Donald Voet • Judith G. Voet • Charlotte W.

Pratt

Fundamentals of Biochemistry
Second Edition

Chapter 7:

Protein Function:
Myoglobin and Hemoglobin
Copyright © 2006 by John Wiley & Sons, Inc.
Structure Determines Function
Myoglobin (Mb)
Heme
Mb heme complex
Mb O2 binding curve
Hemoglobin
(Hb); deoxy
Hb: O2
Structural Differences
between 4° conform of
a) deoxyHb; b) oxyHb
O2 binding
curve Hb
Hill plot for Mb and purified
Movement of heme and
F helix from T (deoxy)
to R (oxy)
Change at 1-2
interface during T to
R
T to R changes
Network of ion pairs in deoxy (T)
Hbonds lost T to R
Bohr Effect
Role of Hb and Mb in O2 transport from lungs to respiring Mus
and CO2 (HCO3-) from tissues to lungs
Effect of
BPG and
CO2 on O2
dissociation
curve
Binding of
BPG to
deoxyHb
High altitude
adaptation
Symmetry model
of allosterism
Sequential model of allosterism
Structure of
deoxy S fiber
E6V of 2 to hydrophobic pocket 1