Abstract

The Dengue Haemorrhagic Fever Viruses are a group of ssRNA flaviviruses that can cause

haemorrhagic fever in humans, often mediated by cross-serotype immune response. Extant

treatment is solely supportive and is designed to counter the effects of any physiological

shock that may accompany the haemorrhage. It is therefore vital that the growth of the

virus be targeted to prevent a cross serotype immune response from occurring.

Several gene silencing methods have been discovered of late, allowing target specific gene

silencing with a remarkable degree of precision, and these offer a route to therapy that is

devoid of the limitations faced by extant chemotherapeutic paradigms. I will be discussing

these technologies in depth in the course of the paper and pointing out reasons of

significance that bolster the case for gene silencing to be used to counter pathogens in place

of traditional chemotherapy.

There are bioinformatics tools that enable the implementation of data gained from studies

of the silencing technologies available towards the stated objective of achieving highly site

and target specific silencing, either using enzyme independent mechanisms, as in the case of

RNA Interference, and enzyme independent methods, such as the use of phosphorothioate

oligomers, locked nucleic acid oligomers and morpholino antisense oligomers.

I discuss the nature of the genomes of all the four dengue viruses using information

retrieved from databases followed by phylogenetic analysis to outline a strategy for

identifying targets for therapeutic silencing using the methods listed above. I then apply

design tools/algorithms to the insights gained through genome analysis to produce a raw

collection of silencing targets and silencing oligomers, which I will refer to as the raw

silenceome from this point onwards, to serve as drug leads for future wet lab driven

research efforts into evolving a therapeutic mechanism to combat what looks like an

emerging healthcare headache in several countries round the world.

I also propose an integrated diagnosis-treatment process based on extant biotechnological

tools and also hypothesize about the nature of the progression of the dengue viral infection

and what implications that may have on the way the treatment of the disease is

approached.
Figure 1: In Silico Design Workflow
Dengue Haemorrhagic Fever: A Clinical and Epidemological Perspective.

Dengue is a flaviviral infection transmitted through Aedes spp mosquitoes [1], in recent

years, it has emerged as a major healthcare problem in the tropics [2] and notably so in

India [3]. The infection is mediated by four different serotypes of the virus [3], , it has also

been noted that early immune activation of a T Cell response by the virus may be linked to a

worse prognosis, often involving haemorrhage [4] and this immune response usually takes

the form of a cross reactive T-Cell response [5]

The disease itself can manifest in two forms, Dengue fever, and Dengue Haemorrhagic

Fever. The former is characterised by high fever (upto 105’F) and associated symptoms such

as frontal headaches, retro-orbital pain, body aches, nausea, vomiting, weakness and body

rashes. Other symptoms include anorexia, mild cases of sore throat, altered sensation of

taste and respiratory symptoms may be reported, attributable to the presence of

simultaneously occurring secondary infections.

 Figure 2: Increase in Incidence of Dengue Fever (WHO,2008)

The other form, which is associated with higher mortality, is called Dengue Haemorrhagic Fever

(DHF), and is usually constrained to children under fifteen years of age and may occur in adults, and

is characteristically marked by symptoms that appear as the fever goes into remission, and these

include signs of plasma leakage, severe reduction in platelet counts, and other haemocytological

parameters that are indicative of vascular leak syndrome

The occurrence of scattered petichiae, and venal bleeding, and Gastrointestinal tract haemorrhage is

also associated with more severe cases of DHF and it is the propensity of the disease to lead to

physiological shock is one of the most dangerous features of the disease. [6]

Figure 3: Geographical Spread of Dengue Fever and Dengue Haemorrhagic Fever (WHO,2008)

In the next section, I will be outlining the foundational concepts used to develop the In-silico model

that I employed during the course of my work.

Evolving a therapeutic paradigm: Foundational Concepts.

As discussed in the preceding section, there is extant evidence available in the literature to

suggest that…

[1] DHF Requires a cross reactive immune response to occur.

[2] Early Immune Activation often is correlated with a worse prognosis, and therefore it

would be optimal to prevent this from happening, since immune activation is triggered by

the virus, I hypothesize that this could be achieved by blocking viral replication at an early

stage, it is in this regard that I propose a silencing based approach.

[3] Haemorrhage is often followed by a remission in the fever [7] on the basis of which I

formulate a hypothesis, which, , before it can be used to develop further therapeutic

strategies, must accrue evidential support pertaining to the testable predictions it makes.

This hypothesis and the possible therapeutic implications resulting there from will be

discussed later in the dissertation.

In the next section, I will provide a brief outline of the rationale behind the choice of
technologies that are proposed to be employed in the development of the silenceomic
approach and details of the genomes of the four viral serotypes and how they affected the
in-silico development of the silenceomic panels that provide a launching pad to investigate
therapies pertaining to point [2].
The Dengue Virus Genome & Implications for Panel Design.

The virus is an RNA flavivirus without a DNA intermediate and on the whole comprises of a

polyprotein precursor that produces both structural and non-structural proteins [8], it is in

light of this that I propose carrying out genome wide targeting to obtain a silenceomic

panel.

Figure 4: Dengue Virus Genome Structure (adapted from Pang et al)

This would immediately lead to the question of whether it would be possible to generate a

broad spectrum panel, allowing silencing by means of a single antisense molecule common

to all four serotypes, this would require that virus specific (i.e, no off target reactivity with

human transcripts) sequences be found that were totally conserved between all four

serotypes, for a stretch of at least nineteen nucleotides for phosphorothioate oligomers

(which are 19 to 25 base pairs long) and twenty-five to twenty-eight bases for dicer

substrate RNA Interference. (siRNA for which is 25-28 base pairs long).

A search for such conserved sequences was carried out using the built in Multiple Sequence

Alignment function in CLC Sequence Viewer 6 (CLC Bio) using whole genome sequences of

the serotypes retrieved from NCBI’s Genbank, accession details of which follow.

Virus Serotype Genbank Accession Number

Type 1 U88536.1
Type 2 U87411.1
Type 3 AY099336.1
Type 4 AF326825.1

The complete sequences can be found as a data supplement for reference and verification.

The search resulted in no such conserved sequences meeting the required criterion for all

four serotypes, and there was substantially enough variation amongst the serotypes in

terms of genome sequences to warrant the design of separate panels for each of the four

serotypes, usually such diversity would have caused fatal problems in terms of therapeutic

choice and delivery protocols, but for reasons that will be discussed when describing the

proposed therapeutic model there appears to be a workable solution. The alignments in

question have been provided as a data supplement in both .PDF and ClustalW2/ClustalX2

Alignment (.aln) formats.

 Figure 5: Evolutionary relationships between the serotypes of DFV/DHFV, produced using TreeView & ClustalX2
Alignments.

Employed Silencing Strategies : A Primer.

Phosphorothioate oligomers are modified polynucleotide strands wherein the

phosphodiester bonds are modified to carry a sulphur atom instead of an oxygen atom, thus

conferring resistance to nucleases and a sufficiently high melting temperature value (Tm) to

be able to duplex with target sequences, serving as a mechanism for gene silencing, they

can range from 19-25 nucleotides in length [9], a few examples of successful gene

knockdowns involving phosphorothioate nucleic acid oligomers include regulation of HIV

replication [10] , blocking cell proliferation by targeting cell signalling pathways [11] and

working as antitumour agents [12] and affecting cell adhesion [13] to mention a few

examples.
These conclusively demonstrate that phosphorothioate oligomers are capable of achieving

potent gene silencing.

The other strategy for which a silenceomic panel is being developed in this case is RNA

interference, and dsRNAi (or Dicer Substrate RNAi) in particular, which achieves gene

silencing by triggering RISC induced cleavage of RNA that is complementary to a double

stranded version of the same, and this has increasingly become a promising therapeutic

strategy of late [14] , some notable observations of gene knockdown using this strategy

include silencing Ti Plasmid activity in plants [15] Gene knockdown in mouse brains [16]

and VEGF knockdown in Retinoblastoma as a means of antitumour therapy [17] & in

preventing neurodegeneration during conditions like ataxia [18]. Recent advances in the

field include the employment of a more advanced method for RNA Interference, which lasts

longer and can have 100 fold more potent silencing activity, called Dicer Substrate RNAi or

dsRNAi [19] and it is using algorithms that use design parameters optimized for this form of

RNAi that I have developed the RNAi silenceome for the four dengue flaviviruses.
In Silico Construction of Phosphorothioate Oligomer Panel.

Integrated DNA Technologies SciTools Suite [20] was used for this purpose and the first

10,000 bases were arbitrarily chosen for identifying sites unique to the viral genome while

not having any off-target reactivity to human gene transcripts. All phosphorothioate

enneadecamers/icosamers were the parameters chosen for oligomer designs and 20

binding sites each were identified in the genome of each virus, followed by the in-silico

derivation of the corresponding silencing sequences and an estimation of the properties

thereof. The sequences that were designed are available as a data supplement, both

digitally and as an attachment in print after the dissertation.

Figure 6:Silencing using translation blocking antisense oligomers (adapted from Corey, 2007)
In Silico Construction of dsRNAi Panel.

The same set of tools was used for this purpose [20] and the algorithm parameters were set

to dsRNAi 27-mer sequences, similar design measures to prevent off target interactions with

human transcripts were identified and corresponding dsRNAi sequences were designed. The

sequences that were designed are again available as a digital supplement. The exercise was

again repeated for the first 10,000 bases of each viral genome.

Figure 7: Dicer Substrate RNA Interference (Dore'-Savard et al, 2008)

Towards an Integrated Workflow: The Value of Diagnosis.

The fact that conserved targets were not available for panel design and development is

unlikely to pose problems, not least because of the development of cheap PCR cyclers that

perform with reliable accuracy [21] and the presence of an extant Reverse Transcriptase

PCR based diagnostic method for extremely rapid and efficient serotype identification. [22]

This now paves the way for the direct implementation of treatment options by way of virus

like particles or liposomal transfection in an effort to block viral replication effectively, the

significance of the body of work so far can only be ascertained after the necessary wet lab

tests are carried out, which may be aided by the availability of an extant mouse model for

Dengue Haemorrhagic Fever [23]

A non-silenceomic approach: A hypothesis.

I also present another hypothesis which, although outside the remit of the current work,

provide a second line of therapy should a silenceomic approach fail to achieve its objectives,

taking note of the fact that remission of the fever in Dengue is closely associated with the

onset of haemorrhage, as has been cited before in this paper, and is also associated with a

cross reactive T Cell Immune response, I hypothesize that the sudden shift may be

associated with a drop in viral load as the fever goes into remission, to be followed by a

change in the reaction dynamics between the virus, cross-reactive T cells, and the body’s

own tissues. If this hypothesis is confirmed I am of the opinion that it will provide yet more

therapeutic targets compared to what is already available, which is practically next to

nothing since care is totally supportive at this juncture. [24]

Conclusions

The availability of computational methods combined with a rich collection of information

from various bioinformatics databases actively facilitated the in silico development of a

library of molecules that will make for optimal lead compounds in the search for a

therapeutic strategy against Dengue Fever and Dengue Haemorrhagic Fever, the workflow

that was devised as part of the project should also prove suitable for the development of

therapeutic strategies using silencing against other pathogens, most notably viruses, and

further forays into other diseases using the same approach may very well be on the anvil.

However, it must still be recognized that in silico approaches to development have an

inherent limitation in that they still require external validation, my work should therefore

rightly be seen not as a complete solution, but as a vital contribution in the pursuit of such a

solution, with much of the initial technical and experimental headaches in generating a

library of candidate compounds et cetera being avoided by the implementation of the

apposite computational strategies, to sum it all up, I am of the opinion that this work could

characterise a drug development programme that is based on newly emerging silencing

based therapeutic strategies.

Acknowledgements.

I would like to thank Smt.Lakshmi Chilangi of In-Gene Life Technologies, Mysore , for her

assistance and guidance during the completion of the research presented in this

dissertation, I would also like to thank Dr.Ravinarayana C.S for providing inputs from a

clinical perspective with respect to Dengue Fever and Dengue Haemorrhagic Fever and

everybody in the computational biology, gene silencing, virology and epidemiology

community whose work in diverse areas ranging from the development of tools to the

production of high quality data to the development of novel therapeutic approaches has

made my work possible.

List of Supplements.

A. Whole Genome Data

B. Multiple Sequence Alignment

C. Phosphorothioate Oligomer Panel

D. Dicer Substrate siRNA Panel

E. Toolbox (List of tools used , brief descriptions & links)

References

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[3] Concurrent infections by all four dengue virus serotypes during an outbreak of dengue in

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[7] Principles and practise of clinical virology, Zuckerman et al, John Wiley and Sons, 2009,

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[8] A Structural Perspective of the Flavivirus Life Cycle, Suchetana Mukhopadhyay et al,

Nature Reviews | Microbiology, Volume 3 | January 2005 | 13

[9] Physicochemical properties of phospborothioate oligodeoxynucleotides, C.A.Stein et al.

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[10] Regulation of viral expression of human immunodeficiency virus in vitro by an antisense

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Matsukura et al,PNAS June 1, 1989 vol. 86 no. 11 4244-4248

[11] Downregulation of c-myc expression by antisense oligonucleotides inhibits proliferation

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[12] Antitumor Effects of Antisense Phosphorothioate c-myc Oligodeoxynucleotides: a

Question of Mechanism, C. A. Stein* , Journal of the National Cancer Institute, Vol. 88, No.

7, April 3, 1996
[13] Multiple mechanisms may contribute to the cellular anti-adhesive effects of

phosphorothioate oligodeoxynucleotides, Z Khaled et al, 1. Nucl. Acids Res. (1996) 24 (4):

737-745. doi: 10.1093/nar/24.4.737

[14] RNAi: A novel antisense technology and its therapeutic potential, Anne Dallas et al,

Med Sci Monit, 2006; 12(4): RA67-74

[15] RNAi-mediated oncogene silencing confers resistance to crown gall tumorigenesis,

Matthew A. Escobar et al, www.pnas.org/cgi/doi/10.1073/pnas.241276898

[16] Local gene knockdown in the brain using viral-mediated RNA interference, Jonathan D

Hommel et al, Nature Medicine 9, 1539 - 1544 (2003)

[17] VEGF-Targeted RNA Interference Suppresses Angiogenesis and Tumor Growth of

Retinoblastoma, Jia RB et al, Ophthalmic Res 2007;39:108-115 (DOI: 10.1159/000099247)

[18] RNAi suppresses polyglutamine-induced neurodegeneration in a model of

spinocerebellar ataxia, Haibin Xia et al, Nature Medicine 10, 816 - 820 (2004)
[19] Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy, Dong-Ho Kim et

al, Nature Biotechnology 23, 222 - 226 (2004)

[20] IDT SciTools: a suite for analysis and design of nucleic acid oligomers, Richard Owczarzy

et al, Nucl. Acids Res. (2008) 36 (suppl 2): W163-W169. doi: 10.1093/nar/gkn198

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[22] Rapid Detection and Typing of Dengue Viruses from Clinical Samples by Using Reverse

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[23] New Mouse Model for Dengue Virus Vaccine Testing, Alison J. Johnson* and John T.

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Image References & Sources.

Figure 1: Self Created Workflow Diagram, using Cmap Tools.

Figure 2: http://www.who.int/csr/disease/dengue/impact/en/

Figure 3: http://www.who.int/csr/disease/dengue/impact/en/

Figure 4: Pang et al, Development of Dengue virus type 2 replicons capable of prolonged
expression in host cells, BMC Microbiology 2001, 1:18

Figure 5: Self generated phylogenetic tree using Treeview.

Figure 6: RNA learns from antisense, David R Corey, Nature Chemical Biology 3, 8 - 11 (2007)
doi:10.1038/nchembio0107-8

Figure 7: Central Delivery of Dicer-substrate siRNA: A Direct Application for Pain Research,
Louis Dore'-Savard et al, Molecular Therapy (2008) 16 7, 1331–1339
doi:10.1038/mt.2008.98
Whole Genome Sequence
Data
Raw nucleotide sequences of the genomes of all
four Dengue virus serotypes
Dengue Virus Type 1.
>gi|1854038|gb|U88536.1|DVU88536 Dengue virus type 1 clone 45AZ5, complete genome

AGTTGTTAGTCTACGTGGACCGACAAGAACAGTTTCGAATCGGAAGCTTGCTTAACGTAGTTCTAACAGT

TTTTTATTAGAGAGCAGATCTCTGATGAACAACCAACGGAAAAAGACGGGTCGACCGTCTTTCAATATGC

TGAAACGCGCGAGAAACCGCGTGTCAACTGTTTCACAGTTGGCGAAGAGATTCTCAAAAGGATTGCTTTC

AGGCCAAGGACCCATGAAATTGGTGATGGCTTTTATAGCATTCCTAAGATTTCTAGCCATACCTCCAACA

GCAGGAATTTTGGCTAGATGGGGCTCATTCAAGAAGAATGGAGCGATCAAAGTGTTACGGGGTTTCAAGA

AAGAAATCTCAAACATGTTGAACATAATGAACAGGAGGAAAAGATCTGTGACCATGCTCCTCATGCTGCT

GCCCACAGCCCTGGCGTTCCATCTGACCACCCGAGGGGGAGAGCCGCACATGATAGTTAGCAAGCAGGAA

AGAGGAAAATCACTTTTGTTTAAGACCTCTGCAGGTGTCAACATGTGCACCCTTATTGCAATGGATTTGG

GAGAGTTATGTGAGGACACAATGACCTACAAATGCCCCCGGATCACTGAGACGGAACCAGATGACGTTGA

CTGTTGGTGCAATGCCACGGAGACATGGGTGACCTATGGAACATGTTCTCAAACTGGTGAACACCGACGA

GACAAACGTTCCGTCGCACTGGCACCACACGTAGGGCTTGGTCTAGAAACAAGAACCGAAACGTGGATGT

CCTCTGAAGGCGCTTGGAAACAAATACAAAAAGTGGAGACCTGGGCTCTGAGACACCCAGGATTCACGGT

GATAGCCCTTTTTCTAGCACATGCCATAGGAACATCCATCACCCAGAAAGGGATCATTTTTATTTTGCTG

ATGCTGGTAACTCCATCCATGGCCATGCGGTGCGTGGGAATAGGCAACAGAGACTTCGTGGAAGGACTGT

CAGGAGCTACGTGGGTGGATGTGGTACTGGAGCATGGAAGTTGCGTCACTACCATGGCAAAAGACAAACC

AACACTGGACATTGAACTCTTGAAGACGGAGGTCACAAACCCTGCCGTCCTGCGCAAACTGTGCATTGAA

GCTAAAATATCAAACACCACCACCGATTCGAGATGTCCAACACAAGGAGAAGCCACGCTGGTGGAAGAAC

AGGACACGAACTTTGTGTGTCGACGAACGTTCGTGGACAGAGGCTGGGGCAATGGTTGTGGGCTATTCGG

AAAAGGTAGCTTAATAACGTGTGCTAAGTTTAAGTGTGTGACAAAACTGGAAGGAAAGATAGTCCAATAT

GAAAACTTAAAATATTCAGTGATAGTCACCGTACACACTGGAGACCAGCACCAAGTTGGAAATGAGACCA

CAGAACATGGAACAACTGCAACCATAACACCTCAAGCTCCCACGTCGGAAATACAGCTGACAGACTACGG

AGCTCTAACATTGGATTGTTCACCTAGAACAGGGCTAGACTTTAATGAGATGGTGTTGTTGACAATGAAA

AAAAAATCATGGCTCGTCCACAAACAATGGTTTCTAGACTTACCACTGCCTTGGACCTCGGGGGCTTCAA

CATCCCAAGAGACTTGGAATAGACAAGACTTGCTGGTCACATTTAAGACAGCTCATGCAAAAAAGCAGGA
AGTAGTCGTACTAGGATCACAAGAAGGAGCAATGCACACTGCGTTGACTGGAGCGACAGAAATCCAAACG

TCTGGAACGACAACAATTTTTGCAGGACACCTGAAATGCAGATTAAAAATGGATAAACTGATTTTAAAAG

GGATGTCATATGTAATGTGCACAGGGTCATTCAAGTTAGAGAAGGAAGTGGCTGAGACCCAGCATGGAAC

TGTTCTAGTGCAGGTTAAATACGAAGGAACAGATGCACCATGCAAGATCCCCTTCTCGTCCCAAGATGAG

AAGGGAGTAACCCAGAATGGGAGATTGATAACAGCCAACCCCATAGTCACTGACAAAGAAAAACCAGTCA

ACATTGAAGCGGAGCCACCTTTTGGTGAGAGCTACATTGTGGTAGGAGCAGGTGAAAAAGCTTTGAAACT

AAGCTGGTTCAAGAAGGGAAGCAGTATAGGGAAAATGTTTGAAGCAACTGCCCGTGGAGCACGAAGGATG

GCCATCCTGGGAGACACTGCATGGGACTTCGGTTCTATAGGAGGGGTGTTCACGTCTGTGGGAAAACTGA

TACACCAGATTTTTGGGACTGCGTATGGAGTTTTGTTCAGCGGTGTTTCTTGGACCATGAAGATAGGAAT

AGGGATTCTGCTGACATGGCTAGGATTAAACTCAAGGAGCACGTCCCTTTCAATGACGTGTATCGCAGTT

GGCATGGTCACACTGTACCTAGGAGTCATGGTTCAGGCGGACTCGGGATGTGTAATCAACTGGAAAGGCA

GAGAACTCAAATGTGGAAGCGGCATTTTTGTCACCAATGAAGTCCACACCTGGACAGAGCAATATAAATT

CCAGGCCGACTCCCCTAAGAGACTATCAGCGGCCATTGGGAAGGCATGGGAGGAGGGTGTGTGTGGAATT

CGATCAGCCACTCGTCTCGAGAACATCATGTGGAAGCAAATATCAAATGAATTAAACCACATCTTACTTG

AAAATGACATGAAATTTACAGTGGTCGTAGGAGACGTTAGTGGAATCTTGGCCCAAGGAAAGAAAATGAT

TAGGCCACAACCCATGGAACACAAATACTCGTGGAAAAGCTGGGGAAAAGCCAAAATCATAGGAGCAGAT

GTACAGAATACCACCTTCATCATCGACGGCCCAAACACCCCAGAATGCCCTGATAACCAAAGAGCATGGA

ACATTTGGGAAGTTGAAGACTATGGATTTGGAATTTTCACGACAAACATATGGTTGAAATTGCGTGACTC

CTACACTCAAGTGTGTGACCACCGGCTAATGTCAGCTGCCATCAAGGATAGCAAAGCAGTCCATGCTGAC

ATGGGGTACTGGATAGAAAGTGAAAAGAACGAGACTTGGAAGTTGGCAAGAGCCTCCTTCATAGAAGTTA

AGACATGCATCTGGCCAAAATCCCACACTCTATGGAGCAATGGAGTCCTGGAAAGTGAGATGATAATCCC

AAAGATATATGGAGGACCAATATCTCAGCACAACTACAGACCAGGATATTTCACACAAACAGCAGGGCCG

TGGCACTTGGGCAAGTTAGAACTAGATTTTGATTTATGTGAAGGTACCACTGTTGTTGTGGATGAACATT

GTGGAAATCGAGGACCATCTCTTAGAACCACAACAGTCACAGGAAAGACAATCCATGAATGGTGCTGTAG

ATCTTGCACGTTACCCCCCCTACGTTTCAAAGGAGAAGACGGGTGCTGGTACGGCATGGAAATCAGACCA

GTCAAGGAGAAGGAAGAGAACCTAGTTAAGTCAATGGTCTCTGCAGGGTCAGGAGAAGTGGACAGTTTTT

CACTAGGACTGCTATGCATATCAATAATGATCGAAGAGGTAATGAGATCCAGATGGAGCAGAAAAATGCT
GATGACTGGAACATTGGCTGTGTTCCTCCTTCTCACAATGGGACAATTGACATGGAATGATCTGATCAGG

CTATGTATCATGGTTGGAGCCAACGCTTCAGACAAGATGGGGATGGGAACAACGTACCTAGCTTTGATGG

CCACTTTCAGAATGAGACCAATGTTCGCAGTCGGGCTACTGTTTCGCAGATTAACATCTAGAGAAGTTCT

TCTTCTTACAGTTGGATTGAGTCTGGTGGCATCTGTAGAACTACCAAATTCCTTAGAGGAGCTAGGGGAT

GGACTTGCAATGGGCATCATGATGTTGAAATTACTGACTGATTTTCAGTCACATCAGCTATGGGCTACCT

TGCTGTCTTTAACATTTGTCAAAACAACTTTTTCATTGCACTATGCATGGAAGACAATGGCTATGATACT

GTCAATTGTATCTCTCTTCCCTTTATGCCTGTCCACGACTTCTCAAAAAACAACATGGCTTCCGGTGTTG

CTGGGATCTCTTGGATGCAAACCACTAACCATGTTTCTTATAACAGAAAACAAAATCTGGGGAAGGAAAA

GCTGGCCTCTCAATGAAGGAATTATGGCTGTTGGAATAGTTAGCATTCTTCTAAGTTCACTTCTCAAGAA

TGATGTGCCACTAGCTGGCCCACTAATAGCTGGAGGCATGCTAATAGCATGTTATGTCATATCTGGAAGC

TCGGCCGATTTATCACTGGAGAAAGCGGCTGAGGTCTCCTGGGAAGAAGAAGCAGAACACTCTGGTGCCT

CACACAACATACTAGTGGAGGTCCAAGATGATGGAACCATGAAGATAAAGGATGAAGAGAGAGATGACAC

ACTCACCATTCTCCTCAAAGCAACTCTGCTAGCAATCTCAGGGGTATACCCAATGTCAATACCGGCGACC

CTCTTTGTGTGGTATTTTTGGCAGAAAAAGAAACAGAGATCAGGAGTGCTATGGGACACACCCAGCCCTC

CAGAAGTGGAAAGAGCAGTCCTTGATGATGGCATTTATAGAATTCTCCAAAGAGGATTGTTGGGCAGGTC

TCAAGTAGGAGTAGGAGTTTTTCAAGAAGGCGTGTTCCACACAATGTGGCACGTCACCAGGGGAGCTGTC

CTCATGTACCAAGGGAAGAGACTGGAACCAAGTTGGGCCAGTGTCAAAAAAGACTTGATCTCATATGGAG

GAGGTTGGAGGTTTCAAGGATCCTGGAACGCGGGAGAAGAAGTGCAGGTGATTGCTGTTGAACCGGGGAA

GAACCCCAAAAATGTACAGACAGCGCCGGGTACCTTCAAGACCCCTGAAGGCGAAGTTGGAGCCATAGCT

CTAGACTTTAAACCCGGCACATCTGGATCTCCTATCGTGAACAGAGAGGGAAAAATAGTAGGTCTTTATG

GAAATGGAGTGGTGACAACAAGTGGTACCTACGTCAGTGCCATAGCTCAAGCTAAAGCATCACAAGAAGG

GCCTCTACCAGAGATTGAGGACGAGGTGTTTAGGAAAAGAAACTTAACAATAATGGACCTACATCCAGGA

TCGGGAAAAACAAGAAGATACCTTCCAGCCATAGTCCGTGAGGCCATAAAAAGAAAGCTGCGCACGCTAG

TCTTAGCTCCCACAAGAGTTGTCGCTTCTGAAATGGCAGAGGCGCTCAAGGGAATGCCAATAAGGTATCA

GACAACAGCAGTGAAGAGTGAACACACGGGAAAGGAGATAGTTGACCTTATGTGTCACGCCACTTTCACT

ATGCGTCTCCTGTCTCCTGTGAGAGTTCCCAATTATAATATGATTATCATGGATGAAGCACATTTTACCG

ATCCAGCCAGCATAGCAGCCAGAGGGTATATCTCAACCCGAGTGGGTATGGGTGAAGCAGCTGCGATTTT
CATGACAGCCACTCCCCCCGGATCGGTGGAGGCCTTTCCACAGAGCAATGCAGTTATCCAAGATGAGGAA

AGAGACATTCCTGAAAGATCATGGAACTCAGGCTATGACTGGATCACTGATTTCCCAGGTAAAACAGTCT

GGTTTGTTCCAAGCATCAAATCAGGAAATGACATTGCCAACTGTTTAAGAAAGAATGGGAAACGGGTGGT

CCAATTGAGCAGAAAAACTTTTGACACTGAGTACCAGAAAACAAAAAATAACGACTGGGACTATGTTGTC

ACAACAGACATATCCGAAATGGGAGCAAACTTCCGAGCCGACAGGGTAATAGACCCGAGGCGGTGCCTGA

AACCGGTAATACTAAAAGATGGCCCAGAGCGTGTCATTCTAGCCGGACCGATGCCAGTGACTGTGGCTAG

CGCCGCCCAGAGGAGAGGAAGAATTGGAAGGAACCAAAATAAGGAAGGCGATCAGTATATTTACATGGGA

CAGCCTCTAAACAATGATGAGGACCACGCCCATTGGACAGAAGCAAAAATGCTCCTTGACAACATAAACA

CACCAGAAGGGATTATCCCAGCCCTCTTTGAGCCGGAGAGAGAAAAGAGTGCAGCAATAGACGGGGAATA

CAGACTACGGGGTGAAGCGAGGAAAACGTTCGTGGAGCTCATGAGAAGAGGAGATCTACCTGTCTGGCTA

TCCTACAAAGTTGCCTCAGAAGGCTTCCAGTACTCCGACAGAAGGTGGTGCTTTGATGGGGAAAGGAACA

ACCAGGTGTTGGAGGAGAACATGGACGTGGAGATCTGGACAAAAGAAGGAGAAAGAAAGAAACTACGACC

CCGCTGGCTGGATGCCAGAACATACTCTGACCCACTGGCTCTGCGCGAATTCAAAGAGTTCGCAGCAGGA

AGAAGAAGCGTCTCAGGTGACCTAATATTAGAAATAGGGAAACTTCCACAACATTTAACGCAAAGGGCCC

AGAACGCCTTGGACAATCTGGTTATGTTGCACAACTCTGAACAAGGAGGAAAAGCCTATAGACACGCCAT

GGAAGAACTACCAGACACCATAGAAACGTTAATGCTCCTAGCTTTGATAGCTGTGCTGACTGGTGGAGTG

ACGTTGTTCTTCCTATCAGGAAGGGGTCTAGGAAAAACATCCATTGGCCTACTCTGCGTGATTGCCTCAA

GTGCACTGTTATGGATGGCCAGTGTGGAACCCCATTGGATAGCGGCCTCTATCATACTGGAGTTCTTTCT

GATGGTGTTGCTTATTCCAGAGCCGGACAGACAGCGCACTCCACAAGACAACCAGCTAGCATACGTGGTG

ATAGGTCTGTTATTCATGATATTGACAGTGGCAGCCAATGAGATGGGATTACTGGAAACCACAAAGAAGG

ACCTGGGGATTGGTCATGCAGCTGCTGAAAACCACCATCATGCTGCAATGCTGGACGTAGACCTACATCC

AGCTTCAGCCTGGACTCTCTATGCAGTGGCCACAACAATTATCACTCCCATGATGAGACACACAATTGAA

AACACAACGGCAAATATTTCCCTGACAGCTATTGCAAACCAGGCAGCTATATTGATGGGACTTGACAAGG

GATGGCCAATATCAAAGATGGACATAGGAGTTCCACTTCTCGCCTTGGGGTGCTATTCTCAGGTGAACCC

GCTGACGCTGACAGCGGCGGTATTGATGCTAGTGGCTCATTATGCCATAATTGGACCCGGACTGCAAGCA

AAAGCTACTAGAGAAGCTCAAAAAAGGACAGCAGCCGGAATAATGAAAAACCCAACTGTCGACGGGATCG

TTGCAATAGATTTGGACCCTGTGGTTTACGATGCAAAATTTGAAAAACAGCTAGGCCAAATAATGTTGTT
GATACTTTGCACATCACAGATCCTCCTGATGCGGACCACATGGGCCTTGTGTGAATCCATCACACTAGCC

