Professional Documents
Culture Documents
October, 2010
What You Will Learn
- Prior vaccination with BCG not considered when interpreting PPD skin test.
It is impossible to differentiate between BCG mediated response and latent
infection.
– 90% of all persons infected with TB will never develop active TB
Source: CDC
Bacteria Exposure - Anthrax
From April 5, 2002 MMWR (Morbidity and Mortality Weekly Report from CDC)
Pertussis (whooping cough):
– Hazard is aerosol generation during
culture manipulation
– Incidence on the rise – see the l
atest report
– May be mild or classic in adults
– Most lab cases in research labs
- A pertussis vaccine is now
available for adults
Source: CDC
Brucellosis
– One of the most commonly reported
Source: CDC
lab-associated bacterial infection
• #1: 3 days before symptoms, the patient had prepared a gram stain
from the blood culture of a patient who was subsequently shown to
have meningococcal disease. The microbiologist had also handled and
sub-cultured agar plates w/ CSF. At this lab, aspiration from blood
culture bottles was performed at an open lab bench.
• #2: Microbiologist who worked at state public health lab and worked on
several n meningitides isolates performed slide agglutination tests.
Used BioSafety Level 2 precautions.
• Rickettsial Agents:
– Coxiella burnetti – Q fever
o High risk of lab infection
o Aerosol and parenteral exposures
– Rickettsia species
o Typhus, reported in 57 lab-associated cases
o Rocky mountain spotted fever, in 1976, 63 lab cases
were reported, 11 were fatal
o Aerosols and parenteral inoculation
CDC – lab management of agents associated with hantavirus – interim biosafety guid
-1994
Virus Exposure – Blood/Body Fluids
• Some pathogens are transmitted through
microorganisms contained in blood and other body
fluids.
• Examples are:
– Hepatitis B Virus (HBV)
– Hepatitis C Virus (HCV)
– Hepatitis D Virus (HDV)
– Human Immunodeficiency Virus (HIV)
• Blood includes:
– Human blood
– Human blood components, such as
packed cells and plasma
– Products made from human blood, such
as:
Clotting agents for hemophilia
Immune globulins including Rh
factor immune globulins
Virus Exposure – Blood/Body Fluids
• Hepatitis A and E:
– Fecally transmitted Hepatitis A virus
• Hepatitis B, C, and D
– Hepatitis B, active and passive vaccines available
– Hepatitis C, no vaccine available
– Hepatitis D, no vaccine available, however
immunization against hepatitis B also protects
against hepatitis D
Virus Exposure – Blood/Body Fluids
Human Immunodeficiency Virus (HIV)
– Attacks the human immune system
Parenteral
– Needlestick
– Scalpel/glass cut
• Mucous membrane
– Mouth pipetting
– Eating, drinking in lab area
– Not wearing appropriate PPE
– Non-intact skin
o Unguarded splash
o Contact with contaminated surfaces
o Not covering skin breaks
HIV Virus Exposure
Reference:
Surveillance of Occupationally Acquired HIV/AIDS in Healthcare Person
Lab Practices & Biosafety Guidelines
– Safety Equipment
– Laboratory Facilities