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    Total Bilirubin Direct Bilirubin Pipette into well identified test tubes. Mix well and start a timer when adding specimen. Read absorbance at 550 nm against blanks.

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    Total Bilirubin Direct Bilirubin Pipette into well identified test tubes. Mix well and start a timer when adding specimen. Read absorbance at 550 nm against blanks.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    8 views3 pages

    Scores

    Uploaded by

    parthapady
    Total Bilirubin Direct Bilirubin Pipette into well identified test tubes. Mix well and start a timer when adding specimen. Read absorbance at 550 nm against blanks.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as XLSX, PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 3

    1 0

    2 0

    3 1
    Total Bilirubin Direct Bilirubin
    Pipette into well identified test tubes Blank Assay Blank
    Reagent R1 1 mL 1 mL
    Reagent R2 1 mL
    Distilled water 50 micro litre 50 micro litre
    Reagent R3 (Nitrite) 50 micro litre
    Mix
    Specimen 100 micro litre100 micro litre100 micro litre
    1. Mix well and start a timer when adding specimen. 2. Read absorbance at 550 nm against blanks.
    TB: reading after > 5 minutes at room temperature. DB: reading at exactly 5 minutes at room temperature.
    ect Bilirubin
    Assay

    1 mL

    50 microlitre

    100 micro litre


    nce at 550 nm against blanks.
    actly 5 minutes at room temperature.

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