ACTGGACCTCTGACTACGCTTTGGGAGGGATCTCCAGGAAAATTCTGGAACACCACGATAGCGGTGTCCA

TGGCAAACATTTTTAGGGGAAGTTATCTAGCAGGAGCAGGTCTGGCCTTTTCATTAATGAAATCTCTAGG

AGGAGGTAGGAGAGGCACGGGAGCCCAAGGGGAAACACTGGGAGAAAAATGGAAAAGACAGCTAAACCAA

TTGAGCAAGTCAGAATTCAACACTTACAAAAGGAGTGGGATTATAGAGGTGGATAGATCTGAAGCCAAAG

AGGGGTTAAAAAGAGGAGAAACGACTAAACACGCAGTGTCGAGAGGAACGGCCAAACTGAGGTGGTTTGT

GGAGAGGAACCTTGTGAAACCAGAAGGGAAAGTCATAGACCTCGGTTGTGGAAGAGGTGGCTGGTCATAT

TATTGCGCTGGGCTGAAGAAAGTCACAGAAGTGAAAGGATACACGAAAGGAGGACCTGGACATGAGGAAC

CAATCCCAATGGCAACCTATGGATGGAACCTAGTAAAGCTATACTCCGGGAAAGATGTATTCTTTACACC

ACCTGAGAAATGTGACACCCTCTTGTGTGATATTGGTGAGTCCTCTCCGAACCCAACTATAGAAGAAGGA

AGAACGTTACGTGTTCTAAAGATGGTGGAACCATGGCTCAGAGGAAACCAATTTTGCATAAAAATTCTAA

ATCCCTATATGCCGAGTGTGGTAGAAACTTTGGAGCAAATGCAAAGAAAACATGGAGGAATGCTAGTGCG

AAATCCACTCTCAAGAAACTCCACTCATGAAATGTACTGGGTTTCATGTGGAACAGGAAACATTGTGTCA

GCAGTAAACATGACATCTAGAATGCTGCTAAATCGATTCACAATGGCTCACAGGAAGCCAACATATGAAA

GAGACGTGGACTTAGGCGCTGGAACAAGACATGTGGCAGTAGAACCAGAGGTGGCCAACCTAGATATCAT

TGGCCAGAGGATAGAGAATATAAAAAATGAACACAAATCAACATGGCATTATGATGAGGACAATCCATAC

AAAACATGGGCCTATCATGGATCATATGAGGTCAAGCCATCAGGATCAGCCTCATCCATGGTCAATGGTG

TGGTGAGACTGCTAACCAAACCATGGGATGTCATTCCCATGGTCACACAAATAGCCATGACTGACACCAC

ACCCTTTGGACAACAGAGGGTGTTTAAAGAGAAAGTTGACACGCGTACACCAAAAGCGAAACGAGGCACA

GCACAAATTATGGAGGTGACAGCCAGGTGGTTATGGGGTTTTCTCTCTAGAAACAAAAAACCCAGAATCT

GCACAAGAGAGGAGTTCACAAGAAAAGTCAGGTCAAACGCAGCTATTGGAGCAGTGTTCGTTGATGAAAA

TCAATGGAACTCAGCAAAAGAGGCAGTGGAAGATGAACGGTTCTGGGACCTTGTGCACAGAGAGAGGGAG

CTTCATAAACAAGGAAAATGTGCCACGTGTGTCTACAACATGATGGGAAAGAGAGAGAAAAAATTAGGAG

AGTTCGGAAAGGCAAAAGGAAGTCGCGCAATATGGTACATGTGGTTGGGAGCGCGCTTTTTAGAGTTTGA

AGCCCTTGGTTTCATGAATGAAGATCACTGGTTCAGCAGAGAGAATTCACTCAGTGGAGTGGAAGGAGAA

GGACTCCACAAACTTGGATACATACTCAGAGACATATCAAAGATTCCAGGGGGAAATATGTATGCAGATG

ACACAGCCGGATGGGACACAAGAATAACAGAGGATGATCTTCAGAATGAGGCCAAAATCACTGACATCAT
GGAACCTGAACATGCCCTATTGGCCACGTCAATCTTTAAGCTAACCTACCAAAACAAGGTAGTAAGGGTG

CAGAGACCAGCGAAAAATGGAACCGTGATGGATGTCATATCCAGACGTGACCAGAGAGGAAGTGGACAGG

TTGGAACCTATGGCTTAAACACCTTCACCAACATGGAGGCCCAACTAATAAGACAAATGGAGTCTGAGGG

AATCTTTTCACCCAGCGAATTGGAAACCCCAAATCTAGCCGAAAGAGTCCTCGACTGGTTGAAAAAACAT

GGCACCGAGAGGCTGAAAAGAATGGCAATCAGTGGAGATGACTGTGTGGTGAAACCAATCGATGACAGAT

TTGCAACAGCCTTAACAGCTTTGAATGACATGGGAAAGGTAAGAAAAGACATACCGCAATGGGAACCTTC

AAAAGGATGGAATGATTGGCAACAAGTGCCTTTCTGTTCACACCATTTCCACCAGCTGATTATGAAGGAT

GGGAGGGAGATAGTGGTGCCATGCCGCAACCAAGATGAACTTGTAGGTAGGGCCAGAGTATCACAAGGCG

CCGGATGGAGCTTGAGAGAAACTGCATGCCTAGGCAAGTCATATGCACAAATGTGGCAGCTGATGTACTT

CCACAGGAGAGACTTGAGATTAGCGGCTAATGCTATCTGTTCAGCCGTTCCAGTTGATTGGGTCCCAACC

AGCCGCACCACCTGGTCGATCCATGCCCACCATCAATGGATGACAACAGAAGACATGTTGTCAGTGTGGA

ATAGGGTTTGGATAGAGGAAAACCCATGGATGGAGGACAAGACTCATGTGTCCAGTTGGGAAGACGTTCC

ATACCTAGGAAAAAGGGAAGATCAATGGTGTGGTTCCCTAATAGGCTTAACAGCACGAGCCACCTGGGCC

ACCAACATACAAGTGGCCATAAACCAAGTGAGAAGGCTCATTGGGAATGAGAATTATCTAGACTTCATGA

CATCAATGAAGAGATTCAAAAACGAGAGTGATCCCGAAGGGGCACTCTGGTAAGCCAACTCATTCACAAA

ATAAAGGAAAATAAAAAATCAAACAAGGCAAGAAGTCAGGCCGGATTAAGCCATAGCACGGTAAGAGCTA

TGCTGCCTGTGAGCCCCGTCCAAGGACGTAAAATGAAGTCAGGCCGAAAGCCACGGTTCGAGCAAGCCGT

GCTGCCTGTAGCTCCATCGTGGGGATGTAAAAACCCGGGAGGCTGCAAACCATGGAAGCTGTACGCATGG

GGTAGCAGACTAGTGGTTAGAGGAGACCCCTCCCAAGACACAACGCAGCAGCGGGGCCCAACACCAGGGG

AAGCTGTACCCTGGTGGTAAGGACTAGAGGTTAGAGGAGACCCCCCGCACAACAACAAACAGCATATTGA

CGCTGGGAGAGACCAGAGATCCTGCTGTCTCTACAGCATCATTCCAGGCACAGAACGCCAAAAAATGGAA

TGGTGCTGTTGAATCAACAGGTTCT.
Dengue Virus Type 2.

>gi|2155257|gb|U87411.1| Dengue virus type 2 (strain 16681) polyprotein mRNA, complete cds

AGTTGTTAGTCTACGTGGACCGACAAAGACAGATTCTTTGAGGGAGCTAAGCTCAACGTAGTTCTAACAG

TTTTTTAATTAGAGAGCAGATCTCTGATGAATAACCAACGGAAAAAGGCGAAAAACACGCCTTTCAATAT

GCTGAAACGCGAGAGAAACCGCGTGTCGACTGTGCAACAGCTGACAAAGAGATTCTCACTTGGAATGCTG

CAGGGACGAGGACCATTAAAACTGTTCATGGCCCTGGTGGCGTTCCTTCGTTTCCTAACAATCCCACCAA

CAGCAGGGATATTGAAGAGATGGGGAACAATTAAAAAATCAAAAGCTATTAATGTTTTGAGAGGGTTCAG

GAAAGAGATTGGAAGGATGCTGAACATCTTGAATAGGAGACGCAGATCTGCAGGCATGATCATTATGCTG

ATTCCAACAGTGATGGCGTTCCATTTAACCACACGTAACGGAGAACCACACATGATCGTCAGCAGACAAG

AGAAAGGGAAAAGTCTTCTGTTTAAAACAGAGGATGGCGTGAACATGTGTACCCTCATGGCCATGGACCT

TGGTGAATTGTGTGAAGACACAATCACGTACAAGTGTCCCCTTCTCAGGCAGAATGAGCCAGAAGACATA

GACTGTTGGTGCAACTCTACGTCCACGTGGGTAACTTATGGGACGTGTACCACCATGGGAGAACATAGAA

GAGAAAAAAGATCAGTGGCACTCGTTCCACATGTGGGAATGGGACTGGAGACACGAACTGAAACATGGAT

GTCATCAGAAGGGGCCTGGAAACATGTCCAGAGAATTGAAACTTGGATCTTGAGACATCCAGGCTTCACC

ATGATGGCAGCAATCCTGGCATACACCATAGGAACGACACATTTCCAAAGAGCCCTGATTTTCATCTTAC

TGACAGCTGTCACTCCTTCAATGACAATGCGTTGCATAGGAATGTCAAATAGAGACTTTGTGGAAGGGGT

TTCAGGAGGAAGCTGGGTTGACATAGTCTTAGAACATGGAAGCTGTGTGACGACGATGGCAAAAAACAAA

CCAACATTGGATTTTGAACTGATAAAAACAGAAGCCAAACAGCCTGCCACCCTAAGGAAGTACTGTATAG

AGGCAAAGCTAACCAACACAACAACAGAATCTCGCTGCCCAACACAAGGGGAACCCAGCCTAAATGAAGA

GCAGGACAAAAGGTTCGTCTGCAAACACTCCATGGTAGACAGAGGATGGGGAAATGGATGTGGACTATTT

GGAAAGGGAGGCATTGTGACCTGTGCTATGTTCAGATGCAAAAAGAACATGGAAGGAAAAGTTGTGCAAC

CAGAAAACTTGGAATACACCATTGTGATAACACCTCACTCAGGGGAAGAGCATGCAGTCGGAAATGACAC

AGGAAAACATGGCAAGGAAATCAAAATAACACCACAGAGTTCCATCACAGAAGCAGAATTGACAGGTTAT

GGCACTGTCACAATGGAGTGCTCTCCAAGAACGGGCCTCGACTTCAATGAGATGGTGTTGCTGCAGATGG

AAAATAAAGCTTGGCTGGTGCACAGGCAATGGTTCCTAGACCTGCCGTTACCATGGTTGCCCGGAGCGGA

CACACAAGGGTCAAATTGGATACAGAAAGAGACATTGGTCACTTTCAAAAATCCCCATGCGAAGAAACAG

GATGTTGTTGTTTTAGGATCCCAAGAAGGGGCCATGCACACAGCACTTACAGGGGCCACAGAAATCCAAA
TGTCATCAGGAAACTTACTCTTCACAGGACATCTCAAGTGCAGGCTGAGAATGGACAAGCTACAGCTCAA

AGGAATGTCATACTCTATGTGCACAGGAAAGTTTAAAGTTGTGAAGGAAATAGCAGAAACACAACATGGA

ACAATAGTTATCAGAGTGCAATATGAAGGGGACGGCTCTCCATGCAAGATCCCTTTTGAGATAATGGATT

TGGAAAAAAGACATGTCTTAGGTCGCCTGATTACAGTCAACCCAATTGTGACAGAAAAAGATAGCCCAGT

CAACATAGAAGCAGAACCTCCATTCGGAGACAGCTACATCATCATAGGAGTAGAGCCGGGACAACTGAAG

CTCAACTGGTTTAAGAAAGGAAGTTCTATCGGCCAAATGTTTGAGACAACAATGAGGGGGGCGAAGAGAA

TGGCCATTTTAGGTGACACAGCCTGGGATTTTGGATCCTTGGGAGGAGTGTTTACATCTATAGGAAAGGC

TCTCCACCAAGTCTTTGGAGCAATCTATGGAGCTGCCTTCAGTGGGGTTTCATGGACTATGAAAATCCTC

ATAGGAGTCATTATCACATGGATAGGAATGAATTCACGCAGCACCTCACTGTCTGTGACACTAGTATTGG

TGGGAATTGTGACACTGTATTTGGGAGTCATGGTGCAGGCCGATAGTGGTTGCGTTGTGAGCTGGAAAAA

CAAAGAACTGAAATGTGGCAGTGGGATTTTCATCACAGACAACGTGCACACATGGACAGAACAATACAAG

TTCCAACCAGAATCCCCTTCAAAACTAGCTTCAGCTATCCAGAAAGCCCATGAAGAGGGCATTTGTGGAA

TCCGCTCAGTAACAAGACTGGAGAATCTGATGTGGAAACAAATAACACCAGAATTGAATCACATTCTATC

AGAAAATGAGGTGAAGTTAACTATTATGACAGGAGACATCAAAGGAATCATGCAGGCAGGAAAACGATCT

CTGCGGCCTCAGCCCACTGAGCTGAAGTATTCATGGAAAACATGGGGCAAAGCAAAAATGCTCTCTACAG

AGTCTCATAACCAGACCTTTCTCATTGATGGCCCCGAAACAGCAGAATGCCCCAACACAAATAGAGCTTG

GAATTCGTTGGAAGTTGAAGACTATGGCTTTGGAGTATTCACCACCAATATATGGCTAAAATTGAAAGAA

AAACAGGATGTATTCTGCGACTCAAAACTCATGTCAGCGGCCATAAAAGACAACAGAGCCGTCCATGCCG

ATATGGGTTATTGGATAGAAAGTGCACTCAATGACACATGGAAGATAGAGAAAGCCTCTTTCATTGAAGT

TAAAAACTGCCACTGGCCAAAATCACACACCCTCTGGAGCAATGGAGTGCTAGAAAGTGAGATGATAATT

CCAAAGAATCTCGCTGGACCAGTGTCTCAACACAACTATAGACCAGGCTACCATACACAAATAACAGGAC

CATGGCATCTAGGTAAGCTTGAGATGGACTTTGATTTCTGTGATGGAACAACAGTGGTAGTGACTGAGGA

CTGCGGAAATAGAGGACCCTCTTTGAGAACAACCACTGCCTCTGGAAAACTCATAACAGAATGGTGCTGC

CGATCTTGCACATTACCACCGCTAAGATACAGAGGTGAGGATGGGTGCTGGTACGGGATGGAAATCAGAC

CATTGAAGGAGAAAGAAGAGAATTTGGTCAACTCCTTGGTCACAGCTGGACATGGGCAGGTCGACAACTT

TTCACTAGGAGTCTTGGGAATGGCATTGTTCCTGGAGGAAATGCTTAGGACCCGAGTAGGAACGAAACAT

GCAATACTACTAGTTGCAGTTTCTTTTGTGACATTGATCACAGGGAACATGTCCTTTAGAGACCTGGGAA
GAGTGATGGTTATGGTAGGCGCCACTATGACGGATGACATAGGTATGGGCGTGACTTATCTTGCCCTACT

AGCAGCCTTCAAAGTCAGACCAACTTTTGCAGCTGGACTACTCTTGAGAAAGCTGACCTCCAAGGAATTG

ATGATGACTACTATAGGAATTGTACTCCTCTCCCAGAGCACCATACCAGAGACCATTCTTGAGTTGACTG

ATGCGTTAGCCTTAGGCATGATGGTCCTCAAAATGGTGAGAAATATGGAAAAGTATCAATTGGCAGTGAC

TATCATGGCTATCTTGTGCGTCCCAAACGCAGTGATATTACAAAACGCATGGAAAGTGAGTTGCACAATA

TTGGCAGTGGTGTCCGTTTCCCCACTGCTCTTAACATCCTCACAGCAAAAAACAGATTGGATACCATTAG

CATTGACGATCAAAGGTCTCAATCCAACAGCTATTTTTCTAACAACCCTCTCAAGAACCAGCAAGAAAAG

GAGCTGGCCATTAAATGAGGCTATCATGGCAGTCGGGATGGTGAGCATTTTAGCCAGTTCTCTCCTAAAA

AATGATATTCCCATGACAGGACCATTAGTGGCTGGAGGGCTCCTCACTGTGTGCTACGTGCTCACTGGAC

GATCGGCCGATTTGGAACTGGAGAGAGCAGCCGATGTCAAATGGGAAGACCAGGCAGAGATATCAGGAAG

CAGTCCAATCCTGTCAATAACAATATCAGAAGATGGTAGCATGTCGATAAAAAATGAAGAGGAAGAACAA

ACACTGACCATACTCATTAGAACAGGATTGCTGGTGATCTCAGGACTTTTTCCTGTATCAATACCAATCA

CGGCAGCAGCATGGTACCTGTGGGAAGTGAAGAAACAACGGGCCGGAGTATTGTGGGATGTTCCTTCACC

CCCACCCATGGGAAAGGCTGAACTGGAAGATGGAGCCTATAGAATTAAGCAAAAAGGGATTCTTGGATAT

TCCCAGATCGGAGCCGGAGTTTACAAAGAAGGAACATTCCATACAATGTGGCATGTCACACGTGGCGCTG

TTCTAATGCATAAAGGAAAGAGGATTGAACCATCATGGGCGGACGTCAAGAAAGACCTAATATCATATGG

AGGAGGCTGGAAGTTAGAAGGAGAATGGAAGGAAGGAGAAGAAGTCCAGGTATTGGCACTGGAGCCTGGA

AAAAATCCAAGAGCCGTCCAAACGAAACCTGGTCTTTTCAAAACCAACGCCGGAACAATAGGTGCTGTAT

CTCTGGACTTTTCTCCTGGAACGTCAGGATCTCCAATTATCGACAAAAAAGGAAAAGTTGTGGGTCTTTA

TGGTAATGGTGTTGTTACAAGGAGTGGAGCATATGTGAGTGCTATAGCCCAGACTGAAAAAAGCATTGAA

GACAACCCAGAGATCGAAGATGACATTTTCCGAAAGAGAAGACTGACCATCATGGACCTCCACCCAGGAG

CGGGAAAGACGAAGAGATACCTTCCGGCCATAGTCAGAGAAGCTATAAAACGGGGTTTGAGAACATTAAT

CTTGGCCCCCACTAGAGTTGTGGCAGCTGAAATGGAGGAAGCCCTTAGAGGACTTCCAATAAGATACCAG

ACCCCAGCCATCAGAGCTGAGCACACCGGGCGGGAGATTGTGGACCTAATGTGTCATGCCACATTTACCA

TGAGGCTGCTATCACCAGTTAGAGTGCCAAACTACAACCTGATTATCATGGACGAAGCCCATTTCACAGA

CCCAGCAAGTATAGCAGCTAGAGGATACATCTCAACTCGAGTGGAGATGGGTGAGGCAGCTGGGATTTTT

ATGACAGCCACTCCCCCGGGAAGCAGAGACCCATTTCCTCAGAGCAATGCACCAATCATAGATGAAGAAA
GAGAAATCCCTGAACGTTCGTGGAATTCCGGACATGAATGGGTCACGGATTTTAAAGGGAAGACTGTTTG

GTTCGTTCCAAGTATAAAAGCAGGAAATGATATAGCAGCTTGCCTGAGGAAAAATGGAAAGAAAGTGATA

CAACTCAGTAGGAAGACCTTTGATTCTGAGTATGTCAAGACTAGAACCAATGATTGGGACTTCGTGGTTA

CAACTGACATTTCAGAAATGGGTGCCAATTTCAAGGCTGAGAGGGTTATAGACCCCAGACGCTGCATGAA

ACCAGTCATACTAACAGATGGTGAAGAGCGGGTGATTCTGGCAGGACCTATGCCAGTGACCCACTCTAGT

GCAGCACAAAGAAGAGGGAGAATAGGAAGAAATCCAAAAAATGAGAATGACCAGTACATATACATGGGGG

AACCTCTGGAAAATGATGAAGACTGTGCACACTGGAAAGAAGCTAAAATGCTCCTAGATAACATCAACAC

GCCAGAAGGAATCATTCCTAGCATGTTCGAACCAGAGCGTGAAAAGGTGGATGCCATTGATGGCGAATAC

CGCTTGAGAGGAGAAGCAAGGAAAACCTTTGTAGACTTAATGAGAAGAGGAGACCTACCAGTCTGGTTGG

CCTACAGAGTGGCAGCTGAAGGCATCAACTACGCAGACAGAAGGTGGTGTTTTGATGGAGTCAAGAACAA

CCAAATCCTAGAAGAAAACGTGGAAGTTGAAATCTGGACAAAAGAAGGGGAAAGGAAGAAATTGAAACCC

AGATGGTTGGATGCTAGGATCTATTCTGACCCACTGGCGCTAAAAGAATTTAAGGAATTTGCAGCCGGAA

GAAAGTCTCTGACCCTGAACCTAATCACAGAAATGGGTAGGCTCCCAACCTTCATGACTCAGAAGGCAAG

AGACGCACTGGACAACTTAGCAGTGCTGCACACGGCTGAGGCAGGTGGAAGGGCGTACAACCATGCTCTC

AGTGAACTGCCGGAGACCCTGGAGACATTGCTTTTACTGACACTTCTGGCTACAGTCACGGGAGGGATCT

TTTTATTCTTGATGAGCGGAAGGGGCATAGGGAAGATGACCCTGGGAATGTGCTGCATAATCACGGCTAG

CATCCTCCTATGGTACGCACAAATACAGCCACACTGGATAGCAGCTTCAATAATACTGGAGTTTTTTCTC

ATAGTTTTGCTTATTCCAGAACCTGAAAAACAGAGAACACCCCAAGACAACCAACTGACCTACGTTGTCA

TAGCCATCCTCACAGTGGTGGCCGCAACCATGGCAAACGAGATGGGTTTCCTAGAAAAAACGAAGAAAGA

TCTCGGATTGGGAAGCATTGCAACCCAGCAACCCGAGAGCAACATCCTGGACATAGATCTACGTCCTGCA

TCAGCATGGACGCTGTATGCCGTGGCCACAACATTTGTTACACCAATGTTGAGACATAGCATTGAAAATT

CCTCAGTGAATGTGTCCCTAACAGCTATAGCCAACCAAGCCACAGTGTTAATGGGTCTCGGGAAAGGATG

GCCATTGTCAAAGATGGACATCGGAGTTCCCCTTCTCGCCATTGGATGCTACTCACAAGTCAACCCCATA

ACTCTCACAGCAGCTCTTTTCTTATTGGTAGCACATTATGCCATCATAGGGCCAGGACTCCAAGCAAAAG

CAACCAGAGAAGCTCAGAAAAGAGCAGCGGCGGGCATCATGAAAAACCCAACTGTCGATGGAATAACAGT

GATTGACCTAGATCCAATACCTTATGATCCAAAGTTTGAAAAGCAGTTGGGACAAGTAATGCTCCTAGTC

CTCTGCGTGACTCAAGTATTGATGATGAGGACTACATGGGCTCTGTGTGAGGCTTTAACCTTAGCTACCG
GGCCCATCTCCACATTGTGGGAAGGAAATCCAGGGAGGTTTTGGAACACTACCATTGCGGTGTCAATGGC

TAACATTTTTAGAGGGAGTTACTTGGCCGGAGCTGGACTTCTCTTTTCTATTATGAAGAACACAACCAAC

ACAAGAAGGGGAACTGGCAACATAGGAGAGACGCTTGGAGAGAAATGGAAAAGCCGATTGAACGCATTGG

GAAAAAGTGAATTCCAGATCTACAAGAAAAGTGGAATCCAGGAAGTGGATAGAACCTTAGCAAAAGAAGG

CATTAAAAGAGGAGAAACGGACCATCACGCTGTGTCGCGAGGCTCAGCAAAACTGAGATGGTTCGTTGAG

AGAAACATGGTCACACCAGAAGGGAAAGTAGTGGACCTCGGTTGTGGCAGAGGAGGCTGGTCATACTATT

GTGGAGGACTAAAGAATGTAAGAGAAGTCAAAGGCCTAACAAAAGGAGGACCAGGACACGAAGAACCCAT

CCCCATGTCAACATATGGGTGGAATCTAGTGCGTCTTCAAAGTGGAGTTGACGTTTTCTTCATCCCGCCA

GAAAAGTGTGACACATTATTGTGTGACATAGGGGAGTCATCACCAAATCCCACAGTGGAAGCAGGACGAA

CACTCAGAGTCCTTAACTTAGTAGAAAATTGGTTGAACAACAACACTCAATTTTGCATAAAGGTTCTCAA

CCCATATATGCCCTCAGTCATAGAAAAAATGGAAGCACTACAAAGGAAATATGGAGGAGCCTTAGTGAGG

AATCCACTCTCACGAAACTCCACACATGAGATGTACTGGGTATCCAATGCTTCCGGGAACATAGTGTCAT

CAGTGAACATGATTTCAAGGATGTTGATCAACAGATTTACAATGAGATACAAGAAAGCCACTTACGAGCC

GGATGTTGACCTCGGAAGCGGAACCCGTAACATCGGGATTGAAAGTGAGATACCAAACCTAGATATAATT

GGGAAAAGAATAGAAAAAATAAAGCAAGAGCATGAAACATCATGGCACTATGACCAAGACCACCCATACA

AAACGTGGGCATACCATGGTAGCTATGAAACAAAACAGACTGGATCAGCATCATCCATGGTCAACGGAGT

GGTCAGGCTGCTGACAAAACCTTGGGACGTCGTCCCCATGGTGACACAGATGGCAATGACAGACACGACT

CCATTTGGACAACAGCGCGTTTTTAAAGAGAAAGTGGACACGAGAACCCAAGAACCGAAAGAAGGCACGA

AGAAACTAATGAAAATAACAGCAGAGTGGCTTTGGAAAGAATTAGGGAAGAAAAAGACACCCAGGATGTG

CACCAGAGAAGAATTCACAAGAAAGGTGAGAAGCAATGCAGCCTTGGGGGCCATATTCACTGATGAGAAC

AAGTGGAAGTCGGCACGTGAGGCTGTTGAAGATAGTAGGTTTTGGGAGCTGGTTGACAAGGAAAGGAATC

TCCATCTTGAAGGAAAGTGTGAAACATGTGTGTACAACATGATGGGAAAAAGAGAGAAGAAGCTAGGGGA

ATTCGGCAAGGCAAAAGGCAGCAGAGCCATATGGTACATGTGGCTTGGAGCACGCTTCTTAGAGTTTGAA

GCCCTAGGATTCTTAAATGAAGATCACTGGTTCTCCAGAGAGAACTCCCTGAGTGGAGTGGAAGGAGAAG

GGCTGCACAAGCTAGGTTACATTCTAAGAGACGTGAGCAAGAAAGAGGGAGGAGCAATGTATGCCGATGA

CACCGCAGGATGGGATACAAGAATCACACTAGAAGACCTAAAAAATGAAGAAATGGTAACAAACCACATG

GAAGGAGAACACAAGAAACTAGCCGAGGCCATTTTCAAACTAACGTACCAAAACAAGGTGGTGCGTGTGC
AAAGACCAACACCAAGAGGCACAGTAATGGACATCATATCGAGAAGAGACCAAAGAGGTAGTGGACAAGT

TGGCACCTATGGACTCAATACTTTCACCAATATGGAAGCCCAACTAATCAGACAGATGGAGGGAGAAGGA

GTCTTTAAAAGCATTCAGCACCTAACAATCACAGAAGAAATCGCTGTGCAAAACTGGTTAGCAAGAGTGG

GGCGCGAAAGGTTATCAAGAATGGCCATCAGTGGAGATGATTGTGTTGTGAAACCTTTAGATGACAGGTT

CGCAAGCGCTTTAACAGCTCTAAATGACATGGGAAAGATTAGGAAAGACATACAACAATGGGAACCTTCA

AGAGGATGGAATGATTGGACACAAGTGCCCTTCTGTTCACACCATTTCCATGAGTTAATCATGAAAGACG

GTCGCGTACTCGTTGTTCCATGTAGAAACCAAGATGAACTGATTGGCAGAGCCCGAATCTCCCAAGGAGC

AGGGTGGTCTTTGCGGGAGACGGCCTGTTTGGGGAAGTCTTACGCCCAAATGTGGAGCTTGATGTACTTC

CACAGACGCGACCTCAGGCTGGCGGCAAATGCTATTTGCTCGGCAGTACCATCACATTGGGTTCCAACAA

GTCGAACAACCTGGTCCATACATGCTAAACATGAATGGATGACAACGGAAGACATGCTGACAGTCTGGAA

CAGGGTGTGGATTCAAGAAAACCCATGGATGGAAGACAAAACTCCAGTGGAATCATGGGAGGAAATCCCA

TACTTGGGGAAAAGAGAAGACCAATGGTGCGGCTCATTGATTGGGTTAACAAGCAGGGCCACCTGGGCAA

AGAACATCCAAGCAGCAATAAATCAAGTTAGATCCCTTATAGGCAATGAAGAATACACAGATTACATGCC

ATCCATGAAAAGATTCAGAAGAGAAGAGGAAGAAGCAGGAGTTCTGTGGTAGAAAGCAAAACTAACATGA

AACAAGGCTAGAAGTCAGGTCGGATTAAGCCATAGTACGGAAAAAACTATGCTACCTGTGAGCCCCGTCC

AAGGACGTTAAAAGAAGTCAGGCCATCATAAATGCCATAGCTTGAGTAAACTATGCAGCCTGTAGCTCCA

CCTGAGAAGGTGTAAAAAATCCGGGAGGCCACAAACCATGGAAGCTGTACGCATGGCGTAGTGGACTAGC

GGTTAGAGGAGACCCCTCCCTTACAAATCGCAGCAACAATGGGGGCCCAAGGCGAGATGAAGCTGTAGTC

TCGCTGGAAGGACTAGAGGTTAGAGGAGACCCCCCCGAAACAAAAAACAGCATATTGACGCTGGGAAAGA

CCAGAGATCCTGCTGTCTCCTCAGCATCATTCCAGGCACAGAACGCCAGAAAATGGAATGGTGCTGTTGA

ATCAACAGGTTCT.
Dengue Virus Type 3

>gi|37543955|gb|AY099336.1| Dengue virus type 3 isolate D3/H/IMTSSA-SRI/2000/1266, complete

genome

AGTTGTTAGTCTACGTGGACCGACAAGAACAGTTTCGACTCGGAAGCTTGCTTAACGTAGTGCTGACAGT

TTTTTATTAGAGAGCAGATCTCTGATGAACAACCAACGGAAGAAGACGGGAAAACCGTCTATCAATATGC

TGAAACGCGTGAGAAACCGTGTGTCAACTGGATCACAGTTGGCGAAGAGATTCTCAAAAGGACTGCTGAA

CGGCCAGGGACCAATGAAATTGGTTATGGCGTTCATAGCTTTCCTCAGATTTCTAGCCATTCCACCAACA

GCAGGAGTCTTGGCTAGATGGGGAACCTTCAAGAAGTCGGGGGCCATTAAGGTCCTGAAAGGCTTCAAGA

AGGAGATCTCAAACATGCTGAGCATAATCAACCAACGGAAAAAGACATCGCTCTGTCTCATGATGATATT

GCCAGCAGCACTTGCTTTCCACTTGACTTCACGAGATGGAGAGCCGCGCATGATTGTGGGGAAGAATGAA

AGAGGTAAATCCCTACTTTTTAAGACAGCCTCTGGAATCAACATGTGCACACTCATAGCCATGGATTTGG

GAGAGATGTGTGATGACACGGTCACTTACAAATGCCCCCACATTACCGAAGTGGAACCTGAAGACATTGA

CTGCTGGTGCAACCTTACATCAACATGGGTGACTTATGGAACGTGCAATCAAGCTGGAGAGCATAGACGC

GACAAGAGATCAGTGGCGTTAGCTCCCCATGTCGGCATGGGACTGGACACACGCACCCAAACCTGGATGT

CGGCTGAAGGAGCTTGGAGACAAGTCGAGAAGGTAGAGACATGGGCCCTTAGGCACCCAGGGTTCACCAT

ACTAGCCCTATTTCTCGCCCATTACATAGGCACTTCCCTGACCCAGAAGGTGGTTATTTTCATATTATTA

ATGCTGGTCACCCCATCCATGACAATGAGATGTGTGGGAGTAGGAAACAGAGATTTTGTGGAAGGGCTAT

CAGGAGCTACGTGGGTTGACGTGGTGCTCGAGCACGGGGGGTGTGTGACTACCATGGCTAAGAACAAGCC

CACGCTGGATATAGAGCTTCAGAAGACCGAGGCCACCCAACTGGCGACCCTAAGGAAGCTATGCATTGAG

GGGAAAATTACCAACATAACAACTGACTCAAGATGTCCTACCCAAGGGGAAGCGGTTTTGCCTGAGGAGC

AGGACCAGAACTACGTGTGTAAGCATACATACGTAGACAGAGGTTGGGGGAACGGTTGTGGTTTGTTTGG

CAAAGGAAGCTTGGTAACATGTGCGAAATTTCAATGCCTGGAACCAATAGAGGGAAAAGTGGTGCAATAT

GAGAACCTCAAATACACCGTCATCATTACAGTGCACACAGGAGACCAACACCAGGTGGGAAATGAAACGC

AAGGAGTCACGGCTGAGATAACACCTCAGGCATCAACCACTGAAGCCATCTTGCCTGAATATGGAACCCT

TGGGCTAGAATGCTCACCACGGACAGGTTTGGATTTCAATGAAATGATCTTACTAACAATGAAGAACAAA

GCATGGATGGTACATAGACAATGGTTCTTTGACCTACCTCTACCATGGGCATCAGGAGCTACAACAGAAA

CACCAACCTGGAACAGGAAGGAGCTTCTTGTGACATTCAAAAACGCACATGCGAAAAAACAAGAAGTAGT
TGTCCTTGGATCGCAAGAGGGAGCAATGCATACCGCACTGACAGGAGCTACAGAAATCCAAAACTCAGGA

