Research Article Isolation of Phytoconstituents from the leaves of Jasminum grandiflorum Linn ‘Sandeep and Vadmoa M Pasrakh* Department of Pharmacognosy, The Oxford College of Pharmacy, Bangalore-560 078, * For Correspondence: padmaparas(@hotmail.com ABSTRACT Fractionation of chloroform partitioned ethanol extract of the leavec of Jasminum _grandifioru Linn lead to the isolation of eatechin (1); Psiosterol (2), gall acid 3) and leenolc acid (4). Their stractures were elucidated by spectroscopic methods such as UV, IR,NMR and LOMS. All he compounds were isolated forthe first ime from this plant KEYWORDS Jasminum grandifiorum Lins Isolation; Psitosterol;eatechin;oleanolic cis eat acid; speewoscupicsvetd, INTRODUCTION Jasminum grandifiorum Linn var officinale (Oleaceae) is plant with fragrant ower, lrge serambling sub erect twining evergreen shrub cultivated both in the plains and on the hills ‘specially in India; Kashmi, Afghanistan, Persia, France, Italy, China, Japan, Morocco and Egypt’ Inthe Traditional System of Medicine, the leaves are useful in odontalgia, fixing loose tooth, Ulcerative stunnattis, Leprosy, skin diseases, cottorthoea, otalgia, strangury, dysmenorshoea, ulcers, wounds, ringworm and comms. The _hytoconstituens isolated so far from the leaves are sambacein LUI, 200-Epifraxamoside, demethyl- 200-cpifraxamoside, jasminanhydride, indole ‘oxgyensse kaempferob3-O-c-L shamnopyranosy] (1-93). a-L-themnopyranosyl (1->6)--D- ‘galactopsranosie, kaempferol3-O-rutinoside, 7- Ketologenin, oleoside-L1-metl ester, 7-glucosyl- methyl ester, ligstroside and oleurope lu the present work, we have isolted catechin (1), Bsitosterol (2), gallic acid (3) and oleanolic ert Heiage 1 (2) pean 208 a ‘cid (4) from the chloroform partitioned alcohol extract of dried leaves of Jasminum grandiflorun ‘All the compounds were isolated forthe first time ftom this pant. MATERIALS AND METHODS. Plant material Fresh leaves of Jasminum grandilorum were collested, shade dried and authonticated by Dr Siddamallayya. N, (RRUBNG/SMP/Drug authentication/2008-09/318), Central Council for Research in Ayurveda and Siddha, Bangalore. A ‘voucher specimen ofthe plant was deposited inthe Department of Pharmacognosy, The Oxford College of Pharmacy, Bangalore General instrument details ‘UV. Shimadzu UV VIS-1700; R-JASCO FTIR 5300; LCMS: Agilent 1100 LC-MSD APCI; 'H [NMR (00 Mia) and "C-NMR (125MH2) Bruker Avance S00, svvherbterngjural om ISSN 0914-9160stration and tsoation foribennann Bushrdestindeted he presence {County powdered leaves (450 gm) were ofutpenid compound IsIRspecran exited extracted with petroleum ether followed by chctrisicbansat 288em'erhyoxl oun ‘heofummanlsecolby he rocessofcontinous The 'H.NMR.as ella "C-NMR data we found ‘Ritnton olan). The rude etc wat o be Mdencal withthe spctum of thse already ‘ponte to dystonia capertr, reported earl for Psioser, 1 wis fae, Siihheporemage yc being 2.36% 126%and conte fy TLC and CO-TLC mod wih the {S67 Ym inter of ty pant materia Alcobol reference andar of Pio. ‘Gras na prgoned with chkroform a bo —-Compound 3 (JG:3) was isolated as white ‘hloroform and alcool extract were died. cyst; mp250-259C sR apectum exhibited ‘Chiwotorm pariioned faction ofthe alcool chaastestcban a 1703 cm forex! 0, xvas ms sujet o colin chromatography ban! at 366 mfr aromatic goup and a tro Creilengel(-120meshj using peoleun ether band a 3366emforhyony group, The "HANMER hylan) nde aeate(100 aswell C-NMR dt were found biel So. taking 250 mi fintion each time From withthe spectro hse aleay reported eae Frteceumeter:eiylaceate' 1 flcions 71-81 forglc acs. Ias te conimesby TLC ard Uo tyandactoneS1701 (17) pemlemeter. CO-TLC method withthe eferene standards of (ipl act: 32, ations 1118063) and gale ae ‘etl eer: ey awa: 11, actions 138- Compound 4 JG) was isolated as white 146 OG) on farer purification by factional amorphous powder, mp.:271-273C ls molecular Caystalization yielded compound 1(20 mg), forma was determined a C,H,0, onthe basis Shnpound 2 (05mg), compound 3 (06mg) and of MS. Is IR spectrum exhibited characteristic ‘oma (05 eapective nde ot 140% rent rou na broad ‘nt 400 fryers] oop. The HNMR [RESULTS AND DISCUSSION sells "C-NMR dt wee ound oe decal ‘The sractures of compound isolated were withthe spectrum of thse already reported caer ected on he tsi of pecrcep metiods fer Olemolicae. was fre eatined by TLC fdr given in Fig.l. an CO-TLC mod with th reference stands CongoundiUGetywastbonedasuiTeskved afelesoiei. Allie Composnd wee ated power mp. [SIZPC: I gave poste test for forthe fist tine oman, eno Hs IR spectrum exhibited characterise funds 3096 cfr erm rou anda road ACKNOWLEDGEMENT. andar 400cor! foros soup. The'H-NMIR The authors wish to think the Chima an Ilias "CNMR daawerefoundtobeldetcal Exceutve Diet, Children's Bsn Socixy hint spo those akeady scored earber andthe Oxford College of Piriany, Bese Teresa twas fre confimedby TLC and forte cities provided ferthisstidy Ms Natura CCO-TLE metiod wit he reference sands of Remedies Pvt Lt Bangatore and Mis Yuecn cate Enterprise, Mumbai forthe reference pi samples ‘Compound 2 (JG-2) was iolted as white of Catchin stosero and oleanoli acd mvnstots powder, mp: 139-14 Posie test ea eae 2) Ap 9 $2 eterno cn SNOMEDSTRUCTURES OF THE COMPOUNDS REFERENCES OBTAINED FROM JASMINEM 1. Anonymons, The Wealth find: ADictionary ‘GRANDIFLORUM ‘fin Rew Mates annual Poot, a, ‘ol 3 Pubioston nd lafomaton Directorate, (SiR, New Deli, 20042848 22 Kalam PH, Ansa, The Ayurvedic Pans 0. 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