................................................................................................. 81
Emptying of the Waste Container....................................................................................................... 86
Cleaning of the Sample Tray Accessory ............................................................................................ 86
External Cleaning of the Autoinjector/Sampler ................................................................................. 86
Replacement of the Syringe................................................................................................................ 88
Cleaning of the Syringe ..................................................................................................................... 89
Preparation of a New Syringe............................................................................................................. 91

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HOME AI 3000 / AS 3000

3000 Series A u tomatic Sampling System

Printing History: First edition, released January 2002

have been carried out by authorized personnel and if:

Thermo Electron S.p.A.

Operating Manual iii

Sample Trays ........................................................................................................................... 35

When No Vial Abort Menu............................................................................ 73

Operating Manual vii

viii Operating Manual

This manual is organized as follows:

Chapter 1, Introduction, provides a basic overview of the features and options of

Chapter 2, Sampling Unit, provides a description of the components of the

Chapter 3, Installation, contains the instructions for the installation of the

Chapter 4, AI 3000/AS 3000 Control, provides the information to control the

Chapter 5, Maintenance, provides guidelines for the maintenance of the

Appendix A, “Space Saving” Installation, contains the installation sequence of

Appendix B, Installation on the Left Side of the FOCUS GC MS Version, contains

Appendix C, Customer Communication, contains contact information for Thermo

Conventions Used in This Manual

Bold Bold text indicates names of windows, menus, dialog boxes,

This symbol alerts you to important information related to the

This symbol alerts you to an action or procedure that, if

This symbol indicates danger from hazardous chemicals.

This symbol indicates danger from high temperature surfaces

This symbol indicates a fire hazard.

This symbol indicates an explosion hazard.

This symbol indicates a toxic hazard.

This symbol indicates the presence of flammable materials.

This symbol indicates the presence of radioactive material.

This symbol indicates an operation or procedure that must

This symbol indicates all metal objects, such as watches,

This symbol indicates an eye hazard. Eye protection must be

This symbol indicates the user must wear a protective screen

This symbol indicates the user must wear protective shoes

This symbol indicates the user must wear protective clothing

This symbol indicates the user must wear gloves when

Instrument Markings and Symbols

Both direct and alternating current

Three-phase alternating current

Protective conductor terminal

Frame or chassis terminal

Equipment protected throughout by DOUBLE

Caution, hot surface

Caution (refer to accompanying documents)

Out-position of a bistable push control

Classification of the Instrument

The AI 3000 / AS 3000

1. Safety door 2. Turret

Washing and Waste Tray

For further information, please refer to:

Configurations of the 3000 Series Automatic

The autoinjector/sampler is available in the following options:

AI 3000 Autoinjector Configuration

Figure 1-2. AI 3000 Autoinjector

As a function of the configuration of installation, right or left, the support will be

AS 3000 Autosampler Configuration

Figure 1-3. AS 3000 Autosampler

Figura 1-4. Right Configuration

Figure 1-5. Left Configuration

WARNING! If an existing AI 3000 Autoinjector will be upgraded to AS 3000 Autosampler, it is

Front section Right side section

Left side section Rear section

Figure 2-1. Sections of the Base