GGCACAAGCATTTTCGCGGGGCACTTAAAATGTAGACTTAAGATGGACAAATTGGAACTCAAGGGGATGA

GCTATGCAATGTGCACGAATACCTTTGTGTTGAAGAAAGAAGTCTCAGAAACGCAGCACGGGACAATACT

CATTAAGGTTGAGTACAAAGGGGAAGATGCACCTTGCAAGATTCCCTTTTCCACAGAGGATGGACAAGGG

AAAGCTCATAATGGCAGACTGATCACAGCCAACCCTGTGGTGACTAAGAAGGAGGAGCCTGTCAATATTG

AGGCTGAACCTCCTTTTGGGGAAAGCAATATAGTAATTGGAATTGGAGACAACGCCTTGAAAATCAACTG

GTACAAGAAGGGGAGCTCGATTGGGAAGATGTTCGAGGCCACTGAAAGGGGTGCAAGGCGCATGGCCATC

TTGGGAGACACAGCTTGGGACTTTGGATCAGTGGGTGGTGTTCTGAACTCATTAGGCAAAATGGTGCACC

AAATATTTGGAAGTGCTTATACAGCCCTGTTCAGTGGAGTCTCTTGGGTGATGAAAATTGGAATAGGTGT

CCTCTTGACTTGGATAGGGTTGAATTCAAAAAACACATCCATGTCATTTTCATGCATTGCGATAGGAATC

ATTACACTCTATCTGGGAGCTGTGGTACAAGCTGACATGGGGTGTGTCATAAACTGGAAGGGCAAAGAAC

TCAAATGTGGAAGCGGAATTTTCGTCACCAATGAGGTCCATACCTGGACAGAGCAATACAAATTCCAAGC

AGACTCCCCAAAAAGATTGGCAACAGCCATTGCAGGCGCCTGGGAGAATGGAGTGTGTGGAATTAGGTCA

ACAACCAGAATGGAGAATCTCTTGTGGAAGCAAATAGCCAATGAACTGAACTACATATTATGGGAAAACA

ATATCAAATTAACGGTAGTTGTGGGCGATACACTTGGGGTCTTAGAGCAAGGGAAAAGAACACTAACACC

ACAACCCATGGAGCTAAAATACTCATGGAAAACGTGGGGAAAGGCAAAAATAGTGACAGCTGAAACACAA

AATTCCTCTTTCATAATAGACGGGCCAAACACACCGGAGTGTCCAAGTGCCTCAAGAGCATGGAATGTGT

GGGAGGTGGAAGATTACGGGTTCGGAGTCTTCACAACCAACATATGGCTGAAACTCCGAGAGGTCTACAC

CCAACTATGTGACCATAGGCTAATGTCGGCAGCTGTCAAGGATGAGAGGGCCGTGCATGCCGACATGGGC

TACTGGATAGAAAGCCAAAAGAATGGAAGTTGGAAGCTAGAAAAAGCATCCCTCATAGAGGTAAAAACCT

GCACATGGCCAAAATCACACACTCTCTGGACTAATGGTGTGCTAGAGAGTGACATGATCATCCCAAAGAG

TCTAGCTGGTCCTATCTCACAACACAACTACAGGCCCGGGTACCACACCCAAACGGCAGGACCCTGGCAC

TTAGGAAAATTGGAGCTGGACTTCAACTACTGTGAAGGAACAACAGTTGTCATCACAGAAAGCTGTGGGA

CAAGAGGCCCATCATTGAGAACAACAACAGTGTCAGGGAAGTTGATACACGAATGGTGTTGCCGCTCGTG

CACACTTCCCCCCCTGCGATACATGGGAGAAGACGGCTGCTGGTATGGCATGGAAATCAGACCCATCAGT

GAGAAAGAAGAGAACATGGTAAAGTCTTTAGTCTCAGCGGGAAGTGGAAAGGTGGACAACTTCACAATGG

GTGTCTTGTGTTTGGCAATCCTCTTTGAAGAGGTGTTGAGAGGAAAATTTGGGAAGAAACACATGATTGC
AGGGGTTTTCTTTACGTTTGTGCTCCTTCTCTCAGGGCAAATAACATGGAGAGACATGGCGCACACACTA

ATAATGATCGGGTCCAACGCCTCTGACAGGATGGGAATGGGCGTCACCTACCTAGCTCTAATTGCAACAT

TTAAAATCCAGCCATTCTTGGCTTTGGGATTTTTCCTAAGAAAGCTGACATCTAGAGAAAATTTATTGTT

AGGAGTTGGGTTGGCCATGGCAACAACGTTACAACTGCCAGAGGACATTGAACAAATGGCAAATGGAGTC

GCTCTGGGGCTCATGGCTCTTAAACTGATAACACAATTTGAAACATACCAATTGTGGACGGCATTAGTCT

CCTTAACGTGTTCAAACACAATTTTTACGTTGACTGTTGCCTGGAGAACAGCCACTCTGATTTTGGCCGG

AGTTTCGCTTTTACCAGTGTGCCAGTCTTCAAGCATGAGGAAAACAGATTGGCTCCCAATGACAGTGGCA

GCTATGGGAGTTCCACCCCTTCCACTTTTTATTTTTAGCTTGAAAGACACACTCAAAAGGAGAAGCTGGC

CACTGAATGAAGGGGTGATGGCTGTTGGGCTTGTGAGCATTCTGGCCAGTTCTCTCCTTAGAAATGATGT

GCCCATGGCTGGACCATTAGTGGCCGGGGGCTTGCTGATAGCGTGCTACGTCATAACTGGCACGTCAGCG

GACCTCACTGTAGAAAAAGCCCCAGATGTAACATGGGAGGAAGAGGCTGAGCAGACAGGAGTGTCCCACA

ACTTAATGATCACAGTTGATGATGATGGAACAATGAGAATAAAAGATGATGAGACTGAGAACATCCTAAC

AGTGCTTTTAAAAACAGCATTACTAATAGTATCAGGCATTTTTCCATACTCCATACCCGCAACATTGTTG

GTCTGGCACACTTGGCAAAAACAAACCCAAAGATCCGGCGTTTTATGGGACGTACCCAGCCCCCCAGAGA

CACAGAAAGCAGAACTGGAAGAAGGGGTTTATAGGATCAAACAGCAAGGAATTTTTGGGAAAACCCAAGT

AGGGGTTGGAGTACAGAAAGAAGGAGTCTTCCACACCATGTGGCACGTCACAAGAGGGGCAGTGTTGACA

CATAATGGGAAAAGACTGGAACCAAACTGGGCTAGTGTGAAAAAAGATCTGATTTCATATGGAGGAGGAT

GGAGACTGAGCGCACAATGGCAAAAGGGGGAGGAGGTGCAGGTTATTGCCGTAGAGCCAGGGAAGAACCC

AAAGAACTTTCAAACCACGCCAGGCACTTTCCAGACTACTACAGGGGAAATAGGAGCAATTGCACTGGAT

TTCAAGCCTGGAACTTCAGGATCTCCTATCATAAATAGAGAGGGAAAGGTAGTGGGACTGTATGGCAATG

GAGTGGTTACAAAGAATGGTGGCTATGTCAGCGGAATAGCGCAAACAAATGCAGAACCAGATGGACCGAC

ACCAGAGTTGGAAGAAGAGATGTTCAAAAAGCGAAACCTGACCATAATGGATCTTCATCCTGGGTCAGGA

AAGACACGGAAATACCTTCCAGCTATTGTCAGAGAGGCAATCAAGAGACGTTTAAGAACCTTAATTTTGG

CACCGACAAGGGTGGTTGCAGCTGAGATGGAAGAAGCATTGAAAGGGCTCCCAATAAGGTACCAAACAAC

AGCAACAAAATCTGAACACACAGGAAGAGAGATTGTTGATCTAATGTGCCACGCAACGTTCACAATGCGT

TTGCTGTCACCAGTTAGGGTTCCAAATTACAACTTGATAATAATGGATGAGGCCCATTTCACAGACCCAG

CCAGTATAGCGGCTAGAGGGTACATATCAACTCGTGTTGGAATGGGAGAGGCAGCCGCAATCTTCATGAC
AGCAACACCCCCTGGAACAGCTGATGCCTTTCCTCAGAGCAACGCTCCAATTCAAGATGAAGAAAGGGAC

ATACCAGAACGCTCATGGAATTCAGGCAATGAATGGATTACCGACTTCGCTGGGAAAACGGTGTGGTTTG

TCCCTAGCATTAAAGCCGGAAATGACATAGCAAACTGCTTGCGAAAAAACGGGAAAAAAGTCATTCAACT

TAGTAGGAAGACTTTTGACACAGAATATCAGAAGACTAAACTGAATGATTGGGACTTTGTGGTGACAACT

GACATTTCAGAAATGGGGGCCAATTTCAAAGCAGATAGAGTGATCGACCCAAGAAGATGTCTCAAACCAG

TGATCTTGACAGATGGACCAGAGCGGGTGATCCTGGCCGGACCAATGCCAGTCACCGCGGCGAGTGCTGC

GCAAAGGAGAGGGAGAGTTGGCAGGAACCCACAAAAAGAGAATGACCAGTACATATTCACGGGCCAGCCT

CTCAACAATGATGAAGACCATGCTCACTGGACAGAAGCAAAAATGCTGCTGGACAACATCAACACACCAG

AAGGGATTATACCAGCTCTCTTTGAACCAGAAAGGGAGAAGTCAGCCGCCATAGACGGTGAGTATCGCCT

GAAGGGTGAGTCCAGGAAGACTTTCGTGGAACTCATGAGGAGGGGTGACCTTCCAGTTTGGTTAGCCCAT

AAAGTAGCATCAGAAGGAATCAAATACACAGATAGAAAATGGTGCTTTGATGGGCAACGCAATAATCAAA

TTTTAGAGGAGAACATGGATGTGGAAATTTGGACAAAGGAAGGAGAAAAGAAAAAATTGAGACCTAGGTG

GCTTGATGCCCGCACTTATTCAGATCCATTGGCACTCAAGGAATTCAAGGACTTTGCGGCTGGCAGAAAG

TCAATCGCCCTTGATCTTGTGACAGAAATAGGAAGAGTGCCTTCACATCTAGCCCACAGAACAAGAAACG

CTCTGGACAATCTGGTGATGCTGCATACGTCAGAAGATGGCGGTAGGGCTTACAGGCATGCGGTGGAGGA

ACTACCAGAAACAATGGAAACACTCCTACTCTTGGGACTAATGATCTTGTTGACAGGTGGAGCAATGCTT

TTCTTGATATCAGGTAAAGGGATTGGAAAGACTTCAATAGGACTCATTTGTGTAATCGCTTCCAGCGGCA

TGTTGTGGATGGCCGAAGTTCCACTCCAATGGATCGCGTCGGCTATAGTCCTGGAGTTTTTTATGATGGT

GTTGCTCATACCAGAACCAGAAAAGCAGAGAACCCCCCAAGACAACCAACTCGCATATGTCGTGATAGGC

ATACTTACATTGGCTGCAACAATAGCAGCCAATGAAATGGGACTGCTGGAAACCACAAAGAGAGACTTAG

GAATGTCTAAGGAGCCAGGTGTTGTTTCTCCAACCAGCTATTTGGATGTGGACTTGCACCCAGCATCAGC

CTGGACATTGTACGCCGTGGCCACTACAGTAATAACACCAATGTTAAGACATACCATAGAGAATTCTACA

GCAAATGTGTCCCTGGCAGCTATAGCCAACCAGGCAGTGGTCCTGATGGGTTTGGACAAAGGATGGCCAA

TATCAAAAATGGACTTAGGCGTGCCACTACTGGCACTGGGTTGCTATTCACAAGTGAACCCACTGACTCT

AACTGCGGCAGTACTTTTGCTAATCACACATTATGCTATCATAGGTCCAGGATTGCAAGCAAAAGCCACC

CGTGAAGCTCAGAAAAGGACAGCTGCTGGAATAATGAAGAATCCAACAGTGGATGGGATAATGACAATAG

ACCTAGATTCTGTAATATTTGATTCAAAATTTGAAAAACAACTGGGACAGGTTATGCTCCTGGTTTTGTG
CGCAGTCCAACTCTTGCTAATGAGAACATCATGGGCCTTGTGTGAAGCTTTAACTCTAGCTACAGGACCA

ATAACAACACTCTGGGAAGGATCACCTGGTAAGTTCTGGAACACCACGATAGCTGTTTCCATGGCGAACA

TTTTTAGAGGGAGCTATTTAGCAGGAGCTGGGCTTGCTTTTTCTATTATGAAATCAGTTGGAACAGGAAA

AAGAGGAACAGGCTCACAAGGTGAAACTTTAGGAGAAAAATGGAAAAAGAAATTAAATCAATTATCCCGG

AAAGAGTTTGACCTTTACAAGAAATCTGGAATCACTGAAGTGGATAGAACAGAAGCCAAAGAAGGGTTGA

AAAGAGGAGAGACAACACATCATGCCGTGTCCCGAGGTAGCGCAAAACTTCAATGGTTTGTGGAAAGAAA

CATGGTCGTTCCCGAAGGAAGAGTCATAGACTTGGGCTGTGGAAGAGGAGGCTGGTCATATTACTGTGCA

GGACTGAAAAAAGTCACAGAAGTGCGAGGATACACAAAAGGCGGTCCAGGACACGAAGAACCAGTACCTA

TGTCTACATATGGATGGAACATAGTTAAGTTAATGAGCGGAAAGGATGTGTTCTATCTCCCACCTGAAAA

GTGTGATACCCTGTTGTGTGACATTGGAGAATCTTCACCAAGCCCAACAGTGGAAGAGAGCAGAACTATA

AGAGTTTTGAAGATGGTTGAACCATGGCTAAAAAACAACCAGTTTTGCATTAAAGTTTTGAACCCTTACA

TGCCAACTGTGATTGAGCACCTAGAAAGACTACAAAGGAAACATGGAGGAATGCTTGTGAGAAATCCACT

TTCACGAAACTCCACGCACGAAATGTACTGGATATCTAATGGCACAGGTAACATTGTCTCTTCAGTCAAC

ATGGTGTCTAGATTGCTACTGAACAGGTTCACGATGACACACAGGAGACCCACCATAGAGAAAGATGTGG

ATTTAGGAGCAGGAACTCGACATGTTAATGCGGAACCAGAAACACCCAACATGGATGTCATTGGGGAAAG

AATAAAAAGGATCAAGGAGGAGCATAATTCAACATGGCACTATGATGACGAAAACCCCTACAAAACGTGG

GCTTACCATGGATCCTATGAAGTCAAAGCCACAGGCTCAGCCTCCTCCATGATAAATGGAGTCGTGAAAC

TCCTCACCAAACCATGGGATGTGGTGCCCATGGTGACACAGATGGCAATGACAGACACAACTCCATTTGG

CCAGCAGAGAGTCTTTAAAGAGAAAGTGGACACCAGGACGCCCAGGCCCATGCCAGGGACAAGAAAGGCT

ATGGAGATCACAGCGGAGTGGCTCTGGAGAACCCTGGGAAGGAACAAAAGACCCAGATTATGCACAAGGG

AAGAGTTTACAAAAAAGGTCAGAACTAACGCAGCCATGGGCGCCGTTTTCACAGAGGAGAACCAATGGGA

CAGTGCGAAAGCTGCTGTTGAGGATGAAGAATTTTGGAAACTTGTGGACAGAGAACGTGAACTCCACAAA

TTGGGCAAATGTGGAAGCTGCGTTTATAACATGATGGGCAAGAGAGAGAAAAAACTTGGAGAGTTTGGCA

AAGCAAAAGGCAGTAGAGCTATATGGTACATGTGGTTGGGAGCCAGGTACCTTGAGTTCGAAGCCCTTGG

ATTCTTAAATGAAGACCACTGGTTCTCGCGTGAAAACTCTTACAGTGGAGTAGAAGGAGAAGGACTGCAC

AAGCTAGGCTACATATTAAGGGACATTTCCAAGATACCCGGAGGAGCCATGTATGCTGATGACACAGCTG

GTTGGGACACAAGAATAACAGAAGATGACCTGCACAATGAGGAAAAGATCATACAGCAAATGGACCCTGA
ACACAGGCAGTTAGCGAACGCTATATTCAAGCTCACATACCAAAACAAAGTGGTCAAAGTTCAACGACCG

ACTCCAACGGGCACGGTAATGGATATTATATCTAGGAAAGACCAAAGGGGCAGTGGACAACTGGGAACTT

ATGGCCTGAATACATTCACCAACATGGAAGCCCAGTTAGTCAGACAAATGGAAGGAGAAGGTGTGCTGAC

AAAGGCAGACCTCGAGAACCCTCATCTGCTAGAGAAGAAAATCACACAATGGTTGGAAACCAAAGGAGTG

GAGAGGTTAAAAAGAATGGCCATTAGCGGGGATGATTGCGTGGTGAAACCAATCGATGACAGGTTCGCTA

ATGCCCTGCTTGCTTTGAACGATATGGGAAAGGTTCGGAAAGACATACCTCAATGGCAGCCATCAAAGGG

ATGGCATGATTGGCAACAGGTTCCTTTCTGCTCCCACCACTTTCATGAATTGATCATGAAAGATGGAAGA

AAGTTGGTGGTTCCCTGCAGACCCCAGGACGAACTAATAGGAAGAGCAAGAATCTCTCAAGGAGCGGGAT

GGAGCCTTAGAGAAACTGCATGTCTGGGGAAAGCCTACGCCCAAATGTGGAGTCTCATGTATTTTCACAG

AAGAGATCTCAGATTAGCATCCAACGCCATATGTTCAGCAGTACCAGTCCACTGGGTTCCCACAAGTAGA

ACGACATGGTCTATTCATGCTCACCATCAGTGGATGACTACAGAAGACATGCTTACTGTTTGGAACAGGG

TGTGGATAGAGGAAAATCCATGGATGGAAGACAAAACTCCAGTTACAACTTGGGAAAATGTTCCATATCT

AGGAAAGAGAGAAGACCAATGGTGTGGATCACTTATTGGTCTCACTTCCAGAGCAACCTGGGCCCAGAAC

ATACCCACAGCAATTCAACAGGTGAGAAGCCTTATAGGCAATGAAGAGTTCCTGGACTACATGCCTTCAA

TGAAGAGATTCAGGAAGGAAGAGGAGTCGGAGGGAGCCATTTGGTAAACGTAGGAAGTGGAAAAGAGGCT

AACTGTCAGGCCACCTTAAGCCACAGTACGGAAGAAGCTGTGCTGCCTGTGAGCCCCGTCCAAGGACGTT

AAAAGAAGAAGTCAGGCCCCAAAGCCACGGTTTGAGCAAACCGTGCTGCCTGTAGCTCCGTCGTGGGGAC

GTAAAACCTGGGAGGCTGCAAACTGTGGAAGCTGTACGCACGGTGTAGCAGACTAGCGGTTAGAGGAGAC

CCCTCCCATGACACAACGCAGCAGCGGGGCCCGAGCACTGAGGGAAGCTGTACCTCCTTGCAAAGGACTA

GAGGTTAGAGGAGACCCCCCGCAAATAAAAACAGCATATTGACGCTGGGAGAGACCAGAGATCCTGCTGT

CTCCTCAGCATCATTCCAGGCACAGAACGCCAGAAAATGGAATGGTGCTGTTGAATCAACAGGTTCT
Dengue Virus Type 4

>gi|12018169|gb|AF326825.1| Dengue virus type 4 recombinant clone rDEN4, complete sequence

AGTTGTTAGTCTGTGTGGACCGACAAGGACAGTTCCAAATCGGAAGCTTGCTTAACACAGTTCTAACAGT

TTGTTTGAATAGAGAGCAGATCTCTGGAAAAATGAACCAACGAAAAAAGGTGGTTAGACCACCTTTCAAT

ATGCTGAAACGCGAGAGAAACCGCGTATCAACCCCTCAAGGGTTGGTGAAGAGATTCTCAACCGGACTTT

TTTCTGGGAAAGGACCCTTACGGATGGTGCTAGCATTCATCACGTTTTTGCGAGTCCTTTCCATCCCACC

AACAGCAGGGATTCTGAAGAGATGGGGACAGTTGAAGAAAAATAAGGCCATCAAGATACTGATTGGATTC

AGGAAGGAGATAGGCCGCATGCTGAACATCTTGAACGGGAGAAAAAGGTCAACGATAACATTGCTGTGCT

TGATTCCCACCGTAATGGCGTTTTCCCTCAGCACAAGAGATGGCGAACCCCTCATGATAGTGGCAAAACA

TGAAAGGGGGAGACCTCTCTTGTTTAAGACAACAGAGGGGATCAACAAATGCACTCTCATTGCCATGGAC

TTGGGTGAAATGTGTGAGGACACTGTCACGTATAAATGCCCCCTACTGGTCAATACCGAACCTGAAGACA

TTGATTGCTGGTGCAACCTCACGTCTACCTGGGTCATGTATGGGACATGCACCCAGAGCGGAGAACGGAG

ACGAGAGAAGCGCTCAGTAGCTTTAACACCACATTCAGGAATGGGATTGGAAACAAGAGCTGAGACATGG

ATGTCATCGGAAGGGGCTTGGAAGCATGCTCAGAGAGTAGAGAGCTGGATACTCAGAAACCCAGGATTCG

CGCTCTTGGCAGGATTTATGGCTTATATGATTGGGCAAACAGGAATCCAGCGAACTGTCTTCTTTGTCCT

AATGATGCTGGTCGCCCCATCCTACGGAATGCGATGCGTAGGAGTAGGAAACAGAGACTTTGTGGAAGGA

GTCTCAGGTGGAGCATGGGTCGACCTGGTGCTAGAACATGGAGGATGCGTCACAACCATGGCCCAGGGAA

AACCAACCTTGGATTTTGAACTGACTAAGACAACAGCCAAGGAAGTGGCTCTGTTAAGAACCTATTGCAT

TGAAGCCTCAATATCAAACATAACTACGGCAACAAGATGTCCAACGCAAGGAGAGCCTTATCTGAAAGAG

GAACAGGACCAACAGTACATTTGCCGGAGAGATGTGGTAGACAGAGGGTGGGGCAATGGCTGTGGCTTGT

TTGGAAAAGGAGGAGTTGTGACATGTGCGAAGTTTTCATGTTCGGGGAAGATAACAGGCAATTTGGTCCA

AATTGAGAACCTTGAATACACAGTGGTTGTAACAGTCCACAATGGAGACACCCATGCAGTAGGAAATGAC

ACATCCAATCATGGAGTTACAGCCATGATAACTCCCAGGTCACCATCGGTGGAAGTCAAATTGCCGGACT

ATGGAGAACTAACACTCGATTGTGAACCCAGGTCTGGAATTGACTTTAATGAGATGATTCTGATGAAAAT

GAAAAAGAAAACATGGCTCGTGCATAAGCAATGGTTTTTGGATCTGCCTCTTCCATGGACAGCAGGAGCA

GACACATCAGAGGTTCACTGGAATTACAAAGAGAGAATGGTGACATTTAAGGTTCCTCATGCCAAGAGAC
AGGATGTGACAGTGCTGGGATCTCAGGAAGGAGCCATGCATTCTGCCCTCGCTGGAGCCACAGAAGTGGA

CTCCGGTGATGGAAATCACATGTTTGCAGGACATCTTAAGTGCAAAGTCCGTATGGAGAAATTGAGAATC

AAGGGAATGTCATACACGATGTGTTCAGGAAAGTTTTCAATTGACAAAGAGATGGCAGAAACACAGCATG

GGACAACAGTGGTGAAAGTCAAGTATGAAGGTGCTGGAGCTCCGTGTAAAGTCCCCATAGAGATAAGAGA

TGTAAACAAGGAAAAAGTGGTTGGGCGTATCATCTCATCCACCCCTTTGGCTGAGAATACCAACAGTGTA

ACCAACATAGAATTAGAACCCCCCTTTGGGGACAGCTACATAGTGATAGGTGTTGGAAACAGCGCATTAA

CACTCCATTGGTTCAGGAAAGGGAGTTCCATTGGCAAGATGTTTGAGTCCACATACAGAGGTGCAAAACG

AATGGCCATTCTAGGTGAAACAGCTTGGGATTTTGGTTCCGTTGGTGGACTGTTCACATCATTGGGAAAG

GCTGTGCACCAGGTTTTTGGAAGTGTGTATACAACCATGTTTGGAGGAGTCTCATGGATGATTAGAATCC

TAATTGGGTTCTTAGTGTTGTGGATTGGCACGAACTCGAGGAACACTTCAATGGCTATGACGTGCATAGC

TGTTGGAGGAATCACTCTGTTTCTGGGCTTCACAGTTCAAGCAGACATGGGTTGTGTGGCGTCATGGAGT

GGGAAAGAATTGAAGTGTGGAAGCGGAATTTTTGTGGTTGACAACGTGCACACTTGGACAGAACAGTACA

AATTTCAACCAGAGTCCCCAGCGAGACTAGCGTCTGCAATATTAAATGCCCACAAAGATGGGGTCTGTGG

AATTAGATCAACCACGAGGCTGGAAAATGTCATGTGGAAGCAAATAACCAACGAGCTAAACTATGTTCTC

TGGGAAGGAGGACATGACCTCACTGTAGTGGCTGGGGATGTGAAGGGGGTGTTGACCAAAGGCAAGAGAG

CACTCACACCCCCAGTGAGTGATCTGAAATATTCATGGAAGACATGGGGAAAAGCAAAAATCTTCACCCC

AGAAGCAAGAAATAGCACATTTTTAATAGACGGACCAGACACCTCTGAATGCCCCAATGAACGAAGAGCA

TGGAACTCTCTTGAGGTGGAAGACTATGGATTTGGCATGTTCACGACCAACATATGGATGAAATTCCGAG

AAGGAAGTTCAGAAGTGTGTGACCACAGGTTAATGTCAGCTGCAATTAAAGATCAGAAAGCTGTGCATGC

TGACATGGGTTATTGGATAGAGAGCTCAAAAAACCAGACCTGGCAGATAGAGAAAGCATCTCTTATTGAA

GTGAAAACATGTCTGTGGCCCAAGACCCACACACTGTGGAGCAATGGAGTGCTGGAAAGCCAGATGCTCA

TTCCAAAATCATATGCGGGCCCTTTTTCACAGCACAATTACCGCCAGGGCTATGCCACGCAAACCGTGGG

CCCATGGCACTTAGGCAAATTAGAGATAGACTTTGGAGAATGCCCCGGAACAACAGTCACAATTCAGGAG

GATTGTGACCATAGAGGCCCATCTTTGAGGACCACCACTGCATCTGGAAAACTAGTCACGCAATGGTGCT

GCCGCTCCTGCACGATGCCTCCCTTAAGGTTCTTGGGAGAAGATGGGTGCTGGTATGGGATGGAGATTAG

GCCCTTGAGTGAAAAAGAAGAGAACATGGTCAAATCACAGGTGACGGCCGGACAGGGCACATCAGAAACT

TTTTCTATGGGTCTGTTGTGCCTGACCTTGTTTGTGGAAGAATGCTTGAGGAGAAGAGTCACTAGGAAAC
ACATGATATTAGTTGTGGTGATCACTCTTTGTGCTATCATCCTGGGAGGCCTCACATGGATGGACTTACT

ACGAGCCCTCATCATGTTGGGGGACACTATGTCTGGTAGAATAGGAGGACAGATCCACCTAGCCATCATG

GCAGTGTTCAAGATGTCACCAGGATACGTGCTGGGTGTGTTTTTAAGGAAACTCACTTCAAGAGAGACAG

CACTAATGGTAATAGGAATGGCCATGACAACGGTGCTTTCAATTCCACATGACCTTATGGAACTCATTGA

TGGAATATCACTGGGACTAATTTTGCTAAAAATAGTAACACAGTTTGACAACACCCAAGTGGGAACCTTA

GCTCTTTCCTTGACTTTCATAAGATCAACAATGCCATTGGTCATGGCTTGGAGGACCATTATGGCTGTGT

TGTTTGTGGTCACACTCATTCCTTTGTGCAGGACAAGCTGTCTTCAAAAACAGTCTCATTGGGTAGAAAT

AACAGCACTCATCCTAGGAGCCCAAGCTCTGCCAGTGTACCTAATGACTCTTATGAAAGGAGCCTCAAGA

AGATCTTGGCCTCTTAACGAGGGCATAATGGCTGTGGGTTTGGTTAGTCTCTTAGGAAGCGCTCTTTTAA

AGAATGATGTCCCTTTAGCTGGCCCAATGGTGGCAGGAGGCTTACTTCTGGCGGCTTACGTGATGAGTGG

TAGCTCAGCAGATCTGTCACTAGAGAAGGCCGCCAACGTGCAGTGGGATGAAATGGCAGACATAACAGGC

TCAAGCCCAATCGTAGAAGTGAAGCAGGATGAAGATGGCTCTTTCTCCATACGGGACGTCGAGGAAACCA

ATATGATAACCCTTTTGGTGAAACTGGCACTGATAACAGTGTCAGGTCTCTACCCCTTGGCAATTCCAGT

CACAATGACCTTATGGTACATGTGGCAAGTGAAAACACAAAGATCAGGAGCCCTGTGGGACGTCCCCTCA

CCCGCTGCCACTAAAAAAGCCGCACTGTCTGAAGGAGTGTACAGGATCATGCAAAGAGGGTTATTCGGGA

AAACTCAGGTTGGAGTAGGGATACACATGGAAGGTGTATTTCACACAATGTGGCATGTAACAAGAGGATC

AGTGATCTGCCACGAGACTGGGAGATTGGAGCCATCTTGGGCTGACGTCAGGAATGACATGATATCATAC

GGTGGGGGATGGAGGCTTGGAGACAAATGGGACAAAGAAGAAGACGTTCAGGTCCTCGCCATAGAACCAG

GAAAAAATCCTAAACATGTCCAAACGAAACCTGGCCTTTTCAAGACCCTAACTGGAGAAATTGGAGCAGT

AACATTAGATTTCAAACCCGGAACGTCTGGTTCTCCCATCATCAACAGGAAAGGAAAAGTCATCGGACTC

TATGGAAATGGAGTAGTTACCAAATCAGGTGATTACGTCAGTGCCATAACGCAAGCCGAAAGAATTGGAG

AGCCAGATTATGAAGTGGATGAGGACATTTTTCGAAAGAAAAGATTAACTATAATGGACTTACACCCCGG

AGCTGGAAAGACAAAAAGAATTCTTCCATCAATAGTGAGAGAAGCCTTAAAAAGGAGGCTACGAACTTTG

ATTTTAGCTCCCACGAGAGTGGTGGCGGCCGAGATGGAAGAGGCCCTACGTGGACTGCCAATCCGTTATC

AGACCCCAGCTGTGAAATCAGAACACACAGGAAGAGAGATTGTAGACCTCATGTGTCATGCAACCTTCAC

AACAAGACTTTTGTCATCAACCAGGGTTCCAAATTACAACCTTATAGTGATGGATGAAGCACATTTCACC

GATCCTTCTAGTGTCGCGGCTAGAGGATACATCTCGACCAGGGTGGAAATGGGAGAGGCAGCAGCCATCT
TCATGACCGCAACCCCTCCCGGAGCGACAGATCCCTTTCCCCAGAGCAACAGCCCAATAGAAGACATCGA

GAGGGAAATTCCGGAAAGGTCATGGAACACAGGGTTCGACTGGATAACAGACTACCAAGGGAAAACTGTG

TGGTTTGTTCCCAGCATAAAAGCTGGAAATGACATTGCAAATTGTTTGAGAAAGTCGGGAAAGAAAGTTA

TCCAGTTGAGTAGGAAAACCTTTGATACAGAGTATCCAAAAACGAAACTCACGGACTGGGACTTTGTGGT

CACTACAGACATATCTGAAATGGGGGCCAATTTTAGAGCCGGGAGAGTGATAGACCCTAGAAGATGCCTC

AAGCCAGTTATCCTACCAGATGGGCCAGAGAGAGTCATTTTAGCAGGTCCTATTCCAGTGACTCCAGCAA

GCGCTGCTCAGAGAAGAGGGCGAATAGGAAGGAACCCAGCACAAGAAGACGACCAATACGTTTTCTCCGG

AGACCCACTAAAAAATGATGAAGATCATGCCCACTGGACAGAAGCAAAGATGCTGCTTGACAATATCTAC

ACCCCAGAAGGGATCATTCCAACATTGTTTGGTCCGGAAAGGGAAAAAACCCAAGCCATTGATGGAGAGT

TTCGCCTCAGAGGGGAACAAAGGAAGACTTTTGTGGAATTAATGAGGAGAGGAGACCTTCCGGTGTGGCT

GAGCTATAAGGTAGCTTCTGCTGGCATTTCTTACGAAGATCGGGAATGGTGCTTCACAGGGGAAAGAAAT

AACCAAATTTTAGAAGAAAACATGGAGGTTGAAATTTGGACTAGAGAGGGAGAAAAGAAAAAGCTAAGGC

CAAGATGGTTAGATGCACGTGTATACGCTGACCCCATGGCTTTGAAGGATTTCAAGGAGTTTGCCAGTGG

AAGGAAGAGTATAACTCTCGACATCCTAACAGAGATTGCCAGTTTGCCAACTTACCTTTCCTCTAGGGCC

AAGCTCGCCCTTGATAACATAGTCATGCTCCACACAACAGAAAGAGGAGGGAGGGCCTATCAACACGCCC

TGAACGAACTTCCGGAGTCACTGGAAACACTCATGCTTGTAGCTTTACTAGGTGCTATGACAGCAGGCAT

CTTCCTGTTTTTCATGCAAGGGAAAGGAATAGGGAAATTGTCAATGGGTTTGATAACCATTGCGGTGGCT

AGTGGCTTGCTCTGGGTAGCAGAAATTCAACCCCAGTGGATAGCGGCCTCAATCATACTAGAGTTTTTTC

TCATGGTACTGTTGATACCGGAACCAGAAAAACAAAGGACCCCACAAGACAATCAATTGATCTACGTCAT

ATTGACCATTCTCACCATCATTGGTCTAATAGCAGCCAACGAGATGGGGCTGATTGAAAAAACAAAAACG

GATTTTGGGTTTTACCAGGTAAAAACAGAAACCACCATCCTCGATGTGGACTTGAGACCAGCTTCAGCAT

GGACGCTCTATGCAGTAGCCACCACAATTCTGACTCCCATGCTGAGACACACCATAGAAAACACGTCGGC

CAACCTATCTCTAGCAGCCATTGCCAACCAGGCAGCCGTCCTAATGGGGCTTGGAAAAGGATGGCCGCTC

CACAGAATGGACCTCGGTGTGCCGCTGTTAGCAATGGGATGCTATTCTCAAGTGAACCCAACAACCTTGA

CAGCATCCTTAGTCATGCTTTTAGTCCATTATGCAATAATAGGCCCAGGATTGCAGGCAAAAGCCACAAG

AGAGGCCCAGAAAAGGACAGCTGCTGGGATCATGAAAAATCCCACAGTGGACGGGATAACAGTAATAGAT

CTAGAACCAATATCCTATGACCCAAAATTTGAAAAGCAATTAGGGCAGGTCATGCTACTAGTCTTGTGTG
CTGGACAACTACTCTTGATGAGAACAACATGGGCTTTCTGTGAAGTCTTGACTTTGGCCACAGGACCAAT

CTTGACCTTGTGGGAGGGCAACCCGGGAAGGTTTTGGAACACGACCATAGCCGTATCCACCGCCAACATT

TTCAGGGGAAGTTACTTGGCGGGAGCTGGACTGGCTTTTTCACTCATAAAGAATGCACAAACCCCTAGGA

GGGGAACTGGGACCACAGGAGAGACACTGGGAGAGAAGTGGAAGAGACAGCTAAACTCATTAGACAGAAA

AGAGTTTGAAGAGTATAAAAGAAGTGGAATACTAGAAGTGGACAGGACTGAAGCCAAGTCTGCCCTGAAA

GATGGGTCTAAAATCAAGCATGCAGTATCAAGAGGGTCCAGTAAGATCAGATGGATTGTTGAGAGAGGGA

TGGTAAAGCCAAAAGGGAAAGTTGTAGATCTTGGCTGTGGGAGAGGAGGATGGTCTTATTACATGGCGAC

ACTCAAGAACGTGACTGAAGTGAAAGGGTATACAAAAGGAGGTCCAGGACATGAAGAACCGATTCCCATG

GCTACTTATGGTTGGAATTTGGTCAAACTCCATTCAGGGGTTGACGTGTTCTACAAACCCACAGAGCAAG

TGGACACCCTGCTCTGTGATATTGGGGAGTCATCTTCTAATCCAACAATAGAGGAAGGAAGAACATTAAG

AGTTTTGAAGATGGTGGAGCCATGGCTCTCTTCAAAACCTGAATTCTGCATCAAAGTCCTTAACCCCTAC

ATGCCAACAGTCATAGAAGAGCTGGAGAAACTGCAGAGAAAACATGGTGGGAACCTTGTCAGATGCCCGC

TGTCCAGGAACTCCACCCATGAGATGTATTGGGTGTCAGGAGCGTCGGGAAACATTGTGAGCTCTGTGAA

CACAACATCAAAGATGTTGTTGAACAGGTTCACAACAAGGCATAGGAAACCCACTTATGAGAAGGACGTA

GATCTTGGGGCAGGAACGAGAAGTGTCTCCACTGAAACAGAAAAACCAGACATGACAATCATTGGGAGAA

GGCTTCAGCGATTGCAAGAAGAGCACAAAGAAACCTGGCATTATGATCAGGAAAACCCATACAGAACCTG

GGCGTATCATGGAAGCTATGAAGCTCCTTCGACAGGCTCTGCATCCTCCATGGTGAACGGGGTGGTAAAA

CTGCTAACAAAACCCTGGGATGTGATTCCAATGGTGACTCAGTTAGCCATGACAGATACAACCCCTTTTG

GGCAACAAAGAGTGTTCAAAGAGAAGGTGGATACCAGAACACCACAACCAAAACCCGGTACACGAATGGT

TATGACCACGACAGCCAATTGGCTGTGGGCCCTCCTTGGAAAGAAGAAAAATCCCAGACTGTGCACAAGG

GAAGAGTTCATCTCAAAAGTTAGATCAAACGCAGCCATAGGCGCAGTCTTTCAGGAAGAACAGGGATGGA

CATCAGCCAGTGAAGCTGTGAATGACAGCCGGTTTTGGGAACTGGTTGACAAAGAAAGGGCCCTACACCA

GGAAGGGAAATGTGAATCGTGTGTCTATAACATGATGGGAAAACGTGAGAAAAAGTTAGGAGAGTTTGGC

AGAGCCAAGGGAAGCCGAGCAATCTGGTACATGTGGCTGGGAGCGCGGTTTCTGGAATTTGAAGCCCTGG

GTTTTTTGAATGAAGATCACTGGTTTGGCAGAGAAAATTCATGGAGTGGAGTGGAAGGGGAAGGTCTGCA

CAGATTGGGATATATCCTGGAGGAGATAGACAAGAAGGATGGAGACCTAATGTATGCTGATGACACAGCA

GGCTGGGACACAAGAATCACTGAGGATGACCTTCAAAATGAGGAACTGATCACGGAACAGATGGCTCCCC
ACCACAAGATCCTAGCCAAAGCCATTTTCAAACTAACCTATCAAAACAAAGTGGTGAAAGTCCTCAGACC

CACACCGCGGGGAGCGGTGATGGATATCATATCCAGGAAAGACCAAAGAGGTAGTGGACAAGTTGGAACA

TATGGTTTGAACACATTCACCAACATGGAAGTTCAACTCATCCGCCAAATGGAAGCTGAAGGAGTCATCA

CACAAGATGACATGCAGAACCCAAAAGGGTTGAAAGAAAGAGTTGAGAAATGGCTGAAAGAGTGTGGTGT

CGACAGGTTAAAGAGGATGGCAATCAGTGGAGACGATTGCGTGGTGAAGCCCCTAGATGAGAGGTTTGGC

ACTTCCCTCCTCTTCTTGAACGACATGGGAAAGGTGAGGAAAGACATTCCGCAGTGGGAACCATCTAAGG

GATGGAAAAACTGGCAAGAGGTTCCTTTTTGCTCCCACCACTTTCACAAGATCTTTATGAAGGATGGCCG

CTCACTAGTTGTTCCATGTAGAAACCAGGATGAACTGATAGGGAGAGCCAGAATCTCGCAGGGAGCTGGA

TGGAGCTTAAGAGAAACAGCCTGCCTGGGCAAAGCTTACGCCCAGATGTGGTCGCTTATGTACTTCCACA

GAAGGGATCTGCGTTTAGCCTCCATGGCCATATGCTCAGCAGTTCCAACGGAATGGTTTCCAACAAGCAG

AACAACATGGTCAATCCACGCTCATCACCAGTGGATGACCACTGAAGATATGCTCAAAGTGTGGAACAGA

GTGTGGATAGAAGACAACCCTAATATGACTGACAAGACTCCAGTCCATTCGTGGGAAGATATACCTTACC

TAGGGAAAAGAGAGGATTTGTGGTGTGGATCCCTGATTGGACTTTCTTCCAGAGCCACCTGGGCGAAGAA

CATTCATACGGCCATAACCCAGGTCAGGAACCTGATCGGAAAAGAGGAATACGTGGATTACATGCCAGTA

ATGAAAAGATACAGTGCTCCTTCAGAGAGTGAAGGAGTTCTGTAATTACCAACAACAAACACCAAAGGCT

ATTGAAGTCAGGCCACTTGTGCCACGGTTTGAGCAAACCGTGCTGCCTGTAGCTCCGCCAATAATGGGAG

GCGTAATAATCCCCAGGGAGGCCATGCGCCACGGAAGCTGTACGCGTGGCATATTGGACTAGCGGTTAGA

GGAGACCCCTCCCATCACTGATAAAACGCAGCAAAAGGGGGCCCGAAGCCAGGAGGAAGCTGTACTCCTG

GTGGAAGGACTAGAGGTTAGAGGAGACCCCCCCAACACAAAAACAGCATATTGACGCTGGGAAAGACCAG

AGATCCTGCTGTCTCTGCAACATCAATCCAGGCACAGAGCGCCGCAAGATGGATTGGTGTTGTTGATCCA

ACAGGTTCT
Whole genome alignment
CLC Sequence Viewer 6 Multiple Alignment
AG T T G T T AG T C T A CG T GGA C CGA C A AGA A C AG T T T CGA A T CGGA AGC T T -
AG T T G T T AG T C T A CG T GGA C CGA C A AGA A C AG T T T CGA C T CGGA AGC T T -
AG T T G T T AG T C T G T G T GGA C CGA C A AGGA C AG T T C C A A A T CGGA AGC T T -
AG T T G T T AG T C T A CG T GGA C CGA C A A AGA C AGA T T C T T T G AGGGAGC T A A

GC T T A A CG T A G T T C T A A C AG T T T T T T - - AT T AGAGAGC AG A T C T C T GA T G
GC T T A A CG T A G T GC T GA C AG T T T T T T - - AT T AGAGAGC AG A T C T C T GA T G
GC T T A A C A C A G T T C T A A C AG T T T G T T T GA A T AGAGAGC AG A T C T C T GGA A
GC T C A A CG T A G T T C T A A C AG T T T T T T - AA T T AGAGAGC AG A T C T C T GA T G

AAC - - AACCA A CGGA A A A AG A CGGG T CGA C CG T C T T T C A A T A T GC T GA A A

AAC - - AACCA A CGGA AGA AG A CGGGA A A A C CG T C T A T C A A T A T GC T GA A A
A A A T GA A C C A A CGA A A A A AG G T GG T T AGA C CACC T T T CAA T A T GC T GA A A
AA T - - AACCA A CGGA A A A AG GCGA A A A A C A CGC C T T T C A A T A T GC T GA A A

CGCGCGAGA A A C CGCG T G T C AAC TGT T T CA C AG T T GGCGA AGAGA T T C T C

CGCG T GAGA A A C CG T G T G T C A A C T GGA T C A C AG T T GGCGA AGAGA T T C T C
CGCGAGAGA A A C CGCG T A T C AACCCC T CAA GGG T T GG T GA AGAGA T T C T C
CGCGAGAGA A A C CGCG T G T C GA C T G T GC A A C AGC T GA C A A AGAGA T T C T C

A A A AGGA T T G C T T T C AGGC C A AGGA C C C A T GA A A T T GG T G A T GGC T T T T A

A A A AGGA C T G C T GA A CGGC C AGGGA C C A A T GA A A T T GG T T A T GGCG T T C A
A A C CGGA C T T T T T T C T GGGA A AGGA C C C T T A CGGA T GG T G C T AGC A T T C A
A C T T GGA A T G C T GC AGGGA C GAGGA C C A T T A A A A C T G T T C A T GGC C C T GG

T AGC A T T C C T A AGA T T T C T A GC C A T A C C T C C A A C AGC AGG A A T T T T GGC T

T AGC T T T C C T C AGA T T T C T A GC C A T T C C A C C A A C AGC AGG AG T C T T GGC T
T C A CG T T T T T GCGAG T C C T T T CCA T CCCAC C A A C AGC AGG GA T T C T GA AG
T GGCG T T C C T T CG T T T C C T A ACAA T CCCAC C A A C AGC AGG GA T A T T GA AG

AGA T GGGGC T C A T T C A AGA A GA A T GGAGCG A T C A A AG T G T T A CGGGG T T T

AGA T GGGGA A C C T T C A AGA A G T CGGGGGC C A T T A AGG T C C T GA A AGGC T T
AGA T GGGGA C AG T T GA AGA A A A A T A AGGC C A T C A AGA T A C T GA T T GGA T T
AGA T GGGGA A CAA T T AAAAA A T C A A A AGC T A T T AA TGT T T T GAGAGGG T T

C A AGA A AGA A A T C T CAAACA T G T T GA A C A T A A T GA A C AGG AGGA A A AGA T

C A AGA AGGAG A T C T CAAACA T GC T GAGC A T AA T CAACCAA CGGA A A A AGA
C AGGA AGGAG A T AGGC CGC A T GC T GA A C A T C T T GA A CGGG AGA A A A AGG T
C AGGA A AGAG A T T GGA AGGA T GC T GA A C A T C T T GA A T AGG AGA CGC AGA T

C T G T GA C C A T GC T C C T C A T G C T GC T GC C C A C AGC C C T GGC GT T CCA T C TG

C A T CGC T C T G T C T C A T GA T G A T A T T GC C AG C AGC A C T T GC T T T CCAC T TG
C A A CGA T A A C A T T GC T G T GC T T GA T T C C C A C CG T A A T GGC GT T T T CCC T C
C T GC AGGC A T GA T C A T T A T G C T GA T T C C A A C AG T GA T GGC GT T CCA T T T A

A C C A C C CGAG GGGGAGAGC C GC A C A T GA T A G T T AGC A AGC AGGA A AGAGG

A C T T C A CGAG A T GGAGAGC C GCGC A T GA T T G T GGGGA AGA A T GA A AGAGG
AGC A C A AGAG A T GGCGA A C C C C T C A T GA T A G T GGC A A A A C A T GA A AGGGG
A C C A C A CG T A A CGGAGA A C C A C A C A T GA T C G T C AGC AGA C A AGAGA A AGG

1
AAAA T CAC T T T T G T T T A AGA C C T C T GC AGG TGT CAACA TG T GC A C C C T T A
T AAA T CCC T A C T T T T T A AGA C AGC C T C T GG AA T CAACA TG T GC A C A C T C A
GAGA C C T C T C T T G T T T A AGA C A A C AGAGGG GA T C A A C A A A T GC A C T C T C A
GA A A AG T C T T C TGT T T AAAA C AGAGGA T GG CG T GA A C A T G TGT ACCC T CA

T T GC A A T GGA T T T GGGAGAG T T A T G T GAGG A C A C A A T GA C C T A C A A A T GC

T AGC C A T GGA T T T GGGAGAG A T G T G T GA T G A C A CGG T C A C T T A C A A A T GC
T T GC C A T GGA C T T GGG T GA A A T G T G T GAGG ACAC TGT CAC G T A T A A A T GC
T GGC C A T GGA C C T T GG T GA A T T G T G T GA AG ACACAA T CAC G T A C A AG T G T

C C C CGGA T C A C T GAGA CGGA A C C AGA T GA C G T T GA C T G T T GG T GC A A T GC

CCCCACA T T A C CGA AG T GGA A C C T GA AGA C A T T GA C T GC T GG T GC A A C C T
C C C C T A C T GG T C A A T A C CGA A C C T GA AGA C A T T GA T T GC T GG T GC A A C C T
CCCC T T C T CA GGC AGA A T GA GC C AGA AGA C A T AGA C T G T T GG T GC A A C T C

C A CGGAGA C A T GGG T GA C C T A T GGA A C A T G T T C T CAAAC T GG T GA A C A C C

T ACA T CAACA T GGG T GA C T T A T GGA A CG T G C A A T C A AGC T GGAGAGC A T A
C A CG T C T A C C T GGG T C A T G T A T GGGA C A T G C A C C C AGAGC GGAGA A CGGA
T A CG T C C A CG T GGG T A A C T T A T GGGA CG T G T ACCACCA TG GGAGA A C A T A

GA CGAGA C A A A CG T T C CG T C GC A C T GGC A C C A C A CG T AGG GC T T GG T C T A

GA CGCGA C A A GAGA T C AG T G GCG T T AGC T C C C C A T G T CGG C A T GGGA C T G
GA CGAGAGA A GCGC T C AG T A GC T T T A A C A C C A C A T T C AGG A A T GGGA T T G
GA AGAGA A A A A AGA T C AG T G GC A C T CG T T C C A C A T G T GGG A A T GGGA C T G

GA A A C A AGA A C CGA A A CG T G GA T G T C C T C T GA AGGCGC T T GGA A A C A A A T

GA C A C A CGC A CCCAAACC TG GA T G T CGGC T GA AGGAGC T T GGAGA C A AG T
GA A A C A AGAG C T GAGA C A T G GA T G T C A T CG GA AGGGGC T T GGA AGC A T GC
GAGA C A CGA A C T GA A A C A T G GA T G T C A T C A GA AGGGGC C T GGA A A C A T G T

A C A A A A AG T G GAGA C C T GGG C T C T GAGA C A C C C AGGA T T C A CGG T GA T AG

CGAGA AGG T A GAGA C A T GGG C C C T T AGGC A C C C AGGG T T C A C C A T A C T AG
T C AGAGAG T A GAGAGC T GGA T A C T C AGA A A C C C AGGA T T C GCGC T C T T GG
C C AGAGA A T T GA A A C T T GGA T C T T GAGA C A T C C AGGC T T C A C C A T GA T GG

C C C T T T T T C T AGC A C A T GC C A T AGGA A C A T CCA T CACCCA GA A AGGGA T C

C C C T A T T T C T CGC C C A T T A C A T AGGC A C T T C C C T GA C C C A GA AGG T GG T T
C AGGA T T T A T GGC T T A T A T G A T T GGGC A A A C AGGA A T C C A GCGA A C T G T C
C AGC A A T C C T GGC A T A C A C C A T AGGA A CGA CACA T T T CCA A AGAGC C C T G

AT T T T TAT T T T GC T GA T GC T GG T A A C T C C A T C C A T GGC C A T GCGG T GCG T

A T T T T CA T A T T A T T A A T GC T GG T C A C C C C A T C C A T GA C A A T GAGA T G T G T
T T C T T TGT CC T A A T GA T GC T GG T CGC C C C A T C C T A CGGA A T GCGA T GCG T
A T T T T CA T C T T A C T GA C AGC TGT CAC T CC T T C A A T GA C A A T GCG T T GC A T

GGGA A T AGGC A A C AGAGA C T T CG T GGA AGG A C T G T C AGGA GC T A CG T GGG

GGGAG T AGGA A A C AGAGA T T T T G T GGA AGG GC T A T C AGGA GC T A CG T GGG
AGGAG T AGGA A A C AGAGA C T T T G T GGA AGG AG T C T C AGG T GGAGC A T GGG
AGGA A T G T C A A A T AGAGA C T T T G T GGA AGG GG T T T C AGGA GGA AGC T GGG

2
T GGA T G T GG T A C T GGAGC A T GGA AG T T GCG T CAC T ACCA T GGC A A A AGA C
T T GA CG T GG T GC T CGAGC A C GGGGGG T G T G T GA C T A C C A T GGC T A AGA A C
T CGA C C T GG T GC T AGA A C A T GGAGGA T GCG T CACAACCA T GGC C C AGGGA
T T GA C A T AG T C T T AGA A C A T GGA AGC T G T G T GA CGA CGA T GGC A A A A A A C

AAACCAACAC T GGA C A T T GA A C T C T T GA AG A CGGAGG T C A C A A A C C C T GC

A AGC C C A CGC T GGA T A T AGA GC T T C AGA AG A C CGAGGC C A C C C A A C T GGC
AAACCAACC T T GGA T T T T GA A C T GA C T A AG A C A A C AGC C A AGGA AG T GGC
AAACCAACA T T GGA T T T T GA A C T GA T A A A A A C AGA AGC C A A A C AGC C T GC

CG T C C T GCGC A A A C T G T GC A T T GA AGC T A A AA T A T CAAAC A C C A C C A C CG

GA C C C T A AGG A AGC T A T GC A T T GAGGGGA A AA T T ACCAAC A T AACAAC TG
T C T G T T A AGA A C C T A T T GC A T T GA AGC C T C AA T A T CAAAC A T A A C T A CGG
C A C C C T A AGG A AG T A C T G T A T AGAGGC A A A GC T A A C C A A C A C A A C A A C AG

A T T CGAGA T G T CCAACACAA GGAGA AGC C A CGC T GG T GGA AGA A C AGGA C

A C T C A AGA T G T CC T ACCCAA GGGGA AGCGG T T T T GC C T GA GGAGC AGGA C
C A A C A AGA T G T C C A A CGC A A GGAGAGC C T T A T C T GA A AGA GGA A C AGGA C
A A T C T CGC T G CCCAACACAA GGGGA A C C C A GC C T A A A T GA AGAGC AGGA C

A CGA A C T T T G T G T G T CGA CG A A CG T T CG T G GA C AGAGGC T GGGGC A A T GG

C AGA A C T A CG T G T G T A AGC A T A C A T A CG T A GA C AGAGG T T GGGGGA A CGG
C A A C AG T A C A T T T GC CGGAG AGA T G T GG T A GA C AGAGGG T GGGGC A A T GG
A A A AGG T T CG T C T GC A A A C A C T C C A T GG T A GA C AGAGGA T GGGGA A A T GG

T T G T GGGC T A T T CGGA A A AG G T AGC T T A A T A A CG T G T GC T A AG T T T A AG T

T T G T GG T T T G T T T GGC A A AG GA AGC T T GG T A A C A T G T GCG AAA T T T CAA T
C T G T GGC T T G T T T GGA A A AG GAGGAG T T G T GA C A T G T GCG A AG T T T T C A T
A T G T GGA C T A T T T GGA A AGG GAGGC A T T G T GA C C T G T GC T A T G T T C AGA T

G T G T GA C A A A A C T GGA AGGA A AGA T AG T C C A A T A T GA A A A C T T AAAA T A T

GC C T GGA A C C A A T AGAGGGA A A AG T GG T GC A A T A T GAGA A CC T CAAA T AC
G T T CGGGGA A GA T A A C AGGC A A T T T GG T C C A A A T T GAGA A C C T T GA A T A C
GC A A A A AGA A C A T GGA AGGA A A AG T T G T GC A A C C AGA A A A C T T GGA A T A C

T C AG T GA T AG T C A C CG T A C A C A C T GGAGA C C AGC A C C A AG T T GGA A A T GA

A C CG T C A T C A T T A C AG T GC A C A C AGGAGA C C A A C A C C AGG T GGGA A A T GA
A C AG T GG T T G T A A C AG T C C A C A A T GGAGA C A C C C A T GC AG T AGGA A A T GA
A C C A T T G T GA T AACACC T CA C T C AGGGGA A GAGC A T GC AG T CGGA A A T GA

GA C C A C AGA A C A T GGA A C A A C T GC A A C C A T AACACC T CAA GC T C C C A CG T

AAC - - - - - - G C A AGGAG T C A CGGC T GAGA T A A C A C C T C AG GC A T C A A C C A
CACA T CCAA T C A T GGAG T T A C AGC C A T GA T A A C T C C C AGG T C A C C A T CGG
C A C AGGA A A A C A T GGC A AGG AAA T CAAAA T A A C A C C A C AG AG T T C C A T C A

CGGA A A T A C A GC T GA C AGA C T A CGGAGC T C T A A C A T T GGA T TGT T CACC T

C T GA AGC C A T C T T GC C T GA A T A T GGA A C C C T T GGGC T AGA A T GC T C A C C A
T GGA AG T C A A A T T GC CGGA C T A T GGAGA A C T A A C A C T CGA T T G T GA A C C C
C AGA AGC AGA A T T GA C AGG T T A T GGC A C T G T C A C A A T GGA G T GC T C T C C A

3
AGA A C AGGGC T AGA C T T T A A T GAGA T GG T G T T G T T GA C A A T GA A A A A A A A
CGGA C AGG T T T GGA T T T C A A T GA A A T GA T C T T AC T AACAA T GA AGA A C A A
AGG T C T GGA A T T GA C T T T A A T GAGA T GA T T C T GA T GA A A A T GA A A A AGA A
AGA A CGGGC C T CGA C T T C A A T GAGA T GG T G T T GC T GC AGA T GGA A A A T A A

A T C A T GGC T C GT CCACAAAC A A T GG T T T C T AGA C T T A C C A C T GC C T T GGA

AGC A T GGA T G G T A C A T AGA C A A T GG T T C T T T GA C C T A C C T C T A C C A T GGG
A A C A T GGC T C G T GC A T A AGC A A T GG T T T T T GGA T C T GC C T C T T C C A T GGA
AGC T T GGC T G G T GC A C AGGC A A T GG T T C C T AGA C C T GC CG T T A C C A T GG T

C C T CGGGGGC T T CAACA T CC C A AGAGA C T T GGA A T AGA C A AGA C T T GC T G

C A T C AGGAGC T A C A A C AGA A ACACCAACC T GGA A C AGGA A GGAGC T T C T T
C AGC AGGAGC AGA C A C A T C A GAGG T T C A C T GGA A T T A C A A AGAGAGA A T G
T GC C CGGAGC GGA C A C A C A A GGG T C A A A T T GGA T A C AGA A AGAGA C A T T G

GT CACA T T T A AGA C AGC T C A T GC A A A A A AG C AGGA AG T AG T CG T A C T AGG

G T GA C A T T C A A A A A CGC A C A T GCGA A A A A A C A AGA AG T AG T T G T C C T T GG
G T GA C A T T T A AGG T T C C T C A T GC C A AGAGA C AGGA T G T GA C AG T GC T GGG
GT CAC T T T CA AAAA T CCCCA T GCGA AGA A A C AGGA T G T T G T T G T T T T AGG

A T C A C A AGA A GGAGC A A T GC A C A C T GCG T T GA C T GGAGCG A C AGA A A T C C

A T CGC A AGAG GGAGC A A T GC A T A C CGC A C T GA C AGGAGC T A C AGA A A T C C
A T C T C AGGA A GGAGC C A T GC A T T C T GC C C T CGC T GGAGC C A C AGA AG T GG
A T C C C A AGA A GGGGC C A T GC A C A C AGC A C T T A C AGGGGC C A C AGA A A T C C

A A A CG T C T GG A A CGA C A A C A A T T T T T GC AG GA C A C C T GA A A T GC AGA T T A
A A A A C T C AGG AGGC A C A AGC A T T T T CGCGG GGC A C T T A A A A T G T AGA C T T
A C T C CGG T GA T GGA A A T C A C A T G T T T GC AG GA C A T C T T A A G T GC A A AG T C
AAA TGT CA T C AGGA A A C T T A C T C T T C A C AG GA C A T C T C A A G T GC AGGC T G

A A A A T GGA T A A A C T GA T T T T A A A AGGGA T G T CA T A TGT AA T G T GC A C AGG

A AGA T GGA C A A A T T GGA A C T C A AGGGGA T G AGC T A T GC A A T G T GC A CGA A
CG T A T GGAGA A A T T GAGA A T C A AGGGA A T G T C A T A C A CGA T G T G T T C AGG
AGA A T GGA C A AGC T A C AGC T C A A AGGA A T G T CA T AC T C T A T G T GC A C AGG

G T C A T T C A AG T T AGAGA AGG A AG T GGC T GA GA C C C AGC A T GGA A C T G T T C

T ACC T T TGTG T T GA AGA A AG A AG T C T C AGA A A CGC AGC A C GGGA C A A T A C
A A AG T T T T C A A T T GA C A A AG AGA T GGC AGA A A C A C AGC A T GGGA C A A C AG
A A AG T T T A A A G T T G T GA AGG A A A T AGC AGA AACACAACA T GGA A C A A T AG

T AG T GC AGG T T A A A T A CGA A GGA A C AGA T G C A C C A T GC A A GA T C C C C T T C

T C A T T A AGG T T GAG T A C A A A GGGGA AGA T G C A C C T T GC A A GA T T C C C T T T
T GG T GA A AG T C A AG T A T GA A GG T GC T GGAG C T C CG T G T A A AG T C C C C A T A
T T A T C AGAG T GC A A T A T GA A GGGGA CGGC T C T C C A T GC A A GA T C C C T T T T

T CG T C C C A AG A T GAGA AGGG AG T A A C C C AG A A T GGGAGA T T GA T A A C AGC

T C C A C AGAGG A T GGA C A AGG GA A AGC T C A T A A T GGC AGA C T GA T C A C AGC
GAGA T A AGAG A TGT AAACAA GGA A A A AG T G G T T GGGCG T A T CA T C T CA T C
GAGA T A A T GG A T T T GGA A A A A AGA C A T G T C T T AGG T CGC C T GA T T A C AG T

4
CAACCCCA T A G T C A C T GA C A A AGA A A A A C C AG T C A A C A T T GA AGCGGAGC
CAACCC TGTG G T GA C T A AGA AGGAGGAGC C TGT CAA T A T T GAGGC T GA A C
CACCCC T T TG GC T GAGA A T A C C A A C AG T G T AACCAACA T A GA A T T AGA A C
CAACCCAA T T G T GA C AGA A A A AGA T AGC C C AG T C A A C A T A GA AGC AGA A C

C A C C T T T T GG T GAGAGC T A C A T T G T GG T AG GAGC AGG T GA A A A AGC T T T G

C T C C T T T T GG GGA A AGC A A T A T AG T A A T T G GA A T T GGAGA C A A CGC C T T G
C C C C C T T T GG GGA C AGC T A C A T AG T GA T AG G T G T T GGA A A C AGCGC A T T A
C T C C A T T CGG AGA C AGC T A C A T C A T C A T AG GAG T AGAGC C GGGA C A A C T G

A A A C T A AGC T GG T T C A AGA A GGGA AGC AG T A T AGGGA A A A T G T T T GA AGC

AAAA T CAAC T GG T A C A AGA A GGGGAGC T CG A T T GGGA AGA T G T T CGAGGC
ACAC T CCA T T GG T T C AGGA A AGGGAG T T C C A T T GGC A AGA T G T T T GAG T C
A AGC T C A A C T GG T T T A AGA A AGGA AG T T C T A T CGGC C A A A T G T T T GAGA C

A A C T GC C CG T GGAGC A CGA A GGA T GGC C A T C C T GGGAGA C A C T GC A T GGG

C A C T GA A AGG GG T GC A AGGC GC A T GGC C A T C T T GGGAGA C A C AGC T T GGG
C A C A T A C AGA GG T GC A A A A C GA A T GGC C A T T C T AGG T GA A A C AGC T T GGG
A A C A A T GAGG GGGGCGA AGA GA A T GGC C A T T T T AGG T GA C A C AGC C T GGG

A C T T CGG T T C T A T AGGAGGG G T G T T C A CG T C T G T GGGA A A A C T GA T A C A C

A C T T T GGA T C AG T GGG T GG T G T T C T GA A C T C A T T AGGC A A A A T GG T GC A C
A T T T T GG T T C CG T T GG T GGA C TGT T CACA T C A T T GGGA A A GGC T G T GC A C
A T T T T GGA T C C T T GGGAGGA GTGT T T ACA T C T A T AGGA A A GGC T C T C C A C

C AGA T T T T T G GGA C T GCG T A T GGAG T T T T G T T C AGCGG T G T T T C T T GGA C

CAAA T A T T TG GA AG T GC T T A T A C AGC C C T G T T C AG T GGAG T C T C T T GGG T
C AGG T T T T T G GA AG T G T G T A T ACAACCA TG T T T GGAGGAG T C T C A T GGA T
C A AG T C T T T G GAGC A A T C T A T GGAGC T GC C T T C AG T GGGG T T T C A T GGA C

C A T GA AGA T A GGA A T AGGGA T T C T GC T GA C A T GGC T AGGA T T AAAC T CAA

GA T GA A A A T T GGA A T AGG T G T C C T C T T GA C T T GGA T AGGG T T GA A T T C A A
GA T T AGA A T C C T A A T T GGG T T C T T AG T G T T G T GGA T T GGC A CGA A C T CGA
T A T GA A A A T C C T C A T AGGAG T CA T T A T CAC A T GGA T AGGA A T GA A T T C A C

GGAGC A CG T C CC T T T CAA TG A CG T G T A T CG C AG T T GGC A T GG T C A C A C T G

AAAACACA T C CA TGT CA T T T T C A T GC A T T G CGA T AGGA A T CA T T ACAC T C
GGA A C A C T T C A A T GGC T A T G A CG T GC A T AG C T G T T GGAGG AA T CAC T C TG
GC AGC A C C T C AC TGT C TGTG A C A C T AG T A T T GG T GGGA A T T G T GA C A C T G

T A C C T AGGAG T C A T GG T T C A GGCGGA C T CG GGA T G T G T A A T C A A C T GGA A

T A T C T GGGAG C T G T GG T A C A AGC T GA C A T G GGG T G T G T C A T A A A C T GGA A
T T T C T GGGC T T C A C AG T T C A AGC AGA C A T G GG T T G T G T GG CG T C A T GGAG
T A T T T GGGAG T C A T GG T GC A GGC CGA T AG T GG T T GCG T T G T GAGC T GGA A

AGGC AGAGA A C T CAAA TGTG GA AGCGGC A T T T T TGT CACC A A T GA AG T C C

GGGC A A AGA A C T CAAA TGTG GA AGCGGA A T T T T CG T C A C C A A T GAGG T C C
T GGGA A AGA A T T GA AG T G T G GA AGCGGA A T T T T T G T GG T T GA C A A CG T GC
A A A C A A AGA A C T GA A A T G T G GC AG T GGGA T T T T CA T CACA GA C A A CG T GC

5
A C A C C T GGA C AGAGC A A T A T A A A T T C C AGG C CGA C T C C C C T A AGAGA C T A
A T A C C T GGA C AGAGC A A T A C A A A T T C C A AG C AGA C T C C C C A A A A AGA T T G
A C A C T T GGA C AGA A C AG T A C AAA T T T CAAC C AGAG T C C C C AGCGAGA C T A
A C A C A T GGA C AGA A C A A T A C A AG T T C C A A C C AGA A T C C C C T T CAAAAC T A

T C AGCGGC C A T T GGGA AGGC A T GGGAGGAG GG T G T G T G T G GA A T T CGA T C

GC A A C AGC C A T T GC AGGCGC C T GGGAGA A T GGAG T G T G T G GA A T T AGG T C
GCG T C T GC A A T A T T A A A T GC C C A C A A AGA T GGGG T C T G T G GA A T T AGA T C
GC T T C AGC T A T C C AGA A AGC C C A T GA AGAG GGC A T T T G T G GA A T C CGC T C

AGC C A C T CG T C T CGAGA A C A T C A T G T GGA A GC A A A T A T C A A A T GA A T T A A

A A C A A C C AGA A T GGAGA A T C T C T T G T GGA A GC A A A T AGC C A A T GA A C T GA
A A C C A CGAGG C T GGA A A A T G T C A T G T GGA A GC A A A T A A C C A A CGAGC T A A
AG T A A C A AGA C T GGAGA A T C T GA T G T GGA A ACAAA T AACA C C AGA A T T GA

ACCACA T C T T A C T T GA A A A T GA C A T GA A A T T T A C AG T GG T CG T AGGAGA C
AC T ACA T A T T A T GGGA A A A C AA T A T CAAA T T A A CGG T AG T T G T GGGCGA T
AC T A TGT T C T C T GGGA AGGA GGA C A T GA C C T C A C T G T AG T GGC T GGGGA T
A T CACA T T C T A T C AGA A A A T GAGG T GA AG T T A A C T A T T A T GA C AGGAGA C

G T T AG T GGA A T C T T GGC C C A AGGA A AGA A A A T GA T T AGGC CACAACCCA T

A C A C T T GGGG T C T T AGAGC A AGGGA A A AGA ACAC T AACAC CACAACCCA T
G T GA AGGGGG T G T T GA C C A A AGGC A AGAGA GC A C T C A C A C C C C C AG T GAG
A T C A A AGGA A T C A T GC AGGC AGGA A A A CGA T C T C T GCGGC C T C AGC C C A C

GGA A C A C A A A T A C T CG T GGA A A AGC T GGGG A A A AGC C A A A A T C A T AGGAG

GGAGC T A A A A T A C T C A T GGA A A A CG T GGGG A A AGGC A A A A A T AG T GA C AG
T GA T C T GA A A T A T T C A T GGA AGA C A T GGGG A A A AGC A A A A A T C T T CACCC
T GAGC T GA AG T A T T C A T GGA A A A C A T GGGG C A A AGC A A A A A T GC T C T C T A

C AGA T G T A C A GA A T A C C A C C T T C A T C A T CG A CGGC C C A A A C A C C C C AGA A

C T GA A A C A C A AAA T T CC T C T T T C A T A A T AG A CGGGC C A A A C A C A C CGGAG
C AGA AGC A AG A A A T AGC A C A T T T T T A A T AG A CGGA C C AGA C A C C T C T GA A
C AGAG T C T C A T A A C C AGA C C T T T C T CA T TG A T GGC C C CGA A A C AGC AGA A

T GC C C T GA T A A C C A A AGAGC A T GGA A C A T T T GGGA AG T T G A AGA C T A T GG

T G T C C A AG T G C C T C A AGAGC A T GGA A T G T G T GGGAGG T GG A AGA T T A CGG
T GC C C C A A T G A A CGA AGAGC A T GGA A C T C T C T T GAGG T GG A AGA C T A T GG
T GC C C C A A C A C A A A T AGAGC T T GGA A T T CG T T GGA AG T T G A AGA C T A T GG

A T T T GGA A T T T T C A CGA C A A A C A T A T GG T T GA A A T T GCG T GA C T C C T A C A

G T T CGGAG T C T T CACAACCA A C A T A T GGC T GA A A C T C CGA GAGG T C T A C A
A T T T GGC A T G T T C A CGA C C A A C A T A T GGA T GA A A T T C CGA GA AGGA AG T T
C T T T GGAG T A T T CACCACCA A T A T A T GGC T A A A A T T GA A A GA A A A A C AGG

C T C A AG T G T G T GA C C A C CGG C T A A T G T C AG C T GC C A T C A A GGA T AGC A A A

CCCAAC T A TG T GA C C A T AGG C T A A T G T CGG C AGC T G T C A A GGA T GAGAGG
C AGA AG T G T G T GA C C A C AGG T T A A T G T C AG C T GC A A T T A A AGA T C AGA A A
ATGTAT TCTG CGA C T C A A A A C T C A T G T C AG CGGC C A T A A A AGA C A A C AGA

6
GC AG T C C A T G C T GA C A T GGG G T A C T GGA T A GA A AG T GA A A AGA A CGAGA C
GC CG T GC A T G C CGA C A T GGG C T A C T GGA T A GA A AGC C A A A AGA A T GGA AG
GC T G T GC A T G C T GA C A T GGG T T A T T GGA T A GAGAGC T C A A A A A A C C AGA C
GC CG T C C A T G C CGA T A T GGG T T A T T GGA T A GA A AG T GC A C T C A A T GA C A C

T T GGA AG T T G GC A AGAGC C T C C T T C A T AGA AG T T A AGA C A T GC A T C T GGC

T T GGA AGC T A GA A A A AGC A T C C C T C A T AGA GG T A A A A A C C T GC A C A T GGC
C T GGC AGA T A GAGA A AGC A T C T C T T A T T GA AG T GA A A A C A T G T C T G T GGC
A T GGA AGA T A GAGA A AGC C T C T T T C A T T GA AG T T A A A A A C T GC C A C T GGC

CAAAA T CCCA C A C T C T A T GG AGC A A T GGAG T C C T GGA A AG T GAGA T GA T A

CAAAA T CACA C A C T C T C T GG A C T A A T GG T G T GC T AGAGAG T GA C A T GA T C
C C A AGA C C C A C A C A C T G T GG AGC A A T GGAG T GC T GGA A AG C C AGA T GC T C
CAAAA T CACA C A C C C T C T GG AGC A A T GGAG T GC T AGA A AG T GAGA T GA T A

A T C C C A A AGA T A T A T GGAGG ACCAA T A T C T C AGC A C A A C T A C AGA C C AGG

A T C C C A A AGA G T C T AGC T GG T CC T A T C T CA CAACACAAC T A C AGGC C CGG
A T T CCAAAA T C A T A T GCGGG CCC T T T T T CA C AGC A C A A T T A C CGC C AGGG
A T T C C A A AGA A T C T CGC T GG A C C AG T G T C T CAACACAAC T A T AGA C C AGG

A T A T T T CACA C A A A C AGC AG GGC CG T GGC A C T T GGGC A AG T T AGA A C T AG

GT ACCACACC C A A A CGGC AG GA C C C T GGC A C T T AGGA A A A T T GGAGC T GG
C T A T GC C A CG C A A A C CG T GG GC C C A T GGC A C T T AGGC A A A T T AGAGA T AG
C T ACCA T ACA C A A A T A A C AG GA C C A T GGC A T C T AGG T A AG C T T GAGA T GG

A T T T T GA T T T A T G T GA AGG T ACCAC TGT TG T T G T GGA T GA A C A T T G T GGA

AC T T CAAC T A C T G T GA AGGA A C A A C AG T T G T C A T C A C AGA A AGC T G T GGG
A C T T T GGAGA A T GC C C CGGA A C A A C AG T C A C A A T T C AGGA GGA T T G T GA C
A C T T T GA T T T C T G T GA T GGA A C A A C AG T GG T AG T GA C T GA GGA C T GCGGA

A A T CGAGGA C C A T C T C T T AG AACCACAACA G T C A C AGGA A AGA C A A T C C A

A C A AGAGGC C C A T C A T T GAG AACAACAACA G T G T C AGGGA AG T T GA T A C A
C A T AGAGGC C C A T C T T T GAG GA C C A C C A C T GC A T C T GGA A A A C T AG T C A C
A A T AGAGGA C C C T C T T T GAG AACAACCAC T GC C T C T GGA A AAC T CA T AAC

T GA A T GG T GC T G T AGA T C T T GC A CG T T A C C C C C C C T A CG T T T C A A AGGAG
CGA A T GG T G T T GC CGC T CG T GC A C A C T T C C C C C C C T GCGA T A C A T GGGAG
GC A A T GG T GC T GC CGC T C C T GC A CGA T GC C T C C C T T A AGG T T C T T GGGAG
AGA A T GG T GC T GC CGA T C T T GC A C A T T A C C A C CGC T A AGA T A C AGAGG T G

A AGA CGGG T G C T GG T A CGGC A T GGA A A T C A GA C C AG T C A A GGAGA AGGA A

A AGA CGGC T G C T GG T A T GGC A T GGA A A T C A GA C C C A T C AG T GAGA A AGA A
A AGA T GGG T G C T GG T A T GGG A T GGAGA T T A GGC C C T T GAG T GA A A A AGA A
AGGA T GGG T G C T GG T A CGGG A T GGA A A T C A GA C C A T T GA A GGAGA A AGA A

GAGA A C C T AG T T A AG T C A A T GG T C T C T GC A GGG T C AGGAG A AG T GGA C AG

GAGA A C A T GG T A A AG T C T T T AG T C T C AGCG GGA AG T GGA A AGG T GGA C A A
GAGA A C A T GG T CAAA T CACA GG T GA CGGC C GGA C AGGGC A C A T C AGA A A C
GAGA A T T T GG T CAAC T CC T T GG T C A C AGC T GGA C A T GGGC AGG T CGA C A A

7
T T T T T CAC T A GGA C T GC T A T GC A T A T C A A T A A T GA T CGA A GAGG T A A T GA
C T T CACAA TG GG T G T C T T G T G T T T GGC A A T C C T C T T T GA A GAGG T G T T GA
T T T T TCTATG GG T C T G T T G T GC C T GA C C T T G T T T G T GGA A GA A T GC T T GA
C T T T T CAC T A GGAG T C T T GG GA A T GGC A T T G T T C C T GGAG GA A A T GC T T A

GA T C C AGA T G GAGC AGA A A A A T GC T GA T GA C T GGA A C A T T GGC T G T G T T C

GAGGA A A A T T T GGGA AGA A A C A C A T GA T T G C AGGGG T T T T C T T T A CG T T T
GGAGA AGAG T C A C T AGGA A A C A C A T GA T A T T AG T T G T GG T GA T C A C T C T T
GGA C C CGAG T AGGA A CGA A A C A T GC A A T A C T A C T AG T T GC AG T T T C T T T T

C T CC T T C T CA C A A T GGGA C A A T T GA C A T GG A A T GA T C T GA T C AGGC T A T G
G T GC T C C T T C T C T C AGGGC A A A T A A C A T GG AGAGA C A T GG CGC A C A C A C T
T G T GC T A T C A T C C T GGGAGG C C T C A C A T GG A T GGA C T T A C T A CGAGC C C T
G T GA C A T T GA T C A C AGGGA A CA TGT CC T T T AGAGA C C T GG GA AGAG T GA T

T A T C A T GG T T GGAGC C A A CG C T T C AGA C A A GA T GGGGA T G GGA A C A A CG T

A A T A A T GA T C GGG T C C A A CG C C T C T GA C AG GA T GGGA A T G GGCG T C A C C T
CA T CA TGT TG GGGGA C A C T A T G T C T GG T AG A A T AGGA - - - GGA C AGA T C C
GG T T A T GG T A GGCGC C A C T A T GA CGGA T GA C A T AGG T A T G GGCG T GA C T T

A C C T AGC T T T GA T GGC C A C T T T C AGA A T GA GA C C A A T G T T CGC AG T CGGG

A C C T AGC T C T A A T T GC A A C A T T T AAAA T CC AGC C A T T C T T GGC T T T GGGA
A C C T AGC C A T C A T GGC AG T G T T C A AGA T G T C A C C AGGA T A CG T GC T GGG T
A T C T T GC C C T A C T AGC AGC C T T C A A AG T C A GA C C A A C T T T T GC AGC T GGA

C T AC TGT T T C GC AGA T T A A C A T C T AGAGA A GT TCT TCT TC T T A C AG T T GG

T T T T T CC T AA GA A AGC T GA C A T C T AGAGA A AA T T T A T TGT T AGGAG T T GG
GTGT T T T T AA GGA A A C T C A C T T C A AGAGAG A C AGC A C T A A T GG T A A T AGG
C T A C T C T T GA GA A AGC T GA C C T C C A AGGA A T T GA T GA T GA C T A C T A T AGG

A T T GAG T C T G G T GGC A T C T G T AGA A C T A C C AAA T T CC T T A GAGGAGC T AG

G T T GGC C A T G GC A A C A A CG T T A C A A C T GC C AGAGGA C A T T GA A C A A A T GG
A A T GGC C A T G A C A A CGG T GC T T T CAA T T CC A C A T GA C C T T A T GGA A C T C A
AA T TGT AC T C C T C T C C C AGA GC A C C A T A C C AGAGA C C A T T C T T GAG T T GA

GGGA T GGA C T T GC A A T GGGC A T C A T GA T G T T GA A A T T A C T GA C T GA T T T T

C A A A T GGAG T CGC T C T GGGG C T C A T GGC T C T T A A A C T GA T A A C A C A A T T T
T T GA T GGA A T A T C A C T GGGA C T A A T T T T GC T A A A A A T AG T A A C A C AG T T T
C T GA T GCG T T AGC C T T AGGC A T GA T GG T C C T C A A A A T GG T GAGA A A T A T G

C AG T C A C A T C AGC T A T GGGC T A C C T T GC T G T C T T T AACA T T TGT CAAAAC

GA A A C A T A C C A A T T G T GGA C GGC A T T AG T C T C C T T A A CG T G T T C A A A C A C
GA C A A C A C C C A AG T GGGA A C C T T AGC T C T T T C C T T GA C T T T C A T A AGA T C
GA A A AG T A T C A A T T GGC AG T GA C T A T C A T G GC T A T C T T G T GCG T C C C A A A

AAC T T T T T CA T T GC A C T A T G C A T GGA AGA C A A T GGC T A T G A T AC TGT CAA

A A T T T T T A CG T T GA C T G T T G C C T GGAGA A C AGC C A C T C T G A T T T T GGC CG
A A C A A T GC C A T T GG T C A T GG C T T GGAGGA C C A T T A T GGC T GTGT TGT T TG
CGC AG T GA T A T T A C A A A A CG C A T GGA A AG T GAG T T GC A C A A T A T T GGC AG

8
T TGTATCTCT C T T CCC T T T A T GC C T G T C C A CGA C T T C T C A AAAAA - - - CA
GAG T T T CGC T T T T A C C AG T G T GC C AG T C T T C A AGC A T GAG GA A A A - - - C A
T GG T C A C A C T CA T T CC T T TG T GC AGGA C A A GC T G T C T T C A A A A A C AG T C T
T GG T G T C CG T T T CCCCAC TG C T C - - - T T AA CA T CC T CACA GC A A A A A A C A

A C A T GGC T T C CGG T G T T GC T GGGA T C T C T T GGA T GC A A A C CAC T AACCA T

GA T T GGC T C C C A A T GA C AG T GGC AGC T A T G GGAG T T C C A C CCC T T CCAC T
C A T T GGG T AG A A A T A A C AGC AC T CA T CC T A GGAGC C C A AG C T C T GC C AG T
GA T T GGA T A C C A T T AGC A T T GA CGA T C A A A GG T C T C A A T C C A A C AGC T A T

GT T TCT TATA A C AGA A A A C A A A A T C T GGGG A AGGA A A AGC T GGC C T C T C A

T T T TAT T T T T AGC T T GA A AG ACACAC T CAA A AGGAGA AGC T GGC C A C T GA
GT ACC T AA TG A C T C T T A T GA A AGGAGC C T C A AGA AGA T C T T GGC C T C T T A
T T T T C T AACA ACCC T C T CAA GA A C C AGC A A GA A A AGGAGC T GGC C A T T A A

A T GA AGGA A T T A T GGC T G T T GGA A T AG T T A GC A T T C T T C T A AG T T C A C T T

A T GA AGGGG T GA T GGC T G T T GGGC T T G T GA GC A T T C T GGC C AG T T C T C T C
A CGAGGGC A T A A T GGC T G T G GG T T T GG T T A G T C T C T T AGG A AGCGC T C T T
A T GAGGC T A T C A T GGC AG T C GGGA T GG T GA GC A T T T T AGC C AG T T C T C T C

C T C A AGA A T G A T G T GC C A C T AGC T GGC C C A C T A A T AGC T G GAGGC A T GC T

C T T AGA A A T G A T G T GC C C A T GGC T GGA C C A T T AG T GGC CG GGGGC T T GC T
T T A A AGA A T G A T G T C C C T T T AGC T GGC C C A A T GG T GGC AG GAGGC T T A C T
C T AAAAAA TG A T A T T C C C A T GA C AGGA C C A T T AG T GGC T G GAGGGC T C C T

A A T AGC A T G T T A TGT CA T A T C T GGA AGC T C GGC CGA T T T A T C A C T GGAGA

GA T AGCG T GC T A CG T C A T A A C T GGC A CG T C AGCGGA C C T C A C T G T AGA A A
T C T GGCGGC T T A CG T GA T GA G T GG T AGC T C AGC AGA T C T G T C A C T AGAGA
C A C T G T G T GC T A CG T GC T C A C T GGA CGA T C GGC CGA T T T G GA A C T GGAGA

A AGCGGC T GA GG T C T C C T GG GA AGA AGA AG C AGA A C A C T C T GG T GC C T C A

A AGC C C C AGA T G T A A C A T GG GAGGA AGAGG C T GAGC AGA C AGGAG T G T C C
AGGC CGC C A A CG T GC AG T GG GA T GA A A T GG C AGA C A T A A C AGGC T C A AGC
GAGC AGC CGA T G T C A A A T GG GA AGA C C AGG C AGAGA T A T C AGGA AGC AG T

CACAACA T AC T AG T GGAGG T C C A AGA T GA T GGA A C C A T GA AGA T A A AGGA

CACAAC T T AA T GA T C A C AG T T GA T GA T GA T GGA A C A A T GA GA A T A A A AGA
C C A A T CG T AG A AG T GA AGC A GGA T GA AGA T GGC T C T T T C T C C A T A CGGGA
CCAA T CC TGT CAA T AACAA T A T C AGA AGA T GG T AGC A T G T CGA T A A A A A A

T GA AGAGAGA GA T GA C A C A C T C A C C A T T C T C C T C A A AGC A A C T C T GC T AG
T GA T GAGA C T GAGA A C A T C C T A A C AG T GC T T T T A A A A A C A GC A T T A C T A A
CG T CGAGGA A A C C A A T A T GA T A A C C C T T T T GG T GA A A C T G GC A C T GA T A A
T GA AGAGGA A GA A C A A A C A C T GA C C A T A C T C A T T AGA A C A GGA T T GC T GG

C A A T C T C AGG GG T A T A C C C A A TGT CAA T AC CGGCGA C C C T C T T T G T G T GG

T AG T A T C AGG CA T T T T T CCA T AC T CCA T AC C CGC A A C A T T G T T GG T C T GG
C AG T G T C AGG T C T C T ACCCC T T GGC A A T T C C AG T C A C A A T GA C C T T A T GG
T GA T C T C AGG AC T T T T T CC T GT A T CAA T AC C A A T C A CGGC AGC AGC A T GG

9
T A T T T T T GGC AGA A A A AGA A A C AGAGA T C A GGAG T GC T A T GGGA C A C A C C
C A C A C T T GGC AAAAACAAAC C C A A AGA T C C GGCG T T T T A T GGGA CG T A C C
T A C A T G T GGC A AG T GA A A A C A C A A AGA T C A GGAGC C C T G T GGGA CG T C C C
T A C C T G T GGG A AG T GA AGA A A C A A CGGGC C GGAG T A T T G T GGGA T G T T C C

C AGC C C T C C A GA AG T GGA A A GAGC AG T C C T T GA T GA T GGC A T T T A T AGA A

C AGC C C C C C A GAGA C A C AGA A AGC AGA A C T GGA AGA AGGG G T T T A T AGGA
C T C A C C CGC T GC C A C T A A A A A AGC CGC A C T G T C T GA AGGA G T G T A C AGGA
T T CACCCCCA C C C A T GGGA A AGGC T GA A C T GGA AGA T GGA GC C T A T AGA A

T T C T C C A A AG AGGA T T G T T G GGC AGG T C T C A AG T AGGAG T AGGAG T T T T T

T C A A A C AGC A AGGA A T T T T T GGGA A A A C C C A AG T AGGGG T T GGAG T A C AG
T C A T GC A A AG AGGG T T A T T C GGGA A A A C T C AGG T T GGAG T AGGGA T A C A C
T T A AGC A A A A AGGGA T T C T T GGA T A T T C C C AGA T CGGAGC CGGAG T T T A C

C A AGA AGGCG TGT T CCACAC A A T G T GGC A C G T C A C C AGGG GAGC T G T C C T

A A AGA AGGAG T C T T CCACAC C A T G T GGC A C G T C A C A AGAG GGGC AG T G T T
A T GGA AGG T G T A T T T CACAC A A T G T GGC A T G T A A C A AGAG GA T C AG T GA T
A A AGA AGGA A CA T T CCA T AC A A T G T GGC A T G T C A C A CG T G GCGC T G T T C T

CA TGT ACCAA GGGA AGAGA C T GGA A C C A AG T T GGGC C AG T G T C A A A A A AG

GA C A C A T A A T GGGA A A AGA C T GGA A C C A A A C T GGGC T AG T G T GA A A A A AG
C T GC C A CGAG A C T GGGAGA T T GGAGC C A T C T T GGGC T GA C G T C AGGA A T G
A A T GC A T A A A GGA A AGAGGA T T GA A C C A T C A T GGGCGGA C G T C A AGA A AG

A C T T GA T C T C A T A T GGAGGA GG T T GGAGG T T T C A AGGA T C C T GGA A CGCG

A T C T GA T T T C A T A T GGAGGA GGA T GGAGA C T GAGCGC A C A A T GGC A A A AG
A C A T GA T A T C A T A CGG T GGG GGA T GGAGGC T T GGAGA C A A A T GGGA C A A A
ACC T AA T A T C A T A T GGAGGA GGC T GGA AG T T AGA AGGAGA A T GGA AGGA A

GGAGA AGA AG T GC AGG T GA T T GC T G T T GA A C CGGGGA AGA ACCCCAAAAA

GGGGAGGAGG T GC AGG T T A T T GC CG T AGAG C C AGGGA AGA A C C C A A AGA A
GA AGA AGA CG T T C AGG T C C T CGC C A T AGA A C C AGGA A A A A A T CC T AAACA
GGAGA AGA AG T C C AGG T A T T GGC A C T GGAG C C T GGA A A A A A T C C A AGAGC

T G T A C AGA C A GCGC CGGG T A C C T T C A AGA C C C C T GA AGGC GA AG T T GGAG

C T T T CAAACC A CGC C AGGC A C T T T C C AGA C T A C T A C AGGG GA A A T AGGAG
T G T C C A A A CG A A A C C T GGC C T T T T C A AGA C C C T A A C T GGA GA A A T T GGAG
CG T C C A A A CG A A A C C T GG T C T T T T CAAAAC C A A CGC CGGA A C A A T AGG T G

C C A T AGC T C T AGA C T T T A A A C C CGGC A C A T C T GGA T C T C C T A T CG T GA A C

C A A T T GC A C T GGA T T T C A AG C C T GGA A C T T C AGGA T C T C C T A T CA T AAA T
C AG T A A C A T T AGA T T T C A A A C C CGGA A CG T C T GG T T C T C C CA T CA T CAAC
C T G T A T C T C T GGA C T T T T C T C C T GGA A CG T C AGGA T C T C C A A T T A T CGA C

AGAGAGGGA A A A A T AG T AGG T C T T T A T GGA A A T GGAG T GG T GA C A A C A AG

AGAGAGGGA A AGG T AG T GGG A C T G T A T GGC A A T GGAG T GG T T A C A A AGA A
AGGA A AGGA A A AG T C A T CGG A C T C T A T GGA A A T GGAG T AG T T ACCAAA T C
A A A A A AGGA A A AG T T G T GGG T C T T T A T GG T A A T GG T G T T G T T A C A AGGAG

10
T GG T A C C T A C G T C AG T GC C A T AGC T C A AGC T A A AGC A T C A C A AGA AGGGC
T GG T GGC T A T G T C AGCGGA A T AGCGC A A A C A A A T GC AGA A C C AGA T GGA C
AGG T GA T T A C G T C AG T GC C A T A A CGC A AGC CGA - - - A AGA A T T GGAGAGC
T GGAGC A T A T G T GAG T GC T A T AGC C C AGA C T GA A A A A AGC A T T GA AGA - -

C T C T A C C AGA GA T T GAGGA C GAGG T G T T T A GGA A A AGA A A C T T AACAA T A

CGA C A C C AGA G T T GGA AGA A GAGA T G T T C A A A A AGCGA A A C C T GA C C A T A
C AGA T T A T GA AG T GGA T GAG GA C A T T T T T C GA A AGA A A AG A T T AAC T A T A
- C A A C C C AGA GA T CGA AGA T GA C A T T T T C C GA A AGAGA AG A C T GA C C A T C

A T GGA C C T A C A T C C AGGA T C GGGA A A A A C A AGA AGA T A C C T T C C AGC C A T

A T GGA T C T T C A T C C T GGG T C AGGA A AGA C A CGGA A A T A C C T T C C AGC T A T
A T GGA C T T A C A C C C CGGAGC T GGA A AGA C A A A A AGA A T T C T T CCA T CAA T
A T GGA C C T C C A C C C AGGAGC GGGA A AGA CG A AGAGA T A C C T T C CGGC C A T

AG T C CG T GAG GC C A T A A A A A GA A AGC T GCG C A CGC T AG T C T T AGC T C C C A

T G T C AGAGAG GC A A T C A AGA GA CG T T T A AG AACC T T AA T T T T GGC A C CGA
AG T GAGAGA A GC C T T A A A A A GGAGGC T A CG A A C T T T GA T T T T AGC T C C C A
AG T C AGAGA A GC T A T A A A A C GGGG T T T GAG AACA T T AA T C T T GGC C C C C A

C A AGAG T T G T CGC T T C T GA A A T GGC AGAGG CGC T C A AGGG A A T GC C A A T A

C A AGGG T GG T T GC AGC T GAG A T GGA AGA AG C A T T GA A AGG GC T C C C A A T A
CGAGAG T GG T GGCGGC CGAG A T GGA AGAGG C C C T A CG T GG A C T GC C A A T C
C T AGAG T T G T GGC AGC T GA A A T GGAGGA AG C C C T T AGAGG AC T T CCAA T A

AGG T A T C AGA C A A C AGC AG T GA AGAG T GA A C A C A CGGGA A AGGAGA T AG T

AGG T A C C A A A C A A C AGC A A C A A A A T C T GA A C A C A C AGGA A GAGAGA T T G T
CG T T A T C AGA C C C C AGC T G T GA A A T C AGA A C A C A C AGGA A GAGAGA T T G T
AGA T A C C AGA C C C C AGC C A T C AGAGC T GAG C A C A C CGGGC GGGAGA T T G T

T GA C C T T A T G T G T C A CGC C A C T T T CAC T A T GCG T C T C C T G T C T C C T G T GA

T GA T C T A A T G T GC C A CGC A A CG T T C A C A A T GCG T T T GC T G T C A C C AG T T A
AGA C C T C A T G T G T C A T GC A A CC T T CACAAC A AGA C T T T T G T CA T CAACCA
GGA C C T A A T G T G T C A T GC C A CA T T T ACCA T GAGGC T GC T A T C A C C AG T T A

GAG T T C C C A A T T A T AA T A TG A T T A T C A T GG A T GA AGC A C A T T T T A C CGA T

GGG T T C C A A A T T ACAAC T TG A T A A T A A T GG A T GAGGC C C A T T T C A C AGA C
GGG T T C C A A A T T ACAACC T T A T AG T GA T GG A T GA AGC A C A T T T C A C CGA T
GAG T GC C A A A C T ACAACC TG A T T A T C A T GG A CGA AGC C C A T T T C A C AGA C

C C AGC C AGC A T AGC AGC C AG AGGG T A T A T C T C A A C C CGAG T GGG T A T GGG

C C AGC C AG T A T AGCGGC T AG AGGG T A C A T A T C A A C T CG T G T T GGA A T GGG
C C T T C T AG T G T CGCGGC T AG AGGA T A C A T C T CGA C C AGGG T GGA A A T GGG
C C AGC A AG T A T AGC AGC T AG AGGA T A C A T C T C A A C T CGAG T GGAGA T GGG

T GA AGC AGC T GCGA T T T T C A T GA C AGC C A C T C C C C C CGGA T CGG T GGAGG

AGAGGC AGC C GC A A T C T T C A T GA C AGC A A C A C C C C C T GGA A C AGC T GA T G
AGAGGC AGC A GC C A T C T T C A T GA C CGC A A C C C C T C C CGGA GCGA C AGA T C
T GAGGC AGC T GGGA T T T T T A T GA C AGC C A C T C C C C CGGGA AGC AGAGA C C

11
CC T T T CCACA GAGC A A T GC A G T T A T C C A AG A T GAGGA A AG AGA C A T T C C T
CC T T T CC T CA GAGC A A CGC T C C A A T T C A AG A T GA AGA A AG GGA C A T A C C A
CC T T T CCCCA GAGC A A C AGC C C A A T AGA AG A C A T CGAGAG GGA A A T T C CG
CA T T T CC T CA GAGC A A T GC A C C A A T C A T AG A T GA AGA A AG AGA A A T C C C T

GA A AGA T C A T GGA A C T C AGG C T A T GA C T GG A T C A C T GA T T T C C C AGG T A A

GA A CGC T C A T GGA A T T C AGG C A A T GA A T GG A T T A C CGA C T T CGC T GGGA A
GA A AGG T C A T GGA A C A C AGG G T T CGA C T GG A T A A C AGA C T A C C A AGGGA A
GA A CG T T CG T GGA A T T C CGG A C A T GA A T GG G T C A CGGA T T T T A A AGGGA A

A A C AG T C T GG T T TGT T CCAA GC A T C A A A T C AGGA A A T GA C A T T GC C A A C T

A A CGG T G T GG T T TGT CCC T A GC A T T A A AGC CGGA A A T GA C A T AGC A A A C T
A A C T G T G T GG T T TGT T CCCA GC A T A A A AGC T GGA A A T GA C A T T GC A A A T T
GA C T G T T T GG T T CG T T C C A A G T A T A A A AGC AGGA A A T GA T A T AGC AGC T T

G T T T A AGA A A GA A T GGGA A A CGGG T GG T C C A A T T GAGC AG AAAAAC T T T T

GC T T GCGA A A A A A CGGGA A A A A AG T C A T T C A A C T T AG T AG GA AGA C T T T T
G T T T GAGA A A G T CGGGA A AG A A AG T T A T C C AG T T GAG T AG GA A A A C C T T T
GC C T GAGGA A A A A T GGA A AG A A AG T GA T A C A A C T C AG T AG GA AGA C C T T T

GA C A C T GAG T A C C AGA A A A C AAAAAA T AAC GA C T GGGA C T A TGT TGT CAC

GA C A C AGA A T A T C AGA AGA C T A A A C T GA A T GA T T GGGA C T T T G T GG T GA C
GA T A C AGAG T A T CCAAAAAC GA A A C T C A CG GA C T GGGA C T T T G T GG T C A C
GA T T C T GAG T A T G T C A AGA C T AGA A C C A A T GA T T GGGA C T T CG T GG T T A C

A A C AGA C A T A T C CGA A A T GG GAGC A A A C T T C CGAGC CGA C AGGG T A A T AG

A A C T GA C A T T T C AGA A A T GG GGGC C A A T T T C A A AGC AGA T AGAG T GA T CG
T A C AGA C A T A T C T GA A A T GG GGGC C A A T T T T AGAGC CGGG AGAG T GA T AG
A A C T GA C A T T T C AGA A A T GG G T GC C A A T T T C A AGGC T GAG AGGG T T A T AG

A C C CGAGGCG G T GC C T GA A A C CGG T A A T A C T A A A AGA T GG C C C AGAGCG T

A C C C A AGA AG A TGT C T CAAA C C AG T GA T C T T GA C AGA T GG A C C AGAGCGG
A C C C T AGA AG A T GC C T C A AG C C AG T T A T C C T A C C AGA T GG GC C AGAGAGA
A C C C C AGA CG C T GC A T GA A A C C AG T C A T A C T A A C AGA T GG T GA AGAGCGG

G T C A T T C T AG C CGGA C CGA T GC C AG T GA C T G T GGC T AGCG C CGC C C AGAG

G T GA T C C T GG C CGGA C C A A T GC C AG T C A C C GCGGCGAG T G C T GCGC A A AG
G T C A T T T T AG C AGG T C C T A T T C C AG T GA C T C C AGC A AGCG C T GC T C AGAG
G T GA T T C T GG C AGGA C C T A T GC C AG T GA C C C A C T C T AG T G C AGC A C A A AG

GAGAGGA AGA A T T GGA AGGA ACCAAAA T AA GGA AGGCGA T C AG T A T A T T T

GAGAGGGAGA G T T GGC AGGA ACCCACAAAA AGAGA A T GA C C AG T A C A T A T
A AGAGGGCGA A T AGGA AGGA A C C C AGC A C A AGA AGA CGA C C A A T A CG T T T
A AGAGGGAGA A T AGGA AGA A A T CCAAAAAA T GAGA A T GA C C AG T A C A T A T

A C A T GGGA C A GC C T C T A A A C A A T GA T GAGG A C C A CGC C C A T T GGA C AGA A

T C A CGGGC C A GC C T C T C A A C A A T GA T GA AG A C C A T GC T C A C T GGA C AGA A
T C T C CGGAGA CCCAC T AAAA A A T GA T GA AG A T C A T GC C C A C T GGA C AGA A
A C A T GGGGGA A C C T C T GGA A A A T GA T GA AG A C T G T GC A C A C T GGA A AGA A

12
GC A A A A A T GC T C C T T GA C A A CA T AAACACA C C AGA AGGGA T T A T C C C AGC
GC A A A A A T GC T GC T GGA C A A CA T CAACACA C C AGA AGGGA T T A T A C C AGC
GC A A AGA T GC T GC T T GA C A A T A T C T ACACC C C AGA AGGGA T CA T T CCAAC
GC T A A A A T GC T C C T AGA T A A C A T C A A C A CG C C AGA AGGA A T C A T T C C T AG

C C T C T T T GAG C CGGAGAGAG A A A AGAG T GC AGC A A T AGA C GGGGA A T A C A

T C T C T T T GA A C C AGA A AGGG AGA AG T C AGC CGC C A T AGA C GG T GAG T A T C
A T T G T T T GG T C CGGA A AGGG AAAAAACCCA AGC C A T T GA T GGAGAG T T T C
C A T G T T CGA A C C AGAGCG T G A A A AGG T GGA T GC C A T T GA T GGCGA A T A C C

GA C T A CGGGG T GA AGCGAGG A A A A CG T T CG T GGAGC T C A T GAGA AGAGGA

GC C T GA AGGG T GAG T C C AGG A AGA C T T T CG T GGA A C T C A T GAGGAGGGG T
GC C T C AGAGG GGA A C A A AGG A AGA C T T T T G T GGA A T T A A T GAGGAGAGGA
GC T T GAGAGG AGA AGC A AGG AAAACC T T TG T AGA C T T A A T GAGA AGAGGA

GA T C T A C C T G T C T GGC T A T C C T A C A A AG T T GC C T C AGA AG GC T T C C AG T A
GA C C T T C C AG T T T GG T T AGC C C A T A A AG T A GC A T C AGA AG GA A T C A A A T A
GA C C T T C CGG T G T GGC T GAG C T A T A AGG T A GC T T C T GC T G GC A T T T C T T A
GA C C T A C C AG T C T GG T T GGC C T A C AGAG T G GC AGC T GA AG GC A T C A A C T A

C T C CGA C AGA AGG T GG T GC T T T GA T GGGGA A AGGA A C A A C C AGG T G T T GG

C A C AGA T AGA A A A T GG T GC T T T GA T GGGC A A CGC A A T A A T C A A A T T T T AG
CGA AGA T CGG GA A T GG T GC T T C A C AGGGGA A AGA A A T A A C C A A A T T T T AG
CGC AGA C AGA AGG T GG T G T T T T GA T GGAG T C A AGA A C A A C C A A A T C C T AG

AGGAGA A C A T GGA CG T GGAG A T C T GGA C A A A AGA AGGAGA A AGA A AGA A A

AGGAGA A C A T GGA T G T GGA A A T T T GGA C A A AGGA AGGAGA A A AGA A A A A A
A AGA A A A C A T GGAGG T T GA A A T T T GGA C T A GAGAGGGAGA A A AGA A A A AG
A AGA A A A CG T GGA AG T T GA A A T C T GGA C A A A AGA AGGGGA A AGGA AGA A A

C T A CGA C C C C GC T GGC T GGA T GC C AGA A C A T A C T C T GA C C C A C T GGC T C T

T T GAGA C C T A GG T GGC T T GA T GC C CGC A C T T A T T C AGA T C C A T T GGC A C T
C T A AGGC C A A GA T GG T T AGA T GC A CG T G T A T A CGC T GA C C C C A T GGC T T T
T T GA A A C C C A GA T GG T T GGA T GC T AGGA T C T A T T C T GA C C C A C T GGCGC T

GCGCGA A T T C A A AGAG T T CG C AGC AGGA AG A AGA AGCG T C T C AGG T GA C C

C A AGGA A T T C A AGGA C T T T G CGGC T GGC AG A A AG T C A A T C GC C C T T GA T C
GA AGGA T T T C A AGGAG T T T G C C AG T GGA AG GA AGAG T A T A A C T C T CGA C A
A A A AGA A T T T A AGGA A T T T G C AGC CGGA AG A A AG T C T C T G A C C C T GA A C C

T A A T A T T AGA A A T AGGGA A A C T T CCACAAC A T T T A A CGC A A AGGGC C C AG

T T G T GA C AGA A A T AGGA AGA G T GC C T T C A C A T C T AGC C C A C AGA A C A AGA
T C C T A A C AGA GA T T GC C AG T T T GC C A A C T T ACC T T T CC T C T AGGGC C A AG
T A A T C A C AGA A A T GGG T AGG C T CCCAACC T T C A T GA C T C A GA AGGC A AGA

A A CGC C T T GG A C A A T C T GG T T A T G T T GC A C A A C T C T GA A C A AGGAGGA A A
A A CGC T C T GG A C A A T C T GG T GA T GC T GC A T A CG T C AGA AG A T GGCGG T AG
C T CGC C C T T G A T A A C A T AG T C A T GC T C C A C A C A A C AGA A A GAGGAGGGAG
GA CGC A C T GG A C A A C T T AGC AG T GC T GC A C A CGGC T GAGG C AGG T GGA AG

13
AGC C T A T AGA C A CGC C A T GG A AGA A C T A C C AGA C A C C A T A GA A A CG T T A A
GGC T T A C AGG C A T GCGG T GG AGGA A C T A C C AGA A A C A A T G GA A A C A C T C C
GGC C T A T C A A C A CGC C C T GA A CGA A C T T C C GGAG T C A C T G GA A A C A C T C A
GGCG T A C A A C C A T GC T C T C A G T GA A C T GC C GGAGA C C C T G GAGA C A T T GC

T GC T C C T AGC T T T GA T AGC T G T GC T GA C T G G T GGAG T GA C GT TGT TCT TC

T A C T C T T GGG A C T A A T GA T C T T G T T GA C AG G T GGAGC A A T GC T T T T C T T G
T GC T T G T AGC T T T A C T AGG T GC T A T GA C AG C AGGC A T C T T CC TGT T T T T C
T T T T A C T GA C A C T T C T GGC T A C AG T C A CGG GAGGGA T C T T T T TAT TCT TG

C T A T C AGGA A GGGG T C T AGG AAAAACA T CC A T T GGC C T A C T C T GCG T GA T

A T A T C AGG T A A AGGGA T T GG A A AGA C T T C A A T AGGA C T C A T T TGTGT AA T
A T GC A AGGGA A AGGA A T AGG GA A A T T G T C A A T GGG T T T GA T A A C C A T T GC
A T GAGCGGA A GGGGC A T AGG GA AGA T GA C C C T GGGA A T G T GC T GC A T A A T

T GC C T C A AG T GC A C T G T T A T GGA T GGC C AG T G T GGA A C C C C A T T GGA T AG

CGC T T C C AGC GGC A T G T T G T GGA T GGC CGA AG T T C C A C T C C A A T GGA T CG
GG T GGC T AG T GGC T T GC T C T GGG T AGC AGA AA T T CAACCC C AG T GGA T AG
C A CGGC T AGC A T CC T CC T A T GG T A CGC A C A A A T A C AGC C A C A C T GGA T AG

CGGC C T C T A T C A T A C T GGAG T T C T T T C T GA T GG T G T T GC T T A T T C C AGAG

CG T CGGC T A T AG T C C T GGAG T T T T T T A T GA T GG T G T T GC T C A T A C C AGA A
CGGC C T C A A T C A T A C T AGAG T T T T T T C T CA T GG T A C T G T T GA T A C CGGA A
C AGC T T C A A T A A T A C T GGAG T T T T T T C T CA T AG T T T T GC T T A T T C C AGA A

C CGGA C AGA C AGCGC A C T C C A C A AGA C A A C C AGC T AGC A T A CG T GG T GA T

C C AGA A A AGC AGAGA A C C C C C C A AGA C A A C C A A C T CGC A T A T G T CG T GA T
C C AGA A A A A C A A AGGA C C C C A C A AGA C A A T C A A T T GA T C T A CG T C A T A T T
C C T GA A A A A C AGAGA A C A C C C C A AGA C A A C C A A C T GA C C T A CG T T G T C A T

AGG T C T G T T A T T C A T GA T A T T GA C AG T GGC AGC C A A T GAG A T GGGA T T A C

AGGC A T A C T T A C A T T GGC T G C A A C A A T AGC AGC C A A T GA A A T GGGA C T GC
GA C C A T T C T C ACCA T CA T TG G T C T A A T AGC AGC C A A CGAG A T GGGGC T GA
AGC C A T C C T C A C AG T GG T GG C CGC A A C C A T GGC A A A CGAG A T GGG T T T C C

T GGA A A C C A C A A AGA AGGA C C T GGGGA T T G G - - - - - T CA T G - - - C AGC T G

T GGA A A C C A C A A AGAGAGA C T T AGGA A T G T C - - - - - T A AG GAGC C AGG T G
T T GA A A A A A C A A A A A CGGA T T T T GGG T T T T A - - - - - C C AG G T A A A A A C AG
T AGA A A A A A C GA AGA A AGA T C T CGGA T T GG GA AGC A T T GC A A C C C AGC A A

C T GA A A A C C A C C A T C A T GC T GC A A T GC T GG A CG T AGA C C T A C A T C C AGC T
T TGT T - - TCT C C A A C C AGC T A T - - - - T T GG A T G T GGA C T T GC A C C C AGC A
- - - - AAACCA C C A T C - - - - - - - - - - - C T CG A T G T GGA C T T GAGA C C AGC T
C C CGAGAGC A A C A T C - - - - - - - - - - - C T GG A C A T AGA T C T A CG T C C T GC A

T C AGC C T GGA C T C T C T A T GC AG T GGC C A C A ACAA T T A T CA C T C C C A T GA T

T C AGC C T GGA C A T T G T A CGC CG T GGC C A C T A C AG T A A T A A CACCAA TGT T
T C AGC A T GGA CGC T C T A T GC AG T AGC C A C C A C A A T T C T GA C T C C C A T GC T
T C AGC A T GGA CGC T G T A T GC CG T GGC C A C A ACA T T TGT T A CACCAA TGT T

14
GAGA C A C A C A A T T GA A A A C A C A A CGGC A A A T A T T T CCC TG A C AGC T A T T G
A AGA C A T A C C A T AGAGA A T T C T A C AGC A A A TGTGT CCC TG GC AGC T A T AG
GAGA C A C A C C A T AGA A A A C A CG T CGGC C A A CC T A T C T C T A GC AGC C A T T G
GAGA C A T AGC A T T GA A A A T T C C T C AG T GA A TGTGT CCC T A A C AGC T A T AG

C A A A C C AGGC AGC T A T A T T G A T GGGA C T T G A C A AGGGA T G GC C A A T A T C A

C C A A C C AGGC AG T GG T C C T G A T GGG T T T GG A C A A AGGA T G GC C A A T A T C A
C C A A C C AGGC AGC CG T C C T A A T GGGGC T T G GA A A AGGA T G GC CGC T C C A C
C C A A C C A AGC C A C AG T G T T A A T GGG T C T CG GGA A AGGA T G GC C A T T G T C A

A AGA T GGA C A T AGGAG T T C C A C T T C T CGC C T T GGGG T GC T A T T C T C AGG T

A A A A T GGA C T T AGGCG T GC C A C T A C T GGC A C T GGG T T GC T A T T C A C A AG T
AGA A T GGA C C T CGG T G T GC C GC T G T T AGC A A T GGGA T GC T A T T C T C A AG T
A AGA T GGA C A T CGGAG T T C C C C T T C T CGC C A T T GGA T GC T A C T C A C A AG T

GA A C C CGC T G A CGC T GA C AG CGGCGG T A T T GA T GC T AG T G GC T C A T T A T G

GA A C C C A C T G AC T C T AAC TG CGGC AG T A C T T T T GC T A A T C ACACA T T A TG
GA A C C C A A C A A C C T T GA C AG C A T C C T T AG T C A T GC T T T T A GT CCA T T A TG
CAACCCCA T A A C T C T C A C AG C AGC T C T T T T C T T A T T GG T A GC A C A T T A T G

C C A T A A T T GG A C C CGGA C T G C A AGC A A A AG C T A C T AGAGA AGC T C A A A A A

C T A T C A T AGG T C C AGGA T T G C A AGC A A A AG C C A C C CG T GA AGC T C AGA A A
C A A T A A T AGG C C C AGGA T T G C AGGC A A A AG C C A C A AGAGA GGC C C AGA A A
C C A T C A T AGG GC C AGGA C T C C A AGC A A A AG C A A C C AGAGA AGC T C AGA A A

AGGA C AGC AG C CGGA A T A A T GA A A A A C C C A A C T G T CGA CG GGA T CG T T GC

AGGA C AGC T G C T GGA A T A A T GA AGA A T C C A A C AG T GGA T G GGA T A A T GA C
AGGA C AGC T G C T GGGA T C A T GA A A A A T C C C A C AG T GGA CG GGA T A A C AG T
AGAGC AGCGG CGGGC A T C A T GA A A A A C C C A A C T G T CGA T G GA A T A A C AG T

A A T AGA T T T G GA C C C T G T GG T T T A CGA T GC A A A A T T T GA A A A A C AGC T AG

A A T AGA C C T A GA T T C T G T A A T A T T T GA T T C A A A A T T T GA A A A A C A A C T GG
A A T AGA T C T A GA A C C A A T A T C C T A T GA C C C A A A A T T T GA A A AGC A A T T AG
GA T T GA C C T A GA T C C A A T A C C T T A T GA T C C A A AG T T T GA A A AGC AG T T GG

GC C A A A T A A T G T T G T T GA T A C T T T GC A C A T C A C AGA T C C T C C T GA T GCGG
GA C AGG T T A T GC T C C T GG T T T T G T GCGC AG T C C A A C T C T T GC T A A T GAGA
GGC AGG T C A T GC T A C T AG T C T T G T G T GC T G GA C A A C T A C T C T T GA T GAGA
GA C A AG T A A T GC T C C T AG T C C T C T GCG T GA C T C A AG T A T T GA T GA T GAGG

A C C A C A T GGG C C T T G T G T GA A T CCA T CACA C T AGC C A C T G GA C C T C T GA C

A C A T C A T GGG C C T T G T G T GA AGC T T T A A C T C T AGC T A C AG GA C C A A T A A C
A C A A C A T GGG C T T T C T G T GA AG T C T T GA C T T T GGC C A C AG GA C C A A T C T T
A C T A C A T GGG C T C T G T G T GA GGC T T T A A C C T T AGC T A C CG GGC C C A T C T C

T A CGC T T T GG GAGGGA T C T C C AGGA A A A T T C T GGA A C A C C A CGA T AGCGG

A A C A C T C T GG GA AGGA T C A C C T GG T A AG T T C T GGA A C A C C A CGA T AGC T G
GA C C T T G T GG GAGGGC A A C C CGGGA AGG T T T T GGA A C A CG A C C A T AGC CG
C A C A T T G T GG GA AGGA A A T C C AGGGAGG T T T T GGA A C A C T A C C A T T GCGG

15
T G T C C A T GGC AAACA T T T T T AGGGGA AG T T A T C T AGC AGG AGC AGG T C T G
T T T C C A T GGC GA A C A T T T T T AGAGGGAGC T A T T T AGC AGG AGC T GGGC T T
T A T C C A C CGC CAACA T T T T C AGGGGA AG T T A C T T GGCGGG AGC T GGA C T G
T G T C A A T GGC T AACA T T T T T AGAGGGAG T T A C T T GGC CGG AGC T GGA C T T

GC C T T T T C A T T A A T GA A A T C T C T AGGAGGA GG T AGGAGAG GC A CGGGAGC

GC T T T T T C T A T T A T GA A A T C AG T T GGA A C A GGA A A A AGAG GA A C AGGC T C
GC T T T T T C A C T C A T A A AGA A T GC A C A A A C C C C T AGGAGGG GA A C T GGGA C
CTCT T T TCTA T T A T GA AGA A CACAACCAAC A C A AGA AGGG GA A C T GGC A A

C C A AGGGGA A A C A C T GGGAG A A A A A T GGA A A AGA C AGC T A A A C C A A T T GA

A C A AGG T GA A A C T T T AGGAG A A A A A T GGA A A A AGA A A T T A AA T CAA T T A T
C A C AGGAGAG A C A C T GGGAG AGA AG T GGA A GAGA C AGC T A A A C T C A T T AG
C A T AGGAGAG A CGC T T GGAG AGA A A T GGA A A AGC CGA T T G A A CGC A T T GG

GC A AG T C AGA A T T CAACAC T T A C A A A AGGA G T GGGA T T A T AGAGG T GGA T

C C CGGA A AGA G T T T GA C C T T T A C A AGA A A T C T GGA A T C A C T GA AG T GGA T
A C AGA A A AGA G T T T GA AGAG T A T A A A AGA A G T GGA A T A C T AGA AG T GGA C
GA A A A AG T GA A T T C C AGA T C T A C A AGA A A A G T GGA A T C C A GGA AG T GGA T

AGA T C T GA AG C C A A AGAGGG G T T A A A A AGA GGAGA A A CGA C T A A A C A CGC

AGA A C AGA AG C C A A AGA AGG G T T GA A A AGA GGAGAGA C A A C A C A T C A T GC
AGGA C T GA AG C C A AG T C T GC C C T GA A AGA T GGG T C T A A A A T C A AGC A T GC
AGA A C C T T AG C A A A AGA AGG C A T T A A A AGA GGAGA A A CGG A C C A T C A CGC

AG T G T CGAGA GGA A CGGC C A A A C T GAGG T G G T T T G T GGAG AGGA A C C T T G

CG T G T C C CGA GG T AGCGC A A AAC T T CAA TG G T T T G T GGA A AGA A A C A T GG
AG T A T C A AGA GGG T C C AG T A AGA T C AGA T G GA T T G T T GAG AGAGGGA T GG
T G T G T CGCGA GGC T C AGC A A A A C T GAGA T G G T T CG T T GAG AGA A A C A T GG

T GA A A C C AGA AGGGA A AG T C A T AGA C C T CG G T T G T GGA AG AGG T GGC T GG

T CG T T C C CGA AGGA AGAG T C A T AGA C T T GG GC T G T GGA AG AGGAGGC T GG
T A A AGC C A A A AGGGA A AG T T G T AGA T C T T G GC T G T GGGAG AGGAGGA T GG
T C A C A C C AGA AGGGA A AG T A G T GGA C C T CG G T T G T GGC AG AGGAGGC T GG

T CA T A T T A T T GCGC T GGGC T GA AGA A AG T C A C AGA AG T GA A AGGA T A C A C

T CA T A T T AC T G T GC AGGA C T GA A A A A AG T C A C AGA AG T GC GAGGA T A C A C
T C T T A T T ACA T GGCGA C A C T C A AGA A CG T G A C T GA AG T GA A AGGG T A T A C
T CA T AC T A T T G T GGAGGA C T A A AGA A T G T A AGAGA AG T C A A AGGC C T A A C

GA A AGGAGGA C C T GGA C A T G AGGA A C C A A T C C C A A T GGC A A C C T A T GGA T

A A A AGGCGG T C C AGGA C A CG A AGA A C C AG T ACC T A TGT C T A C A T A T GGA T
A A A AGGAGG T C C AGGA C A T G A AGA A C CGA T T C C C A T GGC T A C T T A T GG T T
A A A AGGAGGA C C AGGA C A CG A AGA A C C C A T CCCCA TGT CA A C A T A T GGG T

GGA A C C T AG T A A AGC T A T A C T C CGGGA A AG ATGTAT TCT T T ACACCACC T

GGA A C A T AG T T A AG T T A A T G AGCGGA A AGG ATGTGT TCTA T C T CCCACC T
GGA A T T T GG T C A A A C T C C A T T C AGGGG T T G A CG T G T T C T A CAAACCCACA
GGA A T C T AG T GCG T C T T C A A AG T GGAG T T G A CG T T T T C T T C A T C C CGC C A

16
GAGA A A T G T G A C A C C C T C T T G T G T GA T A T T GG T GAG T C C T C T C CGA A C C C
GA A A AG T G T G A T A C C C T G T T G T G T GA C A T T GGAGA A T C T T C A C C A AGC C C
GAGC A AG T GG A C A C C C T GC T C T G T GA T A T T GGGGAG T C A T C T T C T AA T CC
GA A A AG T G T G A C A C A T T A T T G T G T GA C A T A GGGGAG T C A T CACCAAA T CC

A A C T A T AGA A GA AGGA AGA A CG T T A CG T G T T C T A A AGA T G G T GGA A C C A T

A A C AG T GGA A GAGAGC AGA A C T A T A AGAG T T T T GA AGA T G G T T GA A C C A T
A A C A A T AGAG GA AGGA AGA A C A T T A AGAG T T T T GA AGA T G G T GGAGC C A T
C A C AG T GGA A GC AGGA CGA A C A C T C AGAG T CC T T AAC T T A G T AGA A A A T T

GGC T C AGAGG AAACC - - - AA T T T T GC A T A A AAA T T C T AAA T CCC T A T A TG

GGC T A A A A A A C A A C C - - - AG T T T T GC A T T A A AG T T T T GA A CCC T T ACA TG
GGC T C T C T T C A A A A C C T GA A T T C T GC A T C A A AG T C C T T A A CCCC T ACA TG
GG T T GA A C A A CAACAC T CAA T T T T GC A T A A AGG T T C T C A A CCCA T A T A TG

C CGAG T G T GG T AGA A A C T T T GGAGC A A A T G C A A AGA A A A C A T GGAGGA A T

C C A A C T G T GA T T GAGC A C C T AGA A AGA C T A C A A AGGA A A C A T GGAGGA A T
C C A A C AG T C A T AGA AGAGC T GGAGA A A C T G C AGAGA A A A C A T GG T GGGA A
C C C T C AG T C A T AGA A A A A A T GGA AGC A C T A C A A AGGA A A T A T GGAGGAGC

GC T AG T GCGA AA T CCAC T C T C A AGA A A C T C C A C T C A T GA A A T G T A C T GGG

GC T T G T GAGA AA T CCAC T T T C A CGA A A C T C C A CGC A CGA A A T G T A C T GGA
C C T T G T C AGA T GC C CGC T G T C C AGGA A C T C C A C C C A T GAG A T G T A T T GGG
C T T AG T GAGG AA T CCAC T C T C A CGA A A C T C C A C A C A T GAG A T G T A C T GGG

T T T C A T G T GG A A C AGGA A A C A T T G T G T C AG C AG T A A A C A T GA C A T C T AGA
T A T C T A A T GG C A C AGG T A A C AT TGTCTCT T C AG T C A A C A T GG T G T C T AGA
T G T C AGGAGC G T CGGGA A A C A T T G T GAGC T C T G T GA A C A C A A C A T C A A AG
T A T C C A A T GC T T C CGGGA A C A T AG T G T C A T C AG T GA A C A T GA T T T C A AGG

A T GC T GC T A A A T CGA T T C A C A A T GGC T C A C AGGA AGC C A A C A T A T GA A AG

T T GC T A C T GA A C AGG T T C A C GA T GA C A C A C AGGAGA C C C A C C A T AGAGA A
A T G T T G T T GA A C AGG T T C A C A A C A AGGC A T AGGA A A C C C A C T T A T GAGA A
A T G T T GA T C A A C AGA T T T A C A A T GAGA T A C A AGA A AGC C A C T T A CGAGC C

AGA CG T GGA C T T AGGCGC T G GA A C A AGA C A T G T GGC AG T A GA A C C AGAGG

AGA T G T GGA T T T AGGAGC AG GA A C T CGA C A T G T T A A T GCG GA A C C AGA A A
GGA CG T AGA T C T T GGGGC AG GA A CGAGA AG TGT C T CCAC T GA A A C AGA A A
GGA T G T T GA C C T CGGA AGCG GA A C C CG T A A C A T CGGGA T T GA A AG T GAGA

T GGC C A A C C T AGA T A T C A T T GGC C AGAGGA T AGAGA A T A T A A A A A A T GA A

C A C C C A A C A T GGA T G T C A T T GGGGA A AGA A T A A A A AGGA T C A AGGAGGAG
A A C C AGA C A T GA C A A T C A T T GGGAGA AGGC T T C AGCGA T T GC A AGA AGAG
T A C C A A A C C T AGA T A T A A T T GGGA A A AGA A T AGA A A A A A T A A AGC A AGAG

CACAAA T CAA C A T GGC A T T A T GA T GAGGA C AA T CCA T ACA A A A C A T GGGC

CA T AA T T CAA C A T GGC A C T A T GA T GA CGA A AACCCC T ACA A A A CG T GGGC
C A C A A AGA A A C C T GGC A T T A T GA T C AGGA A AACCCA T ACA GA A C C T GGGC
C A T GA A A C A T C A T GGC A C T A T GA C C A AGA C CACCCA T ACA A A A CG T GGGC

17
C T A T C A T GGA T C A T A T GAGG T C A AGC C A T C AGGA T C AGC C T C A T C C A T GG
T T A C C A T GGA T C C T A T GA AG T C A A AGC C A C AGGC T C AGC C T C C T C C A T GA
G T A T C A T GGA AGC T A T GA AG C T C C T T CGA C AGGC T C T GC A T C C T C C A T GG
A T A C C A T GG T AGC T A T GA A A C A A A A C AGA C T GGA T C AGC A T C A T C C A T GG

T C A A T GG T G T GG T GAGA C T G C T AACCAAAC C A T GGGA T G T C A T T C C C A T G

T A A A T GGAG T CG T GA A A C T C C T CACCAAAC C A T GGGA T G T GG T GC C C A T G
T GA A CGGGG T GG T A A A A C T G C T AACAAAAC C C T GGGA T G T GA T T C C A A T G
T C A A CGGAG T GG T C AGGC T G C T GA C A A A A C C T T GGGA CG T CG T C C C C A T G

GT CACACAAA T AGC C A T GA C T GA C A C C A C A C C C T T T GGA C A A C AGAGGG T

G T GA C A C AGA T GGC A A T GA C AGA C A C A A C T C C A T T T GGC C AGC AGAGAG T
G T GA C T C AG T T AGC C A T GA C AGA T A C A A C C C C T T T T GGGC A A C A A AGAG T
G T GA C A C AGA T GGC A A T GA C AGA C A CGA C T C C A T T T GGA C A A C AGCGCG T

G T T T A A AGAG A A AG T T GA C A CGCG T A C A C C A A A AGCGA A A CGAGGC A C AG

C T T T A A AGAG A A AG T GGA C A C C AGGA CGC C C AGGC C C A T G C C AGGGA C A A
G T T C A A AGAG A AGG T GGA T A C C AGA A C A C C ACAACCAAAA C C CGG T A C A C
T T T T A A AGAG A A AG T GGA C A CGAGA A C C C A AGA A C CGA A A GA AGGC A CGA

CACAAA T T A T GGAGG T GA C A GC C AGG T GG T T A T GGGG T T T T C T C T C T AGA

GA A AGGC T A T GGAGA T C A C A GCGGAG T GGC T C T GGAGA A C C C T GGGA AGG
GA A T GG T T A T GA C C A CGA C A GC C A A T T GGC T G T GGGC C C T C C T T GGA A AG
AGA A A C T A A T GA A A A T A A C A GC AGAG T GGC T T T GGA A AGA A T T AGGGA AG

AACAAAAAAC C C AGA A T C T G C A C A AGAGAG GAG T T C A C A A GA A A AG T C AG

A A C A A A AGA C C C AGA T T A T G C A C A AGGGA A GAG T T T A C A A A A A AGG T C AG
A AGA A A A A T C C C AGA C T G T G C A C A AGGGA A GAG T T C A T C T C A A A AG T T AG
A A A A AGA C A C C C AGGA T G T G C A C C AGAGA A GA A T T C A C A A GA A AGG T GAG

G T C A A A CGC A GC T A T T GGAG C AG T G T T CG T T GA T GA A A A T C A A T GGA A C T

A A C T A A CGC A GC C A T GGGCG C CG T T T T C A C AGAGGAGA A C C A A T GGGA C A
A T C A A A CGC A GC C A T AGGCG C AG T C T T T C A GGA AGA A C AG GGA T GGA C A T
A AGC A A T GC A GC C T T GGGGG CCA T A T T CAC T GA T GAGA A C A AG T GGA AG T

C AGC A A A AGA GGC AG T GGA A GA T GA A CGG T T C T GGGA C C T T G T GC A C AGA

G T GCGA A AGC T GC T G T T GAG GA T GA AGA A T T T T GGA A A C T T G T GGA C AGA
C AGC C AG T GA AGC T G T GA A T GA C AGC CGG T T T T GGGA A C T GG T T GA C A A A
CGGC A CG T GA GGC T G T T GA A GA T AG T AGG T T T T GGGAGC T GG T T GA C A AG

GAGAGGGAGC T T CA T AAACA AGGA A A A T G T GC C A CG T G T G T C T ACAACA T

GA A CG T GA A C T CCACAAA T T GGGC A A A T G T GGA AGC T GCG T T T A T AACA T
GA A AGGGC C C T A C A C C AGGA AGGGA A A T G T GA A T CG T G T G T C T A T AACA T
GA A AGGA A T C T C C A T C T T GA AGGA A AG T G T GA A A C A T G T G TGT ACAACA T

GA T GGGA A AG AGAGAGA A A A A A T T AGGAGA G T T CGGA A AG GC A A A AGGA A

GA T GGGC A AG AGAGAGA A A A A A C T T GGAGA G T T T GGC A A A GC A A A AGGC A
GA T GGGA A A A CG T GAGA A A A AG T T AGGAGA G T T T GGC AGA GC C A AGGGA A
GA T GGGA A A A AGAGAGA AGA AGC T AGGGGA A T T CGGC A AG GC A A A AGGC A

18
G T CGCGC A A T A T GG T A C A T G T GG T T GGGAG CGCGC T T T T T AGAG T T T GA A
G T AGAGC T A T A T GG T A C A T G T GG T T GGGAG C C AGG T A C C T T GAG T T CGA A
GC CGAGC A A T C T GG T A C A T G T GGC T GGGAG CGCGG T T T C T GGA A T T T GA A
GC AGAGC C A T A T GG T A C A T G T GGC T T GGAG C A CGC T T C T T AGAG T T T GA A

GC C C T T GG T T T C A T GA A T GA AGA T C A C T GG T T C AGC AGAG AGA A T T C A C T

GC C C T T GGA T T C T T A A A T GA AGA C C A C T GG T T C T CGCG T G AAAAC T C T T A
GC C C T GGG T T T T T T GA A T GA AGA T C A C T GG T T T GGC AGAG AAAA T T CA TG
GC C C T AGGA T T C T T A A A T GA AGA T C A C T GG T T C T C C AGAG AGA A C T C C C T

C AG T GGAG T G GA AGGAGA AG GA C T C C A C A A A C T T GGA T A C A T A C T C AGAG

C AG T GGAG T A GA AGGAGA AG GA C T GC A C A A GC T AGGC T A C A T A T T A AGGG
GAG T GGAG T G GA AGGGGA AG G T C T GC A C AG A T T GGGA T A T A T C C T GGAGG
GAG T GGAG T G GA AGGAGA AG GGC T GC A C A A GC T AGG T T A C A T T C T A AGAG

ACA T A T CAAA GA T T C C AGGG GGA A A T A T G T A T GC AGA T GA C A C AGC CGGA

ACA T T T CCAA GA T A C C CGGA GGAGC C A T G T A T GC T GA T GA C A C AGC T GG T
AGA T AGA C A A GA AGGA T GGA GA C C T A A T G T A T GC T GA T GA C A C AGC AGGC
A CG T GAGC A A GA A AGAGGGA GGAGC A A T G T A T GC CGA T GA C A C CGC AGGA

T GGGA C A C A A GA A T A A C AGA GGA T GA T C T T C AGA A T GAGG CCAAAA T CAC

T GGGA C A C A A GA A T A A C AGA AGA T GA C C T G C A C A A T GAGG A A A AGA T C A T
T GGGA C A C A A GA A T C A C T GA GGA T GA C C T T C A A A A T GAGG A A C T GA T C A C
T GGGA T A C A A GA A T C A C A C T AGA AGA C C T A A A A A A T GA AG A A A T GG T A A C

T GA C A T C A T G GA A C C T GA A C A T GC C C T A T T GGC C A CG T C A A T C T T T A AGC
A C AGC A A A T G GA C C C T GA A C A C AGGC AG T T AGCGA A CGC T A T A T T C A AGC
GGA A C AGA T G GC T C C C C A C C A C A AGA T C C T AGC C A A AGC C A T T T T CAAAC
AAACCACA TG GA AGGAGA A C A C A AGA A A C T AGC CGAGGC C A T T T T CAAAC

T AACC T ACCA A A A C A AGG T A G T A AGGG T GC AGAGA C C AGC GA A A A A T GGA

T CACA T ACCA A A A C A A AG T G G T C A A AG T T C A A CGA C CGA C T C C A A CGGGC
T AACC T A T CA A A A C A A AG T G G T GA A AG T C C T C AGA C C C A C A C CGCGGGGA
T A A CG T A C C A A A A C A AGG T G G T GCG T G T GC A A AGA C C A A C A C C A AGAGGC

A C CG T GA T GG A TGT CA T A T C C AGA CG T GA C C AGAGAGGA A G T GGA C AGG T

A CGG T A A T GG ATAT TATATC T AGGA A AGA C C A A AGGGGC A G T GGA C A A C T
GCGG T GA T GG A T A T CA T A T C C AGGA A AGA C C A A AGAGG T A G T GGA C A AG T
A C AG T A A T GG ACA T CA T A T C GAGA AGAGA C C A A AGAGG T A G T GGA C A AG T

T GGA A C C T A T GGC T T A A A C A CC T T CACCAA C A T GGAGGC C CAAC T AA T AA

GGGA A C T T A T GGC C T GA A T A CA T T CACCAA C A T GGA AGC C C AG T T AG T C A
T GGA A C A T A T GG T T T GA A C A CA T T CACCAA C A T GGA AG T T CAAC T CA T CC
T GGC A C C T A T GGA C T C A A T A C T T T CACCAA T A T GGA AGC C CAAC T AA T CA

GA C A A A T GGA G T C T GAGGGA A T C T T T T C A C C C AGCGA A T T GGA A A C C C C A

GA C A A A T GGA AGGAGA AGG T G T GC T GA C A A AGGC AGA C C T CGAGA A C C C T
GC C A A A T GGA AGC T GA AGGA G T C A T C A C A C A AGA T GA C A T GC AGA A C C C A
GA C AGA T GGA GGGAGA AGGA G T C T T T A A A - - - - - - AGC A T T C AGC A C C T A

19
A A T C T - - - AG C CGA A AGAG T C C T CGA C - - - T GG T T GA A A A A A C A T GGC A C
C A T C T GC T AG AGA AGA A A A T CACACAA - - - T GG T T GGA A A C C A A AGGAG T
A A AGGG T T GA A AGA A AGAG T T GAGA A A - - - T GGC T GA A AG AG T G T GG T G T
A C A A T C A C AG A AGA A A T CGC T G T GC A A A A C T GG T T AGC A A GAG T GGGGCG

CGAGAGGC T G A A A AGA A T GG C A A T C AG T GG AGA T GA C T G T G T GG T GA A A C

GGAGAGG T T A A A A AGA A T GG C C A T T AGCGG GGA T GA T T GC G T GG T GA A A C
CGA C AGG T T A A AGAGGA T GG C A A T C AG T GG AGA CGA T T GC G T GG T GA AGC
CGA A AGG T T A T C A AGA A T GG C C A T C AG T GG AGA T GA T T G T G T T G T GA A A C

C A A T CGA T GA C AGA T T T GC A A C AGC C T T A A C AGC T T T GA A T GA C A T GGGA

C A A T CGA T GA C AGG T T CGC T A A T GC C C T GC T T GC T T T GA A CGA T A T GGGA
C C C T AGA T GA GAGG T T T GGC AC T T CCC T CC T C T T C T T GA A CGA C A T GGGA
C T T T AGA T GA C AGG T T CGC A AGCGC T T T A A C AGC T C T A A A T GA C A T GGGA

A AGG T A AGA A A AGA C A T A C C GC A A T GGGA A C C T T C A A A AG GA T GGA A T GA

A AGG T T CGGA A AGA C A T A C C T C A A T GGC AG C C A T C A A AGG GA T GGC A T GA
A AGG T GAGGA A AGA C A T T C C GC AG T GGGA A C C A T C T A AGG GA T GGA A A A A
A AGA T T AGGA A AGA C A T A C A A C A A T GGGA A C C T T C A AGAG GA T GGA A T GA

T T GGC A A C A A G T GC C T T T C T GT T CACACCA T T T C C A C C AG C T GA T T A T GA
T T GGC A A C AG GT T CC T T T C T GC T C C C A C C A C T T T C A T GA A T T GA T C A T GA
C T GGC A AGAG GT T CC T T T T T GC T C C C A C C A C T T T C A C A AG A T C T T T A T GA
T T GGA C A C A A G T GC C C T T C T GT T CACACCA T T T C C A T GAG T T A A T C A T GA

AGGA T GGGAG GGAGA T AG T G G T GC C A T GC C GC A A C C A AGA T GA A C T T G T A

A AGA T GGA AG A A AG T T GG T G G T T C C C T GC A GA C C C C AGGA CGA A C T A A T A
AGGA T GGC CG C T C A C T AG T T GT T CCA TGT A GA A A C C AGGA T GA A C T GA T A
A AGA CGG T CG CG T A C T CG T T GT T CCA TGT A GA A A C C A AGA T GA A C T GA T T

GG T AGGGC C A GAG T A T C A C A AGGCGC CGGA T GGAGC T T GA GAGA A A C T GC

GGA AGAGC A A GA A T C T C T C A AGGAGCGGGA T GGAGC C T T A GAGA A A C T GC
GGGAGAGC C A GA A T C T CGC A GGGAGC T GGA T GGAGC T T A A GAGA A A C AGC
GGC AGAGC C C GA A T C T C C C A AGGAGC AGGG T GG T C T T T GC GGGAGA CGGC

A T GC C T AGGC A AG T C A T A T G CACAAA TGTG GC AGC T GA T G T AC T T CCACA

A T G T C T GGGG A A AGC C T A CG CCCAAA TGTG GAG T C T C A T G T A T T T T CACA
C T GC C T GGGC A A AGC T T A CG C C C AGA T G T G G T CGC T T A T G T AC T T CCACA
C T G T T T GGGG A AG T C T T A CG CCCAAA TGTG GAGC T T GA T G T AC T T CCACA

GGAGAGA C T T GAGA T T AGCG GC T A A T GC T A T C T G T T C AGC CG T T C C AG T T

GA AGAGA T C T C AGA T T AGC A T C C A A CGC C A T A T G T T C AGC AG T A C C AG T C
GA AGGGA T C T GCG T T T AGC C T C C A T GGC C A T A T GC T C AGC AG T T C C A A CG
GA CGCGA C C T C AGGC T GGCG GC A A A T GC T A T T T GC T CGGC AG T A C C A T C A

GA T T GGG T C C C A A C C AGC CG C A C C A C C T GG T CGA T C C A T G CCCACCA T CA

C A C T GGG T T C C C A C A AG T AG A A CGA C A T GG T C T A T T CA TG C T CACCA T CA
GA A T GG T T T C C A A C A AGC AG A A C A A C A T GG T C A A T C C A CG C T CA T CACCA
C A T T GGG T T C C A A C A AG T CG A A C A A C C T GG T CCA T ACA TG C T A A A C A T GA

20
A T GGA T GA C A A C AGA AGA C A T G T T G T C AG T G T GGA A T AGG G T T T GGA T AG
G T GGA T GA C T A C AGA AGA C A T GC T T A C T G T T T GGA A C AGG G T G T GGA T AG
G T GGA T GA C C A C T GA AGA T A T GC T C A A AG T G T GGA A C AGA G T G T GGA T AG
A T GGA T GA C A A CGGA AGA C A T GC T GA C AG T C T GGA A C AGG G T G T GGA T T C

AGGA A A A C C C A T GGA T GGAG GA C A AGA C T C A T G T G T C C AG T T GGGA AGA C

AGGA A A A T C C A T GGA T GGA A GA C A A A A C T C C AG T T A C A A C T T GGGA A A A T
A AGA C A A C C C T A A T A T GA C T GA C A AGA C T C C AG T C C A T T C G T GGGA AGA T
A AGA A A A C C C A T GGA T GGA A GA C A A A A C T C C AG T GGA A T C A T GGGAGGA A

GT T CCA T ACC T AGGA A A A AG GGA AGA T C A A T GG T G T GG T T C C C T A A T AGG

GT T CCA T A T C T AGGA A AGAG AGA AGA C C A A T GG T G T GGA T C A C T T A T T GG
A T ACC T T ACC T AGGGA A A AG AGAGGA T T T G T GG T G T GGA T C C C T GA T T GG
A T CCCA T AC T T GGGGA A A AG AGA AGA C C A A T GG T GCGGC T C A T T GA T T GG

C T T A A C AGC A CGAGC C A C C T GGGC C A C C A A C A T A C A AG T G GC C A T A A A C C

T C T CAC T T CC AGAGC A A C C T GGGC C C AGA A CA T ACCCACA GC A A T T C A A C
AC T T T C T T CC AGAGC C A C C T GGGCGA AGA A C A T T C A T A CG GC C A T A A C C C
G T T A A C A AGC AGGGC C A C C T GGGC A A AGA A C A T C C A AGC A GC A A T A A A T C

A AG T GAGA AG GC T C A T T GGG A A T GAGA A T T A T C T AGA C T T C A T GA C A T C A

AGG T GAGA AG C C T T A T AGGC A A T GA AGAG T T C C T GGA C T A C A T GC C T T C A
AGG T C AGGA A C C T GA T CGGA A A AGAGGA A T A CG T GGA T T A C A T GC C AG T A
A AG T T AGA T C C C T T A T AGGC A A T GA AGA A T A C A C AGA T T A C A T GC C A T C C

A T GA AGAGA T T C A A A A A CGA GAG T GA T C C C GA AGGGGC A C T C T GG T A AGC

A T GA AGAGA T T C AGGA AGGA AGAGGAG T CG GAGGGAGC C A T T T GG T A A A C
A T GA A A AGA T A C AG T GC T C C T T C AGAGAG T GA AGGAG T T C TGT AA T T ACC
A T GA A A AGA T T C AGA AGAGA AGAGGA AGA A GC AGGAG T T C T G T GG T AGA -

C A A C T C A T T C A C A A A A T A A A GGA A A A T A A A A A A T C A A A C A AGGC A AGA AG

G T A - - - - - - - - - - - - - - - - - - GGA AG T GGA A A A - - - - - - G AGGC T A A C T G
- - - - - - - - - - - - - - - - - - - - - - - - AACAAC AAA - - - - - - - - - - - - - - - - -
- - - - - - - - - - - - - - - - - - - A AGC A A A A C T A A C A T GA A A C A AGGC T AGA AG

T C AGGC CGGA T T A AGC C A T A GC A CGG T A AG AGC T A T GC T G C C T G T GAGC C

T C AGGC C A C C T T A AGC C A C A G T A CGGA AGA AGC T G T GC T G C C T G T GAGC C
- - - - - - - - - - - - - - - - - - - - - - - CACCAAA GGC T A T - - - - - - - - - - - - - -
T C AGG T CGGA T T A AGC C A T A G T A CGGA A A A A A C T A T GC T A C C T G T GAGC C

C CG T C C A AGG A CG T - - - A A A A T GA AG T C AG GC - - - CG - A A A - GC C A CGG T
C CG T C C A AGG A CG T T A A A AG A AGA AG T C AG GC - - - C C C A A A - GC C A CGG T
- - - - - - - - - - - - - - - - - - - - - T GA AG T C AG GC - - - C A C T T G T GC C A CGG T
C CG T C C A AGG A CG T T A A A A - - - GA AG T C AG GC C A T C A T A A A T GC C A T AGC

T CGAGC A AGC CG T GC T GC C T G T AGC T C C A T CG T - - - - - GG GGA T G T A A A A

T T GAGC A A A C CG T GC T GC C T G T AGC T C CG T CG T - - - - - GG GGA CG T A A A A
T T GAGC A A A C CG T GC T GC C T G T AGC T C CGC C A A T A A T GGG AGGCG T A A T A
T T GAG T A A A C T A T GC AGC C T G T AGC T C C A C C T G - - - - AGA AGG T G T A A A A

21
A C C C - - - GGG AGGC T GC A A A C C A T GGA AGC T G T A CGC A T G GGG T AGC AGA
- C C T - - - GGG AGGC T GC A A A C T G T GGA AGC T G T A CGC A CG G T G T AGC AGA
A T C C C C AGGG AGGC C A T GCG C C A CGGA AGC T G T A CGCG T G GC A T A T T GGA
A A T C C - - GGG AGGC C A C A A A C C A T GGA AGC T G T A CGC A T G GCG T AG T GGA

C T AG T GG T T A GAGGAGA C C C C T C C C A A - - - - GA C A C A A CG C AGC AGC - - -

C T AGCGG T T A GAGGAGA C C C C T C C C A T - - - - GA C A C A A CG C AGC AGC - - -
C T AGCGG T T A GAGGAGA C C C C T C C C A T C A C T GA T A A A A CG C AGC A A A AG -
C T AGCGG T T A GAGGAGA C C C C T C C C T T - - - - - A C A A A T CG C AGC A A C A A T

GGGGC C C A A - C A C C AGGGGA AGC T G T A C C C T GG T GG T A AG GA C T AGAGG T

GGGGC C CGAG C A C T GAGGGA AGC T G T A C C T C C T T GC A A AG GA C T AGAGG T
GGGGC C CGA A GC C AGGAGGA AGC T G T A C T C C T GG T GGA AG GA C T AGAGG T
GGGGGC C C A A GGCGAGA T GA AGC T G T AG T C T CGC T GGA AG GA C T AGAGG T

T AGAGGAGA C C C C C CGC A C A ACAACAAACA GC A T A T T GA C GC T GGGAGAG

T AGAGGAGA C C C C C CGC A - A A T AA - AAACA GC A T A T T GA C GC T GGGAGAG
T AGAGGAGA C CCCCCCAACA CAAA - - AACA GC A T A T T GA C GC T GGGA A AG
T AGAGGAGA C C C C C C CGA - A ACAAAAAACA GC A T A T T GA C GC T GGGA A AG

A C C AGAGA T C C T GC T G T C T C T A C AGC A T C A T T C C AGGC A C AGA A CGC C A A

A C C AGAGA T C C T GC T G T C T C C T C AGC A T C A T T C C AGGC A C AGA A CGC C AG
A C C AGAGA T C C T GC T G T C T C T GC A A C A T C A A T C C AGGC A C AGAGCGC CGC
A C C AGAGA T C C T GC T G T C T C C T C AGC A T C A T T C C AGGC A C AGA A CGC C AG

A A A A T GGA A T GG T GC T G T T G A A T C A A C AGG T TCT

A A A A T GGA A T GG T GC T G T T G A A T C A A C AGG T TCT
A AGA T GGA T T GG T G T T G T T G A T C C A A C AGG T TCT
A A A A T GGA A T GG T GC T G T T G A A T C A A C AGG T TCT

22
 Phosphorothioate
Oligomer Panel
List of phosphorothioate oligomers designed
for all four serotypes of the Dengue Virus
Phosphorothioate Oligomer Panel

Dengue Virus Type 1

Site 1

Site 2

Site 3

Site 4

Site 5

Site 6

Site 7

Site 8

Site 9

Site 10

Site 11

Site 12

Site 13

Site 14

Site 15

Site 16

Site 17

Site 18

Site 19

Site 20

1 1000010735
ACC# U88536.1 1

5' C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A*T*C*C*T 3'

Length: 21 3' End Position: 9725

G + C%: 57.1 Tm: 42.9

AntiSense MW: 6494.3

ACC# U88536.1 2

5' C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A*T*C*C 3'

Length: 20 3' End Position: 9726

G + C%: 60 Tm: 42.3

AntiSense MW: 6174.0

ACC# U88536.1 3

5' G*C*A*C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A 3'

Length: 20 3' End Position: 9729

G + C%: 60 Tm: 42.9

AntiSense MW: 6223.0

ACC# U88536.1 4

5' A*C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A*T*C*C 3'

Length: 21 3' End Position: 9726

G + C%: 57.1 Tm: 42.0

AntiSense MW: 6503.3

ACC# U88536.1 5

5' G*C*A*C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A*T 3'

Length: 21 3' End Position: 9728

G + C%: 57.1 Tm: 42.1

AntiSense MW: 6543.3

ACC# U88536.1 6

5' C*A*C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A*T*C 3'

Length: 21 3' End Position: 9727

G + C%: 57.1 Tm: 40.2

AntiSense MW: 6503.3

ACC# U88536.1 7

5' C*A*C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A 3'

Length: 19 3' End Position: 9729

G + C%: 57.9 Tm: 39.4

AntiSense MW: 5877.8

ACC# U88536.1 8

5' T*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C*T 3'

Length: 21 3' End Position: 9082

G + C%: 52.4 Tm: 41.5

AntiSense MW: 6500.3

ACC# U88536.1 9

5' G*T*C*T*C*A*G*C*C*A*C*T*T*C*C*T*T*C*T*C*T 3'

Length: 21 3' End Position: 1857

G + C%: 52.4 Tm: 42.2

AntiSense MW: 6580.3

ACC# U88536.1 10

5' A*C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A*T*C 3'

Length: 20 3' End Position: 9727

G + C%: 55 Tm: 39.3

AntiSense MW: 6198.0

ACC# U88536.1 11

5' C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C*T 3'

Length: 20 3' End Position: 9082

G + C%: 55 Tm: 40.8

AntiSense MW: 6180.0

ACC# U88536.1 12

5' T*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C 3'

Length: 20 3' End Position: 9083

G + C%: 55 Tm: 40.7

AntiSense MW: 6180.0

ACC# U88536.1 13

5' A*C*A*C*C*C*T*C*C*T*C*C*C*A*T*G*C*C*T*T 3'

Length: 20 3' End Position: 2560

G + C%: 60 Tm: 43.9

AntiSense MW: 6214.0

ACC# U88536.1 14

5' G*T*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A 3'

Length: 20 3' End Position: 9084

G + C%: 55 Tm: 43.1

AntiSense MW: 6220.0

ACC# U88536.1 15

5' T*T*C*C*A*C*A*C*A*C*A*C*C*C*T*C*C*T*C*C 3'

Length: 20 3' End Position: 2568

G + C%: 60 Tm: 40.6

AntiSense MW: 6183.0

ACC# U88536.1 16

5' G*T*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C 3'

Length: 21 3' End Position: 9083

G + C%: 57.1 Tm: 43.5

AntiSense MW: 6525.3

ACC# U88536.1 17

5' C*A*C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A*T 3'

Length: 20 3' End Position: 9728

G + C%: 55 Tm: 38.8

AntiSense MW: 6198.0

ACC# U88536.1 18

5' C*C*A*C*T*A*T*C*T*C*C*C*T*C*C*C*A*T*C 3'

Length: 19 3' End Position: 9727

G + C%: 57.9 Tm: 39.5

AntiSense MW: 5868.8

ACC# U88536.1 19

5' T*G*C*C*T*C*T*C*C*T*A*C*C*T*C*C*T*C*C*T 3'

Length: 20 3' End Position: 7557

G + C%: 60 Tm: 45.9

AntiSense MW: 6196.0

ACC# U88536.1 20

5' G*C*C*T*C*T*C*C*T*A*C*C*T*C*C*T*C*C*T*A 3'

Length: 20 3' End Position: 7556

G + C%: 60 Tm: 45.7

AntiSense MW: 6205.0

Dengue Virus Type 2.

Site 1

Site 2

Site 3

Site 4

Site 5

Site 6

Site 7

Site 8

Site 9

Site 10

Site 11

Site 12

Site 13

Site 14

Site 15

Site 16

Site 17

Site 18

Site 19

Site 20

1 1000010723
Dengue 2 1

5' C*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C*T 3'

Length: 21 3' End Position: 9081

G + C%: 57.1 Tm: 43.4

AntiSense MW: 6485.3

Dengue 2 2

5' C*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C 3'

Length: 20 3' End Position: 9082

G + C%: 60 Tm: 42.7

AntiSense MW: 6165.0

Dengue 2 3

5' G*T*C*T*C*C*A*G*T*C*C*C*A*T*T*C*C*C*A*C 3'

Length: 20 3' End Position: 732

G + C%: 60 Tm: 44.1

AntiSense MW: 6254.1

Dengue 2 4

5' G*C*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A 3'

Length: 20 3' End Position: 9083

G + C%: 60 Tm: 45.7

AntiSense MW: 6205.0

Dengue 2 5

5' C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C*T*C 3'

Length: 21 3' End Position: 9080

G + C%: 57.1 Tm: 42.2

AntiSense MW: 6485.3

Dengue 2 6

5' G*T*C*T*C*C*A*G*T*C*C*C*A*T*T*C*C*C*A*C*A 3'

Length: 21 3' End Position: 731

G + C%: 57.1 Tm: 44.0

AntiSense MW: 6583.3

Dengue 2 7

5' C*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A 3'

Length: 19 3' End Position: 9083

G + C%: 57.9 Tm: 42.2

AntiSense MW: 5859.8

Dengue 2 8

5' C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C*T 3'

Length: 20 3' End Position: 9081

G + C%: 55 Tm: 40.8

AntiSense MW: 6180.0

Dengue 2 9

5' A*G*C*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C 3'

Length: 20 3' End Position: 9084

G + C%: 60 Tm: 45.2

AntiSense MW: 6205.0

Dengue 2 10

5' A*G*C*C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A 3'

Length: 21 3' End Position: 9083

G + C%: 57.1 Tm: 45.1

AntiSense MW: 6534.3

Dengue 2 11

5' T*C*T*C*C*A*G*T*C*C*C*A*T*T*C*C*C*A*C*A 3'

Length: 20 3' End Position: 731

G + C%: 55 Tm: 41.2

AntiSense MW: 6238.1

Dengue 2 12

5' C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C*T*C*A 3'

Length: 21 3' End Position: 9079

G + C%: 52.4 Tm: 39.6

AntiSense MW: 6509.3

Dengue 2 13

5' C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C*T*C 3'

Length: 20 3' End Position: 9080

G + C%: 55 Tm: 39.4

AntiSense MW: 6180.0

Dengue 2 14

5' C*C*T*T*C*T*C*C*T*T*C*C*A*C*T*C*C*A*C 3'

Length: 19 3' End Position: 9082

G + C%: 57.9 Tm: 39.9

AntiSense MW: 5859.8

Dengue 2 15

5' T*G*T*C*T*C*C*A*G*T*C*C*C*A*T*T*C*C*C*A*C 3'

Length: 21 3' End Position: 732

G + C%: 57.1 Tm: 43.0

AntiSense MW: 6574.3

Dengue 2 16

5' C*T*C*C*T*T*C*T*C*C*C*T*C*C*A*T*C*T*G*T*C 3'

Length: 21 3' End Position: 9430

G + C%: 57.1 Tm: 44.2

AntiSense MW: 6516.3

Dengue 2 17

5' A*C*T*C*C*T*T*C*T*C*C*C*T*C*C*A*T*C*T 3'

Length: 19 3' End Position: 9433

G + C%: 52.6 Tm: 40.5

AntiSense MW: 5874.8

Dengue 2 18

5' T*C*T*C*C*A*G*T*C*C*C*A*T*T*C*C*C*A*C 3'

Length: 19 3' End Position: 732

G + C%: 57.9 Tm: 41.2

AntiSense MW: 5908.8

Dengue 2 19

5' T*C*T*C*C*A*G*T*C*C*C*A*T*T*C*C*C*A*C*A*T 3'

Length: 21 3' End Position: 730

G + C%: 52.4 Tm: 40.5

AntiSense MW: 6558.3

Dengue 2 20

5' T*C*C*T*T*C*T*C*C*C*T*C*C*A*T*C*T*G*T*C*T 3'

Length: 21 3' End Position: 9429

G + C%: 52.4 Tm: 43.5

AntiSense MW: 6531.3

Dengue Virus Type 3

Site 1

Site 2

Site 3

Site 4

Site 5

Site 6

Site 7

Site 8

Site 9

Site 10

Site 11

Site 12

Site 13

Site 14

Site 15

Site 16

Site 17

Site 18

Site 19

Site 20

1 1000010707
ACC# AY099336.1 1

5' T*C*C*C*A*C*T*A*C*C*T*T*T*C*C*C*T*C*T*C*T 3'

Length: 21 3' End Position: 4936

G + C%: 52.4 Tm: 41.5

AntiSense MW: 6500.3

ACC# AY099336.1 2

5' C*C*C*A*C*T*A*C*C*T*T*T*C*C*C*T*C*T*C*T 3'

Length: 20 3' End Position: 4936

G + C%: 55 Tm: 40.8

AntiSense MW: 6180.0

ACC# AY099336.1 3

5' T*C*C*C*A*C*T*A*C*C*T*T*T*C*C*C*T*C*T*C 3'

Length: 20 3' End Position: 4937

G + C%: 55 Tm: 40.7

AntiSense MW: 6180.0

ACC# AY099336.1 4

5' G*T*C*C*C*A*C*T*A*C*C*T*T*T*C*C*C*T*C*T*C 3'

Length: 21 3' End Position: 4937

G + C%: 57.1 Tm: 43.5

AntiSense MW: 6525.3

ACC# AY099336.1 5

5' G*T*T*C*C*T*G*C*C*A*A*C*T*C*T*C*C*C*T*C*T 3'

Length: 21 3' End Position: 5887

G + C%: 57.1 Tm: 43.1

AntiSense MW: 6565.3

ACC# AY099336.1 6

5' G*T*C*C*C*A*C*T*A*C*C*T*T*T*C*C*C*T*C*T 3'

Length: 20 3' End Position: 4938

G + C%: 55 Tm: 42.1

AntiSense MW: 6220.0

ACC# AY099336.1 7

5' G*T*T*C*C*T*G*C*C*A*A*C*T*C*T*C*C*C*T*C 3'

Length: 20 3' End Position: 5888

G + C%: 60 Tm: 42.4

AntiSense MW: 6245.0

ACC# AY099336.1 8

5' C*T*G*C*C*A*A*C*T*C*T*C*C*C*T*C*T*C*C*T 3'

Length: 20 3' End Position: 5884

G + C%: 60 Tm: 42.4

AntiSense MW: 6205.0

ACC# AY099336.1 9

5' C*C*C*A*C*T*A*C*C*T*T*T*C*C*C*T*C*T*C 3'

Length: 19 3' End Position: 4937

G + C%: 57.9 Tm: 40.0

AntiSense MW: 5859.8

ACC# AY099336.1 10

5' T*C*C*T*G*C*C*A*A*C*T*C*T*C*C*C*T*C*T*C 3'

Length: 20 3' End Position: 5886

G + C%: 60 Tm: 42.3

AntiSense MW: 6205.0

ACC# AY099336.1 11

5' T*C*C*A*C*A*C*A*C*T*C*C*A*T*T*C*T*C*C*C*A 3'

Length: 21 3' End Position: 2560

G + C%: 52.4 Tm: 38.2

AntiSense MW: 6527.3

ACC# AY099336.1 12

5' G*C*C*A*A*C*T*C*T*C*C*C*T*C*T*C*C*T*T*T 3'

Length: 20 3' End Position: 5882

G + C%: 55 Tm: 40.9

AntiSense MW: 6220.0

ACC# AY099336.1 13

5' T*C*C*C*T*C*C*G*A*C*T*C*C*T*C*T*T*C*C*T 3'

Length: 20 3' End Position: 10235

G + C%: 60 Tm: 43.9

AntiSense MW: 6196.0

ACC# AY099336.1 14

5' C*T*G*C*C*A*A*C*T*C*T*C*C*C*T*C*T*C*C*T*T 3'

Length: 21 3' End Position: 5883

G + C%: 57.1 Tm: 41.9

AntiSense MW: 6525.3

ACC# AY099336.1 15

5' T*C*C*C*A*C*T*A*C*C*T*T*T*C*C*C*T*C*T 3'

Length: 19 3' End Position: 4938

G + C%: 52.6 Tm: 39.2

AntiSense MW: 5874.8

ACC# AY099336.1 16

5' T*C*C*A*C*A*C*A*C*T*C*C*A*T*T*C*T*C*C*C 3'

Length: 20 3' End Position: 2561

G + C%: 55 Tm: 38.0

AntiSense MW: 6198.0

ACC# AY099336.1 17

5' C*C*C*A*C*T*A*C*C*T*T*T*C*C*C*T*C*T*C*T*A 3'

Length: 21 3' End Position: 4935

G + C%: 52.4 Tm: 39.7

AntiSense MW: 6509.3

ACC# AY099336.1 18

5' T*T*C*C*T*G*C*C*A*A*C*T*C*T*C*C*C*T*C*T*C 3'

Length: 21 3' End Position: 5886

G + C%: 57.1 Tm: 41.8

AntiSense MW: 6525.3

ACC# AY099336.1 19

5' G*C*C*A*A*C*T*C*T*C*C*C*T*C*T*C*C*T*T 3'

Length: 19 3' End Position: 5883

G + C%: 57.9 Tm: 41.3

AntiSense MW: 5899.8

ACC# AY099336.1 20

5' T*T*C*C*A*C*A*C*A*C*T*C*C*A*T*T*C*T*C*C*C 3'

Length: 21 3' End Position: 2561

G + C%: 52.4 Tm: 37.7

AntiSense MW: 6518.3

Dengue Virus Type 4

Site 1

Site 2

Site 3

Site 4

Site 5

Site 6

Site 7

Site 8

Site 9

Site 10

Site 11

Site 12

Site 13

Site 14

Site 15

Site 16

Site 17

Site 18

Site 19

Site 20

1 1000010649
ACC# AF326825.1 1

5' A*T*C*C*T*C*C*T*C*T*C*C*C*A*C*A*G*C*C*A 3'

Length: 20 3' End Position: 7801

G + C%: 60 Tm: 44.6

AntiSense MW: 6223.0

ACC# AF326825.1 2

5' G*C*C*T*C*T*C*C*C*A*T*T*T*C*C*A*C*C*C*T 3'

Length: 20 3' End Position: 5429

G + C%: 60 Tm: 45.3

AntiSense MW: 6205.0

ACC# AF326825.1 3

5' T*C*C*T*C*C*T*C*T*C*C*C*A*C*A*G*C*C*A*A 3'

Length: 20 3' End Position: 7800

G + C%: 60 Tm: 42.5

AntiSense MW: 6223.0

ACC# AF326825.1 4

5' C*C*C*T*C*C*C*T*C*C*T*C*T*T*T*C*T*G*T*T 3'

Length: 20 3' End Position: 6475

G + C%: 55 Tm: 43.9

AntiSense MW: 6202.0

ACC# AF326825.1 5

5' C*C*T*C*T*C*C*C*A*T*T*T*C*C*A*C*C*C*T 3'

Length: 19 3' End Position: 5429

G + C%: 57.9 Tm: 42.0

AntiSense MW: 5859.8

ACC# AF326825.1 6

5' T*C*T*C*T*T*C*C*A*C*T*T*C*T*C*T*C*C*C*A 3'

Length: 20 3' End Position: 7587

G + C%: 50 Tm: 39.6

AntiSense MW: 6195.0

ACC# AF326825.1 7

5' C*C*C*T*C*C*C*T*C*C*T*C*T*T*T*C*T*G*T 3'

Length: 19 3' End Position: 6476

G + C%: 57.9 Tm: 44.5

AntiSense MW: 5881.8

ACC# AF326825.1 8

5' A*T*C*C*T*C*C*T*C*T*C*C*C*A*C*A*G*C*C*A*A 3'

Length: 21 3' End Position: 7800

G + C%: 57.1 Tm: 41.7

AntiSense MW: 6552.3

ACC# AF326825.1 9

5' G*C*C*C*T*C*C*C*T*C*C*T*C*T*T*T*C*T*G*T 3'

Length: 20 3' End Position: 6476

G + C%: 60 Tm: 48.0

AntiSense MW: 6227.0

ACC# AF326825.1 10

5' A*C*C*A*T*C*C*T*C*C*T*C*T*C*C*C*A*C*A 3'

Length: 19 3' End Position: 7805

G + C%: 57.9 Tm: 41.3

AntiSense MW: 5877.8

ACC# AF326825.1 11

5' C*C*C*T*C*C*C*T*C*C*T*C*T*T*T*C*T*G*T*T*G 3'

Length: 21 3' End Position: 6474

G + C%: 57.1 Tm: 43.6

AntiSense MW: 6547.3

ACC# AF326825.1 12

5' G*T*C*T*C*T*T*C*C*A*C*T*T*C*T*C*T*C*C*C 3'

Length: 20 3' End Position: 7588

G + C%: 55 Tm: 42.5

AntiSense MW: 6211.0

ACC# AF326825.1 13

5' T*G*C*C*T*C*T*C*C*C*A*T*T*T*C*C*A*C*C*C*T 3'

Length: 21 3' End Position: 5429

G + C%: 57.1 Tm: 44.2

AntiSense MW: 6525.3

ACC# AF326825.1 14

5' C*C*T*C*T*C*C*C*A*T*T*T*C*C*A*C*C*C*T*G 3'

Length: 20 3' End Position: 5428

G + C%: 60 Tm: 41.8

AntiSense MW: 6205.0

ACC# AF326825.1 15

5' C*C*T*C*C*C*T*C*C*T*C*T*T*T*C*T*G*T*T*G*T 3'

Length: 21 3' End Position: 6473

G + C%: 52.4 Tm: 43.1

AntiSense MW: 6562.3

ACC# AF326825.1 16

5' G*T*C*T*C*T*T*C*C*A*C*T*T*C*T*C*T*C*C*C*A 3'

Length: 21 3' End Position: 7587

G + C%: 52.4 Tm: 42.6

AntiSense MW: 6540.3

ACC# AF326825.1 17

5' T*C*T*C*T*T*C*C*A*C*T*T*C*T*C*T*C*C*C 3'

Length: 19 3' End Position: 7588

G + C%: 52.6 Tm: 39.4

AntiSense MW: 5865.8

ACC# AF326825.1 18

5' A*C*C*A*T*C*C*T*C*C*T*C*T*C*C*C*A*C*A*G 3'

Length: 20 3' End Position: 7804

G + C%: 60 Tm: 41.6

AntiSense MW: 6223.0

ACC# AF326825.1 19

5' G*C*C*C*T*C*C*C*T*C*C*T*C*T*T*T*C*T*G*T*T 3'

Length: 21 3' End Position: 6475

G + C%: 57.1 Tm: 47.2

AntiSense MW: 6547.3

ACC# AF326825.1 20

5' T*G*C*C*T*C*T*C*C*C*A*T*T*T*C*C*A*C*C*C 3'

Length: 20 3' End Position: 5430

G + C%: 60 Tm: 43.7

AntiSense MW: 6205.0

Dengue Virus Type 1

Set Overview

Site 1

Site 2

Site 3

Site 4

Site 5

Site 6

Site 7

Site 8

Site 9

Site 10

Site 11

Site 12

Site 13

Site 14

Site 15

Site 16

Site 17

Site 18

Site 19

Site 20

1 1000010735
Dengue 1 RNAi Silenceome
1

Sense Position: 1375 AntiSense Position: 1399

Sense Length: 25 AntiSense Length: 27

Sense MW: 7994.9 AntiSense MW: 8540.1

Total MW: 16535.0 Core GC%: 47.4

Asymmetrical End
5.56
Stability Difference:

Dengue 1 RNAi Silenceome

2

Sense Position: 2346 AntiSense Position: 2370

Sense Length: 25 AntiSense Length: 27

Sense MW: 7955.8 AntiSense MW: 8609.2

Total MW: 16565.0 Core GC%: 52.6

Asymmetrical End
4.62
Stability Difference:
Dengue 1 RNAi Silenceome
3

Sense Position: 17 AntiSense Position: 41

Sense Length: 25 AntiSense Length: 27

Sense MW: 8009.9 AntiSense MW: 8547.1

Total MW: 16557.1 Core GC%: 47.4

Asymmetrical End
4.93
Stability Difference:

Dengue 1 RNAi Silenceome

4

Sense Position: 2499 AntiSense Position: 2523

Sense Length: 25 AntiSense Length: 27

Sense MW: 7940.9 AntiSense MW: 8589.1

Total MW: 16530.0 Core GC%: 42.1

Asymmetrical End
6.02
Stability Difference:
Dengue 1 RNAi Silenceome
5

Sense Position: 7174 AntiSense Position: 7198

Sense Length: 25 AntiSense Length: 27

Sense MW: 7886.8 AntiSense MW: 8656.3

Total MW: 16543.0 Core GC%: 36.8

Asymmetrical End
6.82
Stability Difference:

Dengue 1 RNAi Silenceome

6

Sense Position: 6518 AntiSense Position: 6542

Sense Length: 25 AntiSense Length: 27

Sense MW: 7923.9 AntiSense MW: 8567.0

Total MW: 16491.0 Core GC%: 42.1

Asymmetrical End
2.62
Stability Difference:
Dengue 1 RNAi Silenceome
7

Sense Position: 2292 AntiSense Position: 2316

Sense Length: 25 AntiSense Length: 27

Sense MW: 8154.0 AntiSense MW: 8412.0

Total MW: 16566.1 Core GC%: 42.1

Asymmetrical End
3.47
Stability Difference:

Dengue 1 RNAi Silenceome

8

Sense Position: 10185 AntiSense Position: 10209

Sense Length: 25 AntiSense Length: 27

Sense MW: 8005.9 AntiSense MW: 8499.1

Total MW: 16505.0 Core GC%: 47.4

Asymmetrical End
3.4
Stability Difference:
Dengue 1 RNAi Silenceome
9

Sense Position: 1675 AntiSense Position: 1699

Sense Length: 25 AntiSense Length: 27

Sense MW: 8052.9 AntiSense MW: 8468.0

Total MW: 16521.0 Core GC%: 47.4

Asymmetrical End
3.89
Stability Difference:

Dengue 1 RNAi Silenceome

10

Sense Position: 8672 AntiSense Position: 8696

Sense Length: 25 AntiSense Length: 27

Sense MW: 8075.0 AntiSense MW: 8461.0

Total MW: 16536.0 Core GC%: 47.4

Asymmetrical End
5.76
Stability Difference:
Dengue 1 RNAi Silenceome
11

Sense Position: 5947 AntiSense Position: 5971

Sense Length: 25 AntiSense Length: 27

Sense MW: 8035.9 AntiSense MW: 8508.1

Total MW: 16544.0 Core GC%: 47.4

Asymmetrical End
4.73
Stability Difference:

Dengue 1 RNAi Silenceome

12

Sense Position: 7771 AntiSense Position: 7795

Sense Length: 25 AntiSense Length: 27

Sense MW: 8099.0 AntiSense MW: 8444.0

Total MW: 16543.0 Core GC%: 47.4

Asymmetrical End
3.72
Stability Difference:
Dengue 1 RNAi Silenceome
13

Sense Position: 4757 AntiSense Position: 4781

Sense Length: 25 AntiSense Length: 27

Sense MW: 8180.0 AntiSense MW: 8393.1

Total MW: 16573.1 Core GC%: 57.9

Asymmetrical End
4.5
Stability Difference:

Dengue 1 RNAi Silenceome

14

Sense Position: 6892 AntiSense Position: 6916

Sense Length: 25 AntiSense Length: 27

Sense MW: 7884.8 AntiSense MW: 8666.2

Total MW: 16551.0 Core GC%: 52.6

Asymmetrical End
4.62
Stability Difference:
Dengue 1 RNAi Silenceome
15

Sense Position: 7712 AntiSense Position: 7736

Sense Length: 25 AntiSense Length: 27

Sense MW: 8105.1 AntiSense MW: 8415.9

Total MW: 16521.0 Core GC%: 42.1

Asymmetrical End
4.34
Stability Difference:

Dengue 1 RNAi Silenceome

16

Sense Position: 6644 AntiSense Position: 6668

Sense Length: 25 AntiSense Length: 27

Sense MW: 7982.8 AntiSense MW: 8599.2

Total MW: 16582.1 Core GC%: 47.4

Asymmetrical End
5.51
Stability Difference:
Dengue 1 RNAi Silenceome
17

Details Blast Add to Order

Sense Position: 7823 AntiSense Position: 7847

Sense Length: 25 AntiSense Length: 27

Sense MW: 8023.8 AntiSense MW: 8496.2

Total MW: 16520.0 Core GC%: 47.4

Asymmetrical End
5.31
Stability Difference:

Dengue 1 RNAi Silenceome

18

Sense Position: 6406 AntiSense Position: 6430

Sense Length: 25 AntiSense Length: 27

Sense MW: 7940.9 AntiSense MW: 8573.1

Total MW: 16514.0 Core GC%: 42.1

Asymmetrical End
4.57
Stability Difference:
Dengue 1 RNAi Silenceome
19

Sense Position: 1469 AntiSense Position: 1493

Sense Length: 25 AntiSense Length: 27

Sense MW: 7927.8 AntiSense MW: 8617.2

Total MW: 16545.0 Core GC%: 42.1

Asymmetrical End
3.47
Stability Difference:

Dengue 1 RNAi Silenceome

20

Sense Position: 6784 AntiSense Position: 6808

Sense Length: 25 AntiSense Length: 27

Sense MW: 7975.8 AntiSense MW: 8567.2

Total MW: 16543.0 Core GC%: 47.4

Asymmetrical End
3.36
Stability Difference:
Dengue Virus Type 2

Set Overview

Site 1

Site 2

Site 3

Site 4

Site 5

Site 6

Site 7

Site 8

Site 9

Site 10

Site 11

Site 12

Site 13

Site 14

Site 15

Site 16

Site 17

Site 18

Site 19

Site 20

1 1000010723
Dengue Type 2 RNAi 1

Sense Position: 86 AntiSense Position: 110

Sense Length: 25 AntiSense Length: 27

Sense MW: 7956.9 AntiSense MW: 8548.1

Total MW: 16505.0 Core GC%: 36.8

Asymmetrical End
5.67
Stability Difference:

Dengue Type 2 RNAi 2

Sense Position: 10155 AntiSense Position: 10179

Sense Length: 25 AntiSense Length: 27

Sense MW: 7952.9 AntiSense MW: 8551.1

Total MW: 16504.0 Core GC%: 36.8

Asymmetrical End
4.53
Stability Difference:
Dengue Type 2 RNAi 3

Sense Position: 2902 AntiSense Position: 2926

Sense Length: 25 AntiSense Length: 27

Sense MW: 7933.8 AntiSense MW: 8618.2

Total MW: 16552.0 Core GC%: 42.1

Asymmetrical End
4.46
Stability Difference:

Dengue Type 2 RNAi 4

Sense Position: 6622 AntiSense Position: 6646

Sense Length: 25 AntiSense Length: 27

Sense MW: 8005.9 AntiSense MW: 8608.2

Total MW: 16614.1 Core GC%: 47.4

Asymmetrical End
5.24
Stability Difference:
Dengue Type 2 RNAi 5

Sense Position: 3435 AntiSense Position: 3459

Sense Length: 25 AntiSense Length: 27

Sense MW: 8178.1 AntiSense MW: 8373.0

Total MW: 16551.1 Core GC%: 36.8

Asymmetrical End
2.16
Stability Difference:

Dengue Type 2 RNAi 6

Sense Position: 1609 AntiSense Position: 1633

Sense Length: 25 AntiSense Length: 27

Sense MW: 8019.9 AntiSense MW: 8524.1

Total MW: 16544.0 Core GC%: 42.1

Asymmetrical End
5.05
Stability Difference:
Dengue Type 2 RNAi 7

Sense Position: 2238 AntiSense Position: 2262

Sense Length: 25 AntiSense Length: 27

Sense MW: 7901.8 AntiSense MW: 8649.2

Total MW: 16551.0 Core GC%: 52.6

Asymmetrical End
4.82
Stability Difference:

Dengue Type 2 RNAi 8

Sense Position: 7822 AntiSense Position: 7846

Sense Length: 25 AntiSense Length: 27

Sense MW: 8062.9 AntiSense MW: 8472.1

Total MW: 16535.0 Core GC%: 47.4

Asymmetrical End
5.51
Stability Difference:
Dengue Type 2 RNAi 9

Sense Position: 9105 AntiSense Position: 9129

Sense Length: 25 AntiSense Length: 27

Sense MW: 7957.9 AntiSense MW: 8595.2

Total MW: 16553.0 Core GC%: 42.1

Asymmetrical End
3.5
Stability Difference:

Dengue Type 2 RNAi 10

Sense Position: 3761 AntiSense Position: 3785

Sense Length: 25 AntiSense Length: 27

Sense MW: 7987.9 AntiSense MW: 8532.1

Total MW: 16520.0 Core GC%: 47.4

Asymmetrical End
4.38
Stability Difference:
Dengue Type 2 RNAi 11

Sense Position: 9319 AntiSense Position: 9343

Sense Length: 25 AntiSense Length: 27

Sense MW: 8042.0 AntiSense MW: 8518.0

Total MW: 16560.0 Core GC%: 47.4

Asymmetrical End
3.27
Stability Difference:

Dengue Type 2 RNAi 12

Sense Position: 8155 AntiSense Position: 8179

Sense Length: 25 AntiSense Length: 27

Sense MW: 8123.0 AntiSense MW: 8382.9

Total MW: 16506.0 Core GC%: 36.8

Asymmetrical End
4.21
Stability Difference:
Dengue Type 2 RNAi 13

Sense Position: 398 AntiSense Position: 422

Sense Length: 25 AntiSense Length: 27

Sense MW: 7941.8 AntiSense MW: 8601.2

Total MW: 16543.0 Core GC%: 42.1

Asymmetrical End
5.26
Stability Difference:

Dengue Type 2 RNAi 14

Sense Position: 7256 AntiSense Position: 7280

Sense Length: 25 AntiSense Length: 27

Sense MW: 7970.9 AntiSense MW: 8549.1

Total MW: 16520.0 Core GC%: 47.4

Asymmetrical End
3.23
Stability Difference:
Dengue Type 2 RNAi 15

Sense Position: 1100 AntiSense Position: 1124

Sense Length: 25 AntiSense Length: 27

Sense MW: 8012.9 AntiSense MW: 8547.1

Total MW: 16560.0 Core GC%: 42.1

Asymmetrical End
3.15
Stability Difference:

Dengue Type 2 RNAi 16

Sense Position: 8978 AntiSense Position: 9002

Sense Length: 25 AntiSense Length: 27

Sense MW: 8042.9 AntiSense MW: 8506.1

Total MW: 16549.0 Core GC%: 52.6

Asymmetrical End
2.09
Stability Difference:
Dengue Type 2 RNAi 17

Sense Position: 9699 AntiSense Position: 9723

Sense Length: 25 AntiSense Length: 27

Sense MW: 7869.8 AntiSense MW: 8682.2

Total MW: 16552.0 Core GC%: 36.8

Asymmetrical End
3.27
Stability Difference:

Dengue Type 2 RNAi 18

Sense Position: 274 AntiSense Position: 298

Sense Length: 25 AntiSense Length: 27

Sense MW: 8019.0 AntiSense MW: 8564.1

Total MW: 16583.1 Core GC%: 52.6

Asymmetrical End
4.99
Stability Difference:
Dengue Type 2 RNAi 19

Sense Position: 4314 AntiSense Position: 4338

Sense Length: 25 AntiSense Length: 27

Sense MW: 8138.0 AntiSense MW: 8420.0

Total MW: 16558.0 Core GC%: 52.6

Asymmetrical End
1.76
Stability Difference:

Dengue Type 2 RNAi 20

Sense Position: 6218 AntiSense Position: 6242

Sense Length: 25 AntiSense Length: 27

Sense MW: 7987.0 AntiSense MW: 8526.0

Total MW: 16513.0 Core GC%: 36.8

Asymmetrical End
3.95
Stability Difference:
Dengue Virus Type 3.

Set Overview

Site 1

Site 2

Site 3

Site 4

Site 5

Site 6

Site 7

Site 8

Site 9

Site 10

Site 11

Site 12

Site 13

Site 14

Site 15

Site 16

Site 17

Site 18

Site 19

Site 20

1 1000010707
Dengue Type 3 RNAi
Silenceome 1

Sense Position: 2011 AntiSense Position: 2035

Sense Length: 25 AntiSense Length: 27

Sense MW: 8059.9 AntiSense MW: 8490.1

Total MW: 16550.0 Core GC%: 47.4

Asymmetrical End
6.49
Stability Difference:

Dengue Type 3 RNAi

Silenceome 2

Sense Position: 8409 AntiSense Position: 8433

Sense Length: 25 AntiSense Length: 27

Sense MW: 8060.0 AntiSense MW: 8446.0

Total MW: 16506.0 Core GC%: 47.4

Asymmetrical End
3.59
Stability Difference:
Dengue Type 3 RNAi
Silenceome 3

Sense Position: 17 AntiSense Position: 41

Sense Length: 25 AntiSense Length: 27

Sense MW: 7985.9 AntiSense MW: 8586.2

Total MW: 16572.1 Core GC%: 47.4

Asymmetrical End
4.93
Stability Difference:

Dengue Type 3 RNAi

Silenceome 4

Sense Position: 6505 AntiSense Position: 6529

Sense Length: 25 AntiSense Length: 27

Sense MW: 8106.1 AntiSense MW: 8422.0

Total MW: 16528.0 Core GC%: 47.4

Asymmetrical End
4.74
Stability Difference:
Dengue Type 3 RNAi
Silenceome 5

Sense Position: 5534 AntiSense Position: 5558

Sense Length: 25 AntiSense Length: 27

Sense MW: 7971.9 AntiSense MW: 8587.1

Total MW: 16559.0 Core GC%: 47.4

Asymmetrical End
4.49
Stability Difference:

Dengue Type 3 RNAi

Silenceome 6

Sense Position: 7100 AntiSense Position: 7124

Sense Length: 25 AntiSense Length: 27

Sense MW: 7942.8 AntiSense MW: 8616.2

Total MW: 16559.0 Core GC%: 52.6

Asymmetrical End
5.43
Stability Difference:

Dengue Type 3 RNAi

Silenceome 7

Sense Position: 7814 AntiSense Position: 7838

Sense Length: 25 AntiSense Length: 27

Sense MW: 8060.9 AntiSense MW: 8473.1

Total MW: 16534.0 Core GC%: 47.4

Asymmetrical End
5.31
Stability Difference:

Dengue Type 3 RNAi

Silenceome 8

Sense Position: 3685 AntiSense Position: 3709

Sense Length: 25 AntiSense Length: 27

Sense MW: 7813.8 AntiSense MW: 8714.2

Total MW: 16528.0 Core GC%: 42.1

Asymmetrical End
5.4
Stability Difference:
Dengue Type 3 RNAi
Silenceome 9

Sense Position: 8901 AntiSense Position: 8925

Sense Length: 25 AntiSense Length: 27

Sense MW: 8005.9 AntiSense MW: 8522.2

Total MW: 16528.0 Core GC%: 42.1

Asymmetrical End
5.27
Stability Difference:

Dengue Type 3 RNAi

Silenceome 10

Sense Position: 6167 AntiSense Position: 6191

Sense Length: 25 AntiSense Length: 27

Sense MW: 8027.0 AntiSense MW: 8487.0

Total MW: 16514.0 Core GC%: 36.8

Asymmetrical End
3.91
Stability Difference:
Dengue Type 3 RNAi
Silenceome 11

Sense Position: 6871 AntiSense Position: 6895

Sense Length: 25 AntiSense Length: 27

Sense MW: 7918.8 AntiSense MW: 8632.2

Total MW: 16551.0 Core GC%: 52.6

Asymmetrical End
4.82
Stability Difference:

Dengue Type 3 RNAi

Silenceome 12

Sense Position: 5070 AntiSense Position: 5094

Sense Length: 25 AntiSense Length: 27

Sense MW: 7970.9 AntiSense MW: 8549.1

Total MW: 16520.0 Core GC%: 42.1

Asymmetrical End
5.75
Stability Difference:
Dengue Type 3 RNAi
Silenceome 13

Sense Position: 3474 AntiSense Position: 3498

Sense Length: 25 AntiSense Length: 27

Sense MW: 8051.0 AntiSense MW: 8469.0

Total MW: 16520.0 Core GC%: 47.4

Asymmetrical End
3.78
Stability Difference:

Dengue Type 3 RNAi

Silenceome 14

Sense Position: 3399 AntiSense Position: 3423

Sense Length: 25 AntiSense Length: 27

Sense MW: 8028.9 AntiSense MW: 8569.2

Total MW: 16598.1 Core GC%: 47.4

Asymmetrical End
5.56
Stability Difference:
Dengue Type 3 RNAi
Silenceome 15

Sense Position: 9694 AntiSense Position: 9718

Sense Length: 25 AntiSense Length: 27

Sense MW: 7845.8 AntiSense MW: 8721.3

Total MW: 16567.1 Core GC%: 42.1

Asymmetrical End
5.8
Stability Difference:

Dengue Type 3 RNAi

Silenceome 16

Sense Position: 2907 AntiSense Position: 2931

Sense Length: 25 AntiSense Length: 27

Sense MW: 7955.9 AntiSense MW: 8565.1

Total MW: 16521.0 Core GC%: 42.1

Asymmetrical End
2.36
Stability Difference:
Dengue Type 3 RNAi
Silenceome 17

Sense Position: 1444 AntiSense Position: 1468

Sense Length: 25 AntiSense Length: 27

Sense MW: 7909.8 AntiSense MW: 8610.2

Total MW: 16520.0 Core GC%: 42.1

Asymmetrical End
6.41
Stability Difference:

Dengue Type 3 RNAi

Silenceome 18

Sense Position: 4883 AntiSense Position: 4907

Sense Length: 25 AntiSense Length: 27

Sense MW: 7989.9 AntiSense MW: 8554.2

Total MW: 16544.0 Core GC%: 47.4

Asymmetrical End
3.47
Stability Difference:
Dengue Type 3 RNAi
Silenceome 19

Sense Position: 1379 AntiSense Position: 1403

Sense Length: 25 AntiSense Length: 27

Sense MW: 8098.0 AntiSense MW: 8438.0

Total MW: 16536.0 Core GC%: 47.4

Asymmetrical End
3.4
Stability Difference:

Dengue Type 3 RNAi

Silenceome 20

Sense Position: 7280 AntiSense Position: 7304

Sense Length: 25 AntiSense Length: 27

Sense MW: 7902.8 AntiSense MW: 8594.2

Total MW: 16497.0 Core GC%: 31.6

Asymmetrical End
5.47
Stability Difference:
Dengue Virus Type 4.

Set Overview

Site 1

Site 2

Site 3

Site 4

Site 5

Site 6

Site 7

Site 8

Site 9

Site 10

Site 11

Site 12

Site 13

Site 14

Site 15

Site 16

Site 17

Site 18

Site 19

Site 20

1 1000010649
Dengue Type 4 RNAi
Silenceome 1

Sense Position: 4874 AntiSense Position: 4898

Sense Length: 25 AntiSense Length: 27

Sense MW: 8035.9 AntiSense MW: 8485.0

Total MW: 16521.0 Core GC%: 42.1

Asymmetrical End
7.22
Stability Difference:

Dengue Type 4 RNAi

Silenceome 2

Sense Position: 1125 AntiSense Position: 1149

Sense Length: 25 AntiSense Length: 27

Sense MW: 7900.9 AntiSense MW: 8590.1

Total MW: 16491.0 Core GC%: 31.6

Asymmetrical End
5.64
Stability Difference:
Dengue Type 4 RNAi
Silenceome 3

Sense Position: 1315 AntiSense Position: 1339

Sense Length: 25 AntiSense Length: 27

Sense MW: 7997.9 AntiSense MW: 8493.1

Total MW: 16491.0 Core GC%: 42.1

Asymmetrical End
5.8
Stability Difference:

Dengue Type 4 RNAi

Silenceome 4

Sense Position: 2506 AntiSense Position: 2530

Sense Length: 25 AntiSense Length: 27

Sense MW: 7963.9 AntiSense MW: 8573.1

Total MW: 16537.0 Core GC%: 36.8

Asymmetrical End
6.07
Stability Difference:
Dengue Type 4 RNAi
Silenceome 5

Sense Position: 1379 AntiSense Position: 1403

Sense Length: 25 AntiSense Length: 27

Sense MW: 7978.9 AntiSense MW: 8541.1

Total MW: 16520.0 Core GC%: 47.4

Asymmetrical End
5.56
Stability Difference:

Dengue Type 4 RNAi

Silenceome 6

Sense Position: 9792 AntiSense Position: 9816

Sense Length: 25 AntiSense Length: 27

Sense MW: 8156.0 AntiSense MW: 8364.0

Total MW: 16520.0 Core GC%: 52.6

Asymmetrical End
5.84
Stability Difference:
Dengue Type 4 RNAi
Silenceome 7

Sense Position: 8031 AntiSense Position: 8055

Sense Length: 25 AntiSense Length: 27

Sense MW: 8153.1 AntiSense MW: 8374.0

Total MW: 16527.0 Core GC%: 36.8

Asymmetrical End
5.75
Stability Difference:

Dengue Type 4 RNAi

Silenceome 8

Sense Position: 2917 AntiSense Position: 2941

Sense Length: 25 AntiSense Length: 27

Sense MW: 7980.9 AntiSense MW: 8509.1

Total MW: 16490.0 Core GC%: 31.6

Asymmetrical End
4.65
Stability Difference:
Dengue Type 4 RNAi
Silenceome 9

Sense Position: 7812 AntiSense Position: 7836

Sense Length: 25 AntiSense Length: 27

Sense MW: 8070.9 AntiSense MW: 8433.1

Total MW: 16504.0 Core GC%: 42.1

Asymmetrical End
5.48
Stability Difference:

Dengue Type 4 RNAi

Silenceome 10

Sense Position: 3562 AntiSense Position: 3586

Sense Length: 25 AntiSense Length: 27

Sense MW: 7973.9 AntiSense MW: 8540.1

Total MW: 16514.0 Core GC%: 31.6

Asymmetrical End
4.29
Stability Difference:
Dengue Type 4 RNAi
Silenceome 11

Sense Position: 2283 AntiSense Position: 2307

Sense Length: 25 AntiSense Length: 27

Sense MW: 8093.9 AntiSense MW: 8458.1

Total MW: 16552.0 Core GC%: 52.6

Asymmetrical End
3.04
Stability Difference:

Dengue Type 4 RNAi

Silenceome 12

Sense Position: 6659 AntiSense Position: 6683

Sense Length: 25 AntiSense Length: 27

Sense MW: 7948.9 AntiSense MW: 8633.2

Total MW: 16582.1 Core GC%: 47.4

Asymmetrical End
6.04
Stability Difference:
Dengue Type 4 RNAi
Silenceome 13

Sense Position: 1891 AntiSense Position: 1915

Sense Length: 25 AntiSense Length: 27

Sense MW: 8114.0 AntiSense MW: 8428.0

Total MW: 16542.0 Core GC%: 47.4

Asymmetrical End
3.78
Stability Difference:

Dengue Type 4 RNAi

Silenceome 14

Sense Position: 4043 AntiSense Position: 4067

Sense Length: 25 AntiSense Length: 27

Sense MW: 7997.9 AntiSense MW: 8532.1

Total MW: 16530.0 Core GC%: 36.8

Asymmetrical End
4.73
Stability Difference:
Dengue Type 4 RNAi
Silenceome 15

Sense Position: 6942 AntiSense Position: 6966

Sense Length: 25 AntiSense Length: 27

Sense MW: 7882.8 AntiSense MW: 8690.2

Total MW: 16573.1 Core GC%: 52.6

Asymmetrical End
4.54
Stability Difference:

Dengue Type 4 RNAi

Silenceome 16

Sense Position: 7784 AntiSense Position: 7808

Sense Length: 25 AntiSense Length: 27

Sense MW: 8061.9 AntiSense MW: 8458.1

Total MW: 16520.0 Core GC%: 36.8

Asymmetrical End
4.49
Stability Difference:
Dengue Type 4 RNAi
Silenceome 17

Sense Position: 3902 AntiSense Position: 3926

Sense Length: 25 AntiSense Length: 27

Sense MW: 7913.8 AntiSense MW: 8650.2

Total MW: 16564.0 Core GC%: 52.6

Asymmetrical End
3.27
Stability Difference:

Dengue Type 4 RNAi

Silenceome 18

Sense Position: 4307 AntiSense Position: 4331

Sense Length: 25 AntiSense Length: 27

Sense MW: 8108.0 AntiSense MW: 8443.0

Total MW: 16551.0 Core GC%: 52.6

Asymmetrical End
5.06
Stability Difference:
Dengue Type 4 RNAi
Silenceome 19

Sense Position: 9137 AntiSense Position: 9161

Sense Length: 25 AntiSense Length: 27

Sense MW: 8044.9 AntiSense MW: 8475.1

Total MW: 16520.0 Core GC%: 42.1

Asymmetrical End
2.04
Stability Difference:

Dengue Type 4 RNAi

Silenceome 20

Sense Position: 1473 AntiSense Position: 1497

Sense Length: 25 AntiSense Length: 27

Sense MW: 7996.9 AntiSense MW: 8532.1

Total MW: 16529.0 Core GC%: 42.1

Asymmetrical End
3.3
Stability Difference:
 Toolbox
Brief Descriptions of Tools used
[1] CLC Sequence Viewer 6

This is a freeware package developed by CLC Bio, and consists of multiple tools to perform sequence

analysis, ranging from multiple sequence alignment to phylogenetic analysis, as well as other tasks

such as Restriction Mapping. This tool was employed during the course of this project to infer

phylogenetic relationships between the four serotypes of the Dengue virus and also carry out

multiple sequence alignment, both for purposes of establishing aforementioned phylogeny and for

purposes of obtaining broad spectrum consensus regions for serotype independent oligomer design.

The software can be obtained from http://www.clcbio.com/

IDT Scitools Suite

This set of online tools was used to design both the Phosphorothioate Oligomer panels and the Dicer

Substrate RNA Interference panels, this tool offers automatic integration with Genbank, facilitating

data processing through entry of the Genbank accession number alone. The tool offers a myriad of

design options and choices, the selected ones of which have been elaborated in the main paper.

The above is the interface of the Phosphorothioate antisense oligomer design tool, it can be

accessed online at http://eu.idtdna.com/Scitools/Applications/AntiSense/Antisense.aspx

The results derived from the use of this tool have already been presented earlier in the paper.
The above is an image of the interface of the Dicer Substrate RNAi design tool, and the tool itself can

be accessed at http://eu.idtdna.com/Scitools/Applications/RNAi/RNAi.aspx

// This is the end of the dissertation //